(Circular) (Six-base) MAPSORT of: pFN21AB7856.seq Check: 9009 from: 1 to: 6586 pFN21AB7856 6586 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4396 278 Size: 53 83 186 936 2860 2468 Fragments arranged by size: 2860 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2357 3929 1937 Size: 420 1572 4594 Fragments arranged by size: 4594 1572 420 Acc65I G'GTAC_C Cuts at: 3907 3907 Size: 6586 AclI AA'CG_TT Cuts at: 1563 3873 4713 5086 1563 Size: 2310 840 373 3063 Fragments arranged by size: 3063 2310 840 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2425 2540 2775 4649 6033 6301 1632 Size: 793 115 235 1874 1384 268 1917 Fragments arranged by size: 1917 1874 1384 793 268 235 115 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6389 AflIII A'CryG_T Cuts at: 1203 2463 2674 2680 2721 2807 5485 6462 1203 Size: 1260 211 6 41 86 2678 977 1327 Fragments arranged by size: 2678 1327 1260 977 211 86 41 6 AgeI A'CCGG_T Cuts at: 5417 5417 Size: 6586 AhdI GACnn_n'nnGTC Cuts at: 5315 5315 Size: 6586 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6586 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2583 1184 Size: 1399 5187 AlwNI CAG_nnn'CTG Cuts at: 2353 4329 5901 2353 Size: 1976 1572 3038 Fragments arranged by size: 3038 1976 1572 ApaLI G'TGCA_C Cuts at: 4643 5799 4643 Size: 1156 5430 ApoI r'AATT_y Cuts at: 1493 2865 3033 3344 3789 3833 3895 4121 4273 Size: 1372 168 311 445 44 62 226 152 Cuts at: 4273 6382 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1372 445 311 226 168 152 62 44 AseI AT'TA_AT Cuts at: 160 1032 5140 160 Size: 872 4108 1606 Fragments arranged by size: 4108 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6586 AvaI C'yCGr_G Cuts at: 1870 1943 3911 1870 Size: 73 1968 4545 Fragments arranged by size: 4545 1968 73 BamHI G'GATC_C Cuts at: 3916 4283 3916 Size: 367 6219 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2191 3907 5362 618 Size: 325 217 627 404 1716 1455 1842 Fragments arranged by size: 1842 1716 1455 627 404 325 217 BanII G_rGCy'C Cuts at: 1391 2843 3599 3767 3905 1391 Size: 1452 756 168 138 4072 Fragments arranged by size: 4072 1452 756 168 138 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2084 2273 928 Size: 971 185 189 5241 Fragments arranged by size: 5241 971 189 185 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4777 4811 1903 Size: 34 2840 34 3678 Fragments arranged by size: 3678 2840 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4478 5688 4478 Size: 1210 5376 BclI T'GATC_A Cuts at: 1548 2770 1548 Size: 1222 5364 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5197 136 Size: 107 122 71 631 605 337 3188 1525 Fragments arranged by size: 3188 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2714 3260 3302 6581 2714 Size: 546 42 3279 2719 Fragments arranged by size: 3279 2719 546 42 BlpI GC'TnA_GC Cuts at: 4003 4003 Size: 6586 Bme1580I G_kGCm'C Cuts at: 1790 4647 5803 1790 Size: 2857 1156 2573 Fragments arranged by size: 2857 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2059 1318 Size: 741 5845 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2288 2650 4317 Size: 416 53 83 399 24 871 362 1667 Cuts at: 4317 5275 442 Size: 958 1753 Fragments arranged by size: 1753 1667 958 871 416 399 362 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6586 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2229 2229 Size: 6586 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2193 2895 2979 3315 3483 3567 3583 Size: 917 37 702 84 336 168 84 16 Cuts at: 3583 5246 1239 Size: 1663 2579 Fragments arranged by size: 2579 1663 917 702 336 168 84 84 37 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2320 4711 5576 5874 2320 Size: 2391 865 298 3032 Fragments arranged by size: 3032 2391 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2691 5249 882 Size: 617 1192 2558 2219 Fragments arranged by size: 2558 2219 1192 617 BsaAI yAC'GTr Cuts at: 493 1204 2681 4377 493 Size: 711 1477 1696 2702 Fragments arranged by size: 2702 1696 1477 711 BsaBI GATnn'nnATC Cuts at: 6580 6580 Size: 6586 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4393 4775 5410 275 Size: 53 83 186 936 2860 382 635 1451 Fragments arranged by size: 2860 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3195 3629 3713 5019 5417 5691 5838 6249 3195 Size: 434 84 1306 398 274 147 411 3532 Fragments arranged by size: 3532 1306 434 411 398 274 147 84 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2637 2667 1178 Size: 30 165 30 1234 30 5097 Fragments arranged by size: 5097 1234 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2201 1186 Size: 1015 5571 BseYI C'CCAG_C Cuts at: 5789 6285 5789 Size: 496 6090 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2464 2625 2746 2998 3082 3166 3418 Size: 573 728 161 121 252 84 84 252 Cuts at: 3418 3670 1163 Size: 252 4079 Fragments arranged by size: 4079 728 573 252 252 252 161 121 84 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2016 4036 4797 5825 1540 Size: 275 186 15 2020 761 1028 2301 Fragments arranged by size: 2301 2020 1028 761 275 186 15 BsiHKAI G_wGCw'C Cuts at: 2210 2843 3599 3767 4647 4732 5803 2210 Size: 633 756 168 880 85 1071 2993 Fragments arranged by size: 2993 1071 880 756 633 168 85 BsmI GAATG_Cn' Cuts at: 3737 4097 4190 3737 Size: 360 93 6133 Fragments arranged by size: 6133 360 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2210 2843 3599 3767 3905 4647 4732 Size: 399 420 633 756 168 138 742 85 Cuts at: 4732 5803 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 756 742 633 420 399 168 138 85 BspEI T'CCGG_A Cuts at: 6249 6249 Size: 6586 BspHI T'CATG_A Cuts at: 3307 3316 4475 6205 3307 Size: 9 1159 1730 3688 Fragments arranged by size: 3688 1730 1159 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3941 844 Size: 3097 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4473 1394 Size: 429 2650 3507 Fragments arranged by size: 3507 2650 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2455 3574 5081 5255 65 Size: 1183 1207 1119 1507 174 1396 Fragments arranged by size: 1507 1396 1207 1183 1119 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5230 5417 1700 Size: 110 3420 187 2869 Fragments arranged by size: 3420 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3358 96 Size: 3262 3324 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6586 BssSI C'ACGA_G Cuts at: 4646 5658 4646 Size: 1012 5574 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6586 BstBI TT'CG_AA Cuts at: 5390 5390 Size: 6586 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6586 BstYI r'GATC_y Cuts at: 1972 2714 3260 3302 3916 4283 4668 4685 6137 Size: 742 546 42 614 367 385 17 1452 Cuts at: 6137 6223 6235 6581 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 742 614 546 385 367 346 86 42 17 12 BstZ17I GTA'TAC Cuts at: 2358 2358 Size: 6586 Bsu36I CC'TnA_GG Cuts at: 2244 2244 Size: 6586 BtgI C'CryG_G Cuts at: 513 1065 2004 2341 513 Size: 552 939 337 4758 Fragments arranged by size: 4758 939 552 337 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5847 Fragments arranged by size: 5847 683 56 BtsI GCAGTG_nn' Cuts at: 2613 2734 2986 3070 3154 3406 3658 3742 4102 Size: 121 252 84 84 252 252 84 360 Cuts at: 4102 4896 4916 2613 Size: 794 20 4283 Fragments arranged by size: 4283 794 360 252 252 252 121 84 84 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6586 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3556 3591 529 Size: 35 2992 35 3524 Fragments arranged by size: 3524 2992 35 35 DraI TTT'AAA Cuts at: 3890 3890 Size: 6586 DraIII CAC_nnn'GTG Cuts at: 2128 2128 Size: 6586 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5593 809 Size: 507 4277 1802 Fragments arranged by size: 4277 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 4033 4922 Size: 54 1425 50 275 18 183 2020 889 Cuts at: 4922 6551 8 Size: 1629 43 Fragments arranged by size: 2020 1629 1425 889 275 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2013 4033 1537 Size: 275 201 2020 4090 Fragments arranged by size: 4090 2020 275 201 EarI CTCTTCn'nnn_ Cuts at: 1353 3697 4516 1353 Size: 2344 819 3423 Fragments arranged by size: 3423 2344 819 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5173 5547 5693 387 Size: 1064 138 3584 374 146 1280 Fragments arranged by size: 3584 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2841 3597 3765 2841 Size: 756 168 5662 Fragments arranged by size: 5662 756 168 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2193 2425 2540 2775 2895 2979 Size: 393 524 37 232 115 235 120 84 Cuts at: 2979 3315 3483 3567 3583 4649 5246 6033 6301 Size: 336 168 84 16 1066 597 787 268 Cuts at: 6301 1239 Size: 1524 Fragments arranged by size: 1524 1066 787 597 524 393 336 268 235 232 168 120 115 84 84 37 16 EcoO109I rG'GnC_Cy Cuts at: 2248 3218 2248 Size: 970 5616 EcoRI G'AATT_C Cuts at: 2865 3033 3789 3895 4273 2865 Size: 168 756 106 378 5178 Fragments arranged by size: 5178 756 378 168 106 FspI TGC'GCA Cuts at: 5092 5092 Size: 6586 HaeII r_GCGC'y Cuts at: 1678 5733 1678 Size: 4055 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5227 5259 5301 5333 1373 Size: 32 135 32 3655 32 42 32 2626 Fragments arranged by size: 3655 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3930 4176 669 Size: 988 281 1992 246 3079 Fragments arranged by size: 3079 1992 988 281 246 HindIII A'AGCT_T Cuts at: 748 2658 748 Size: 1910 4676 HpaI GTT'AAC Cuts at: 4176 4176 Size: 6586 KpnI G_GTAC'C Cuts at: 3911 3911 Size: 6586 MfeI C'AATT_G Cuts at: 4185 4185 Size: 6586 MluI A'CGCG_T Cuts at: 6462 6462 Size: 6586 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2407 3298 3641 3876 5700 5884 1305 Size: 1102 891 343 235 1824 184 2007 Fragments arranged by size: 2007 1824 1102 891 343 235 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4764 Fragments arranged by size: 4764 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2679 2892 3321 3394 4544 4903 5062 Size: 648 1513 213 429 73 1150 359 159 Cuts at: 5062 518 Size: 2042 Fragments arranged by size: 2042 1513 1150 648 429 359 213 159 73 MspA1I CmG'CkG Cuts at: 1606 2221 2478 4002 4679 5827 6072 1606 Size: 615 257 1524 677 1148 245 2120 Fragments arranged by size: 2120 1524 1148 677 615 257 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2341 513 Size: 552 939 337 4758 Fragments arranged by size: 4758 939 552 337 NdeI CA'TA_TG Cuts at: 387 3391 387 Size: 3004 3582 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6586 NotI GC'GGCC_GC Cuts at: 4033 4033 Size: 6586 NspI r_CATG'y Cuts at: 1132 1229 2373 2426 2467 2678 2725 2811 3944 Size: 97 1144 53 41 211 47 86 1133 Cuts at: 3944 5489 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 1144 1133 211 97 86 53 47 41 PasI CC'CwG_GG Cuts at: 1348 2035 2161 1348 Size: 687 126 5773 Fragments arranged by size: 5773 687 126 PciI A'CATG_T Cuts at: 2463 2674 2721 2807 5485 2463 Size: 211 47 86 2678 3564 Fragments arranged by size: 3564 2678 211 86 47 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6586 PfoI T'CCnGG_A Cuts at: 2075 2075 Size: 6586 PmeI GTTT'AAAC Cuts at: 3890 3890 Size: 6586 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4655 4687 6168 6200 4655 Size: 32 1481 32 5041 Fragments arranged by size: 5041 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6586 PsiI TTA'TAA Cuts at: 4156 4156 Size: 6586 PstI C_TGCA'G Cuts at: 830 3938 830 Size: 3108 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6586 PvuII CAG'CTG Cuts at: 2478 2478 Size: 6586 SacI G_AGCT'C Cuts at: 2843 3599 3767 2843 Size: 756 168 5662 Fragments arranged by size: 5662 756 168 SalI G'TCGA_C Cuts at: 1936 3928 1936 Size: 1992 4594 SbfI CC_TGCA'GG Cuts at: 3938 3938 Size: 6586 ScaI AGT'ACT Cuts at: 4834 6546 4834 Size: 1712 4874 SfcI C'TryA_G Cuts at: 826 1045 2758 2830 3934 5069 5750 5941 826 Size: 219 1713 72 1104 1135 681 191 1471 Fragments arranged by size: 1713 1471 1135 1104 681 219 191 72 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6586 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6586 SmaI CCC'GGG Cuts at: 1872 3913 1872 Size: 2041 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2299 4690 5591 5853 820 Size: 197 926 356 2391 901 262 1553 Fragments arranged by size: 2391 1553 926 901 356 262 197 SnaBI TAC'GTA Cuts at: 493 4377 493 Size: 3884 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6586 SphI G_CATG'C Cuts at: 1132 3944 1132 Size: 2812 3774 SspI AAT'ATT Cuts at: 5 52 4510 5 Size: 47 4458 2081 Fragments arranged by size: 4458 2081 47 StuI AGG'CCT Cuts at: 1517 2968 3052 3136 3472 3556 3640 3724 3808 Size: 1451 84 84 336 84 84 84 84 Cuts at: 3808 1517 Size: 4295 Fragments arranged by size: 4295 1451 336 84 84 84 84 84 84 StyI C'CwwG_G Cuts at: 513 1065 2004 2329 2341 4025 513 Size: 552 939 325 12 1684 3074 Fragments arranged by size: 3074 1684 939 552 325 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2153 4613 4630 4783 4968 5413 325 Size: 1255 573 2460 17 153 185 445 1498 Fragments arranged by size: 2460 1498 1255 573 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3358 4832 6544 Size: 274 80 33 51 1443 1381 1474 1712 Cuts at: 6544 96 Size: 138 Fragments arranged by size: 1712 1474 1443 1381 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2637 2669 6546 6578 2637 Size: 32 3877 32 2645 Fragments arranged by size: 3877 2645 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6586 XbaI T'CTAG_A Cuts at: 3922 3922 Size: 6586 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2605 2605 Size: 6586 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6586 XmaI C'CCGG_G Cuts at: 1870 3911 1870 Size: 2041 4545 XmnI GAAnn'nnTTC Cuts at: 2438 2504 2531 2660 4715 2438 Size: 66 27 129 2055 4309 Fragments arranged by size: 4309 2055 129 66 27 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4394 276 Size: 53 83 186 936 2860 2468 Fragments arranged by size: 2860 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI