################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BanII BbeI BbvCI BcgI BciVI BfaI BglI BglII BlnI BlpI Bpu1102I BsaHI BsaI BsoBI BspDI BspHI BspLU11I BspMI BsrBI BsrDI BssHII Bst1107I BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DrdI Eam1105I EarI Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT14I FseI FspI Hin1I HpaI KasI KpnI Ksp632I KspI MaeI MfeI MluNI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PmeI PpuMI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StyI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccIII 860 TCCGGA T^CCGGA AflII 1297 CTTAAG C^TTAAG AflIII 1085 ACGCGT A^C[GA][CT]GT AlwNI 756 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG BamHI 1649 GGATCC G^GATCC BclI 924 TGATCA T^GATCA BfrI 1297 CTTAAG C^TTAAG BsaBI 1230 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 860 TCCGGA T^CCGGA BsiEI 945 CGGCCG CG[GA][CT]^CG BsiWI 1586 CGTACG C^GTACG BsmBI 1413 GAGACG CGTCTC(1/5) Bsp1407I 242 TGTACA T^GTACA BspEI 860 TCCGGA T^CCGGA BsrFI 475 GCCGGT [GA]^CCGG[CT] BsrGI 242 TGTACA T^GTACA BstBI 514 TTCGAA TT^CGAA BstYI 1649 GGATCC [GA]^GATC[CT] Cfr10I 475 GCCGGT [GA]^CCGG[CT] DsaI 1250 CCGTGG C^C[GA][CT]GG EaeI 945 CGGCCG [CT]^GGCC[GA] EagI 945 CGGCCG C^GGCCG EclXI 945 CGGCCG C^GGCCG Eco52I 945 CGGCCG C^GGCCG FbaI 924 TGATCA T^GATCA HincII 1542 GTCAAC GT[CT]^[GA]AC HindII 1542 GTCAAC GT[CT]^[GA]AC MamI 1230 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1649 GGATCC [GA]^GATC[CT] MluI 1085 ACGCGT A^CGCGT MroI 860 TCCGGA T^CCGGA MspA1I 1113 CCGCTG C[AC]G^C[GT]G NspV 514 TTCGAA TT^CGAA PshAI 835 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 837 CTGCAG CTGCA^G SfuI 514 TTCGAA TT^CGAA SmlI 1297 CTTAAG C^T[CT][GA]AG SspBI 242 TGTACA T^GTACA TaqI 515 TCGA T^CGA TthHB8I 515 TCGA T^CGA XhoII 1649 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcsI 64 GAATTT [GA]^AATT[CT] 102 GAATTT Alw44I 1089 GTGCAC G^TGCAC 1639 GTGCAC AlwI 1650 GATCC GGATC(4/5) 1649 GGATC AocI 792 CCTTAGG CC^T[ACGT]AGG 1716 CCTCAGG ApaLI 1089 GTGCAC G^TGCAC 1639 GTGCAC ApoI 64 GAATTT [GA]^AATT[CT] 102 GAATTT BbrPI 1054 CACGTG CAC^GTG 1726 CACGTG BsaAI 1054 CACGTG [CT]AC^GT[GA] 1726 CACGTG BsaWI 860 TCCGGA [AT]^CCGG[AT] 985 ACCGGA BssSI 696 CACGAG CACGAG(-5/-1) 1204 CTCGTG BstAPI 755 GCAGCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1091 GCACGCAGTGC Bsu36I 792 CCTTAGG CC^T[ACGT]AGG 1716 CCTCAGG DraII 574 AGGCCCT [GA]G^G[ACGT]CC[CT] 994 AGGCCCT Eco81I 792 CCTTAGG CC^T[ACGT]AGG 1716 CCTCAGG EcoO109I 574 AGGCCCT [GA]G^G[ACGT]CC[CT] 994 AGGCCCT EcoT22I 661 ATGCAT ATGCA^T 854 ATGCAT HaeII 361 GGCGCT [GA]GCGC^[CT] 373 GGCGCT HindIII 1022 AAGCTT A^AGCTT 1358 AAGCTT MseI 1130 TTAA T^TAA 1298 TTAA NsiI 661 ATGCAT ATGCA^T 854 ATGCAT PflMI 99 CCAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1149 CCACATGGTGG PmaCI 1054 CACGTG CAC^GTG 1726 CACGTG PmlI 1054 CACGTG CAC^GTG 1726 CACGTG SfcI 267 CTACAG C^T[GA][CT]AG 837 CTGCAG Tru9I 1130 TTAA T^TAA 1298 TTAA Van91I 99 CCAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1149 CCACATGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AccII AciI AfaI AluI Asp700I AspHI AvaII BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraIII EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI StuI TfiI TseI Tsp45I Tsp509I TspRI XmnI