Restriction map for
pFN21AE1126
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbsI BclI BfrI BglII BlnI
BpuAI BsaBI BsaI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrDI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII Eam1105I Eco47III EcoO65I
EcoRI EcoRV EcoT22I FbaI FspI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MluI MseI
MunI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI ScaI SexAI
SfuI SgrAI SmlI SnaBI SpeI
SphI SspBI SspI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AfaI 1218 GTAC GT^AC
AflIII 385 ACGTGT A^C[GA][CT]GT
AscI 1232 GGCGCGCC GG^CGCGCC
Asp700I 543 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 886 CACGTG CAC^GTG
BbvCI 828 CCTCAGC CCTCAGC(-5/-2)
BcgI 1057 CGAGCCCGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 852 GGATAC GTATCC(6/5)
BsaAI 886 CACGTG [CT]AC^GT[GA]
BsrFI 605 GCCGGC [GA]^CCGG[CT]
BssHII 1233 GCGCGC G^CGCGC
BstYI 824 AGATCC [GA]^GATC[CT]
Cfr10I 605 GCCGGC [GA]^CCGG[CT]
DrdI 496 GACACCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 604 GGCCGGCC GGCCGG^CC
HgaI 1256 GACGC GACGC(5/10)
MflI 824 AGATCC [GA]^GATC[CT]
NaeI 605 GCCGGC GCC^GGC
NdeI 672 CATATG CA^TATG
NgoMIV 605 GCCGGC G^CCGGC
NheI 1278 GCTAGC G^CTAGC
NotI 357 GCGGCCGC GC^GGCCGC
NspI 987 GCATGT [GA]CATG^[CT]
PmaCI 886 CACGTG CAC^GTG
PmlI 886 CACGTG CAC^GTG
PpuMI 1460 GGGTCCT [GA]G^G[AT]CC[CT]
RsaI 1218 GTAC GT^AC
SbfI 630 CCTGCAGG CCTGCA^GG
SfiI 1767 GGCCACAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 630 CCTGCAGG CCTGCA^GG
TaqI 1403 TCGA T^CGA
TthHB8I 1403 TCGA T^CGA
XhoII 824 AGATCC [GA]^GATC[CT]
XmnI 543 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 193 CGCG CG^CG
1234 CGCG
AccIII 320 TCCGGA T^CCGGA
1341 TCCGGA
AlwI 825 GATCC GGATC(4/5)
782 GGATC
AocI 447 CCTTAGG CC^T[ACGT]AGG
1371 CCTGAGG
ApaI 133 GGGCCC GGGCC^C
1324 GGGCCC
AvaI 152 CCCGGG C^[CT]CG[GA]G
258 CCCGGG
BbeI 147 GGCGCC GGCGC^C
649 GGCGCC
BsaWI 320 TCCGGA [AT]^CCGG[AT]
1341 TCCGGA
BseAI 320 TCCGGA T^CCGGA
1341 TCCGGA
BsiEI 358 CGGCCG CG[GA][CT]^CG
603 CGGCCG
BsoBI 152 CCCGGG C^[CT]CG[GA]G
258 CCCGGG
BspEI 320 TCCGGA T^CCGGA
1341 TCCGGA
BspMI 166 ACCTGC ACCTGC(4/8)
641 GCAGGT
BstUI 193 CGCG CG^CG
1234 CGCG
BstXI 173 CCATCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
200 CCATCTCGCTGG
Bsu36I 447 CCTTAGG CC^T[ACGT]AGG
1371 CCTGAGG
EagI 358 CGGCCG C^GGCCG
603 CGGCCG
EarI 309 GAAGAG CTCTTC(1/4)
1888 GAAGAG
EclXI 358 CGGCCG C^GGCCG
603 CGGCCG
Eco52I 358 CGGCCG C^GGCCG
603 CGGCCG
Eco81I 447 CCTTAGG CC^T[ACGT]AGG
1371 CCTGAGG
HaeII 147 GGCGCC [GA]GCGC^[CT]
649 GGCGCC
KasI 147 GGCGCC G^GCGCC
649 GGCGCC
Ksp632I 309 GAAGAG CTCTTC(1/4)
1888 GAAGAG
MroI 320 TCCGGA T^CCGGA
1341 TCCGGA
MslI 672 CATATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
801 CACCACGATG
MvnI 193 CGCG CG^CG
1234 CGCG
NarI 147 GGCGCC GG^CGCC
649 GGCGCC
NcoI 143 CCATGG C^CATGG
1521 CCATGG
PvuII 1360 CAGCTG CAG^CTG
1715 CAGCTG
SacI 157 GAGCTC GAGCT^C
813 GAGCTC
SfoI 147 GGCGCC GGC^GCC
649 GGCGCC
SmaI 152 CCCGGG CCC^GGG
258 CCCGGG
StuI 126 AGGCCT AGG^CCT
1598 AGGCCT
XmaCI 152 CCCGGG C^CCGGG
258 CCCGGG
XmaI 152 CCCGGG C^CCGGG
258 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcyI AluI AlwNI AspHI
AvaII BalI BanI BanII BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TseI Tsp45I
TspRI Van91I XcmI