Restriction map for
pFN21AB7856
(Linear) (Six-base) MAPSORT of: KIBB7856.seq Check: 2333 from: 1 to: 1881
KIBB7856.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 0 352 1881
Size: 352 1529
AclI AA'CG_TT
Cuts at: 0 1868 1881
Size: 1868 13
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 535 770 1881
Size: 420 115 235 1111
Fragments arranged by size:
1111 420 235 115
AflIII A'CryG_T
Cuts at: 0 458 669 675 716 802 1881
Size: 458 211 6 41 86 1079
Fragments arranged by size:
1079 458 211 86 41 6
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 578 1881
Size: 578 1303
AlwNI CAG_nnn'CTG
Cuts at: 0 348 1881
Size: 348 1533
ApoI r'AATT_y
Cuts at: 0 860 1028 1339 1784 1828 1881
Size: 860 168 311 445 44 53
Fragments arranged by size:
860 445 311 168 53 44
BanI G'GyrC_C
Cuts at: 0 186 1881
Size: 186 1695
BanII G_rGCy'C
Cuts at: 0 838 1594 1762 1881
Size: 838 756 168 119
Fragments arranged by size:
838 756 168 119
BbsI GAAGACnn'nnnn_
Cuts at: 0 79 268 1881
Size: 79 189 1613
Fragments arranged by size:
1613 189 79
BclI T'GATC_A
Cuts at: 0 765 1881
Size: 765 1116
BglII A'GATC_T
Cuts at: 0 709 1255 1297 1881
Size: 709 546 42 584
Fragments arranged by size:
709 584 546 42
BmgBI CAC'GTC
Cuts at: 0 54 1881
Size: 54 1827
BmrI ACTGGGnnnn_n'
Cuts at: 0 283 645 1881
Size: 283 362 1236
Fragments arranged by size:
1236 362 283
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 224 1881
Size: 224 1657
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 188 890 974 1310 1478 1562 1578
Size: 151 37 702 84 336 168 84 16
Cuts at: 1578 1881
Size: 303
Fragments arranged by size:
702 336 303 168 151 84 84 37
16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 1881
Size: 315 1566
BsaI GGTCTCn'nnnn_
Cuts at: 0 686 1881
Size: 686 1195
BsaAI yAC'GTr
Cuts at: 0 676 1881
Size: 676 1205
BsaWI w'CCGG_w
Cuts at: 0 1190 1624 1708 1881
Size: 1190 434 84 173
Fragments arranged by size:
1190 434 173 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 632 662 1881
Size: 632 30 1219
Fragments arranged by size:
1219 632 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 196 1881
Size: 196 1685
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 459 620 741 993 1077 1161 1413 1665
Size: 459 161 121 252 84 84 252 252
Cuts at: 1665 1881
Size: 216
Fragments arranged by size:
459 252 252 252 216 161 121 84
84
BsiEI CG_ry'CG
Cuts at: 0 11 1881
Size: 11 1870
BsiHKAI G_wGCw'C
Cuts at: 0 205 838 1594 1762 1881
Size: 205 633 756 168 119
Fragments arranged by size:
756 633 205 168 119
BsmI GAATG_Cn'
Cuts at: 0 1732 1881
Size: 1732 149
Bsp1286I G_dGCh'C
Cuts at: 0 205 838 1594 1762 1881
Size: 205 633 756 168 119
Fragments arranged by size:
756 633 205 168 119
BspHI T'CATG_A
Cuts at: 0 1302 1311 1881
Size: 1302 9 570
Fragments arranged by size:
1302 570 9
BsrDI GCAATG_nn'
Cuts at: 0 450 1569 1881
Size: 450 1119 312
Fragments arranged by size:
1119 450 312
BsrGI T'GTAC_A
Cuts at: 0 1353 1881
Size: 1353 528
BstYI r'GATC_y
Cuts at: 0 709 1255 1297 1881
Size: 709 546 42 584
Fragments arranged by size:
709 584 546 42
BstZ17I GTA'TAC
Cuts at: 0 353 1881
Size: 353 1528
Bsu36I CC'TnA_GG
Cuts at: 0 239 1881
Size: 239 1642
BtgI C'CryG_G
Cuts at: 0 336 1881
Size: 336 1545
BtsI GCAGTG_nn'
Cuts at: 0 608 729 981 1065 1149 1401 1653 1737
Size: 608 121 252 84 84 252 252 84
Cuts at: 1737 1881
Size: 144
Fragments arranged by size:
608 252 252 252 144 121 84 84
84
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1551 1586 1881
Size: 1551 35 295
Fragments arranged by size:
1551 295 35
DraIII CAC_nnn'GTG
Cuts at: 0 123 1881
Size: 123 1758
EaeI y'GGCC_r
Cuts at: 0 8 1881
Size: 8 1873
EagI C'GGCC_G
Cuts at: 0 8 1881
Size: 8 1873
EarI CTCTTCn'nnn_
Cuts at: 0 1692 1881
Size: 1692 189
EcoICRI GAG'CTC
Cuts at: 0 836 1592 1760 1881
Size: 836 756 168 121
Fragments arranged by size:
836 756 168 121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 188 420 535 770 890 974 1310
Size: 151 37 232 115 235 120 84 336
Cuts at: 1310 1478 1562 1578 1881
Size: 168 84 16 303
Fragments arranged by size:
336 303 235 232 168 151 120 115
84 84 37 16
EcoO109I rG'GnC_Cy
Cuts at: 0 243 1213 1881
Size: 243 970 668
Fragments arranged by size:
970 668 243
EcoRI G'AATT_C
Cuts at: 0 860 1028 1784 1881
Size: 860 168 756 97
Fragments arranged by size:
860 756 168 97
HindIII A'AGCT_T
Cuts at: 0 653 1881
Size: 653 1228
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 402 1293 1636 1871 1881
Size: 402 891 343 235 10
Fragments arranged by size:
891 402 343 235 10
MslI CAynn'nnrTG
Cuts at: 0 674 887 1316 1389 1881
Size: 674 213 429 73 492
Fragments arranged by size:
674 492 429 213 73
MspA1I CmG'CkG
Cuts at: 0 216 473 1881
Size: 216 257 1408
Fragments arranged by size:
1408 257 216
NcoI C'CATG_G
Cuts at: 0 336 1881
Size: 336 1545
NdeI CA'TA_TG
Cuts at: 0 1386 1881
Size: 1386 495
NspI r_CATG'y
Cuts at: 0 368 421 462 673 720 806 1881
Size: 368 53 41 211 47 86 1075
Fragments arranged by size:
1075 368 211 86 53 47 41
PasI CC'CwG_GG
Cuts at: 0 30 156 1881
Size: 30 126 1725
Fragments arranged by size:
1725 126 30
PciI A'CATG_T
Cuts at: 0 458 669 716 802 1881
Size: 458 211 47 86 1079
Fragments arranged by size:
1079 458 211 86 47
PfoI T'CCnGG_A
Cuts at: 0 70 1881
Size: 70 1811
PvuII CAG'CTG
Cuts at: 0 473 1881
Size: 473 1408
SacI G_AGCT'C
Cuts at: 0 838 1594 1762 1881
Size: 838 756 168 119
Fragments arranged by size:
838 756 168 119
SfcI C'TryA_G
Cuts at: 0 753 825 1881
Size: 753 72 1056
Fragments arranged by size:
1056 753 72
SmlI C'TyrA_G
Cuts at: 0 294 1881
Size: 294 1587
StuI AGG'CCT
Cuts at: 0 963 1047 1131 1467 1551 1635 1719 1803
Size: 963 84 84 336 84 84 84 84
Cuts at: 1803 1881
Size: 78
Fragments arranged by size:
963 336 84 84 84 84 84 84
78
StyI C'CwwG_G
Cuts at: 0 324 336 1881
Size: 324 12 1545
Fragments arranged by size:
1545 324 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 148 1881
Size: 148 1733
TatI w'GTAC_w
Cuts at: 0 1353 1881
Size: 1353 528
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 632 664 1881
Size: 632 32 1217
Fragments arranged by size:
1217 632 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 600 1881
Size: 600 1281
XmnI GAAnn'nnTTC
Cuts at: 0 433 499 526 655 1881
Size: 433 66 27 129 1226
Fragments arranged by size:
1226 433 129 66 27
Enzymes that do cut:
AccI AclI AcuI AflIII AlfI AlwNI ApoI BanI
BanII BbsI BclI BglII BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaWI BsaXI BseRI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrGI BstYI BstZ17I
Bsu36I BtgI BtsI CspCI DraIII EaeI EagI EarI
EcoICRI Eco57MI EcoO109I EcoRI HindIII MmeI MslI MspA1I
NcoI NdeI NspI PasI PciI PfoI PvuII SacI
SfcI SmlI StuI StyI TaqII TatI TstI XcmI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BglI
BlpI Bme1580I BmtI Bpu10I BsaBI BsaHI BseYI BsiWI
BsmBI BspEI BspMI BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BtgZI ClaI DraI DrdI EciI
EcoNI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HincII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XhoI XmaI ZraI