(Circular) (Six-base) MAPSORT of: pFN21AA1588.seq Check: 182 from: 1 to: 6490 pFN21AA1588 6490 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4300 278 Size: 53 83 186 936 2764 2468 Fragments arranged by size: 2764 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3833 1937 Size: 1896 4594 Acc65I G'GTAC_C Cuts at: 3811 3811 Size: 6490 AclI AA'CG_TT Cuts at: 1563 4617 4990 1563 Size: 3054 373 3063 Fragments arranged by size: 3063 3054 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2775 3520 3575 3604 3688 4553 5937 6205 Size: 1143 745 55 29 84 865 1384 268 Cuts at: 6205 1632 Size: 1917 Fragments arranged by size: 1917 1384 1143 865 745 268 84 55 29 AflII C'TTAA_G Cuts at: 820 1017 3302 820 Size: 197 2285 4008 Fragments arranged by size: 4008 2285 197 AflIII A'CryG_T Cuts at: 1203 3090 5389 6366 1203 Size: 1887 2299 977 1327 Fragments arranged by size: 2299 1887 1327 977 AgeI A'CCGG_T Cuts at: 5321 5321 Size: 6490 AhdI GACnn_n'nnGTC Cuts at: 5219 5219 Size: 6490 AleI CACnn'nnGTG Cuts at: 1166 3018 1166 Size: 1852 4638 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6490 AlwNI CAG_nnn'CTG Cuts at: 2766 4233 5805 2766 Size: 1467 1572 3451 Fragments arranged by size: 3451 1572 1467 ApaLI G'TGCA_C Cuts at: 3094 3644 4547 5703 3094 Size: 550 903 1156 3881 Fragments arranged by size: 3881 1156 903 550 ApoI r'AATT_y Cuts at: 1493 2069 2107 3799 4025 4177 6286 1493 Size: 576 38 1692 226 152 2109 1697 Fragments arranged by size: 2109 1697 1692 576 226 152 38 AseI AT'TA_AT Cuts at: 160 1032 5044 160 Size: 872 4012 1606 Fragments arranged by size: 4012 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6490 AvaI C'yCGr_G Cuts at: 1870 1943 3815 1870 Size: 73 1872 4545 Fragments arranged by size: 4545 1872 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2279 2312 2279 Size: 33 6457 BamHI G'GATC_C Cuts at: 3654 3820 4187 3654 Size: 166 367 5957 Fragments arranged by size: 5957 367 166 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3811 5266 618 Size: 325 217 627 2024 1455 1842 Fragments arranged by size: 2024 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3809 1391 Size: 2418 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3173 3284 3442 3563 928 Size: 971 1274 111 158 121 3855 Fragments arranged by size: 3855 1274 971 158 121 111 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4681 4715 1903 Size: 34 2744 34 3678 Fragments arranged by size: 3678 2744 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4382 5592 4382 Size: 1210 5280 BclI T'GATC_A Cuts at: 1548 2929 1548 Size: 1381 5109 BfrBI ATG'CAT Cuts at: 2668 2861 2668 Size: 193 6297 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5101 136 Size: 107 122 71 631 605 3429 1525 Fragments arranged by size: 3429 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6485 6485 Size: 6490 BlpI GC'TnA_GC Cuts at: 3907 3907 Size: 6490 Bme1580I G_kGCm'C Cuts at: 1790 3098 3648 4551 5707 1790 Size: 1308 550 903 1156 2573 Fragments arranged by size: 2573 1308 1156 903 550 BmgBI CAC'GTC Cuts at: 1318 2034 1318 Size: 716 5774 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2145 2300 2451 Size: 416 53 83 399 24 728 155 151 Cuts at: 2451 4221 5179 442 Size: 1770 958 1753 Fragments arranged by size: 1770 1753 958 728 416 399 155 151 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6490 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2385 3773 2385 Size: 1388 5102 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2042 2156 2285 3348 3770 5150 1239 Size: 803 114 129 1063 422 1380 2579 Fragments arranged by size: 2579 1380 1063 803 422 129 114 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4615 5480 5778 4615 Size: 865 298 5327 Fragments arranged by size: 5327 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5153 882 Size: 617 3654 2219 Fragments arranged by size: 3654 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3061 3733 4281 493 Size: 711 1857 672 548 2702 Fragments arranged by size: 2702 1857 711 672 548 BsaBI GATnn'nnATC Cuts at: 3239 6484 3239 Size: 3245 3245 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4297 4679 5314 275 Size: 53 83 186 936 2764 382 635 1451 Fragments arranged by size: 2764 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2865 2990 4923 5321 5595 5742 6153 2865 Size: 125 1933 398 274 147 411 3202 Fragments arranged by size: 3202 1933 411 398 274 147 125 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3575 3605 1178 Size: 30 165 30 2172 30 4063 Fragments arranged by size: 4063 2172 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2354 2357 2372 1186 Size: 1168 3 15 5304 Fragments arranged by size: 5304 1168 15 3 BseYI C'CCAG_C Cuts at: 2312 5693 6189 2312 Size: 3381 496 2613 Fragments arranged by size: 3381 2613 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2146 2406 2779 1163 Size: 573 410 260 373 4874 Fragments arranged by size: 4874 573 410 373 260 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2953 3940 4701 5729 1540 Size: 275 186 952 987 761 1028 2301 Fragments arranged by size: 2301 1028 987 952 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3060 3098 3648 4551 4636 5707 3060 Size: 38 550 903 85 1071 3843 Fragments arranged by size: 3843 1071 903 550 85 38 BsiWI C'GTAC_G Cuts at: 3591 3591 Size: 6490 BsmI GAATG_Cn' Cuts at: 2861 3191 3434 3602 3686 4001 4094 2861 Size: 330 243 168 84 315 93 5257 Fragments arranged by size: 5257 330 315 243 168 93 84 BsmBI CGTCTCn'nnnn_ Cuts at: 3412 3412 Size: 6490 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3060 3098 3648 3809 4551 4636 5707 Size: 399 1270 38 550 161 742 85 1071 Cuts at: 5707 1391 Size: 2174 Fragments arranged by size: 2174 1270 1071 742 550 399 161 85 38 BspEI T'CCGG_A Cuts at: 2865 6153 2865 Size: 3288 3202 BspHI T'CATG_A Cuts at: 4379 6109 4379 Size: 1730 4760 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3845 844 Size: 3001 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4377 1394 Size: 429 2554 3507 Fragments arranged by size: 3507 2554 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4985 5159 65 Size: 1183 3737 174 1396 Fragments arranged by size: 3737 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2480 5134 5321 1700 Size: 110 670 2654 187 2869 Fragments arranged by size: 2869 2654 670 187 110 BsrGI T'GTAC_A Cuts at: 96 2247 96 Size: 2151 4339 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6490 BssSI C'ACGA_G Cuts at: 2701 3209 4550 5562 2701 Size: 508 1341 1012 3629 Fragments arranged by size: 3629 1341 1012 508 BstAPI GCAn_nnn'nTGC Cuts at: 794 2766 3102 794 Size: 1972 336 4182 Fragments arranged by size: 4182 1972 336 BstBI TT'CG_AA Cuts at: 2520 5294 2520 Size: 2774 3716 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6490 BstYI r'GATC_y Cuts at: 1972 3654 3820 4187 4572 4589 6041 6127 6139 Size: 1682 166 367 385 17 1452 86 12 Cuts at: 6139 6485 1972 Size: 346 1977 Fragments arranged by size: 1977 1682 1452 385 367 346 166 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2798 3722 2798 Size: 924 5566 BtgI C'CryG_G Cuts at: 513 1065 2004 3255 513 Size: 552 939 1251 3748 Fragments arranged by size: 3748 1251 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2953 487 Size: 683 56 1727 4024 Fragments arranged by size: 4024 1727 683 56 BtsI GCAGTG_nn' Cuts at: 2134 3107 3275 3527 4006 4800 4820 2134 Size: 973 168 252 479 794 20 3804 Fragments arranged by size: 3804 973 794 479 252 168 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6490 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6455 DraI TTT'AAA Cuts at: 3794 3794 Size: 6490 DraIII CAC_nnn'GTG Cuts at: 2153 3102 3160 2153 Size: 949 58 5483 Fragments arranged by size: 5483 949 58 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5497 809 Size: 507 4181 1802 Fragments arranged by size: 4181 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2950 3937 4826 Size: 54 1425 50 275 18 1120 987 889 Cuts at: 4826 6455 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1120 987 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2950 3937 1537 Size: 275 1138 987 4090 Fragments arranged by size: 4090 1138 987 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4420 1353 Size: 3067 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3487 3667 5077 5451 5597 387 Size: 1064 138 1898 180 1410 374 146 1280 Fragments arranged by size: 1898 1410 1280 1064 374 180 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2156 2285 2775 3348 3520 3575 Size: 393 410 114 129 490 573 172 55 Cuts at: 3575 3604 3688 3770 4553 5150 5937 6205 1239 Size: 29 84 82 783 597 787 268 1524 Fragments arranged by size: 1524 787 783 597 573 490 410 393 268 172 129 114 84 82 55 29 EcoO109I rG'GnC_Cy Cuts at: 2580 3000 2580 Size: 420 6070 EcoRI G'AATT_C Cuts at: 3799 4177 3799 Size: 378 6112 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3148 3148 Size: 6490 FspI TGC'GCA Cuts at: 4996 4996 Size: 6490 HaeII r_GCGC'y Cuts at: 1678 2370 2382 5637 1678 Size: 692 12 3255 2531 Fragments arranged by size: 3255 2531 692 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2387 2419 2569 2601 3197 Size: 32 135 32 815 32 150 32 596 Cuts at: 3197 3229 3449 3481 3701 3733 5131 5163 5205 Size: 32 220 32 220 32 1398 32 42 Cuts at: 5205 5237 1373 Size: 32 2626 Fragments arranged by size: 2626 1398 815 596 220 220 150 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3549 3834 4080 669 Size: 988 281 1611 285 246 3079 Fragments arranged by size: 3079 1611 988 285 281 246 HindIII A'AGCT_T Cuts at: 748 3027 3363 748 Size: 2279 336 3875 Fragments arranged by size: 3875 2279 336 HpaI GTT'AAC Cuts at: 4080 4080 Size: 6490 KpnI G_GTAC'C Cuts at: 3815 3815 Size: 6490 MfeI C'AATT_G Cuts at: 4089 4089 Size: 6490 MluI A'CGCG_T Cuts at: 3090 6366 3090 Size: 3276 3214 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3153 3657 5604 5788 1305 Size: 1848 504 1947 184 2007 Fragments arranged by size: 2007 1947 1848 504 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4668 Fragments arranged by size: 4668 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2645 3018 3343 3772 4448 4807 4966 Size: 648 1479 373 325 429 676 359 159 Cuts at: 4966 518 Size: 2042 Fragments arranged by size: 2042 1479 676 648 429 373 359 325 159 MspA1I CmG'CkG Cuts at: 1606 3120 3906 4583 5731 5976 1606 Size: 1514 786 677 1148 245 2120 Fragments arranged by size: 2120 1514 1148 786 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4999 Fragments arranged by size: 4999 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6490 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6490 NotI GC'GGCC_GC Cuts at: 3937 3937 Size: 6490 NsiI A_TGCA'T Cuts at: 2670 2863 2670 Size: 193 6297 NspI r_CATG'y Cuts at: 1132 1229 2650 2896 3568 3848 5393 1132 Size: 97 1421 246 672 280 1545 2229 Fragments arranged by size: 2229 1545 1421 672 280 246 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6490 PciI A'CATG_T Cuts at: 5389 5389 Size: 6490 PflMI CCAn_nnn'nTGG Cuts at: 1108 2110 3160 1108 Size: 1002 1050 4438 Fragments arranged by size: 4438 1050 1002 PfoI T'CCnGG_A Cuts at: 2058 3215 2058 Size: 1157 5333 PmeI GTTT'AAAC Cuts at: 3794 3794 Size: 6490 PmlI CAC'GTG Cuts at: 3061 3733 3061 Size: 672 5818 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4559 4591 6072 6104 4559 Size: 32 1481 32 4945 Fragments arranged by size: 4945 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2844 1613 Size: 1231 5259 PsiI TTA'TAA Cuts at: 4060 4060 Size: 6490 PstI C_TGCA'G Cuts at: 830 2846 3842 830 Size: 2016 996 3478 Fragments arranged by size: 3478 2016 996 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6490 SalI G'TCGA_C Cuts at: 1936 3832 1936 Size: 1896 4594 SbfI CC_TGCA'GG Cuts at: 3842 3842 Size: 6490 ScaI AGT'ACT Cuts at: 4738 6450 4738 Size: 1712 4778 SfcI C'TryA_G Cuts at: 826 1045 2272 2842 3838 4973 5654 5845 826 Size: 219 1227 570 996 1135 681 191 1471 Fragments arranged by size: 1471 1227 1135 996 681 570 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6490 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6490 SmaI CCC'GGG Cuts at: 1872 3817 1872 Size: 1945 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3302 4594 5495 5757 820 Size: 197 926 1359 1292 901 262 1553 Fragments arranged by size: 1553 1359 1292 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4281 493 Size: 3788 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6490 SphI G_CATG'C Cuts at: 1132 3848 1132 Size: 2716 3774 SspI AAT'ATT Cuts at: 5 52 4414 5 Size: 47 4362 2081 Fragments arranged by size: 4362 2081 47 StuI AGG'CCT Cuts at: 1517 2917 3533 3617 1517 Size: 1400 616 84 4390 Fragments arranged by size: 4390 1400 616 84 StyI C'CwwG_G Cuts at: 513 1065 2004 3929 513 Size: 552 939 1925 3074 Fragments arranged by size: 3074 1925 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2054 2164 4517 4534 4687 4872 5317 Size: 1255 474 110 2353 17 153 185 445 Cuts at: 5317 325 Size: 1498 Fragments arranged by size: 2353 1498 1255 474 445 185 153 110 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2247 4736 6448 Size: 274 80 33 51 1443 270 2489 1712 Cuts at: 6448 96 Size: 138 Fragments arranged by size: 2489 1712 1443 274 270 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6450 6482 6450 Size: 32 6458 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6490 XbaI T'CTAG_A Cuts at: 3826 3826 Size: 6490 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6490 XmaI C'CCGG_G Cuts at: 1870 3815 1870 Size: 1945 4545 XmnI GAAnn'nnTTC Cuts at: 2777 3029 3281 3365 3449 3617 3701 4619 2777 Size: 252 252 84 84 168 84 918 4648 Fragments arranged by size: 4648 918 252 252 168 84 84 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4298 276 Size: 53 83 186 936 2764 2468 Fragments arranged by size: 2764 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I BstXI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI