Restriction map for
pFN21AB7633
(Linear) (Six-base) MAPSORT of: KIBB7633.seq Check: 6525 from: 1 to: 963
KIBB7633.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 568 717 963
Size: 232 336 149 246
Fragments arranged by size:
336 246 232 149
AflII C'TTAA_G
Cuts at: 0 415 667 963
Size: 415 252 296
Fragments arranged by size:
415 296 252
AflIII A'CryG_T
Cuts at: 0 591 843 963
Size: 591 252 120
Fragments arranged by size:
591 252 120
AleI CACnn'nnGTG
Cuts at: 0 776 963
Size: 776 187
AlwNI CAG_nnn'CTG
Cuts at: 0 383 635 963
Size: 383 252 328
Fragments arranged by size:
383 328 252
ApaLI G'TGCA_C
Cuts at: 0 505 757 963
Size: 505 252 206
Fragments arranged by size:
505 252 206
ApoI r'AATT_y
Cuts at: 0 230 822 963
Size: 230 592 141
Fragments arranged by size:
592 230 141
BanII G_rGCy'C
Cuts at: 0 493 963
Size: 493 470
BbsI GAAGACnn'nnnn_
Cuts at: 0 220 401 963
Size: 220 181 562
Fragments arranged by size:
562 220 181
BclI T'GATC_A
Cuts at: 0 462 963
Size: 462 501
BglII A'GATC_T
Cuts at: 0 430 963
Size: 430 533
Bme1580I G_kGCm'C
Cuts at: 0 509 761 963
Size: 509 252 202
Fragments arranged by size:
509 252 202
BmrI ACTGGGnnnn_n'
Cuts at: 0 264 963
Size: 264 699
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 377 461 545 629 713 881 963
Size: 151 226 84 84 84 84 168 82
Fragments arranged by size:
226 168 151 84 84 84 84 82
BsaAI yAC'GTr
Cuts at: 0 846 963
Size: 846 117
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 963
Size: 91 872
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 963
Size: 269 694
BsiHKAI G_wGCw'C
Cuts at: 0 493 509 761 963
Size: 493 16 252 202
Fragments arranged by size:
493 252 202 16
BsmI GAATG_Cn'
Cuts at: 0 379 631 963
Size: 379 252 332
Fragments arranged by size:
379 332 252
Bsp1286I G_dGCh'C
Cuts at: 0 493 509 761 963
Size: 493 16 252 202
Fragments arranged by size:
493 252 202 16
BsrDI GCAATG_nn'
Cuts at: 0 85 963
Size: 85 878
BstYI r'GATC_y
Cuts at: 0 430 963
Size: 430 533
Bsu36I CC'TnA_GG
Cuts at: 0 835 963
Size: 835 128
BtsI GCAGTG_nn'
Cuts at: 0 65 384 636 963
Size: 65 319 252 327
Fragments arranged by size:
327 319 252 65
ClaI AT'CG_AT
Cuts at: 0 653 963
Size: 653 310
DrdI GACnn_nn'nnGTC
Cuts at: 0 24 963
Size: 24 939
EaeI y'GGCC_r
Cuts at: 0 945 963
Size: 945 18
EcoICRI GAG'CTC
Cuts at: 0 491 963
Size: 491 472
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 232 377 461 545 568 629 713
Size: 151 81 145 84 84 23 61 84
Cuts at: 713 717 881 963
Size: 4 164 82
Fragments arranged by size:
164 151 145 84 84 84 82 81
61 23 4
EcoRI G'AATT_C
Cuts at: 0 822 963
Size: 822 141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 480 512 646 678 963
Size: 480 32 134 32 285
Fragments arranged by size:
480 285 134 32 32
MfeI C'AATT_G
Cuts at: 0 284 963
Size: 284 679
MscI TGG'CCA
Cuts at: 0 947 963
Size: 947 16
MslI CAynn'nnrTG
Cuts at: 0 500 776 963
Size: 500 276 187
Fragments arranged by size:
500 276 187
MspA1I CmG'CkG
Cuts at: 0 210 963
Size: 210 753
NspI r_CATG'y
Cuts at: 0 595 963
Size: 595 368
PciI A'CATG_T
Cuts at: 0 591 963
Size: 591 372
PflMI CCAn_nnn'nTGG
Cuts at: 0 54 963
Size: 54 909
PfoI T'CCnGG_A
Cuts at: 0 47 963
Size: 47 916
PmlI CAC'GTG
Cuts at: 0 846 963
Size: 846 117
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 136 168 963
Size: 136 32 795
Fragments arranged by size:
795 136 32
PsiI TTA'TAA
Cuts at: 0 875 922 963
Size: 875 47 41
Fragments arranged by size:
875 47 41
PstI C_TGCA'G
Cuts at: 0 9 175 963
Size: 9 166 788
Fragments arranged by size:
788 166 9
PvuII CAG'CTG
Cuts at: 0 210 963
Size: 210 753
SacI G_AGCT'C
Cuts at: 0 493 963
Size: 493 470
SexAI A'CCwGG_T
Cuts at: 0 22 963
Size: 22 941
SfcI C'TryA_G
Cuts at: 0 5 171 963
Size: 5 166 792
Fragments arranged by size:
792 166 5
SmlI C'TyrA_G
Cuts at: 0 415 667 963
Size: 415 252 296
Fragments arranged by size:
415 296 252
StuI AGG'CCT
Cuts at: 0 814 963
Size: 814 149
StyI C'CwwG_G
Cuts at: 0 191 963
Size: 191 772
Tth111I GACn'n_nGTC
Cuts at: 0 179 963
Size: 179 784
XmnI GAAnn'nnTTC
Cuts at: 0 394 562 963
Size: 394 168 401
Fragments arranged by size:
401 394 168
Enzymes that do cut:
AcuI AflII AflIII AleI AlwNI ApaLI ApoI BanII
BbsI BclI BglII Bme1580I BmrI BpmI BsaAI BseRI
BsgI BsiHKAI BsmI Bsp1286I BsrDI BstYI Bsu36I BtsI
ClaI DrdI EaeI EcoICRI Eco57MI EcoRI Hin4I MfeI
MscI MslI MspA1I NspI PciI PflMI PfoI PmlI
PpiI PsiI PstI PvuII SacI SexAI SfcI SmlI
StuI StyI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AlfI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BfrBI
BglI BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiWI BsmBI
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI CspCI
DraI DraIII EagI EarI EciI EcoNI EcoO109I EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PmeI
PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TatI TstI XbaI XcmI XhoI XmaI ZraI