(Circular) (Six-base) MAPSORT of: pFN21AB7815.seq Check: 1242 from: 1 to: 6301 pFN21AB7815 6301 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4111 278 Size: 53 83 186 936 2575 2468 Fragments arranged by size: 2575 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3644 1937 Size: 1707 4594 Acc65I G'GTAC_C Cuts at: 3622 3622 Size: 6301 AclI AA'CG_TT Cuts at: 1563 4428 4801 1563 Size: 2865 373 3063 Fragments arranged by size: 3063 2865 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4364 5748 6016 1632 Size: 2732 1384 268 1917 Fragments arranged by size: 2732 1917 1384 268 AfeI AGC'GCT Cuts at: 2273 2273 Size: 6301 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6104 AflIII A'CryG_T Cuts at: 1203 2996 5200 6177 1203 Size: 1793 2204 977 1327 Fragments arranged by size: 2204 1793 1327 977 AgeI A'CCGG_T Cuts at: 5132 5132 Size: 6301 AhdI GACnn_n'nnGTC Cuts at: 5030 5030 Size: 6301 AleI CACnn'nnGTG Cuts at: 1166 3345 1166 Size: 2179 4122 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6301 AlwNI CAG_nnn'CTG Cuts at: 2365 4044 5616 2365 Size: 1679 1572 3050 Fragments arranged by size: 3050 1679 1572 ApaLI G'TGCA_C Cuts at: 4358 5514 4358 Size: 1156 5145 ApoI r'AATT_y Cuts at: 1493 2534 2934 3102 3610 3836 3988 6097 1493 Size: 1041 400 168 508 226 152 2109 1697 Fragments arranged by size: 2109 1697 1041 508 400 226 168 152 AseI AT'TA_AT Cuts at: 160 1032 4855 160 Size: 872 3823 1606 Fragments arranged by size: 3823 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 AvaI C'yCGr_G Cuts at: 1870 1943 3626 1870 Size: 73 1683 4545 Fragments arranged by size: 4545 1683 73 BamHI G'GATC_C Cuts at: 3631 3998 3631 Size: 367 5934 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3622 5077 618 Size: 325 217 627 1835 1455 1842 Fragments arranged by size: 1842 1835 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3620 1391 Size: 2229 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2999 928 Size: 971 1100 4230 Fragments arranged by size: 4230 1100 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4492 4526 1903 Size: 34 2555 34 3678 Fragments arranged by size: 3678 2555 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4193 5403 4193 Size: 1210 5091 BclI T'GATC_A Cuts at: 1548 2257 2671 3564 1548 Size: 709 414 893 4285 Fragments arranged by size: 4285 893 709 414 BfrBI ATG'CAT Cuts at: 2327 2686 2327 Size: 359 5942 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4912 136 Size: 107 122 71 631 605 3240 1525 Fragments arranged by size: 3240 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6296 6296 Size: 6301 BlpI GC'TnA_GC Cuts at: 3718 3718 Size: 6301 Bme1580I G_kGCm'C Cuts at: 1790 4362 5518 1790 Size: 2572 1156 2573 Fragments arranged by size: 2573 2572 1156 BmgBI CAC'GTC Cuts at: 1318 2997 1318 Size: 1679 4622 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3593 4032 4990 Size: 416 53 83 399 24 2176 439 958 Cuts at: 4990 442 Size: 1753 Fragments arranged by size: 2176 1753 958 439 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6301 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3385 3385 Size: 6301 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 2288 2719 4961 1239 Size: 815 234 431 2242 2579 Fragments arranged by size: 2579 2242 815 431 234 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2804 4426 5291 5589 2804 Size: 1622 865 298 3516 Fragments arranged by size: 3516 1622 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3089 3196 4964 882 Size: 617 1590 107 1768 2219 Fragments arranged by size: 2219 1768 1590 617 107 BsaAI yAC'GTr Cuts at: 493 1204 2399 4092 493 Size: 711 1195 1693 2702 Fragments arranged by size: 2702 1693 1195 711 BsaBI GATnn'nnATC Cuts at: 6295 6295 Size: 6301 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3035 4108 4490 5125 Size: 53 83 186 936 1502 1073 382 635 Cuts at: 5125 275 Size: 1451 Fragments arranged by size: 1502 1451 1073 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2164 2858 4734 5132 5406 5553 5964 2164 Size: 694 1876 398 274 147 411 2501 Fragments arranged by size: 2501 1876 694 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6076 Fragments arranged by size: 6076 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2078 3428 1186 Size: 892 1350 4059 Fragments arranged by size: 4059 1350 892 BseYI C'CCAG_C Cuts at: 5504 6000 5504 Size: 496 5805 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2647 3067 3507 1163 Size: 573 911 420 440 3957 Fragments arranged by size: 3957 911 573 440 420 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3751 4512 5540 1540 Size: 275 186 1750 761 1028 2301 Fragments arranged by size: 2301 1750 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4362 4447 5518 4362 Size: 85 1071 5145 Fragments arranged by size: 5145 1071 85 BsmI GAATG_Cn' Cuts at: 2798 3541 3812 3905 2798 Size: 743 271 93 5194 Fragments arranged by size: 5194 743 271 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3620 4362 4447 5518 1391 Size: 399 1830 742 85 1071 2174 Fragments arranged by size: 2174 1830 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5964 5964 Size: 6301 BspHI T'CATG_A Cuts at: 2314 2380 4190 5920 2314 Size: 66 1810 1730 2695 Fragments arranged by size: 2695 1810 1730 66 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3656 844 Size: 2812 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4188 1394 Size: 429 2365 3507 Fragments arranged by size: 3507 2365 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2344 2971 4796 4970 65 Size: 1183 1096 627 1825 174 1396 Fragments arranged by size: 1825 1396 1183 1096 627 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4945 5132 1700 Size: 110 3135 187 2869 Fragments arranged by size: 3135 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2923 96 Size: 2827 3474 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6301 BssSI C'ACGA_G Cuts at: 4361 5373 4361 Size: 1012 5289 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6301 BstBI TT'CG_AA Cuts at: 5105 5105 Size: 6301 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6301 BstXI CCAn_nnnn'nTGG Cuts at: 2032 2032 Size: 6301 BstYI r'GATC_y Cuts at: 1972 3631 3998 4383 4400 5852 5938 5950 6296 Size: 1659 367 385 17 1452 86 12 346 Cuts at: 6296 1972 Size: 1977 Fragments arranged by size: 1977 1659 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3493 3493 Size: 6301 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5749 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5562 Fragments arranged by size: 5562 683 56 BtsI GCAGTG_nn' Cuts at: 2148 2635 2887 3055 3546 3817 4611 4631 2148 Size: 487 252 168 491 271 794 20 3818 Fragments arranged by size: 3818 794 491 487 271 252 168 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6301 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2953 2988 529 Size: 35 2389 35 3842 Fragments arranged by size: 3842 2389 35 35 DraI TTT'AAA Cuts at: 3605 3605 Size: 6301 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5308 809 Size: 507 3992 1802 Fragments arranged by size: 3992 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3748 4637 6266 Size: 54 1425 50 275 18 1918 889 1629 Cuts at: 6266 8 Size: 43 Fragments arranged by size: 1918 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3748 1537 Size: 275 1936 4090 Fragments arranged by size: 4090 1936 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2180 4231 1353 Size: 827 2051 3423 Fragments arranged by size: 3423 2051 827 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4888 5262 5408 387 Size: 1064 138 3299 374 146 1280 Fragments arranged by size: 3299 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2288 2719 4364 4961 5748 6016 Size: 393 422 234 431 1645 597 787 268 Cuts at: 6016 1239 Size: 1524 Fragments arranged by size: 1645 1524 787 597 431 422 393 268 234 EcoO109I rG'GnC_Cy Cuts at: 2095 2095 Size: 6301 EcoRI G'AATT_C Cuts at: 2934 3102 3610 3988 2934 Size: 168 508 378 5247 Fragments arranged by size: 5247 508 378 168 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3055 3055 Size: 6301 FspI TGC'GCA Cuts at: 4807 4807 Size: 6301 HaeII r_GCGC'y Cuts at: 1678 2085 2275 5448 1678 Size: 407 190 3173 2531 Fragments arranged by size: 3173 2531 407 190 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4942 4974 5016 5048 1373 Size: 32 135 32 3370 32 42 32 2626 Fragments arranged by size: 3370 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2434 3645 3891 669 Size: 988 281 496 1211 246 3079 Fragments arranged by size: 3079 1211 988 496 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6301 HpaI GTT'AAC Cuts at: 2434 3891 2434 Size: 1457 4844 KpnI G_GTAC'C Cuts at: 3626 3626 Size: 6301 MfeI C'AATT_G Cuts at: 2208 3900 2208 Size: 1692 4609 MluI A'CGCG_T Cuts at: 6177 6177 Size: 6301 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2097 2163 5415 5599 1305 Size: 792 66 3252 184 2007 Fragments arranged by size: 3252 2007 792 184 66 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4479 Fragments arranged by size: 4479 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2319 2943 3345 4259 4618 4777 518 Size: 648 1153 624 402 914 359 159 2042 Fragments arranged by size: 2042 1153 914 648 624 402 359 159 MspA1I CmG'CkG Cuts at: 1606 3432 3717 4394 5542 5787 1606 Size: 1826 285 677 1148 245 2120 Fragments arranged by size: 2120 1826 1148 677 285 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5749 NdeI CA'TA_TG Cuts at: 387 2246 387 Size: 1859 4442 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6301 NotI GC'GGCC_GC Cuts at: 3748 3748 Size: 6301 NsiI A_TGCA'T Cuts at: 2329 2688 2329 Size: 359 5942 NspI r_CATG'y Cuts at: 1132 1229 3659 5204 1132 Size: 97 2430 1545 2229 Fragments arranged by size: 2430 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6301 PciI A'CATG_T Cuts at: 5200 5200 Size: 6301 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6301 PmeI GTTT'AAAC Cuts at: 3605 3605 Size: 6301 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4370 4402 5883 5915 4370 Size: 32 1481 32 4756 Fragments arranged by size: 4756 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2095 2095 Size: 6301 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6301 PsiI TTA'TAA Cuts at: 3871 3871 Size: 6301 PstI C_TGCA'G Cuts at: 830 3322 3653 830 Size: 2492 331 3478 Fragments arranged by size: 3478 2492 331 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6301 SalI G'TCGA_C Cuts at: 1936 3643 1936 Size: 1707 4594 SbfI CC_TGCA'GG Cuts at: 3653 3653 Size: 6301 ScaI AGT'ACT Cuts at: 4549 6261 4549 Size: 1712 4589 SexAI A'CCwGG_T Cuts at: 2133 2676 2133 Size: 543 5758 SfcI C'TryA_G Cuts at: 826 1045 2125 3318 3649 4784 5465 5656 826 Size: 219 1080 1193 331 1135 681 191 1471 Fragments arranged by size: 1471 1193 1135 1080 681 331 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6301 SmaI CCC'GGG Cuts at: 1872 3628 1872 Size: 1756 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2819 4405 5306 5568 820 Size: 197 926 876 1586 901 262 1553 Fragments arranged by size: 1586 1553 926 901 876 262 197 SnaBI TAC'GTA Cuts at: 493 4092 493 Size: 3599 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6301 SphI G_CATG'C Cuts at: 1132 3659 1132 Size: 2527 3774 SspI AAT'ATT Cuts at: 5 52 2338 4225 5 Size: 47 2286 1887 2081 Fragments arranged by size: 2286 2081 1887 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6301 StyI C'CwwG_G Cuts at: 513 1065 3300 3740 513 Size: 552 2235 440 3074 Fragments arranged by size: 3074 2235 552 440 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4328 4345 4498 4683 5128 325 Size: 1255 2748 17 153 185 445 1498 Fragments arranged by size: 2748 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2923 4547 6259 Size: 274 80 33 51 1443 946 1624 1712 Cuts at: 6259 96 Size: 138 Fragments arranged by size: 1712 1624 1443 946 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3459 3491 6261 6293 3459 Size: 32 2770 32 3467 Fragments arranged by size: 3467 2770 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6301 XbaI T'CTAG_A Cuts at: 3637 3637 Size: 6301 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6301 XmaI C'CCGG_G Cuts at: 1870 3626 1870 Size: 1756 4545 XmnI GAAnn'nnTTC Cuts at: 2517 2645 2729 4430 2517 Size: 128 84 1701 4388 Fragments arranged by size: 4388 1701 128 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4109 276 Size: 53 83 186 936 2575 2468 Fragments arranged by size: 2575 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI