(Circular) (Six-base) MAPSORT of: pFN21AB7836.seq Check: 681 from: 1 to: 6541 pFN21AB7836 6541 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4351 278 Size: 53 83 186 936 2815 2468 Fragments arranged by size: 2815 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3884 1937 Size: 1947 4594 Acc65I G'GTAC_C Cuts at: 3862 3862 Size: 6541 AclI AA'CG_TT Cuts at: 1563 4668 5041 1563 Size: 3105 373 3063 Fragments arranged by size: 3105 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2824 2908 3160 3412 4604 5988 6256 1632 Size: 1192 84 252 252 1192 1384 268 1917 Fragments arranged by size: 1917 1384 1192 1192 268 252 252 84 AflII C'TTAA_G Cuts at: 820 1017 3343 820 Size: 197 2326 4018 Fragments arranged by size: 4018 2326 197 AflIII A'CryG_T Cuts at: 1203 2657 3665 5440 6417 1203 Size: 1454 1008 1775 977 1327 Fragments arranged by size: 1775 1454 1327 1008 977 AgeI A'CCGG_T Cuts at: 5372 5372 Size: 6541 AhdI GACnn_n'nnGTC Cuts at: 5270 5270 Size: 6541 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6541 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6541 AlwNI CAG_nnn'CTG Cuts at: 4284 5856 4284 Size: 1572 4969 ApaLI G'TGCA_C Cuts at: 4598 5754 4598 Size: 1156 5385 ApoI r'AATT_y Cuts at: 1493 2422 2489 2596 2602 3042 3126 3210 3378 Size: 929 67 107 6 440 84 84 168 Cuts at: 3378 3546 3714 3798 3850 4076 4228 6337 1493 Size: 168 168 84 52 226 152 2109 1697 Fragments arranged by size: 2109 1697 929 440 226 168 168 168 152 107 84 84 84 67 52 6 AseI AT'TA_AT Cuts at: 160 1032 5095 160 Size: 872 4063 1606 Fragments arranged by size: 4063 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6541 AvaI C'yCGr_G Cuts at: 1870 1943 3866 1870 Size: 73 1923 4545 Fragments arranged by size: 4545 1923 73 BamHI G'GATC_C Cuts at: 3871 4238 3871 Size: 367 6174 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3862 5317 618 Size: 325 217 627 2075 1455 1842 Fragments arranged by size: 2075 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3860 1391 Size: 2469 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2512 928 Size: 971 613 4957 Fragments arranged by size: 4957 971 613 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4732 4766 1903 Size: 34 2795 34 3678 Fragments arranged by size: 3678 2795 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4433 5643 4433 Size: 1210 5331 BclI T'GATC_A Cuts at: 1548 1548 Size: 6541 BfrBI ATG'CAT Cuts at: 3466 3749 3466 Size: 283 6258 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5152 136 Size: 107 122 71 631 605 3480 1525 Fragments arranged by size: 3480 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6536 6536 Size: 6541 BlpI GC'TnA_GC Cuts at: 3958 3958 Size: 6541 Bme1580I G_kGCm'C Cuts at: 1790 4602 5758 1790 Size: 2812 1156 2573 Fragments arranged by size: 2812 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2059 1318 Size: 741 5800 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2171 4272 5230 Size: 416 53 83 399 24 754 2101 958 Cuts at: 5230 442 Size: 1753 Fragments arranged by size: 2101 1753 958 754 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6541 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3207 3459 3207 Size: 252 6289 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2736 3156 3324 3408 3492 3576 3744 Size: 917 580 420 168 84 84 84 168 Cuts at: 3744 5201 1239 Size: 1457 2579 Fragments arranged by size: 2579 1457 917 580 420 168 168 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2952 4666 5531 5829 2952 Size: 1714 865 298 3664 Fragments arranged by size: 3664 1714 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2161 5204 882 Size: 617 662 3043 2219 Fragments arranged by size: 3043 2219 662 617 BsaAI yAC'GTr Cuts at: 493 1204 4332 493 Size: 711 3128 2702 Fragments arranged by size: 3128 2702 711 BsaBI GATnn'nnATC Cuts at: 6535 6535 Size: 6541 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2247 4348 4730 5365 Size: 53 83 186 936 714 2101 382 635 Cuts at: 5365 275 Size: 1451 Fragments arranged by size: 2101 1451 936 714 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3218 4974 5372 5646 5793 6204 3218 Size: 1756 398 274 147 411 3555 Fragments arranged by size: 3555 1756 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6316 Fragments arranged by size: 6316 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2242 1186 Size: 1056 5485 BseYI C'CCAG_C Cuts at: 2012 5744 6240 2012 Size: 3732 496 2313 Fragments arranged by size: 3732 2313 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3627 1163 Size: 573 1891 4077 Fragments arranged by size: 4077 1891 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3991 4752 5780 1540 Size: 275 186 1990 761 1028 2301 Fragments arranged by size: 2301 1990 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2070 2273 4602 4687 5758 2070 Size: 203 2329 85 1071 2853 Fragments arranged by size: 2853 2329 1071 203 85 BsmI GAATG_Cn' Cuts at: 3468 4052 4145 3468 Size: 584 93 5864 Fragments arranged by size: 5864 584 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2254 2254 Size: 6541 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2273 3860 4602 4687 5758 1391 Size: 399 280 203 1587 742 85 1071 2174 Fragments arranged by size: 2174 1587 1071 742 399 280 203 85 BspEI T'CCGG_A Cuts at: 6204 6204 Size: 6541 BspHI T'CATG_A Cuts at: 3370 4430 6160 3370 Size: 1060 1730 3751 Fragments arranged by size: 3751 1730 1060 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3896 844 Size: 3052 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3443 4428 1394 Size: 429 1620 985 3507 Fragments arranged by size: 3507 1620 985 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3247 5036 5210 65 Size: 1183 1999 1789 174 1396 Fragments arranged by size: 1999 1789 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5185 5372 1700 Size: 110 3375 187 2869 Fragments arranged by size: 3375 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6541 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6541 BssSI C'ACGA_G Cuts at: 4601 5613 4601 Size: 1012 5529 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6541 BstBI TT'CG_AA Cuts at: 5345 5345 Size: 6541 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6541 BstYI r'GATC_y Cuts at: 1972 2215 2372 3871 4238 4623 4640 6092 6178 Size: 243 157 1499 367 385 17 1452 86 Cuts at: 6178 6190 6536 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1499 1452 385 367 346 243 157 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5050 Fragments arranged by size: 5050 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3510 487 Size: 683 56 2284 3518 Fragments arranged by size: 3518 2284 683 56 BtsI GCAGTG_nn' Cuts at: 4057 4851 4871 4057 Size: 794 20 5727 Fragments arranged by size: 5727 794 20 ClaI AT'CG_AT Cuts at: 1554 3571 1554 Size: 2017 4524 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3229 3264 529 Size: 35 2665 35 3806 Fragments arranged by size: 3806 2665 35 35 DraI TTT'AAA Cuts at: 2731 2748 3151 3655 3845 2731 Size: 17 403 504 190 5427 Fragments arranged by size: 5427 504 403 190 17 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5548 809 Size: 507 4232 1802 Fragments arranged by size: 4232 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2494 3598 3988 Size: 54 1425 50 275 18 664 1104 390 Cuts at: 3988 4877 6506 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1104 889 664 390 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3988 1537 Size: 275 2176 4090 Fragments arranged by size: 4090 2176 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2382 3804 4471 1353 Size: 1029 1422 667 3423 Fragments arranged by size: 3423 1422 1029 667 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5128 5502 5648 387 Size: 1064 138 3539 374 146 1280 Fragments arranged by size: 3539 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2736 2824 2908 3156 3160 3324 Size: 393 524 580 88 84 248 4 164 Cuts at: 3324 3408 3412 3492 3576 3744 4604 5201 5988 Size: 84 4 80 84 168 860 597 787 Cuts at: 5988 6256 1239 Size: 268 1524 Fragments arranged by size: 1524 860 787 597 580 524 393 268 248 168 164 88 84 84 84 80 4 4 EcoO109I rG'GnC_Cy Cuts at: 2031 2874 2031 Size: 843 5698 EcoRI G'AATT_C Cuts at: 2596 3126 3210 3378 3546 3714 3850 4228 2596 Size: 530 84 168 168 168 136 378 4909 Fragments arranged by size: 4909 530 378 168 168 168 136 84 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3082 3082 Size: 6541 FspI TGC'GCA Cuts at: 5047 5047 Size: 6541 HaeII r_GCGC'y Cuts at: 1678 5688 1678 Size: 4010 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5182 5214 5256 5288 1373 Size: 32 135 32 3610 32 42 32 2626 Fragments arranged by size: 3610 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2292 3885 4131 669 Size: 988 281 354 1593 246 3079 Fragments arranged by size: 3079 1593 988 354 281 246 HindIII A'AGCT_T Cuts at: 748 2751 748 Size: 2003 4538 HpaI GTT'AAC Cuts at: 4131 4131 Size: 6541 KpnI G_GTAC'C Cuts at: 3866 3866 Size: 6541 MfeI C'AATT_G Cuts at: 4140 4140 Size: 6541 MluI A'CGCG_T Cuts at: 6417 6417 Size: 6541 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5655 5839 1305 Size: 4350 184 2007 Fragments arranged by size: 4350 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2496 3600 10 Size: 54 1425 343 664 1104 2951 Fragments arranged by size: 2951 1425 1104 664 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2481 2563 3403 3514 3571 4499 4858 Size: 648 1315 82 840 111 57 928 359 Cuts at: 4858 5017 518 Size: 159 2042 Fragments arranged by size: 2042 1315 928 840 648 359 159 111 82 57 MspA1I CmG'CkG Cuts at: 1606 2020 3957 4634 5782 6027 1606 Size: 414 1937 677 1148 245 2120 Fragments arranged by size: 2120 1937 1148 677 414 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5050 Fragments arranged by size: 5050 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6541 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6541 NotI GC'GGCC_GC Cuts at: 3988 3988 Size: 6541 NsiI A_TGCA'T Cuts at: 3468 3751 3468 Size: 283 6258 NspI r_CATG'y Cuts at: 1132 1229 2661 3669 3753 3839 3899 5444 1132 Size: 97 1432 1008 84 86 60 1545 2229 Fragments arranged by size: 2229 1545 1432 1008 97 86 84 60 PasI CC'CwG_GG Cuts at: 1348 2220 1348 Size: 872 5669 PciI A'CATG_T Cuts at: 2657 3665 5440 2657 Size: 1008 1775 3758 Fragments arranged by size: 3758 1775 1008 PflMI CCAn_nnn'nTGG Cuts at: 1108 2884 1108 Size: 1776 4765 PmeI GTTT'AAAC Cuts at: 3845 3845 Size: 6541 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2141 2173 4610 4642 6123 6155 2141 Size: 32 2437 32 1481 32 2527 Fragments arranged by size: 2527 2437 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2874 2874 Size: 6541 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6541 PsiI TTA'TAA Cuts at: 3795 4111 3795 Size: 316 6225 PstI C_TGCA'G Cuts at: 830 3893 830 Size: 3063 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6541 SalI G'TCGA_C Cuts at: 1936 3883 1936 Size: 1947 4594 SbfI CC_TGCA'GG Cuts at: 3893 3893 Size: 6541 ScaI AGT'ACT Cuts at: 4789 6501 4789 Size: 1712 4829 SexAI A'CCwGG_T Cuts at: 2151 2151 Size: 6541 SfcI C'TryA_G Cuts at: 826 1045 2143 2463 2896 3232 3889 5024 5705 Size: 219 1098 320 433 336 657 1135 681 Cuts at: 5705 5896 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1098 681 657 433 336 320 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6541 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6541 SmaI CCC'GGG Cuts at: 1872 3868 1872 Size: 1996 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2967 3343 4645 5546 5808 820 Size: 197 926 1024 376 1302 901 262 1553 Fragments arranged by size: 1553 1302 1024 926 901 376 262 197 SnaBI TAC'GTA Cuts at: 493 4332 493 Size: 3839 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6541 SphI G_CATG'C Cuts at: 1132 3899 1132 Size: 2767 3774 SspI AAT'ATT Cuts at: 5 52 4465 5 Size: 47 4413 2081 Fragments arranged by size: 4413 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6541 StyI C'CwwG_G Cuts at: 513 1065 2004 3980 513 Size: 552 939 1976 3074 Fragments arranged by size: 3074 1976 939 552 SwaI ATTT'AAAT Cuts at: 2731 2731 Size: 6541 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4568 4585 4738 4923 5368 325 Size: 1255 2988 17 153 185 445 1498 Fragments arranged by size: 2988 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2654 4787 6499 Size: 274 80 33 51 1443 677 2133 1712 Cuts at: 6499 96 Size: 138 Fragments arranged by size: 2133 1712 1443 677 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2669 2701 6501 6533 2669 Size: 32 3800 32 2677 Fragments arranged by size: 3800 2677 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2287 1316 Size: 971 5570 XbaI T'CTAG_A Cuts at: 3877 3877 Size: 6541 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6541 XmaI C'CCGG_G Cuts at: 1870 3866 1870 Size: 1996 4545 XmnI GAAnn'nnTTC Cuts at: 2837 3210 4670 2837 Size: 373 1460 4708 Fragments arranged by size: 4708 1460 373 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4349 276 Size: 53 83 186 936 2815 2468 Fragments arranged by size: 2815 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI XcmI