(Circular) (Six-base) MAPSORT of: pFN21AB7857.seq Check: 192 from: 1 to: 6364 pFN21AB7857 6364 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2897 4174 278 Size: 53 83 186 936 1361 1277 2468 Fragments arranged by size: 2468 1361 1277 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3707 1937 Size: 1770 4594 Acc65I G'GTAC_C Cuts at: 3685 3685 Size: 6364 AclI AA'CG_TT Cuts at: 1563 4491 4864 1563 Size: 2928 373 3063 Fragments arranged by size: 3063 2928 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2869 3037 3205 4427 5811 6079 1632 Size: 1237 168 168 1222 1384 268 1917 Fragments arranged by size: 1917 1384 1237 1222 268 168 168 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6167 AflIII A'CryG_T Cuts at: 1203 2943 5263 6240 1203 Size: 1740 2320 977 1327 Fragments arranged by size: 2320 1740 1327 977 AgeI A'CCGG_T Cuts at: 5195 5195 Size: 6364 AhdI GACnn_n'nnGTC Cuts at: 5093 5093 Size: 6364 AleI CACnn'nnGTG Cuts at: 1166 2417 3096 1166 Size: 1251 679 4434 Fragments arranged by size: 4434 1251 679 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6364 AlwNI CAG_nnn'CTG Cuts at: 2641 3340 4107 5679 2641 Size: 699 767 1572 3326 Fragments arranged by size: 3326 1572 767 699 ApaI G_GGCC'C Cuts at: 2077 3635 2077 Size: 1558 4806 ApaLI G'TGCA_C Cuts at: 2598 3257 4421 5577 2598 Size: 659 1164 1156 3385 Fragments arranged by size: 3385 1164 1156 659 ApoI r'AATT_y Cuts at: 1493 3673 3899 4051 6160 1493 Size: 2180 226 152 2109 1697 Fragments arranged by size: 2180 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4918 160 Size: 872 3886 1606 Fragments arranged by size: 3886 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6364 AvaI C'yCGr_G Cuts at: 1870 1943 2397 2510 3628 3689 1870 Size: 73 454 113 1118 61 4545 Fragments arranged by size: 4545 1118 454 113 73 61 BamHI G'GATC_C Cuts at: 3507 3694 4061 3507 Size: 187 367 5810 Fragments arranged by size: 5810 367 187 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2345 2384 2994 3398 3685 Size: 325 217 627 558 39 610 404 287 Cuts at: 3685 5140 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 610 558 404 325 287 217 39 BanII G_rGCy'C Cuts at: 1391 2055 2077 2181 2724 2813 3233 3618 3635 Size: 664 22 104 543 89 420 385 17 Cuts at: 3635 3683 1391 Size: 48 4072 Fragments arranged by size: 4072 664 543 420 385 104 89 48 22 17 BbeI G_GCGC'C Cuts at: 3402 3402 Size: 6364 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2323 3530 928 Size: 971 424 1207 3762 Fragments arranged by size: 3762 1207 971 424 BbvCI CC'TCA_GC Cuts at: 3071 3071 Size: 6364 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2782 2816 3370 3404 4555 4589 1903 Size: 34 845 34 554 34 1151 34 3678 Fragments arranged by size: 3678 1151 845 554 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4256 5466 4256 Size: 1210 5154 BclI T'GATC_A Cuts at: 1548 1548 Size: 6364 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2072 2584 4975 Size: 107 122 71 631 605 400 512 2391 Cuts at: 4975 136 Size: 1525 Fragments arranged by size: 2391 1525 631 605 512 400 122 107 71 BglII A'GATC_T Cuts at: 6359 6359 Size: 6364 BlpI GC'TnA_GC Cuts at: 3410 3781 3410 Size: 371 5993 Bme1580I G_kGCm'C Cuts at: 1790 2077 2602 2951 3261 3635 4425 5581 1790 Size: 287 525 349 310 374 790 1156 2573 Fragments arranged by size: 2573 1156 790 525 374 349 310 287 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6364 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2184 2443 2863 Size: 416 53 83 399 24 767 259 420 Cuts at: 2863 4095 5053 442 Size: 1232 958 1753 Fragments arranged by size: 1753 1232 958 767 420 416 399 259 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6364 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2183 2269 2298 2663 5024 1239 Size: 944 86 29 365 2361 2579 Fragments arranged by size: 2579 2361 944 365 86 29 Bpu10I CC'TnA_GC Cuts at: 2455 2501 2525 2580 3071 2455 Size: 46 24 55 491 5748 Fragments arranged by size: 5748 491 55 46 24 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2807 4489 5354 5652 2807 Size: 1682 865 298 3519 Fragments arranged by size: 3519 1682 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5027 882 Size: 617 3528 2219 Fragments arranged by size: 3528 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4155 493 Size: 711 2951 2702 Fragments arranged by size: 2951 2702 711 BsaBI GATnn'nnATC Cuts at: 6358 6358 Size: 6364 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2517 2576 2894 2936 Size: 53 83 186 936 984 59 318 42 Cuts at: 2936 3103 3399 4171 4553 5188 275 Size: 167 296 772 382 635 1451 Fragments arranged by size: 1451 984 936 772 635 382 318 296 186 167 83 59 53 42 BsaWI w'CCGG_w Cuts at: 4797 5195 5469 5616 6027 4797 Size: 398 274 147 411 5134 Fragments arranged by size: 5134 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6139 Fragments arranged by size: 6139 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2057 2189 2696 2810 2971 3223 1186 Size: 871 132 507 114 161 252 4327 Fragments arranged by size: 4327 871 507 252 161 132 114 BseYI C'CCAG_C Cuts at: 2122 2557 2740 2759 3327 3634 5567 6063 2122 Size: 435 183 19 568 307 1933 496 2423 Fragments arranged by size: 2423 1933 568 496 435 307 183 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2700 2809 3326 3536 1163 Size: 573 964 109 517 210 3991 Fragments arranged by size: 3991 964 573 517 210 109 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2659 3015 3298 3814 4575 5603 Size: 275 186 658 356 283 516 761 1028 Cuts at: 5603 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 658 516 356 283 275 186 BsiHKAI G_wGCw'C Cuts at: 2602 2813 3261 4425 4510 5581 2602 Size: 211 448 1164 85 1071 3385 Fragments arranged by size: 3385 1164 1071 448 211 85 BsiWI C'GTAC_G Cuts at: 3170 3170 Size: 6364 BsmI GAATG_Cn' Cuts at: 2783 3423 3875 3968 2783 Size: 640 452 93 5179 Fragments arranged by size: 5179 640 452 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2927 3649 2927 Size: 722 5642 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2077 2181 2602 2724 2813 2951 Size: 399 265 22 104 421 122 89 138 Cuts at: 2951 3233 3261 3618 3635 3683 4425 4510 5581 Size: 282 28 357 17 48 742 85 1071 Cuts at: 5581 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 421 399 357 282 265 138 122 104 89 85 48 28 22 17 BspEI T'CCGG_A Cuts at: 6027 6027 Size: 6364 BspHI T'CATG_A Cuts at: 4253 5983 4253 Size: 1730 4634 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3719 844 Size: 2875 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2584 2851 3355 4251 1394 Size: 429 761 267 504 896 3507 Fragments arranged by size: 3507 896 761 504 429 267 BsrDI GCAATG_nn' Cuts at: 65 1248 4859 5033 65 Size: 1183 3611 174 1396 Fragments arranged by size: 3611 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2342 2651 3417 3515 5008 5195 1700 Size: 110 532 309 766 98 1493 187 2869 Fragments arranged by size: 2869 1493 766 532 309 187 110 98 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6364 BssHII G'CGCG_C Cuts at: 1925 3201 1925 Size: 1276 5088 BssSI C'ACGA_G Cuts at: 2145 2289 3164 4424 5436 2145 Size: 144 875 1260 1012 3073 Fragments arranged by size: 3073 1260 1012 875 144 BstAPI GCAn_nnn'nTGC Cuts at: 794 2554 2787 794 Size: 1760 233 4371 Fragments arranged by size: 4371 1760 233 BstBI TT'CG_AA Cuts at: 2775 5168 2775 Size: 2393 3971 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6364 BstXI CCAn_nnnn'nTGG Cuts at: 3098 3098 Size: 6364 BstYI r'GATC_y Cuts at: 1972 3087 3507 3694 4061 4446 4463 5915 6001 Size: 1115 420 187 