Restriction map for
pFN21AB7830
(Circular) (Six-base) MAPSORT of: pFN21AB7830.seq Check: 5188 from: 1 to: 6520
pFN21AB7830 6520 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4330 278
Size: 53 83 186 936 2794 2468
Fragments arranged by size:
2794 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3863 1937
Size: 1926 4594
Acc65I G'GTAC_C
Cuts at: 3841 3841
Size: 6520
AclI AA'CG_TT
Cuts at: 1563 4647 5020 1563
Size: 3084 373 3063
Fragments arranged by size:
3084 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2491 2908 2988 3160 3412 4583 5967 6235
Size: 859 417 80 172 252 1171 1384 268
Cuts at: 6235 1632
Size: 1917
Fragments arranged by size:
1917 1384 1171 859 417 268 252 172
80
AflII C'TTAA_G
Cuts at: 820 1017 3343 820
Size: 197 2326 3997
Fragments arranged by size:
3997 2326 197
AflIII A'CryG_T
Cuts at: 1203 3665 3749 5419 6396 1203
Size: 2462 84 1670 977 1327
Fragments arranged by size:
2462 1670 1327 977 84
AgeI A'CCGG_T
Cuts at: 5351 5351
Size: 6520
AhdI GACnn_n'nnGTC
Cuts at: 5249 5249
Size: 6520
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6520
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3028 1184
Size: 1844 4676
AlwNI CAG_nnn'CTG
Cuts at: 2323 4263 5835 2323
Size: 1940 1572 3008
Fragments arranged by size:
3008 1940 1572
ApaLI G'TGCA_C
Cuts at: 4577 5733 4577
Size: 1156 5364
ApoI r'AATT_y
Cuts at: 1493 2599 2958 3042 3126 3210 3378 3546 3714
Size: 1106 359 84 84 84 168 168 168
Cuts at: 3714 3798 3829 4055 4207 6316 1493
Size: 84 31 226 152 2109 1697
Fragments arranged by size:
2109 1697 1106 359 226 168 168 168
152 84 84 84 84 31
AseI AT'TA_AT
Cuts at: 160 1032 5074 160
Size: 872 4042 1606
Fragments arranged by size:
4042 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6520
AvaI C'yCGr_G
Cuts at: 1870 1943 2076 3845 1870
Size: 73 133 1769 4545
Fragments arranged by size:
4545 1769 133 73
BamHI G'GATC_C
Cuts at: 3850 4217 3850
Size: 367 6153
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3841 5296 618
Size: 325 217 627 2054 1455 1842
Fragments arranged by size:
2054 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2169 3839 1391
Size: 778 1670 4072
Fragments arranged by size:
4072 1670 778
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5549
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4711 4745 1903
Size: 34 2774 34 3678
Fragments arranged by size:
3678 2774 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4412 5622 4412
Size: 1210 5310
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6520
BfrBI ATG'CAT
Cuts at: 3466 3466
Size: 6520
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5131 136
Size: 107 122 71 631 605 3459 1525
Fragments arranged by size:
3459 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6515 6515
Size: 6520
BlpI GC'TnA_GC
Cuts at: 3937 3937
Size: 6520
Bme1580I G_kGCm'C
Cuts at: 1790 4581 5737 1790
Size: 2791 1156 2573
Fragments arranged by size:
2791 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6520
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2171 4251 5209
Size: 416 53 83 399 24 754 2080 958
Cuts at: 5209 442
Size: 1753
Fragments arranged by size:
2080 1753 958 754 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6520
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3207 3459 3207
Size: 252 6268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2820 3156 3324 3408 3492 3576 3744
Size: 917 664 336 168 84 84 84 168
Cuts at: 3744 5180 1239
Size: 1436 2579
Fragments arranged by size:
2579 1436 917 664 336 168 168 84
84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4645 5510 5808 4645
Size: 865 298 5357
Fragments arranged by size:
5357 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2161 5183 882
Size: 617 662 3022 2219
Fragments arranged by size:
3022 2219 662 617
BsaAI yAC'GTr
Cuts at: 493 1204 3698 4311 493
Size: 711 2494 613 2702
Fragments arranged by size:
2702 2494 711 613
BsaBI GATnn'nnATC
Cuts at: 6514 6514
Size: 6520
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2247 4327 4709 5344
Size: 53 83 186 936 714 2080 382 635
Cuts at: 5344 275
Size: 1451
Fragments arranged by size:
2080 1451 936 714 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3218 4953 5351 5625 5772 6183 3218
Size: 1735 398 274 147 411 3555
Fragments arranged by size:
3555 1735 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2151 2181 1178
Size: 30 165 30 748 30 5517
Fragments arranged by size:
5517 748 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2242 1186
Size: 1056 5464
BseYI C'CCAG_C
Cuts at: 2012 5723 6219 2012
Size: 3711 496 2313
Fragments arranged by size:
3711 2313 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5947
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3970 4731 5759 1540
Size: 275 186 1969 761 1028 2301
Fragments arranged by size:
2301 1969 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4581 4666 5737 4581
Size: 85 1071 5364
Fragments arranged by size:
5364 1071 85
BsmI GAATG_Cn'
Cuts at: 3468 4031 4124 3468
Size: 563 93 5864
Fragments arranged by size:
5864 563 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2254 2254
Size: 6520
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 3839 4581 4666 5737 1391
Size: 399 379 1670 742 85 1071 2174
Fragments arranged by size:
2174 1670 1071 742 399 379 85
BspEI T'CCGG_A
Cuts at: 6183 6183
Size: 6520
BspHI T'CATG_A
Cuts at: 4409 6139 4409
Size: 1730 4790
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3875 844
Size: 3031 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4407 1394
Size: 429 2584 3507
Fragments arranged by size:
3507 2584 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3247 5015 5189 65
Size: 1183 1999 1768 174 1396
Fragments arranged by size:
1999 1768 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5164 5351 1700
Size: 110 3354 187 2869
Fragments arranged by size:
3354 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6520
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6520
BssSI C'ACGA_G
Cuts at: 3371 4580 5592 3371
Size: 1209 1012 4299
Fragments arranged by size:
4299 1209 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6520
BstBI TT'CG_AA
Cuts at: 5324 5324
Size: 6520
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6520
BstYI r'GATC_y
Cuts at: 1972 2215 2336 3850 4217 4602 4619 6071 6157
Size: 243 121 1514 367 385 17 1452 86
Cuts at: 6157 6169 6515 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1514 1452 385 367 346 243 121
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5029
Fragments arranged by size:
5029 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5781
Fragments arranged by size:
5781 683 56
BtsI GCAGTG_nn'
Cuts at: 4036 4830 4850 4036
Size: 794 20 5706
Fragments arranged by size:
5706 794 20
ClaI AT'CG_AT
Cuts at: 1554 3571 1554
Size: 2017 4503
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3229 3264 529
Size: 35 2665 35 3785
Fragments arranged by size:
3785 2665 35 35
DraI TTT'AAA
Cuts at: 3151 3655 3824 3151
Size: 504 169 5847
Fragments arranged by size:
5847 504 169
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5527 809
Size: 507 4211 1802
Fragments arranged by size:
4211 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3598 3967 4856
Size: 54 1425 50 275 18 1768 369 889
Cuts at: 4856 6485 8
Size: 1629 43
Fragments arranged by size:
1768 1629 1425 889 369 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3967 1537
Size: 275 2155 4090
Fragments arranged by size:
4090 2155 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2261 2314 3804 4450 1353
Size: 908 53 1490 646 3423
Fragments arranged by size:
3423 1490 908 646 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5107 5481 5627 387
Size: 1064 138 3518 374 146 1280
Fragments arranged by size:
3518 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2491 2820 2908 2988 3156 3160
Size: 393 524 335 329 88 80 168 4
Cuts at: 3160 3324 3408 3412 3492 3576 3744 4583 5180
Size: 164 84 4 80 84 168 839 597
Cuts at: 5180 5967 6235 1239
Size: 787 268 1524
Fragments arranged by size:
1524 839 787 597 524 393 335 329
268 168 168 164 88 84 84 80
80 4 4
EcoO109I rG'GnC_Cy
Cuts at: 2031 3814 2031
Size: 1783 4737
EcoRI G'AATT_C
Cuts at: 2599 3042 3126 3210 3378 3546 3714 3829 4207
Size: 443 84 84 168 168 168 115 378
Cuts at: 4207 2599
Size: 4912
Fragments arranged by size:
4912 443 378 168 168 168 115 84
84
EcoRV GAT'ATC
Cuts at: 3591 3591
Size: 6520
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3082 3082
Size: 6520
FspI TGC'GCA
Cuts at: 5026 5026
Size: 6520
HaeII r_GCGC'y
Cuts at: 1678 5667 1678
Size: 3989 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3276 3308 5161 5193 5235
Size: 32 135 32 1704 32 1853 32 42
Cuts at: 5235 5267 1373
Size: 32 2626
Fragments arranged by size:
2626 1853 1704 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2292 3864 4110 669
Size: 988 281 354 1572 246 3079
Fragments arranged by size:
3079 1572 988 354 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6520
HpaI GTT'AAC
Cuts at: 4110 4110
Size: 6520
KpnI G_GTAC'C
Cuts at: 3845 3845
Size: 6520
MfeI C'AATT_G
Cuts at: 4119 4119
Size: 6520
MluI A'CGCG_T
Cuts at: 6396 6396
Size: 6520
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2364 5634 5818 1305
Size: 1059 3270 184 2007
Fragments arranged by size:
3270 2007 1059 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3600 10
Size: 54 1425 343 1768 2930
Fragments arranged by size:
2930 1768 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2566 3403 3514 3571 4478 4837 4996
Size: 648 1400 837 111 57 907 359 159
Cuts at: 4996 518
Size: 2042
Fragments arranged by size:
2042 1400 907 837 648 359 159 111
57
MspA1I CmG'CkG
Cuts at: 1606 2020 3936 4613 5761 6006 1606
Size: 414 1916 677 1148 245 2120
Fragments arranged by size:
2120 1916 1148 677 414 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5029
Fragments arranged by size:
5029 939 552
NdeI CA'TA_TG
Cuts at: 387 2816 387
Size: 2429 4091
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6520
NotI GC'GGCC_GC
Cuts at: 3967 3967
Size: 6520
NsiI A_TGCA'T
Cuts at: 3468 3468
Size: 6520
NspI r_CATG'y
Cuts at: 1132 1229 3669 3753 3878 5423 1132
Size: 97 2440 84 125 1545 2229
Fragments arranged by size:
2440 2229 1545 125 97 84
PasI CC'CwG_GG
Cuts at: 1348 2220 1348
Size: 872 5648
PciI A'CATG_T
Cuts at: 3665 3749 5419 3665
Size: 84 1670 4766
Fragments arranged by size:
4766 1670 84
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2884 1108
Size: 1776 4744
PmeI GTTT'AAAC
Cuts at: 3824 3824
Size: 6520
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2141 2173 4589 4621 6102 6134 2141
Size: 32 2416 32 1481 32 2527
Fragments arranged by size:
2527 2416 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3814 3814
Size: 6520
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6520
PsiI TTA'TAA
Cuts at: 2862 3318 3795 4090 2862
Size: 456 477 295 5292
Fragments arranged by size:
5292 477 456 295
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2728 2760 2728
Size: 32 6488
PstI C_TGCA'G
Cuts at: 830 3872 830
Size: 3042 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6520
SalI G'TCGA_C
Cuts at: 1936 3862 1936
Size: 1926 4594
SapI GCTCTTCn'nnn_
Cuts at: 2261 2261
Size: 6520
SbfI CC_TGCA'GG
Cuts at: 3872 3872
Size: 6520
ScaI AGT'ACT
Cuts at: 4768 6480 4768
Size: 1712 4808
SexAI A'CCwGG_T
Cuts at: 2151 2151
Size: 6520
SfcI C'TryA_G
Cuts at: 826 1045 2143 2466 2896 3232 3868 5003 5684
Size: 219 1098 323 430 336 636 1135 681
Cuts at: 5684 5875 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1098 681 636 430 336 323
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6520
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6520
SmaI CCC'GGG
Cuts at: 1872 3847 1872
Size: 1975 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3343 4624 5525 5787 820
Size: 197 926 1400 1281 901 262 1553
Fragments arranged by size:
1553 1400 1281 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4311 493
Size: 3818 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6520
SphI G_CATG'C
Cuts at: 1132 3878 1132
Size: 2746 3774
SspI AAT'ATT
Cuts at: 5 52 4444 5
Size: 47 4392 2081
Fragments arranged by size:
4392 2081 47
StuI AGG'CCT
Cuts at: 1517 2940 1517
Size: 1423 5097
StyI C'CwwG_G
Cuts at: 513 1065 2004 2354 3959 513
Size: 552 939 350 1605 3074
Fragments arranged by size:
3074 1605 939 552 350
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2462 4547 4564 4717 4902 5347 325
Size: 1255 882 2085 17 153 185 445 1498
Fragments arranged by size:
2085 1498 1255 882 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2849 4766 6478
Size: 274 80 33 51 1443 872 1917 1712
Cuts at: 6478 96
Size: 138
Fragments arranged by size:
1917 1712 1443 872 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2151 2183 6480 6512 2151
Size: 32 4297 32 2159
Fragments arranged by size:
4297 2159 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6520
XbaI T'CTAG_A
Cuts at: 3050 3856 3050
Size: 806 5714
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6520
XmaI C'CCGG_G
Cuts at: 1870 3845 1870
Size: 1975 4545
XmnI GAAnn'nnTTC
Cuts at: 3046 3210 3509 3761 4649 3046
Size: 164 299 252 888 4917
Fragments arranged by size:
4917 888 299 252 164
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4328 276
Size: 53 83 186 936 2794 2468
Fragments arranged by size:
2794 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PspOMI PspXI PvuII RsrII SacI
SacII SanDI SfiI SfoI SrfI SwaI XcmI