Restriction map for
pFN21AB7633
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AvaI AviII AvrII
BamHI BanI BbeI BbvCI BcgI
BciVI BcnI BfaI BglI BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI BsmFI BsoBI Bsp1407I BspEI
BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Cac8I CelII CfoI Cfr10I CpoI
DraI DraII DraIII DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FauI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI Ksp632I KspI MaeI MamI
MluI MroI MspI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PleI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AfaI 841 GTAC GT^AC
AlwI 198 GGATC GGATC(4/5)
AocI 834 CCTGAGG CC^T[ACGT]AGG
AspI 176 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 93 GGACC G^G[AT]CC
BalI 945 TGGCCA TGG^CCA
BanII 489 GAGCTC G[GA]GC[CT]^C
BbrPI 844 CACGTG CAC^GTG
BbvI 4 GCTGC GCAGC(8/12)
BclI 462 TGATCA T^GATCA
BglII 430 AGATCT A^GATCT
BsaAI 844 CACGTG [CT]AC^GT[GA]
BseRI 76 GAGGAG GAGGAG(10/8)
BsgI 248 GTGCAG GTGCAG(16/14)
BspDI 652 ATCGAT AT^CGAT
BspLU11I 591 ACATGT A^CATGT
BsrDI 86 CATTGC GCAATG(2/0)
BstYI 430 AGATCT [GA]^GATC[CT]
Bsu36I 834 CCTGAGG CC^T[ACGT]AGG
Cfr13I 93 GGACC G^G[ACGT]CC
ClaI 652 ATCGAT AT^CGAT
DrdI 18 GACAACCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 945 TGGCCA [CT]^GGCC[GA]
Eco81I 834 CCTGAGG CC^T[ACGT]AGG
EcoRI 822 GAATTC G^AATTC
EcoT14I 191 CCAAGG C^C[AT][AT]GG
FbaI 462 TGATCA T^GATCA
Fnu4HI 4 GCTGC GC^[ACGT]GC
HinfI 935 GAATC G^A[ACGT]TC
ItaI 4 GCTGC GC^[ACGT]GC
MaeII 845 ACGT A^CGT
MfeI 284 CAATTG C^AATTG
MflI 430 AGATCT [GA]^GATC[CT]
MluNI 945 TGGCCA TGG^CCA
MscI 945 TGGCCA TGG^CCA
MspA1I 208 CAGCTG C[AC]G^C[GT]G
MunI 284 CAATTG C^AATTG
NspI 591 ACATGT [GA]CATG^[CT]
PflFI 176 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 48 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 844 CACGTG CAC^GTG
PmlI 844 CACGTG CAC^GTG
PvuII 208 CAGCTG CAG^CTG
RsaI 841 GTAC GT^AC
SacI 489 GAGCTC GAGCT^C
Sau96I 93 GGACC G^G[ACGT]CC
SexAI 22 ACCTGGT A^CC[AT]GGT
StuI 812 AGGCCT AGG^CCT
StyI 191 CCAAGG C^C[AT][AT]GG
TaqI 653 TCGA T^CGA
TfiI 935 GAATC G^A[AT]TC
TseI 4 GCTGC G^C[AT]GC
Tth111I 176 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 653 TCGA T^CGA
Van91I 48 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 430 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 230 GAATTT [GA]^AATT[CT]
822 GAATTC
AflII 415 CTTAAG C^TTAAG
667 CTTAAG
AflIII 591 ACATGT A^C[GA][CT]GT
843 ACACGT
Alw44I 505 GTGCAC G^TGCAC
757 GTGCAC
AlwNI 378 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
630 CAGTGACTG
ApaLI 505 GTGCAC G^TGCAC
757 GTGCAC
ApoI 230 GAATTT [GA]^AATT[CT]
822 GAATTC
Asp700I 390 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
558 GAAGGCTTTC
BbsI 213 GAAGAC GAAGAC(2/6)
408 GTCTTC
BfrI 415 CTTAAG C^TTAAG
667 CTTAAG
BpuAI 213 GAAGAC GAAGAC(2/6)
408 GTCTTC
BsiYI 48 CCAGGAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
152 CCTCAGTAGGG
BslI 48 CCAGGAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
152 CCTCAGTAGGG
BsmI 373 GAATGC GAATGC(1/-1)
625 GAATGC
HphI 241 GGTGA GGTGA(8/7)
778 GGTGA
MboII 213 GAAGA GAAGA(8/7)
409 TCTTC
MslI 496 CATCTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
772 CACACTGGTG
NlaIV 93 GGACCC GG[ACGT]^[ACGT]CC
143 GGAACC
PstI 5 CTGCAG CTGCA^G
171 CTGCAG
SfaNI 126 GATGC GCATC(5/9)
363 GATGC
SfcI 5 CTGCAG C^T[GA][CT]AG
171 CTGCAG
SmlI 415 CTTAAG C^T[CT][GA]AG
667 CTTAAG
XmnI 390 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
558 GAAGGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AluI AspHI BmyI BpmI BsaJI
BsiHKAI Bsp1286I BsrI BssKI BstF5I
BstNI DdeI DpnII EcoRII FokI
HaeIII MaeIII MboI MnlI MseI
MvaI NdeII NlaIII Sau3AI ScrFI
Tru9I Tsp45I Tsp509I TspRI