(Circular) (Six-base) MAPSORT of: pFN21AB7850.seq Check: 9192 from: 1 to: 6574 pFN21AB7850 6574 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4384 278 Size: 53 83 186 936 2848 2468 Fragments arranged by size: 2848 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2124 3917 1937 Size: 187 1793 4594 Fragments arranged by size: 4594 1793 187 Acc65I G'GTAC_C Cuts at: 3895 3895 Size: 6574 AclI AA'CG_TT Cuts at: 1563 4701 5074 1563 Size: 3138 373 3063 Fragments arranged by size: 3138 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2018 2034 2744 3440 3621 3633 3843 4637 Size: 386 16 710 696 181 12 210 794 Cuts at: 4637 6021 6289 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 794 710 696 386 268 210 181 16 12 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6377 AflIII A'CryG_T Cuts at: 1203 2770 3380 3382 3619 5473 6450 1203 Size: 1567 610 2 237 1854 977 1327 Fragments arranged by size: 1854 1567 1327 977 610 237 2 AgeI A'CCGG_T Cuts at: 2097 5405 2097 Size: 3308 3266 AhdI GACnn_n'nnGTC Cuts at: 5303 5303 Size: 6574 AleI CACnn'nnGTG Cuts at: 1166 3543 1166 Size: 2377 4197 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6574 AlwNI CAG_nnn'CTG Cuts at: 2498 3755 4317 5889 2498 Size: 1257 562 1572 3183 Fragments arranged by size: 3183 1572 1257 562 ApaLI G'TGCA_C Cuts at: 3527 4631 5787 3527 Size: 1104 1156 4314 Fragments arranged by size: 4314 1156 1104 ApoI r'AATT_y Cuts at: 1493 2186 2982 3591 3883 4109 4261 6370 1493 Size: 693 796 609 292 226 152 2109 1697 Fragments arranged by size: 2109 1697 796 693 609 292 226 152 AseI AT'TA_AT Cuts at: 160 1032 2285 5128 160 Size: 872 1253 2843 1606 Fragments arranged by size: 2843 1606 1253 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6574 AvaI C'yCGr_G Cuts at: 1870 1943 3899 1870 Size: 73 1956 4545 Fragments arranged by size: 4545 1956 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3600 3633 3600 Size: 33 6541 BamHI G'GATC_C Cuts at: 3904 4271 3904 Size: 367 6207 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2528 3895 5350 618 Size: 325 217 627 741 1367 1455 1842 Fragments arranged by size: 1842 1455 1367 741 627 325 217 BanII G_rGCy'C Cuts at: 1391 3893 1391 Size: 2502 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5603 BbvCI CC'TCA_GC Cuts at: 3759 3789 3759 Size: 30 6544 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4765 4799 1903 Size: 34 2828 34 3678 Fragments arranged by size: 3678 2828 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4466 5676 4466 Size: 1210 5364 BclI T'GATC_A Cuts at: 1548 1548 Size: 6574 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5185 136 Size: 107 122 71 631 605 337 3176 1525 Fragments arranged by size: 3176 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3505 6569 3505 Size: 3064 3510 BlpI GC'TnA_GC Cuts at: 3991 3991 Size: 6574 Bme1580I G_kGCm'C Cuts at: 1790 3531 4635 5791 1790 Size: 1741 1104 1156 2573 Fragments arranged by size: 2573 1741 1156 1104 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6574 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2982 4305 5263 Size: 416 53 83 399 24 1565 1323 958 Cuts at: 5263 442 Size: 1753 Fragments arranged by size: 1753 1565 1323 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6574 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3165 3270 3497 5234 1239 Size: 1926 105 227 1737 2579 Fragments arranged by size: 2579 1926 1737 227 105 Bpu10I CC'TnA_GC Cuts at: 3759 3789 3759 Size: 30 6544 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4699 5564 5862 4699 Size: 865 298 5411 Fragments arranged by size: 5411 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3794 5237 882 Size: 617 2295 1443 2219 Fragments arranged by size: 2295 2219 1443 617 BsaAI yAC'GTr Cuts at: 493 1204 3383 3703 4365 493 Size: 711 2179 320 662 2702 Fragments arranged by size: 2702 2179 711 662 320 BsaBI GATnn'nnATC Cuts at: 6568 6568 Size: 6574 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4381 4763 5398 275 Size: 53 83 186 936 2848 382 635 1451 Fragments arranged by size: 2848 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2097 5007 5405 5679 5826 6237 2097 Size: 2910 398 274 147 411 2434 Fragments arranged by size: 2910 2434 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3722 3752 1178 Size: 30 165 30 2319 30 4000 Fragments arranged by size: 4000 2319 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3641 3743 1186 Size: 2455 102 4017 Fragments arranged by size: 4017 2455 102 BseYI C'CCAG_C Cuts at: 3213 5777 6273 3213 Size: 2564 496 3514 Fragments arranged by size: 3514 2564 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6001 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2071 4024 4785 5813 1540 Size: 275 186 70 1953 761 1028 2301 Fragments arranged by size: 2301 1953 1028 761 275 186 70 BsiHKAI G_wGCw'C Cuts at: 2612 2841 3531 4635 4720 5791 2612 Size: 229 690 1104 85 1071 3395 Fragments arranged by size: 3395 1104 1071 690 229 85 BsmI GAATG_Cn' Cuts at: 2033 3188 4085 4178 2033 Size: 1155 897 93 4429 Fragments arranged by size: 4429 1155 897 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2012 2012 Size: 6574 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2612 2841 3531 3893 4635 4720 5791 Size: 399 822 229 690 362 742 85 1071 Cuts at: 5791 1391 Size: 2174 Fragments arranged by size: 2174 1071 822 742 690 399 362 229 85 BspEI T'CCGG_A Cuts at: 6237 6237 Size: 6574 BspHI T'CATG_A Cuts at: 3088 3717 4463 6193 3088 Size: 629 746 1730 3469 Fragments arranged by size: 3469 1730 746 629 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3550 3929 844 Size: 2706 379 3489 Fragments arranged by size: 3489 2706 379 BsrBI CCG'CTC Cuts at: 1394 1823 4461 1394 Size: 429 2638 3507 Fragments arranged by size: 3507 2638 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2474 3449 5069 5243 65 Size: 1183 1226 975 1620 174 1396 Fragments arranged by size: 1620 1396 1226 1183 975 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2097 5218 5405 1700 Size: 110 287 3121 187 2869 Fragments arranged by size: 3121 2869 287 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6574 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6574 BssSI C'ACGA_G Cuts at: 2722 4634 5646 2722 Size: 1912 1012 3650 Fragments arranged by size: 3650 1912 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3755 794 Size: 2961 3613 BstBI TT'CG_AA Cuts at: 3046 5378 3046 Size: 2332 4242 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6574 BstXI CCAn_nnnn'nTGG Cuts at: 2539 2539 Size: 6574 BstYI r'GATC_y Cuts at: 1972 3505 3904 4271 4656 4673 6125 6211 6223 Size: 1533 399 367 385 17 1452 86 12 Cuts at: 6223 6569 1972 Size: 346 1977 Fragments arranged by size: 1977 1533 1452 399 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5083 Fragments arranged by size: 5083 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5835 Fragments arranged by size: 5835 683 56 BtsI GCAGTG_nn' Cuts at: 3129 4090 4884 4904 3129 Size: 961 794 20 4799 Fragments arranged by size: 4799 961 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6574 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6539 DraI TTT'AAA Cuts at: 2815 3034 3878 2815 Size: 219 844 5511 Fragments arranged by size: 5511 844 219 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5581 809 Size: 507 4265 1802 Fragments arranged by size: 4265 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4021 4910 6539 Size: 54 1425 50 275 18 2191 889 1629 Cuts at: 6539 8 Size: 43 Fragments arranged by size: 2191 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4021 1537 Size: 275 2209 4090 Fragments arranged by size: 4090 2209 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2010 4504 1353 Size: 657 2494 3423 Fragments arranged by size: 3423 2494 657 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5161 5535 5681 387 Size: 1064 138 3572 374 146 1280 Fragments arranged by size: 3572 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2018 2034 2744 3165 3270 3440 3497 Size: 393 386 16 710 421 105 170 57 Cuts at: 3497 3621 3633 3843 4637 5234 6021 6289 1239 Size: 124 12 210 794 597 787 268 1524 Fragments arranged by size: 1524 794 787 710 597 421 393 386 268 210 170 124 105 57 16 12 EcoRI G'AATT_C Cuts at: 3591 3883 4261 3591 Size: 292 378 5904 Fragments arranged by size: 5904 378 292 EcoRV GAT'ATC Cuts at: 2894 2894 Size: 6574 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3059 3383 3059 Size: 324 6250 FspI TGC'GCA Cuts at: 5080 5080 Size: 6574 HaeII r_GCGC'y Cuts at: 1678 5721 1678 Size: 4043 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3722 3754 5215 5247 5289 Size: 32 135 32 2150 32 1461 32 42 Cuts at: 5289 5321 1373 Size: 32 2626 Fragments arranged by size: 2626 2150 1461 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2125 3918 4164 669 Size: 988 281 187 1793 246 3079 Fragments arranged by size: 3079 1793 988 281 246 187 HindIII A'AGCT_T Cuts at: 748 748 Size: 6574 HpaI GTT'AAC Cuts at: 4164 4164 Size: 6574 KpnI G_GTAC'C Cuts at: 3899 3899 Size: 6574 MfeI C'AATT_G Cuts at: 4173 4173 Size: 6574 MluI A'CGCG_T Cuts at: 6450 6450 Size: 6574 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5688 5872 1305 Size: 4383 184 2007 Fragments arranged by size: 4383 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4752 Fragments arranged by size: 4752 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2537 3057 3321 3543 3783 4532 4891 Size: 648 1371 520 264 222 240 749 359 Cuts at: 4891 5050 518 Size: 159 2042 Fragments arranged by size: 2042 1371 749 648 520 359 264 240 222 159 MspA1I CmG'CkG Cuts at: 1606 2764 3213 3990 4667 5815 6060 1606 Size: 1158 449 777 677 1148 245 2120 Fragments arranged by size: 2120 1158 1148 777 677 449 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5083 Fragments arranged by size: 5083 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6574 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6574 NotI GC'GGCC_GC Cuts at: 4021 4021 Size: 6574 NspI r_CATG'y Cuts at: 1132 1229 2774 3138 3443 3623 3638 3932 5477 Size: 97 1545 364 305 180 15 294 1545 Cuts at: 5477 1132 Size: 2229 Fragments arranged by size: 2229 1545 1545 364 305 294 180 97 15 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6574 PciI A'CATG_T Cuts at: 2770 3619 5473 2770 Size: 849 1854 3871 Fragments arranged by size: 3871 1854 849 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6574 PfoI T'CCnGG_A Cuts at: 3513 3513 Size: 6574 PmeI GTTT'AAAC Cuts at: 3878 3878 Size: 6574 PmlI CAC'GTG Cuts at: 3383 3703 3383 Size: 320 6254 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2599 2631 4643 4675 6156 6188 2599 Size: 32 2012 32 1481 32 2985 Fragments arranged by size: 2985 2012 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6574 PsiI TTA'TAA Cuts at: 4144 4144 Size: 6574 PstI C_TGCA'G Cuts at: 830 2488 3926 830 Size: 1658 1438 3478 Fragments arranged by size: 3478 1658 1438 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6574 PvuII CAG'CTG Cuts at: 2764 3213 2764 Size: 449 6125 SalI G'TCGA_C Cuts at: 1936 2123 3916 1936 Size: 187 1793 4594 Fragments arranged by size: 4594 1793 187 SbfI CC_TGCA'GG Cuts at: 3926 3926 Size: 6574 ScaI AGT'ACT Cuts at: 2999 4822 6534 2999 Size: 1823 1712 3039 Fragments arranged by size: 3039 1823 1712 SfcI C'TryA_G Cuts at: 826 1045 2484 3922 5057 5738 5929 826 Size: 219 1439 1438 1135 681 191 1471 Fragments arranged by size: 1471 1439 1438 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6574 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6574 SmaI CCC'GGG Cuts at: 1872 3901 1872 Size: 2029 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4678 5579 5841 820 Size: 197 926 2735 901 262 1553 Fragments arranged by size: 2735 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4365 493 Size: 3872 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6574 SphI G_CATG'C Cuts at: 1132 3138 3932 1132 Size: 2006 794 3774 Fragments arranged by size: 3774 2006 794 SspI AAT'ATT Cuts at: 5 52 3337 4498 5 Size: 47 3285 1161 2081 Fragments arranged by size: 3285 2081 1161 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6574 StyI C'CwwG_G Cuts at: 513 1065 2004 4013 513 Size: 552 939 2009 3074 Fragments arranged by size: 3074 2009 939 552 SwaI ATTT'AAAT Cuts at: 2815 2815 Size: 6574 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2085 4601 4618 4771 4956 5401 325 Size: 1255 505 2516 17 153 185 445 1498 Fragments arranged by size: 2516 1498 1255 505 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2598 2997 3269 Size: 274 80 33 51 1443 621 399 272 Cuts at: 3269 3616 3667 4820 6532 96 Size: 347 51 1153 1712 138 Fragments arranged by size: 1712 1443 1153 621 399 347 274 272 138 80 51 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6534 6566 6534 Size: 32 6542 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6574 XbaI T'CTAG_A Cuts at: 3577 3682 3910 3577 Size: 105 228 6241 Fragments arranged by size: 6241 228 105 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3811 3811 Size: 6574 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6574 XmaI C'CCGG_G Cuts at: 1870 3899 1870 Size: 2029 4545 XmnI GAAnn'nnTTC Cuts at: 2031 3042 3363 4703 2031 Size: 1011 321 1340 3902 Fragments arranged by size: 3902 1340 1011 321 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4382 276 Size: 53 83 186 936 2848 2468 Fragments arranged by size: 2848 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI