(Circular) (Six-base) MAPSORT of: pFN21AB7837.seq Check: 8176 from: 1 to: 6325 pFN21AB7837 6325 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4135 278 Size: 53 83 186 936 2599 2468 Fragments arranged by size: 2599 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3668 1937 Size: 1731 4594 Acc65I G'GTAC_C Cuts at: 3646 3646 Size: 6325 AclI AA'CG_TT Cuts at: 1563 4452 4825 1563 Size: 2889 373 3063 Fragments arranged by size: 3063 2889 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2434 2854 2938 3358 3606 4388 5772 6040 Size: 802 420 84 420 248 782 1384 268 Cuts at: 6040 1632 Size: 1917 Fragments arranged by size: 1917 1384 802 782 420 420 268 248 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6128 AflIII A'CryG_T Cuts at: 1203 2426 3570 5224 6201 1203 Size: 1223 1144 1654 977 1327 Fragments arranged by size: 1654 1327 1223 1144 977 AgeI A'CCGG_T Cuts at: 5156 5156 Size: 6325 AhdI GACnn_n'nnGTC Cuts at: 5054 5054 Size: 6325 AleI CACnn'nnGTG Cuts at: 1166 2997 3417 1166 Size: 1831 420 4074 Fragments arranged by size: 4074 1831 420 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6325 AlwNI CAG_nnn'CTG Cuts at: 2278 4068 5640 2278 Size: 1790 1572 2963 Fragments arranged by size: 2963 1790 1572 ApaLI G'TGCA_C Cuts at: 4382 5538 4382 Size: 1156 5169 ApoI r'AATT_y Cuts at: 1493 2250 2820 2988 3634 3860 4012 6121 1493 Size: 757 570 168 646 226 152 2109 1697 Fragments arranged by size: 2109 1697 757 646 570 226 168 152 AseI AT'TA_AT Cuts at: 160 1032 4879 160 Size: 872 3847 1606 Fragments arranged by size: 3847 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6325 AvaI C'yCGr_G Cuts at: 1870 1943 3650 1870 Size: 73 1707 4545 Fragments arranged by size: 4545 1707 73 BamHI G'GATC_C Cuts at: 3655 4022 3655 Size: 367 5958 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3646 5101 618 Size: 325 217 627 1859 1455 1842 Fragments arranged by size: 1859 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3644 1391 Size: 2253 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2563 2731 3151 928 Size: 971 664 168 420 4102 Fragments arranged by size: 4102 971 664 420 168 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4516 4550 1903 Size: 34 2579 34 3678 Fragments arranged by size: 3678 2579 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3140 4217 5427 3140 Size: 1077 1210 4038 Fragments arranged by size: 4038 1210 1077 BclI T'GATC_A Cuts at: 1548 1548 Size: 6325 BfrBI ATG'CAT Cuts at: 3609 3609 Size: 6325 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4936 136 Size: 107 122 71 631 605 3264 1525 Fragments arranged by size: 3264 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3041 3461 6320 3041 Size: 420 2859 3046 Fragments arranged by size: 3046 2859 420 BlpI GC'TnA_GC Cuts at: 3742 3742 Size: 6325 Bme1580I G_kGCm'C Cuts at: 1790 4386 5542 1790 Size: 2596 1156 2573 Fragments arranged by size: 2596 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6325 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2622 4056 5014 Size: 416 53 83 399 24 1205 1434 958 Cuts at: 5014 442 Size: 1753 Fragments arranged by size: 1753 1434 1205 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6325 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2321 2519 2321 Size: 198 6127 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2316 2514 2682 2766 2850 2934 3102 3186 Size: 1077 198 168 84 84 84 168 84 Cuts at: 3186 3270 3354 4985 1239 Size: 84 84 1631 2579 Fragments arranged by size: 2579 1631 1077 198 168 168 84 84 84 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4450 5315 5613 4450 Size: 865 298 5162 Fragments arranged by size: 5162 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4988 882 Size: 617 3489 2219 Fragments arranged by size: 3489 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2429 4116 493 Size: 711 1225 1687 2702 Fragments arranged by size: 2702 1687 1225 711 BsaBI GATnn'nnATC Cuts at: 6319 6319 Size: 6325 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4132 4514 5149 275 Size: 53 83 186 936 2599 382 635 1451 Fragments arranged by size: 2599 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4758 5156 5430 5577 5988 4758 Size: 398 274 147 411 5095 Fragments arranged by size: 5095 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6100 Fragments arranged by size: 6100 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 1186 Size: 880 5445 BseYI C'CCAG_C Cuts at: 5528 6024 5528 Size: 496 5829 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5752 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3775 4536 5564 1540 Size: 275 186 1774 761 1028 2301 Fragments arranged by size: 2301 1774 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4386 4471 5542 4386 Size: 85 1071 5169 Fragments arranged by size: 5169 1071 85 BsmI GAATG_Cn' Cuts at: 2936 3119 3836 3929 2936 Size: 183 717 93 5332 Fragments arranged by size: 5332 717 183 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3644 4386 4471 5542 1391 Size: 399 1854 742 85 1071 2174 Fragments arranged by size: 2174 1854 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5988 5988 Size: 6325 BspHI T'CATG_A Cuts at: 4214 5944 4214 Size: 1730 4595 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3680 844 Size: 2836 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4212 1394 Size: 429 2389 3507 Fragments arranged by size: 3507 2389 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2861 3365 3517 4820 4994 65 Size: 1183 1613 504 152 1303 174 1396 Fragments arranged by size: 1613 1396 1303 1183 504 174 152 BsrFI r'CCGG_y Cuts at: 1700 1810 4969 5156 1700 Size: 110 3159 187 2869 Fragments arranged by size: 3159 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6325 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6325 BssSI C'ACGA_G Cuts at: 4385 5397 4385 Size: 1012 5313 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6325 BstBI TT'CG_AA Cuts at: 2470 2638 2722 3226 3310 3394 5129 2470 Size: 168 84 504 84 84 1735 3666 Fragments arranged by size: 3666 1735 504 168 84 84 84 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6325 BstYI r'GATC_y Cuts at: 1972 2068 3041 3461 3655 4022 4407 4424 5876 Size: 96 973 420 194 367 385 17 1452 Cuts at: 5876 5962 5974 6320 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 973 420 385 367 346 194 96 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4834 Fragments arranged by size: 4834 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3457 487 Size: 683 56 2231 3355 Fragments arranged by size: 3355 2231 683 56 BtsI GCAGTG_nn' Cuts at: 2945 3841 4635 4655 2945 Size: 896 794 20 4615 Fragments arranged by size: 4615 896 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6325 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2923 2958 3511 3546 529 Size: 35 2359 35 553 35 3308 Fragments arranged by size: 3308 2359 553 35 35 35 DraI TTT'AAA Cuts at: 2845 3629 2845 Size: 784 5541 DraIII CAC_nnn'GTG Cuts at: 2576 2576 Size: 6325 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5332 809 Size: 507 4016 1802 Fragments arranged by size: 4016 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3772 4661 6290 Size: 54 1425 50 275 18 1942 889 1629 Cuts at: 6290 8 Size: 43 Fragments arranged by size: 1942 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3772 1537 Size: 275 1960 4090 Fragments arranged by size: 4090 1960 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4255 1353 Size: 2902 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4912 5286 5432 387 Size: 1064 138 3323 374 146 1280 Fragments arranged by size: 3323 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2316 2434 2514 2682 2766 2850 2854 Size: 393 684 118 80 168 84 84 4 Cuts at: 2854 2934 2938 3102 3186 3270 3354 3358 3606 Size: 80 4 164 84 84 84 4 248 Cuts at: 3606 4388 4985 5772 6040 1239 Size: 782 597 787 268 1524 Fragments arranged by size: 1524 787 782 684 597 393 268 248 168 164 118 84 84 84 84 84 80 80 4 4 4 EcoO109I rG'GnC_Cy Cuts at: 3173 3173 Size: 6325 EcoRI G'AATT_C Cuts at: 2820 2988 3634 4012 2820 Size: 168 646 378 5133 Fragments arranged by size: 5133 646 378 168 FspI TGC'GCA Cuts at: 4831 4831 Size: 6325 HaeII r_GCGC'y Cuts at: 1678 5472 1678 Size: 3794 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2550 2582 4966 4998 5040 Size: 32 135 32 978 32 2384 32 42 Cuts at: 5040 5072 1373 Size: 32 2626 Fragments arranged by size: 2626 2384 978 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3576 3669 3915 669 Size: 988 281 1638 93 246 3079 Fragments arranged by size: 3079 1638 988 281 246 93 HindIII A'AGCT_T Cuts at: 748 2613 3285 748 Size: 1865 672 3788 Fragments arranged by size: 3788 1865 672 HpaI GTT'AAC Cuts at: 3576 3915 3576 Size: 339 5986 KpnI G_GTAC'C Cuts at: 3650 3650 Size: 6325 MfeI C'AATT_G Cuts at: 3924 3924 Size: 6325 MluI A'CGCG_T Cuts at: 6201 6201 Size: 6325 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2420 5439 5623 1305 Size: 1115 3019 184 2007 Fragments arranged by size: 3019 2007 1115 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4503 Fragments arranged by size: 4503 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2593 2997 3417 4283 4642 4801 518 Size: 648 1427 404 420 866 359 159 2042 Fragments arranged by size: 2042 1427 866 648 420 404 359 159 MspA1I CmG'CkG Cuts at: 1606 3741 4418 5566 5811 1606 Size: 2135 677 1148 245 2120 Fragments arranged by size: 2135 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4834 Fragments arranged by size: 4834 939 552 NdeI CA'TA_TG Cuts at: 387 2202 387 Size: 1815 4510 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6325 NotI GC'GGCC_GC Cuts at: 3772 3772 Size: 6325 NsiI A_TGCA'T Cuts at: 3611 3611 Size: 6325 NspI r_CATG'y Cuts at: 1132 1229 3574 3584 3613 3683 5228 1132 Size: 97 2345 10 29 70 1545 2229 Fragments arranged by size: 2345 2229 1545 97 70 29 10 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6325 PciI A'CATG_T Cuts at: 3570 5224 3570 Size: 1654 4671 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6325 PmeI GTTT'AAAC Cuts at: 3629 3629 Size: 6325 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4394 4426 5907 5939 4394 Size: 32 1481 32 4780 Fragments arranged by size: 4780 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3173 3173 Size: 6325 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6325 PsiI TTA'TAA Cuts at: 2760 3180 3603 3895 2760 Size: 420 423 292 5190 Fragments arranged by size: 5190 423 420 292 PstI C_TGCA'G Cuts at: 830 3677 830 Size: 2847 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6325 SalI G'TCGA_C Cuts at: 1936 3667 1936 Size: 1731 4594 SbfI CC_TGCA'GG Cuts at: 3677 3677 Size: 6325 ScaI AGT'ACT Cuts at: 4573 6285 4573 Size: 1712 4613 SfcI C'TryA_G Cuts at: 826 1045 2089 2130 3673 4808 5489 5680 826 Size: 219 1044 41 1543 1135 681 191 1471 Fragments arranged by size: 1543 1471 1135 1044 681 219 191 41 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6325 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6325 SmaI CCC'GGG Cuts at: 1872 3652 1872 Size: 1780 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4429 5330 5592 820 Size: 197 926 2486 901 262 1553 Fragments arranged by size: 2486 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4116 493 Size: 3623 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6325 SphI G_CATG'C Cuts at: 1132 3683 1132 Size: 2551 3774 SspI AAT'ATT Cuts at: 5 52 4249 5 Size: 47 4197 2081 Fragments arranged by size: 4197 2081 47 StuI AGG'CCT Cuts at: 1517 2951 1517 Size: 1434 4891 StyI C'CwwG_G Cuts at: 513 1065 2004 3764 513 Size: 552 939 1760 3074 Fragments arranged by size: 3074 1760 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2060 4352 4369 4522 4707 5152 325 Size: 1255 480 2292 17 153 185 445 1498 Fragments arranged by size: 2292 1498 1255 480 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4571 6283 96 Size: 274 80 33 51 1443 2594 1712 138 Fragments arranged by size: 2594 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6285 6317 6285 Size: 32 6293 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6325 XbaI T'CTAG_A Cuts at: 3661 3661 Size: 6325 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2119 2011 Size: 108 6217 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6325 XmaI C'CCGG_G Cuts at: 1870 3650 1870 Size: 1780 4545 XmnI GAAnn'nnTTC Cuts at: 2447 2820 2951 2988 3008 3371 3408 4454 2447 Size: 373 131 37 20 363 37 1046 4318 Fragments arranged by size: 4318 1046 373 363 131 37 37 20 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4133 276 Size: 53 83 186 936 2599 2468 Fragments arranged by size: 2599 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI