Restriction map for
pFN21AB7856
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccII AccIII AcyI
AflII AgeI AhdI Alw44I AlwI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AvaII AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BsaHI BseAI BsiWI BsmBI
BsmFI BsoBI BspDI BspEI BspMI
BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI BstXI
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraI DrdI Eam1105I Eco47III
EcoNI EcoO65I EcoRV EcoT22I FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PstI PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 351 GTATAC GT^[AC][GT]AC
AclI 1867 AACGTT AA^CGTT
AlwNI 343 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 238 CCTAAGG CC^T[ACGT]AGG
BanI 186 GGCACC G^G[CT][GA]CC
BclI 765 TGATCA T^GATCA
BcnI 24 CCCGG CC^[GC]GG
BsaAI 674 TACGTG [CT]AC^GT[GA]
BsaI 692 GAGACC GGTCTC(1/5)
BseRI 181 GAGGAG GAGGAG(10/8)
BsiEI 8 CGGCCG CG[GA][CT]^CG
BsmI 1726 GAATGC GAATGC(1/-1)
Bsp1407I 1353 TGTACA T^GTACA
BsrGI 1353 TGTACA T^GTACA
Bst1107I 351 GTATAC GTA^TAC
BstF5I 177 GGATG GGATG(2/0)
BstZ17I 351 GTATAC GTA^TAC
Bsu36I 238 CCTAAGG CC^T[ACGT]AGG
DraIII 118 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 336 CCATGG C^C[GA][CT]GG
EaeI 8 CGGCCG [CT]^GGCC[GA]
EagI 8 CGGCCG C^GGCCG
EarI 1697 GAAGAG CTCTTC(1/4)
EclXI 8 CGGCCG C^GGCCG
Eco52I 8 CGGCCG C^GGCCG
Eco81I 238 CCTAAGG CC^T[ACGT]AGG
FauI 216 GCGGG CCCGC(4/6)
FbaI 765 TGATCA T^GATCA
FokI 177 GGATG GGATG(9/13)
HindIII 653 AAGCTT A^AGCTT
HinfI 234 GATTC G^A[ACGT]TC
Ksp632I 1697 GAAGAG CTCTTC(1/4)
NciI 24 CCCGG CC^[GC]GG
NcoI 336 CCATGG C^CATGG
NdeI 1385 CATATG CA^TATG
Psp1406I 1867 AACGTT AA^CGTT
PvuII 471 CAGCTG CAG^CTG
SfaNI 126 GATGC GCATC(5/9)
SmlI 294 CTTGAG C^T[CT][GA]AG
SspBI 1353 TGTACA T^GTACA
TfiI 234 GATTC G^A[AT]TC
XcmI 593 CCAAGAGTGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BbsI 261 GAAGAC GAAGAC(2/6)
86 GTCTTC
BpuAI 261 GAAGAC GAAGAC(2/6)
86 GTCTTC
BspHI 1302 TCATGA T^CATGA
1311 TCATGA
BsrDI 443 GCAATG GCAATG(2/0)
1562 GCAATG
DraII 242 AGGCCCT [GA]G^G[ACGT]CC[CT]
1212 AGGGCCT
EcoO109I 242 AGGCCCT [GA]G^G[ACGT]CC[CT]
1212 AGGGCCT
EcoT14I 324 CCAAGG C^C[AT][AT]GG
336 CCATGG
MspA1I 214 CAGCGG C[AC]G^C[GT]G
471 CAGCTG
MwoI 405 GCACCAGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
443 GCAATGGGGGC
NlaIV 21 GGCCCC GG[ACGT]^[ACGT]CC
186 GGCACC
RcaI 1302 TCATGA T^CATGA
1311 TCATGA
SfcI 753 CTGTAG C^T[GA][CT]AG
825 CTATAG
StyI 324 CCAAGG C^C[AT][AT]GG
336 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
ApoI Asp700I AspHI BanII BbvI
BfaI BglII BmyI BpmI BsaJI
BsaWI BsgI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BspLU11I BsrI BssKI
BstNI BstYI Cfr13I DdeI DpnII
EcoRI EcoRII Fnu4HI HaeIII HapII
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI NdeII
NlaIII NspI RsaI SacI Sau3AI
Sau96I ScrFI StuI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmnI