(Circular) (Six-base) MAPSORT of: pFN21AA1789.seq Check: 699 from: 1 to: 6106 pFN21AA1789 6106 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3916 278 Size: 53 83 186 936 2380 2468 Fragments arranged by size: 2468 2380 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3449 1937 Size: 1512 4594 Acc65I G'GTAC_C Cuts at: 3427 3427 Size: 6106 AclI AA'CG_TT Cuts at: 1563 4233 4606 1563 Size: 2670 373 3063 Fragments arranged by size: 3063 2670 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3042 3057 4169 5553 5821 1632 Size: 1410 15 1112 1384 268 1917 Fragments arranged by size: 1917 1410 1384 1112 268 15 AflII C'TTAA_G Cuts at: 820 1017 3269 820 Size: 197 2252 3657 Fragments arranged by size: 3657 2252 197 AflIII A'CryG_T Cuts at: 1203 5005 5982 1203 Size: 3802 977 1327 Fragments arranged by size: 3802 1327 977 AgeI A'CCGG_T Cuts at: 4937 4937 Size: 6106 AhdI GACnn_n'nnGTC Cuts at: 4835 4835 Size: 6106 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6106 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6106 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2289 2321 2289 Size: 32 6074 AlwNI CAG_nnn'CTG Cuts at: 2961 3849 5421 2961 Size: 888 1572 3646 Fragments arranged by size: 3646 1572 888 ApaLI G'TGCA_C Cuts at: 4163 5319 4163 Size: 1156 4950 ApoI r'AATT_y Cuts at: 1493 2125 3415 3641 3793 5902 1493 Size: 632 1290 226 152 2109 1697 Fragments arranged by size: 2109 1697 1290 632 226 152 AseI AT'TA_AT Cuts at: 160 1032 4660 160 Size: 872 3628 1606 Fragments arranged by size: 3628 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 AvaI C'yCGr_G Cuts at: 1870 1943 3431 1870 Size: 73 1488 4545 Fragments arranged by size: 4545 1488 73 BamHI G'GATC_C Cuts at: 3436 3803 3436 Size: 367 5739 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3427 4882 618 Size: 325 217 627 1640 1455 1842 Fragments arranged by size: 1842 1640 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3425 1391 Size: 2034 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2141 2393 928 Size: 971 242 252 4641 Fragments arranged by size: 4641 971 252 242 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4297 4331 1903 Size: 34 2360 34 3678 Fragments arranged by size: 3678 2360 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2488 3998 5208 2488 Size: 1510 1210 3386 Fragments arranged by size: 3386 1510 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6106 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4717 136 Size: 107 122 71 631 605 3045 1525 Fragments arranged by size: 3045 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6101 6101 Size: 6106 BlpI GC'TnA_GC Cuts at: 3523 3523 Size: 6106 Bme1580I G_kGCm'C Cuts at: 1790 4167 5323 1790 Size: 2377 1156 2573 Fragments arranged by size: 2573 2377 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6106 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3837 4795 442 Size: 416 53 83 399 24 2420 958 1753 Fragments arranged by size: 2420 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6106 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4766 1239 Size: 3527 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3007 4231 5096 5394 3007 Size: 1224 865 298 3719 Fragments arranged by size: 3719 1224 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4769 882 Size: 617 3270 2219 Fragments arranged by size: 3270 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3897 493 Size: 711 2693 2702 Fragments arranged by size: 2702 2693 711 BsaBI GATnn'nnATC Cuts at: 6100 6100 Size: 6106 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3913 4295 4930 275 Size: 53 83 186 936 2380 382 635 1451 Fragments arranged by size: 2380 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2061 2322 4539 4937 5211 5358 5769 2061 Size: 261 2217 398 274 147 411 2398 Fragments arranged by size: 2398 2217 411 398 274 261 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2289 2319 1178 Size: 30 165 30 886 30 4965 Fragments arranged by size: 4965 886 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3251 1186 Size: 2065 4041 BseYI C'CCAG_C Cuts at: 5309 5805 5309 Size: 496 5610 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2184 2201 2717 1163 Size: 573 448 17 516 4552 Fragments arranged by size: 4552 573 516 448 17 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3556 4317 5345 1540 Size: 275 186 1555 761 1028 2301 Fragments arranged by size: 2301 1555 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4167 4252 5323 4167 Size: 85 1071 4950 Fragments arranged by size: 4950 1071 85 BsmI GAATG_Cn' Cuts at: 3617 3710 3617 Size: 93 6013 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3425 4167 4252 5323 1391 Size: 399 1635 742 85 1071 2174 Fragments arranged by size: 2174 1635 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2322 5769 2322 Size: 3447 2659 BspHI T'CATG_A Cuts at: 2908 3995 5725 2908 Size: 1087 1730 3289 Fragments arranged by size: 3289 1730 1087 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3077 3461 844 Size: 2233 384 3489 Fragments arranged by size: 3489 2233 384 BsrBI CCG'CTC Cuts at: 1394 1823 3993 1394 Size: 429 2170 3507 Fragments arranged by size: 3507 2170 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2364 2893 4601 4775 65 Size: 1183 1116 529 1708 174 1396 Fragments arranged by size: 1708 1396 1183 1116 529 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4750 4937 1700 Size: 110 2940 187 2869 Fragments arranged by size: 2940 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6106 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6106 BssSI C'ACGA_G Cuts at: 4166 5178 4166 Size: 1012 5094 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6106 BstBI TT'CG_AA Cuts at: 4910 4910 Size: 6106 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6106 BstYI r'GATC_y Cuts at: 1972 2452 3436 3803 4188 4205 5657 5743 5755 Size: 480 984 367 385 17 1452 86 12 Cuts at: 5755 6101 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 984 480 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5554 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5367 Fragments arranged by size: 5367 683 56 BtsI GCAGTG_nn' Cuts at: 3622 4416 4436 3622 Size: 794 20 5292 Fragments arranged by size: 5292 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6106 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6071 DraI TTT'AAA Cuts at: 3410 3410 Size: 6106 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5113 809 Size: 507 3797 1802 Fragments arranged by size: 3797 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3553 4442 6071 Size: 54 1425 50 275 18 1723 889 1629 Cuts at: 6071 8 Size: 43 Fragments arranged by size: 1723 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3553 1537 Size: 275 1741 4090 Fragments arranged by size: 4090 1741 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2223 3061 3104 3363 4036 1353 Size: 870 838 43 259 673 3423 Fragments arranged by size: 3423 870 838 673 259 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4693 5067 5213 387 Size: 1064 138 3104 374 146 1280 Fragments arranged by size: 3104 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3042 3057 4169 4766 5553 5821 1239 Size: 393 1410 15 1112 597 787 268 1524 Fragments arranged by size: 1524 1410 1112 787 597 393 268 15 EcoRI G'AATT_C Cuts at: 2125 3415 3793 2125 Size: 1290 378 4438 Fragments arranged by size: 4438 1290 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2429 2693 2429 Size: 264 5842 FspI TGC'GCA Cuts at: 4612 4612 Size: 6106 HaeII r_GCGC'y Cuts at: 1678 5253 1678 Size: 3575 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2250 2282 2980 3012 3237 Size: 32 135 32 678 32 698 32 225 Cuts at: 3237 3269 3290 3322 4747 4779 4821 4853 1373 Size: 32 21 32 1425 32 42 32 2626 Fragments arranged by size: 2626 1425 698 678 225 135 42 32 32 32 32 32 32 32 32 21 HincII GTy'rAC Cuts at: 669 1657 1938 2948 3450 3696 669 Size: 988 281 1010 502 246 3079 Fragments arranged by size: 3079 1010 988 502 281 246 HindIII A'AGCT_T Cuts at: 748 3266 748 Size: 2518 3588 HpaI GTT'AAC Cuts at: 3696 3696 Size: 6106 KpnI G_GTAC'C Cuts at: 3431 3431 Size: 6106 MfeI C'AATT_G Cuts at: 3705 3705 Size: 6106 MluI A'CGCG_T Cuts at: 5982 5982 Size: 6106 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2790 5220 5404 1305 Size: 1485 2430 184 2007 Fragments arranged by size: 2430 2007 1485 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4284 Fragments arranged by size: 4284 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2177 2693 4064 4423 4582 518 Size: 648 1011 516 1371 359 159 2042 Fragments arranged by size: 2042 1371 1011 648 516 359 159 MspA1I CmG'CkG Cuts at: 1606 3522 4199 5347 5592 1606 Size: 1916 677 1148 245 2120 Fragments arranged by size: 2120 1916 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5554 NdeI CA'TA_TG Cuts at: 387 2392 2656 387 Size: 2005 264 3837 Fragments arranged by size: 3837 2005 264 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6106 NotI GC'GGCC_GC Cuts at: 3553 3553 Size: 6106 NspI r_CATG'y Cuts at: 1132 1229 2074 3464 5009 1132 Size: 97 845 1390 1545 2229 Fragments arranged by size: 2229 1545 1390 845 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6106 PciI A'CATG_T Cuts at: 5005 5005 Size: 6106 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6106 PmeI GTTT'AAAC Cuts at: 3410 3410 Size: 6106 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2289 2321 4175 4207 5688 5720 2289 Size: 32 1854 32 1481 32 2675 Fragments arranged by size: 2675 1854 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6106 PsiI TTA'TAA Cuts at: 3676 3676 Size: 6106 PstI C_TGCA'G Cuts at: 830 3458 830 Size: 2628 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6106 SalI G'TCGA_C Cuts at: 1936 3448 1936 Size: 1512 4594 SbfI CC_TGCA'GG Cuts at: 3458 3458 Size: 6106 ScaI AGT'ACT Cuts at: 4354 6066 4354 Size: 1712 4394 SfcI C'TryA_G Cuts at: 826 1045 2754 3454 4589 5270 5461 826 Size: 219 1709 700 1135 681 191 1471 Fragments arranged by size: 1709 1471 1135 700 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6106 SmaI CCC'GGG Cuts at: 1872 3433 1872 Size: 1561 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2986 3269 4210 5111 5373 820 Size: 197 926 1043 283 941 901 262 1553 Fragments arranged by size: 1553 1043 941 926 901 283 262 197 SnaBI TAC'GTA Cuts at: 493 3897 493 Size: 3404 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6106 SphI G_CATG'C Cuts at: 1132 3464 1132 Size: 2332 3774 SspI AAT'ATT Cuts at: 5 52 2347 4030 5 Size: 47 2295 1683 2081 Fragments arranged by size: 2295 2081 1683 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6106 StyI C'CwwG_G Cuts at: 513 1065 3545 513 Size: 552 2480 3074 Fragments arranged by size: 3074 2480 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4133 4150 4303 4488 4933 325 Size: 1255 2553 17 153 185 445 1498 Fragments arranged by size: 2553 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2431 4352 6064 Size: 274 80 33 51 1443 454 1921 1712 Cuts at: 6064 96 Size: 138 Fragments arranged by size: 1921 1712 1443 454 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6066 6098 6066 Size: 32 6074 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6106 XbaI T'CTAG_A Cuts at: 3442 3442 Size: 6106 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6106 XmaI C'CCGG_G Cuts at: 1870 3431 1870 Size: 1561 4545 XmnI GAAnn'nnTTC Cuts at: 4235 4235 Size: 6106 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3914 276 Size: 53 83 186 936 2380 2468 Fragments arranged by size: 2468 2380 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI