Restriction map for
pFN21AB7853
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AviII BalI BamHI BbeI BbrPI
BbvCI BcgI BcnI BfrI BglII
BlpI Bpu1102I BsaAI BsaHI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FauI FseI FspI HaeII HgaI
Hin1I HpaI KasI KpnI KspI
MaeII MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsaI RsrII
SacII SalI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SspBI SspI SwaI Tth111I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Alw44I 1311 GTGCAC G^TGCAC
ApaLI 1311 GTGCAC G^TGCAC
AvaI 1596 CTCGAG C^[CT]CG[GA]G
AvrII 24 CCTAGG C^CTAGG
BanI 480 GGTGCC G^G[CT][GA]CC
BbsI 1242 GAAGAC GAAGAC(2/6)
BciVI 392 GTATCC GTATCC(6/5)
BclI 617 TGATCA T^GATCA
BglI 1201 GCCCCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 24 CCTAGG C^CTAGG
BpuAI 1242 GAAGAC GAAGAC(2/6)
BsaBI 387 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1291 ACCGGA [AT]^CCGG[AT]
BsoBI 1596 CTCGAG C^[CT]CG[GA]G
BspHI 165 TCATGA T^CATGA
BsrDI 381 GCAATG GCAATG(2/0)
BssSI 1444 CACGAG CACGAG(-5/-1)
BstAPI 1313 GCACCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 1437 GCGC GCG^C
DsaI 1205 CCATGG C^C[GA][CT]GG
FbaI 617 TGATCA T^GATCA
HapII 1292 CCGG C^CGG
HhaI 1437 GCGC GCG^C
HinP1I 1437 GCGC G^CGC
HincII 7 GTTGAC GT[CT]^[GA]AC
HindII 7 GTTGAC GT[CT]^[GA]AC
HpaII 1292 CCGG C^CGG
MamI 387 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1520 TTAA T^TAA
MslI 826 CATGTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1292 CCGG C^CGG
NcoI 1205 CCATGG C^CATGG
NspI 927 GCATGC [GA]CATG^[CT]
PaeR7I 1596 CTCGAG C^TCGAG
PleI 58 GACTC GAGTC(4/5)
PstI 1227 CTGCAG CTGCA^G
RcaI 165 TCATGA T^CATGA
SapI 802 GAAGAGC GCTCTTC(1/4)
SbfI 1226 CCTGCAGG CCTGCA^GG
SexAI 146 ACCAGGT A^CC[AT]GGT
SphI 927 GCATGC GCATG^C
Sse8387I 1226 CCTGCAGG CCTGCA^GG
StuI 27 AGGCCT AGG^CCT
TaqI 1597 TCGA T^CGA
Tru9I 1520 TTAA T^TAA
TthHB8I 1597 TCGA T^CGA
XcmI 147 CCAGGTGATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1596 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 18 enzymes) #
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Asp700I 1494 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1578 GAAAAGTTTC
BseRI 1079 CTCCTC GAGGAG(10/8)
1162 GAGGAG
BspMI 853 ACCTGC ACCTGC(4/8)
1225 ACCTGC
DraIII 538 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
1149 CACCATGTG
EcoRI 504 GAATTC G^AATTC
1257 GAATTC
EcoT14I 24 CCTAGG C^C[AT][AT]GG
1205 CCATGG
HindIII 351 AAGCTT A^AGCTT
941 AAGCTT
HphI 150 GGTGA GGTGA(8/7)
1275 TCACC
MspA1I 1072 CAGCTG C[AC]G^C[GT]G
1587 CAGCTG
PflMI 316 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1148 CCACCATGTGG
PpuMI 362 GGGACCC [GA]G^G[AT]CC[CT]
629 AGGACCT
PvuII 1072 CAGCTG CAG^CTG
1587 CAGCTG
SacI 919 GAGCTC GAGCT^C
1593 GAGCTC
SfcI 208 CTACAG C^T[GA][CT]AG
1227 CTGCAG
StyI 24 CCTAGG C^C[AT][AT]GG
1205 CCATGG
Tsp45I 337 GTGAC ^GT[GC]AC
1421 GTGAC
Van91I 316 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1148 CCACCATGTGG
XmnI 1494 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1578 GAAAAGTTTC
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
AocI ApoI AspHI AvaII BanII
BbvI BfaI BmyI BpmI BsaI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraII EarI Eco81I
EcoNI EcoO109I EcoRII Fnu4HI FokI
HaeIII HinfI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI SmlI
TfiI TseI Tsp509I TspRI XhoII