367 385 17 1452 86 Cuts at: 6001 6013 6359 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1115 420 385 367 346 187 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2412 3058 513 Size: 552 939 408 646 3819 Fragments arranged by size: 3819 939 646 552 408 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5625 Fragments arranged by size: 5625 683 56 BtsI GCAGTG_nn' Cuts at: 2467 3464 3880 4674 4694 2467 Size: 997 416 794 20 4137 Fragments arranged by size: 4137 997 794 416 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6364 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6329 DraI TTT'AAA Cuts at: 3668 3668 Size: 6364 DraIII CAC_nnn'GTG Cuts at: 3125 3125 Size: 6364 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5371 809 Size: 507 4055 1802 Fragments arranged by size: 4055 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2285 2713 3012 Size: 54 1425 50 275 18 455 428 299 Cuts at: 3012 3127 3295 3463 3811 4700 6329 8 Size: 115 168 168 348 889 1629 43 Fragments arranged by size: 1629 1425 889 455 428 348 299 275 168 168 115 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3012 3295 3811 1537 Size: 275 1200 283 516 4090 Fragments arranged by size: 4090 1200 516 283 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2757 2801 3497 4294 1353 Size: 1404 44 696 797 3423 Fragments arranged by size: 3423 1404 797 696 44 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4951 5325 5471 387 Size: 1064 138 3362 374 146 1280 Fragments arranged by size: 3362 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2811 2811 Size: 6364 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2183 2269 2298 2663 2869 3037 3205 Size: 393 551 86 29 365 206 168 168 Cuts at: 3205 4427 5024 5811 6079 1239 Size: 1222 597 787 268 1524 Fragments arranged by size: 1524 1222 787 597 551 393 365 268 206 168 168 86 29 EcoNI CCTnn'n_nnAGG Cuts at: 2227 2227 Size: 6364 EcoO109I rG'GnC_Cy Cuts at: 2073 2074 2608 2638 2073 Size: 1 534 30 5799 Fragments arranged by size: 5799 534 30 1 EcoRI G'AATT_C Cuts at: 3673 4051 3673 Size: 378 5986 FspI TGC'GCA Cuts at: 3118 4870 3118 Size: 1752 4612 HaeII r_GCGC'y Cuts at: 1678 2634 3402 3484 5511 1678 Size: 956 768 82 2027 2531 Fragments arranged by size: 2531 2027 956 768 82 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5005 5037 5079 5111 1373 Size: 32 135 32 3433 32 42 32 2626 Fragments arranged by size: 3433 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3708 3954 669 Size: 988 281 1770 246 3079 Fragments arranged by size: 3079 1770 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6364 HpaI GTT'AAC Cuts at: 3954 3954 Size: 6364 KasI G'GCGC_C Cuts at: 3398 3398 Size: 6364 KpnI G_GTAC'C Cuts at: 3689 3689 Size: 6364 MfeI C'AATT_G Cuts at: 3963 3963 Size: 6364 MluI A'CGCG_T Cuts at: 2943 6240 2943 Size: 3297 3067 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2182 2838 3090 3342 5478 5662 1305 Size: 877 656 252 252 2136 184 2007 Fragments arranged by size: 2136 2007 877 656 252 252 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2287 2715 10 Size: 54 1425 343 455 428 3659 Fragments arranged by size: 3659 1425 455 428 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2417 3096 4322 4681 4840 518 Size: 648 1251 679 1226 359 159 2042 Fragments arranged by size: 2042 1251 1226 679 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2928 3030 3366 3656 3780 4457 5605 5850 Size: 1322 102 336 290 124 677 1148 245 Cuts at: 5850 1606 Size: 2120 Fragments arranged by size: 2120 1322 1148 677 336 290 245 124 102 NaeI GCC'GGC Cuts at: 2653 2653 Size: 6364 NarI GG'CG_CC Cuts at: 3399 3399 Size: 6364 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4873 Fragments arranged by size: 4873 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6364 