(Circular) (Six-base) MAPSORT of: pFN21AB7757.seq Check: 9269 from: 1 to: 6046 pFN21AB7757 6046 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3856 278 Size: 53 83 186 936 2320 2468 Fragments arranged by size: 2468 2320 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3389 1937 Size: 1452 4594 Acc65I G'GTAC_C Cuts at: 3367 3367 Size: 6046 AclI AA'CG_TT Cuts at: 1563 4173 4546 1563 Size: 2610 373 3063 Fragments arranged by size: 3063 2610 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4109 5493 5761 1632 Size: 2477 1384 268 1917 Fragments arranged by size: 2477 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5849 AflIII A'CryG_T Cuts at: 1203 2463 2514 4945 5922 1203 Size: 1260 51 2431 977 1327 Fragments arranged by size: 2431 1327 1260 977 51 AgeI A'CCGG_T Cuts at: 4877 4877 Size: 6046 AhdI GACnn_n'nnGTC Cuts at: 4775 4775 Size: 6046 AleI CACnn'nnGTG Cuts at: 1166 2787 3230 1166 Size: 1621 443 3982 Fragments arranged by size: 3982 1621 443 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2589 1184 Size: 1405 4641 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2291 2323 2291 Size: 32 6014 AlwNI CAG_nnn'CTG Cuts at: 3789 5361 3789 Size: 1572 4474 ApaI G_GGCC'C Cuts at: 2396 2601 2396 Size: 205 5841 ApaLI G'TGCA_C Cuts at: 2888 3068 4103 5259 2888 Size: 180 1035 1156 3675 Fragments arranged by size: 3675 1156 1035 180 ApoI r'AATT_y Cuts at: 1493 2956 3355 3581 3733 5842 1493 Size: 1463 399 226 152 2109 1697 Fragments arranged by size: 2109 1697 1463 399 226 152 AseI AT'TA_AT Cuts at: 160 1032 4600 160 Size: 872 3568 1606 Fragments arranged by size: 3568 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 AvaI C'yCGr_G Cuts at: 1870 1943 2251 2435 2506 2699 3371 1870 Size: 73 308 184 71 193 672 4545 Fragments arranged by size: 4545 672 308 193 184 73 71 BamHI G'GATC_C Cuts at: 3376 3743 3376 Size: 367 5679 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2138 2206 2630 2636 3367 Size: 325 217 627 351 68 424 6 731 Cuts at: 3367 4822 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 731 627 424 351 325 217 68 6 BanII G_rGCy'C Cuts at: 1391 2396 2601 2679 2700 3176 3365 1391 Size: 1005 205 78 21 476 189 4072 Fragments arranged by size: 4072 1005 476 205 189 78 21 BbeI G_GCGC'C Cuts at: 2142 2210 2634 2640 2142 Size: 68 424 6 5548 Fragments arranged by size: 5548 424 68 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2932 928 Size: 971 1033 4042 Fragments arranged by size: 4042 1033 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2435 2469 2674 2708 2923 Size: 34 50 34 414 34 205 34 215 Cuts at: 2923 2957 4237 4271 1903 Size: 34 1280 34 3678 Fragments arranged by size: 3678 1280 414 215 205 50 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3938 5148 3938 Size: 1210 4836 BclI T'GATC_A Cuts at: 1548 1548 Size: 6046 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2262 2400 4657 Size: 107 122 71 631 605 590 138 2257 Cuts at: 4657 136 Size: 1525 Fragments arranged by size: 2257 1525 631 605 590 138 122 107 71 BglII A'GATC_T Cuts at: 6041 6041 Size: 6046 BlpI GC'TnA_GC Cuts at: 3463 3463 Size: 6046 Bme1580I G_kGCm'C Cuts at: 1790 2396 2601 2892 3072 4107 5263 1790 Size: 606 205 291 180 1035 1156 2573 Fragments arranged by size: 2573 1156 1035 606 291 205 180 BmgBI CAC'GTC Cuts at: 1318 2086 2101 2199 2260 2770 1318 Size: 768 15 98 61 510 4594 Fragments arranged by size: 4594 768 510 98 61 15 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2020 2151 3120 Size: 416 53 83 399 24 603 131 969 Cuts at: 3120 3777 4735 442 Size: 657 958 1753 Fragments arranged by size: 1753 969 958 657 603 416 399 131 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6046 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4706 1239 Size: 3467 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4171 5036 5334 4171 Size: 865 298 4883 Fragments arranged by size: 4883 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4709 882 Size: 617 3210 2219 Fragments arranged by size: 3210 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2785 3067 3837 493 Size: 711 1581 282 770 2702 Fragments arranged by size: 2702 1581 770 711 282 BsaBI GATnn'nnATC Cuts at: 6040 6040 Size: 6046 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2016 2139 2207 2412 Size: 53 83 186 936 483 123 68 205 Cuts at: 2412 2631 2637 3853 4235 4870 275 Size: 219 6 1216 382 635 1451 Fragments arranged by size: 1451 1216 936 635 483 382 219 205 186 123 83 68 53 6 BsaWI w'CCGG_w Cuts at: 2527 4479 4877 5151 5298 5709 2527 Size: 1952 398 274 147 411 2864 Fragments arranged by size: 2864 1952 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2249 2279 3146 3176 3197 Size: 30 165 30 846 30 867 30 21 Cuts at: 3197 3227 1178 Size: 30 3997 Fragments arranged by size: 3997 867 846 165 30 30 30 30 30 21 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2417 2840 3154 3268 3271 1186 Size: 1231 423 314 114 3 3961 Fragments arranged by size: 3961 1231 423 314 114 3 BseYI C'CCAG_C Cuts at: 2032 5249 5745 2032 Size: 3217 496 2333 Fragments arranged by size: 3217 2333 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2570 1163 Size: 573 834 4639 Fragments arranged by size: 4639 834 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2040 2181 2379 2528 3049 3192 Size: 275 186 39 141 198 149 521 143 Cuts at: 3192 3496 4257 5285 1540 Size: 304 761 1028 2301 Fragments arranged by size: 2301 1028 761 521 304 275 198 186 149 143 141 39 BsiHKAI G_wGCw'C Cuts at: 2568 2679 2784 2892 3072 4107 4192 5263 2568 Size: 111 105 108 180 1035 85 1071 3351 Fragments arranged by size: 3351 1071 1035 180 111 108 105 85 BsmI GAATG_Cn' Cuts at: 3557 3650 3557 Size: 93 5953 BsmBI CGTCTCn'nnnn_ Cuts at: 2091 2091 Size: 6046 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2396 2568 2601 2679 2700 2784 2892 Size: 399 606 172 33 78 21 84 108 Cuts at: 2892 3072 3176 3365 4107 4192 5263 1391 Size: 180 104 189 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 606 399 189 180 172 108 104 85 84 78 33 21 BspEI T'CCGG_A Cuts at: 5709 5709 Size: 6046 BspHI T'CATG_A Cuts at: 3935 5665 3935 Size: 1730 4316 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3401 844 Size: 2557 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3933 1394 Size: 429 2110 3507 Fragments arranged by size: 3507 2110 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4541 4715 65 Size: 1183 3293 174 1396 Fragments arranged by size: 3293 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2018 2180 2456 2633 3024 3187 3302 Size: 110 208 162 276 177 391 163 115 Cuts at: 3302 4690 4877 1700 Size: 1388 187 2869 Fragments arranged by size: 2869 1388 391 276 208 187 177 163 162 115 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6046 BssHII G'CGCG_C Cuts at: 1925 2945 1925 Size: 1020 5026 BssSI C'ACGA_G Cuts at: 2165 2444 2753 3073 4106 5118 2165 Size: 279 309 320 1033 1012 3093 Fragments arranged by size: 3093 1033 1012 320 309 279 BstAPI GCAn_nnn'nTGC Cuts at: 794 3102 794 Size: 2308 3738 BstBI TT'CG_AA Cuts at: 4850 4850 Size: 6046 BstEII G'GTnAC_C Cuts at: 1184 2545 1184 Size: 1361 4685 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6046 BstYI r'GATC_y Cuts at: 1972 2964 3376 3743 4128 4145 5597 5683 5695 Size: 992 412 367 385 17 1452 86 12 Cuts at: 5695 6041 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 992 412 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2076 2415 2775 513 Size: 552 1011 339 360 3784 Fragments arranged by size: 3784 1011 552 360 339 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5307 Fragments arranged by size: 5307 683 56 BtsI GCAGTG_nn' Cuts at: 3562 4356 4376 3562 Size: 794 20 5232 Fragments arranged by size: 5232 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6046 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6011 DraI TTT'AAA Cuts at: 2961 3350 2961 Size: 389 5657 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5053 809 Size: 507 3737 1802 Fragments arranged by size: 3737 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2088 2178 2182 Size: 54 1425 50 275 18 258 90 4 Cuts at: 2182 2376 2401 2458 2539 2616 2709 3189 3304 Size: 194 25 57 81 77 93 480 115 Cuts at: 3304 3493 4382 6011 8 Size: 189 889 1629 43 Fragments arranged by size: 1629 1425 889 480 275 258 194 189 115 93 90 81 77 57 54 50 43 25 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2178 2376 3189 3493 1537 Size: 275 366 198 813 304 4090 Fragments arranged by size: 4090 813 366 304 275 198 EarI CTCTTCn'nnn_ Cuts at: 1353 2435 3325 3976 1353 Size: 1082 890 651 3423 Fragments arranged by size: 3423 1082 890 651 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4633 5007 5153 387 Size: 1064 138 3044 374 146 1280 Fragments arranged by size: 3044 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2677 2677 Size: 6046 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4109 4706 5493 5761 1239 Size: 393 2477 597 787 268 1524 Fragments arranged by size: 2477 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2392 2392 Size: 6046 EcoRI G'AATT_C Cuts at: 3355 3733 3355 Size: 378 5668 FseI GG_CCGG'CC Cuts at: 2184 2184 Size: 6046 FspI TGC'GCA Cuts at: 3137 4552 3137 Size: 1415 4631 HaeII r_GCGC'y Cuts at: 1678 2142 2210 2634 2640 3031 5193 1678 Size: 464 68 424 6 391 2162 2531 Fragments arranged by size: 2531 2162 464 424 391 68 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4687 4719 4761 4793 1373 Size: 32 135 32 3115 32 42 32 2626 Fragments arranged by size: 3115 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2482 2875 3390 3636 669 Size: 988 281 544 393 515 246 3079 Fragments arranged by size: 3079 988 544 515 393 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6046 HpaI GTT'AAC Cuts at: 2875 3636 2875 Size: 761 5285 KasI G'GCGC_C Cuts at: 2138 2206 2630 2636 2138 Size: 68 424 6 5548 Fragments arranged by size: 5548 424 68 6 KpnI G_GTAC'C Cuts at: 3371 3371 Size: 6046 MfeI C'AATT_G Cuts at: 3645 3645 Size: 6046 MluI A'CGCG_T Cuts at: 2463 5922 2463 Size: 3459 2587 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5160 5344 1305 Size: 3855 184 2007 Fragments arranged by size: 3855 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2711 10 Size: 54 1425 343 879 3345 Fragments arranged by size: 3345 1425 879 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2606 2787 2885 3065 3230 4004 4363 Size: 648 1440 181 98 180 165 774 359 Cuts at: 4363 4522 518 Size: 159 2042 Fragments arranged by size: 2042 1440 774 648 359 181 180 165 159 98 MspA1I CmG'CkG Cuts at: 1606 2078 2417 2584 2707 3462 4139 5287 5532 Size: 472 339 167 123 755 677 1148 245 Cuts at: 5532 1606 Size: 2120 Fragments arranged by size: 2120 1148 755 677 472 339 245 167 123 NaeI GCC'GGC Cuts at: 2020 2182 2458 2635 3189 3304 2020 Size: 162 276 177 554 115 4762 Fragments arranged by size: 4762 554 276 177 162 115 NarI GG'CG_CC Cuts at: 2139 2207 2631 2637 2139 Size: 68 424 6 5548 Fragments arranged by size: 5548 424 68 6 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5494 NdeI CA'TA_TG Cuts at: 387 387 Size: 6046 NgoMIV G'CCGG_C Cuts at: 2018 2180 2456 2633 3187 3302 2018 Size: 162 276 177 554 115 4762 Fragments arranged by size: 4762 554 276 177 162 115 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6046 NotI GC'GGCC_GC Cuts at: 3493 3493 Size: 6046 NspI r_CATG'y Cuts at: 1132 1229 2518 2656 3064 3404 4949 1132 Size: 97 1289 138 408 340 1545 2229 Fragments arranged by size: 2229 1545 1289 408 340 138 97 PasI CC'CwG_GG Cuts at: 1348 2233 1348 Size: 885 5161 PciI A'CATG_T Cuts at: 2514 4945 2514 Size: 2431 3615 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6046 PfoI T'CCnGG_A Cuts at: 2010 2010 Size: 6046 PmeI GTTT'AAAC Cuts at: 3350 3350 Size: 6046 PmlI CAC'GTG Cuts at: 3067 3067 Size: 6046 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4115 4147 5628 5660 4115 Size: 32 1481 32 4501 Fragments arranged by size: 4501 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6046 PsiI TTA'TAA Cuts at: 3616 3616 Size: 6046 PspOMI G'GGCC_C Cuts at: 2392 2597 2392 Size: 205 5841 PstI C_TGCA'G Cuts at: 830 3398 830 Size: 2568 3478 PvuI CG_AT'CG Cuts at: 2001 2040 2001 Size: 39 6007 PvuII CAG'CTG Cuts at: 2584 2707 2584 Size: 123 5923 SacI G_AGCT'C Cuts at: 2679 2679 Size: 6046 SacII CC_GC'GG Cuts at: 2079 2418 2079 Size: 339 5707 SalI G'TCGA_C Cuts at: 1936 3388 1936 Size: 1452 4594 SbfI CC_TGCA'GG Cuts at: 3398 3398 Size: 6046 ScaI AGT'ACT Cuts at: 4294 6006 4294 Size: 1712 4334 SfcI C'TryA_G Cuts at: 826 1045 3394 4529 5210 5401 826 Size: 219 2349 1135 681 191 1471 Fragments arranged by size: 2349 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2400 2400 Size: 6046 SfoI GGC'GCC Cuts at: 2140 2208 2632 2638 2140 Size: 68 424 6 5548 Fragments arranged by size: 5548 424 68 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 SgrAI Cr'CCGG_yG Cuts at: 1700 2633 1700 Size: 933 5113 SmaI CCC'GGG Cuts at: 1872 2253 2437 3373 1872 Size: 381 184 936 4545 Fragments arranged by size: 4545 936 381 184 SmlI C'TyrA_G Cuts at: 820 1017 1943 4150 5051 5313 820 Size: 197 926 2207 901 262 1553 Fragments arranged by size: 2207 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3837 493 Size: 3344 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6046 SphI G_CATG'C Cuts at: 1132 3404 1132 Size: 2272 3774 SspI AAT'ATT Cuts at: 5 52 3970 5 Size: 47 3918 2081 Fragments arranged by size: 3918 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6046 StyI C'CwwG_G Cuts at: 513 1065 3485 513 Size: 552 2420 3074 Fragments arranged by size: 3074 2420 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4073 4090 4243 4428 4873 325 Size: 1255 2493 17 153 185 445 1498 Fragments arranged by size: 2493 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4292 6004 96 Size: 274 80 33 51 1443 2315 1712 138 Fragments arranged by size: 2315 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2249 2281 3146 3178 6006 6038 2249 Size: 32 865 32 2828 32 2257 Fragments arranged by size: 2828 2257 865 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6046 XbaI T'CTAG_A Cuts at: 3382 3382 Size: 6046 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2866 2866 Size: 6046 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6046 XmaI C'CCGG_G Cuts at: 1870 2251 2435 3371 1870 Size: 381 184 936 4545 Fragments arranged by size: 4545 936 381 184 XmnI GAAnn'nnTTC Cuts at: 2996 4175 2996 Size: 1179 4867 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3854 276 Size: 53 83 186 936 2320 2468 Fragments arranged by size: 2468 2320 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FspAI NruI NsiI PacI PpuMI PspXI PsrI RsrII SanDI SapI SexAI SrfI SwaI