Restriction map for
pFN21AA1789
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# NO CUT ENZYMES ( 179 enzymes) #
##################################
AatII Acc65I AccI AccII AciI
AclI AcyI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BclI
BcnI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaJI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspLU11I
BsrBI BsrFI BsrGI BssHII BssKI
BssSI Bst1107I BstAPI BstBI BstEII
BstNI BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MscI MspA1I
MunI MvaI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
ScrFI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccIII 317 TCCGGA T^CCGGA
AcsI 120 GAATTC [GA]^AATT[CT]
AflII 1264 CTTAAG C^TTAAG
AlwI 448 GATCC GGATC(4/5)
AlwNI 951 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 120 GAATTC [GA]^AATT[CT]
BbvI 328 GCAGC GCAGC(8/12)
BciVI 489 GGATAC GTATCC(6/5)
BfrI 1264 CTTAAG C^TTAAG
BseAI 317 TCCGGA T^CCGGA
BseRI 1231 GAGGAG GAGGAG(10/8)
BsmFI 60 GTCCC GGGAC(10/14)
BspEI 317 TCCGGA T^CCGGA
BspHI 903 TCATGA T^CATGA
BspMI 1081 GCAGGT ACCTGC(4/8)
BstF5I 1380 GGATG GGATG(2/0)
BstYI 447 AGATCC [GA]^GATC[CT]
DdeI 620 CTCAG C^T[ACGT]AG
EcoRI 120 GAATTC G^AATTC
Fnu4HI 328 GCAGC GC^[ACGT]GC
FokI 1380 GGATG GGATG(9/13)
HincII 941 GTCAAC GT[CT]^[GA]AC
HindII 941 GTCAAC GT[CT]^[GA]AC
HindIII 1261 AAGCTT A^AGCTT
ItaI 328 GCAGC GC^[ACGT]GC
MaeII 1317 ACGT A^CGT
MflI 447 AGATCC [GA]^GATC[CT]
MroI 317 TCCGGA T^CCGGA
MwoI 1152 GCAGTCCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 65 ACATGC [GA]CATG^[CT]
RcaI 903 TCATGA T^CATGA
SfcI 749 CTGTAG C^T[GA][CT]AG
SspI 340 AATATT AAT^ATT
TseI 328 GCAGC G^C[AT]GC
XhoII 447 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BbsI 129 GAAGAC GAAGAC(2/6)
381 GAAGAC
BpuAI 129 GAAGAC GAAGAC(2/6)
381 GAAGAC
BsaWI 56 TCCGGT [AT]^CCGG[AT]
317 TCCGGA
BsrDI 889 CATTGC GCAATG(2/0)
352 GCAATG
DpnII 448 GATC ^GATC
530 GATC
HaeIII 198 GGCC GG^CC
1139 GGCC
HapII 57 CCGG C^CGG
318 CCGG
HpaII 57 CCGG C^CGG
318 CCGG
HphI 598 TCACC GGTGA(8/7)
947 TCACC
MboI 448 GATC ^GATC
530 GATC
MslI 168 CATCAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
684 CATAAAAGTG
MspI 57 CCGG C^CGG
318 CCGG
NdeI 386 CATATG CA^TATG
650 CATATG
NdeII 448 GATC ^GATC
530 GATC
NlaIV 59 GGTCCC GG[ACGT]^[ACGT]CC
523 GGACCC
Sau3AI 448 GATC ^GATC
530 GATC
SfaNI 1211 GCATC GCATC(5/9)
1269 GCATC
SmlI 981 CTTGAG C^T[CT][GA]AG
1264 CTTAAG
TaqI 263 TCGA T^CGA
1272 TCGA
Tsp509I 87 AATT ^AATT
121 AATT
TthHB8I 263 TCGA T^CGA
1272 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AfaI AluI AvaII BfaI BsgI
BsmAI BsrI Cfr13I EarI HinfI
Ksp632I MaeI MaeIII MboII MnlI
MseI NlaIII PleI RsaI Sau96I
TfiI Tru9I TspRI