NgoMIV G'CCGG_C Cuts at: 2651 2651 Size: 6364 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6364 NotI GC'GGCC_GC Cuts at: 3295 3811 3295 Size: 516 5848 NspI r_CATG'y Cuts at: 1132 1229 2552 3722 5267 1132 Size: 97 1323 1170 1545 2229 Fragments arranged by size: 2229 1545 1323 1170 97 PasI CC'CwG_GG Cuts at: 1348 2181 2612 1348 Size: 833 431 5100 Fragments arranged by size: 5100 833 431 PciI A'CATG_T Cuts at: 5263 5263 Size: 6364 PflMI CCAn_nnn'nTGG Cuts at: 1108 2641 1108 Size: 1533 4831 PfoI T'CCnGG_A Cuts at: 2055 2213 2246 2055 Size: 158 33 6173 Fragments arranged by size: 6173 158 33 PmeI GTTT'AAAC Cuts at: 3668 3668 Size: 6364 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4433 4465 5946 5978 4433 Size: 32 1481 32 4819 Fragments arranged by size: 4819 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2608 2638 2608 Size: 30 6334 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6364 PsiI TTA'TAA Cuts at: 3934 3934 Size: 6364 PspOMI G'GGCC_C Cuts at: 2073 3631 2073 Size: 1558 4806 PstI C_TGCA'G Cuts at: 830 2373 3716 830 Size: 1543 1343 3478 Fragments arranged by size: 3478 1543 1343 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6364 SacI G_AGCT'C Cuts at: 2813 2813 Size: 6364 SalI G'TCGA_C Cuts at: 1936 3706 1936 Size: 1770 4594 SapI GCTCTTCn'nnn_ Cuts at: 2757 2801 2757 Size: 44 6320 SbfI CC_TGCA'GG Cuts at: 3716 3716 Size: 6364 ScaI AGT'ACT Cuts at: 4612 6324 4612 Size: 1712 4652 SfcI C'TryA_G Cuts at: 826 1045 2224 2369 3712 4847 5528 5719 826 Size: 219 1179 145 1343 1135 681 191 1471 Fragments arranged by size: 1471 1343 1179 1135 681 219 191 145 SfoI GGC'GCC Cuts at: 3400 3400 Size: 6364 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6364 SgrAI Cr'CCGG_yG Cuts at: 1700 3417 1700 Size: 1717 4647 SmaI CCC'GGG Cuts at: 1872 3691 1872 Size: 1819 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2822 4468 5369 5631 820 Size: 197 926 879 1646 901 262 1553 Fragments arranged by size: 1646 1553 926 901 879 262 197 SnaBI TAC'GTA Cuts at: 493 4155 493 Size: 3662 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6364 SphI G_CATG'C Cuts at: 1132 2552 3722 1132 Size: 1420 1170 3774 Fragments arranged by size: 3774 1420 1170 SspI AAT'ATT Cuts at: 5 52 4288 5 Size: 47 4236 2081 Fragments arranged by size: 4236 2081 47 StuI AGG'CCT Cuts at: 1517 2506 2882 2966 3050 3386 1517 Size: 989 376 84 84 336 4495 Fragments arranged by size: 4495 989 376 336 84 84 StyI C'CwwG_G Cuts at: 513 1065 2004 3393 3803 513 Size: 552 939 1389 410 3074 Fragments arranged by size: 3074 1389 939 552 410 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2666 2852 3341 4391 4408 4561 4746 Size: 1255 1086 186 489 1050 17 153 185 Cuts at: 4746 5191 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1086 1050 489 445 186 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2028 4610 6322 Size: 274 80 33 51 1443 51 2582 1712 Cuts at: 6322 96 Size: 138 Fragments arranged by size: 2582 1712 1443 274 138 80 51 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2280 2312 2592 2624 6324 6356 2280 Size: 32 280 32 3700 32 2288 Fragments arranged by size: 3700 2288 280 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6364 XbaI T'CTAG_A Cuts at: 3700 3700 Size: 6364 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2165 2165 Size: 6364 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6364 XmaI C'CCGG_G Cuts at: 1870 3689 1870 Size: 1819 4545 XmnI GAAnn'nnTTC Cuts at: 2771 3023 3191 3275 3443 4493 2771 Size: 252 168 84 168 1050 4642 Fragments arranged by size: 4642 1050 252 168 168 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2895 4172 276 Size: 53 83 186 936 1361 1277 2468 Fragments arranged by size: 2468 1361 1277 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI