Restriction map for
pFN21AB7853
(Linear) (Six-base) MAPSORT of: KIBB7853.seq Check: 1590 from: 1 to: 1647
KIBB7853.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 862 1234 1647
Size: 862 372 413
Fragments arranged by size:
862 413 372
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 1647
Size: 929 718
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 49 81 1647
Size: 49 32 1566
Fragments arranged by size:
1566 49 32
AlwNI CAG_nnn'CTG
Cuts at: 0 82 267 375 520 1647
Size: 82 185 108 145 1127
Fragments arranged by size:
1127 185 145 108 82
ApaLI G'TGCA_C
Cuts at: 0 1311 1647
Size: 1311 336
ApoI r'AATT_y
Cuts at: 0 504 793 894 1257 1647
Size: 504 289 101 363 390
Fragments arranged by size:
504 390 363 289 101
AvaI C'yCGr_G
Cuts at: 0 1596 1647
Size: 1596 51
AvrII C'CTAG_G
Cuts at: 0 24 1647
Size: 24 1623
BanI G'GyrC_C
Cuts at: 0 480 1647
Size: 480 1167
BanII G_rGCy'C
Cuts at: 0 467 923 1203 1597 1647
Size: 467 456 280 394 50
Fragments arranged by size:
467 456 394 280 50
BbsI GAAGACnn'nnnn_
Cuts at: 0 1249 1647
Size: 1249 398
BciVI GTATCCnnnnn_n'
Cuts at: 0 403 1647
Size: 403 1244
BclI T'GATC_A
Cuts at: 0 617 1647
Size: 617 1030
BglI GCCn_nnn'nGGC
Cuts at: 0 1207 1647
Size: 1207 440
Bme1580I G_kGCm'C
Cuts at: 0 1315 1647
Size: 1315 332
BmrI ACTGGGnnnn_n'
Cuts at: 0 57 717 1647
Size: 57 660 930
Fragments arranged by size:
930 660 57
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 117 1042 1647
Size: 117 925 605
Fragments arranged by size:
925 605 117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 625 742 1565 1611 1647
Size: 87 538 117 823 46 36
Fragments arranged by size:
823 538 117 87 46 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 993 1067 1180 1647
Size: 520 473 74 113 467
Fragments arranged by size:
520 473 467 113 74
BsaI GGTCTCn'nnnn_
Cuts at: 0 106 163 617 718 747 1168 1647
Size: 106 57 454 101 29 421 479
Fragments arranged by size:
479 454 421 106 101 57 29
BsaBI GATnn'nnATC
Cuts at: 0 391 1647
Size: 391 1256
BsaWI w'CCGG_w
Cuts at: 0 1291 1647
Size: 1291 356
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1070 1177 1647
Size: 1070 107 470
Fragments arranged by size:
1070 470 107
BseYI C'CCAG_C
Cuts at: 0 590 651 1647
Size: 590 61 996
Fragments arranged by size:
996 590 61
BsiHKAI G_wGCw'C
Cuts at: 0 923 1315 1597 1647
Size: 923 392 282 50
Fragments arranged by size:
923 392 282 50
Bsp1286I G_dGCh'C
Cuts at: 0 467 923 1203 1315 1597 1647
Size: 467 456 280 112 282 50
Fragments arranged by size:
467 456 282 280 112 50
BspHI T'CATG_A
Cuts at: 0 165 1647
Size: 165 1482
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 862 1234 1647
Size: 862 372 413
Fragments arranged by size:
862 413 372
BsrDI GCAATG_nn'
Cuts at: 0 388 1647
Size: 388 1259
BssSI C'ACGA_G
Cuts at: 0 1444 1647
Size: 1444 203
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1319 1647
Size: 1319 328
BstYI r'GATC_y
Cuts at: 0 236 245 287 711 839 1618 1647
Size: 236 9 42 424 128 779 29
Fragments arranged by size:
779 424 236 128 42 29 9
Bsu36I CC'TnA_GG
Cuts at: 0 485 513 662 1031 1647
Size: 485 28 149 369 616
Fragments arranged by size:
616 485 369 149 28
BtgI C'CryG_G
Cuts at: 0 1205 1647
Size: 1205 442
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 23 1647
Size: 23 1624
BtsI GCAGTG_nn'
Cuts at: 0 341 643 1647
Size: 341 302 1004
Fragments arranged by size:
1004 341 302
DraIII CAC_nnn'GTG
Cuts at: 0 543 1154 1647
Size: 543 611 493
Fragments arranged by size:
611 543 493
EarI CTCTTCn'nnn_
Cuts at: 0 323 797 1162 1647
Size: 323 474 365 485
Fragments arranged by size:
485 474 365 323
EcoICRI GAG'CTC
Cuts at: 0 921 1595 1647
Size: 921 674 52
Fragments arranged by size:
921 674 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 87 625 742 929 1565 1611 1647
Size: 87 538 117 187 636 46 36
Fragments arranged by size:
636 538 187 117 87 46 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 91 1133 1251 1647
Size: 91 1042 118 396
Fragments arranged by size:
1042 396 118 91
EcoO109I rG'GnC_Cy
Cuts at: 0 21 363 517 630 1647
Size: 21 342 154 113 1017
Fragments arranged by size:
1017 342 154 113 21
EcoRI G'AATT_C
Cuts at: 0 504 1257 1647
Size: 504 753 390
Fragments arranged by size:
753 504 390
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 398 403 420 430 435 452 493 525
Size: 398 5 17 10 5 17 41 32
Cuts at: 525 1647
Size: 1122
Fragments arranged by size:
1122 398 41 32 17 17 10 5
5
HincII GTy'rAC
Cuts at: 0 9 1647
Size: 9 1638
HindIII A'AGCT_T
Cuts at: 0 351 941 1647
Size: 351 590 706
Fragments arranged by size:
706 590 351
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 1647
Size: 236 1411
MslI CAynn'nnrTG
Cuts at: 0 830 1647
Size: 830 817
MspA1I CmG'CkG
Cuts at: 0 1074 1589 1647
Size: 1074 515 58
Fragments arranged by size:
1074 515 58
NcoI C'CATG_G
Cuts at: 0 1205 1647
Size: 1205 442
NspI r_CATG'y
Cuts at: 0 931 1647
Size: 931 716
PflMI CCAn_nnn'nTGG
Cuts at: 0 322 1154 1647
Size: 322 832 493
Fragments arranged by size:
832 493 322
PfoI T'CCnGG_A
Cuts at: 0 315 1647
Size: 315 1332
PpuMI rG'GwC_Cy
Cuts at: 0 363 630 1647
Size: 363 267 1017
Fragments arranged by size:
1017 363 267
PsiI TTA'TAA
Cuts at: 0 1559 1647
Size: 1559 88
PspXI vC'TCGA_Gb
Cuts at: 0 1596 1647
Size: 1596 51
PstI C_TGCA'G
Cuts at: 0 1231 1647
Size: 1231 416
PvuII CAG'CTG
Cuts at: 0 1074 1589 1647
Size: 1074 515 58
Fragments arranged by size:
1074 515 58
SacI G_AGCT'C
Cuts at: 0 923 1597 1647
Size: 923 674 50
Fragments arranged by size:
923 674 50
SanDI GG'GwC_CC
Cuts at: 0 363 1647
Size: 363 1284
SapI GCTCTTCn'nnn_
Cuts at: 0 797 1647
Size: 797 850
SbfI CC_TGCA'GG
Cuts at: 0 1231 1647
Size: 1231 416
SexAI A'CCwGG_T
Cuts at: 0 146 1647
Size: 146 1501
SfcI C'TryA_G
Cuts at: 0 208 1227 1647
Size: 208 1019 420
Fragments arranged by size:
1019 420 208
SmlI C'TyrA_G
Cuts at: 0 499 972 1082 1159 1596 1647
Size: 499 473 110 77 437 51
Fragments arranged by size:
499 473 437 110 77 51
SphI G_CATG'C
Cuts at: 0 931 1647
Size: 931 716
StuI AGG'CCT
Cuts at: 0 29 1647
Size: 29 1618
StyI C'CwwG_G
Cuts at: 0 24 1205 1647
Size: 24 1181 442
Fragments arranged by size:
1181 442 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 288 1647
Size: 288 1359
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 154 1647
Size: 154 1493
XhoI C'TCGA_G
Cuts at: 0 1596 1647
Size: 1596 51
XmnI GAAnn'nnTTC
Cuts at: 0 1498 1582 1647
Size: 1498 84 65
Fragments arranged by size:
1498 84 65
Enzymes that do cut:
AarI AcuI AloI AlwNI ApaLI ApoI AvaI AvrII
BanI BanII BbsI BciVI BclI BglI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaBI BsaWI BseRI BseYI
BsiHKAI Bsp1286I BspHI BspMI BsrDI BssSI BstAPI BstYI
Bsu36I BtgI BtgZI BtsI DraIII EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI Hin4I HincII HindIII MmeI MslI
MspA1I NcoI NspI PflMI PfoI PpuMI PsiI PspXI
PstI PvuII SacI SanDI SapI SbfI SexAI SfcI
SmlI SphI StuI StyI TaqII XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI ApaI AscI AseI AsiSI BaeI
BamHI BbeI BbvCI BcgI BfrBI BglII BlpI BmgBI
BmtI Bpu10I BsaAI BsaHI BsaXI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BstBI
BstEII BstXI BstZ17I ClaI CspCI DraI DrdI EaeI
EagI EciI EcoRV FalI FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PpiI PshAI PspOMI PsrI PvuI
RsrII SacII SalI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI TatI TstI
Tth111I XbaI XmaI ZraI