Restriction map for
pFN21AB7850
(Linear) (Six-base) MAPSORT of: KIBB7850.seq Check: 2019 from: 1 to: 1869
KIBB7850.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 0 119 1869
Size: 119 1750
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 29 739 1435 1616 1628 1838 1869
Size: 13 16 710 696 181 12 210 31
Fragments arranged by size:
710 696 210 181 31 16 13 12
AflIII A'CryG_T
Cuts at: 0 765 1375 1377 1614 1869
Size: 765 610 2 237 255
Fragments arranged by size:
765 610 255 237 2
AgeI A'CCGG_T
Cuts at: 0 92 1869
Size: 92 1777
AleI CACnn'nnGTG
Cuts at: 0 1538 1869
Size: 1538 331
AlwNI CAG_nnn'CTG
Cuts at: 0 493 1750 1869
Size: 493 1257 119
Fragments arranged by size:
1257 493 119
ApaLI G'TGCA_C
Cuts at: 0 1522 1869
Size: 1522 347
ApoI r'AATT_y
Cuts at: 0 181 977 1586 1869
Size: 181 796 609 283
Fragments arranged by size:
796 609 283 181
AseI AT'TA_AT
Cuts at: 0 280 1869
Size: 280 1589
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1595 1628 1869
Size: 1595 33 241
Fragments arranged by size:
1595 241 33
BanI G'GyrC_C
Cuts at: 0 523 1869
Size: 523 1346
BbvCI CC'TCA_GC
Cuts at: 0 1754 1784 1869
Size: 1754 30 85
Fragments arranged by size:
1754 85 30
BglII A'GATC_T
Cuts at: 0 1500 1869
Size: 1500 369
Bme1580I G_kGCm'C
Cuts at: 0 1526 1869
Size: 1526 343
BmrI ACTGGGnnnn_n'
Cuts at: 0 977 1869
Size: 977 892
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1160 1265 1492 1869
Size: 1160 105 227 377
Fragments arranged by size:
1160 377 227 105
Bpu10I CC'TnA_GC
Cuts at: 0 1754 1784 1869
Size: 1754 30 85
Fragments arranged by size:
1754 85 30
BsaI GGTCTCn'nnnn_
Cuts at: 0 1789 1869
Size: 1789 80
BsaAI yAC'GTr
Cuts at: 0 1378 1698 1869
Size: 1378 320 171
Fragments arranged by size:
1378 320 171
BsaWI w'CCGG_w
Cuts at: 0 92 1869
Size: 92 1777
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1717 1747 1869
Size: 1717 30 122
Fragments arranged by size:
1717 122 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1636 1738 1869
Size: 1636 102 131
Fragments arranged by size:
1636 131 102
BseYI C'CCAG_C
Cuts at: 0 1208 1869
Size: 1208 661
BsiEI CG_ry'CG
Cuts at: 0 66 1869
Size: 66 1803
BsiHKAI G_wGCw'C
Cuts at: 0 607 836 1526 1869
Size: 607 229 690 343
Fragments arranged by size:
690 607 343 229
BsmI GAATG_Cn'
Cuts at: 0 28 1183 1869
Size: 28 1155 686
Fragments arranged by size:
1155 686 28
BsmBI CGTCTCn'nnnn_
Cuts at: 0 7 1869
Size: 7 1862
Bsp1286I G_dGCh'C
Cuts at: 0 607 836 1526 1869
Size: 607 229 690 343
Fragments arranged by size:
690 607 343 229
BspHI T'CATG_A
Cuts at: 0 1083 1712 1869
Size: 1083 629 157
Fragments arranged by size:
1083 629 157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1545 1869
Size: 1545 324
BsrDI GCAATG_nn'
Cuts at: 0 469 1444 1869
Size: 469 975 425
Fragments arranged by size:
975 469 425
BsrFI r'CCGG_y
Cuts at: 0 92 1869
Size: 92 1777
BssSI C'ACGA_G
Cuts at: 0 717 1869
Size: 717 1152
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1750 1869
Size: 1750 119
BstBI TT'CG_AA
Cuts at: 0 1041 1869
Size: 1041 828
BstXI CCAn_nnnn'nTGG
Cuts at: 0 534 1869
Size: 534 1335
BstYI r'GATC_y
Cuts at: 0 1500 1869
Size: 1500 369
BtsI GCAGTG_nn'
Cuts at: 0 1124 1869
Size: 1124 745
DraI TTT'AAA
Cuts at: 0 810 1029 1869
Size: 810 219 840
Fragments arranged by size:
840 810 219
EarI CTCTTCn'nnn_
Cuts at: 0 5 1869
Size: 5 1864
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 29 739 1160 1265 1435 1492 1616
Size: 13 16 710 421 105 170 57 124
Cuts at: 1616 1628 1838 1869
Size: 12 210 31
Fragments arranged by size:
710 421 210 170 124 105 57 31
16 13 12
EcoRI G'AATT_C
Cuts at: 0 1586 1869
Size: 1586 283
EcoRV GAT'ATC
Cuts at: 0 889 1869
Size: 889 980
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1054 1378 1869
Size: 1054 324 491
Fragments arranged by size:
1054 491 324
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1717 1749 1869
Size: 1717 32 120
Fragments arranged by size:
1717 120 32
HincII GTy'rAC
Cuts at: 0 120 1869
Size: 120 1749
MslI CAynn'nnrTG
Cuts at: 0 532 1052 1316 1538 1778 1869
Size: 532 520 264 222 240 91
Fragments arranged by size:
532 520 264 240 222 91
MspA1I CmG'CkG
Cuts at: 0 759 1208 1869
Size: 759 449 661
Fragments arranged by size:
759 661 449
NspI r_CATG'y
Cuts at: 0 769 1133 1438 1618 1633 1869
Size: 769 364 305 180 15 236
Fragments arranged by size:
769 364 305 236 180 15
PciI A'CATG_T
Cuts at: 0 765 1614 1869
Size: 765 849 255
Fragments arranged by size:
849 765 255
PfoI T'CCnGG_A
Cuts at: 0 1508 1869
Size: 1508 361
PmlI CAC'GTG
Cuts at: 0 1378 1698 1869
Size: 1378 320 171
Fragments arranged by size:
1378 320 171
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 594 626 1869
Size: 594 32 1243
Fragments arranged by size:
1243 594 32
PstI C_TGCA'G
Cuts at: 0 483 1869
Size: 483 1386
PvuII CAG'CTG
Cuts at: 0 759 1208 1869
Size: 759 449 661
Fragments arranged by size:
759 661 449
SalI G'TCGA_C
Cuts at: 0 118 1869
Size: 118 1751
ScaI AGT'ACT
Cuts at: 0 994 1869
Size: 994 875
SfcI C'TryA_G
Cuts at: 0 479 1869
Size: 479 1390
SphI G_CATG'C
Cuts at: 0 1133 1869
Size: 1133 736
SspI AAT'ATT
Cuts at: 0 1332 1869
Size: 1332 537
SwaI ATTT'AAAT
Cuts at: 0 810 1869
Size: 810 1059
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 80 1869
Size: 80 1789
TatI w'GTAC_w
Cuts at: 0 593 992 1264 1611 1662 1869
Size: 593 399 272 347 51 207
Fragments arranged by size:
593 399 347 272 207 51
XbaI T'CTAG_A
Cuts at: 0 1572 1677 1869
Size: 1572 105 192
Fragments arranged by size:
1572 192 105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1806 1869
Size: 1806 63
XmnI GAAnn'nnTTC
Cuts at: 0 26 1037 1358 1869
Size: 26 1011 321 511
Fragments arranged by size:
1011 511 321 26
Enzymes that do cut:
AccI AcuI AflIII AgeI AleI AlwNI ApaLI ApoI
AseI BaeI BanI BbvCI BglII Bme1580I BmrI BpmI
Bpu10I BsaI BsaAI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtsI DraI EarI
Eco57MI EcoRI EcoRV FalI Hin4I HincII MslI MspA1I
NspI PciI PfoI PmlI PpiI PstI PvuII SalI
ScaI SfcI SphI SspI SwaI TaqII TatI XbaI
XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AhdI AlfI
AloI ApaI AscI AsiSI AvaI AvrII BamHI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BlpI
BmgBI BmtI BplI BpuEI BsaBI BsaHI BsgI BsiWI
BspEI BsrBI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoO109I FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PmeI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI StuI StyI TstI Tth111I XhoI
XmaI ZraI