Restriction map for
pFN21AA0395
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# NO CUT ENZYMES ( 109 enzymes) #
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Acc65I AccII AccIII AclI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BcgI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsoBI Bsp1407I
BspDI BspEI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV FbaI FseI
FspI HaeII HgaI HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SspBI SspI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AatII 2849 GACGTC GACGT^C
AccI 654 GTCGAC GT^[AC][GT]AC
AcsI 593 AAATTC [GA]^AATT[CT]
AcyI 2849 GACGTC G[GA]^CG[CT]C
AflIII 388 ACATGT A^C[GA][CT]GT
AhdI 1271 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 593 AAATTC [GA]^AATT[CT]
AspEI 1271 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 1026 GTATCC GTATCC(6/5)
BglII 1046 AGATCT A^GATCT
BsaHI 2849 GACGTC G[GA]^CG[CT]C
BsgI 339 GTGCAG GTGCAG(16/14)
BsmBI 1986 GAGACG CGTCTC(1/5)
BspLU11I 388 ACATGT A^CATGT
BsrBI 1223 CCGCTC CCGCTC(-3/-3)
Eam1105I 1271 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 30 ATGCAT ATGCA^T
Hin1I 2849 GACGTC G[GA]^CG[CT]C
HincII 654 GTCGAC GT[CT]^[GA]AC
HindII 654 GTCGAC GT[CT]^[GA]AC
MslI 1581 CACAAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 935 CCATGG C^CATGG
NdeI 283 CATATG CA^TATG
NheI 1480 GCTAGC G^CTAGC
NsiI 30 ATGCAT ATGCA^T
PshAI 1341 GACATCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 654 GTCGAC G^TCGAC
SapI 1225 GCTCTTC GCTCTTC(1/4)
SbfI 2547 CCTGCAGG CCTGCA^GG
ScaI 172 AGTACT AGT^ACT
SphI 840 GCATGC GCATG^C
Sse8387I 2547 CCTGCAGG CCTGCA^GG
StuI 638 AGGCCT AGG^CCT
TfiI 2226 GATTC G^A[AT]TC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwNI 115 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
862 CAGCCACTG
ApaI 763 GGGCCC GGGCC^C
2706 GGGCCC
BbsI 45 GAAGAC GAAGAC(2/6)
2482 GAAGAC
BpuAI 45 GAAGAC GAAGAC(2/6)
2482 GAAGAC
BspHI 557 TCATGA T^CATGA
1506 TCATGA
BsrDI 2523 CATTGC GCAATG(2/0)
1199 GCAATG
BstEII 1017 GGTGACC G^GT[ACGT]ACC
1752 GGTAACC
BstPI 1017 GGTGACC G^GT[ACGT]ACC
1752 GGTAACC
BstXI 2672 CCAGAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2703 CCAGGGCCCTGG
CfoI 2157 GCGC GCG^C
2427 GCGC
DraIII 1237 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
1380 CACTGTGTG
EcoO65I 1017 GGTGACC G^GT[ACGT]ACC
1752 GGTAACC
FauI 85 CCCGC CCCGC(4/6)
493 GCGGG
HhaI 2157 GCGC GCG^C
2427 GCGC
HinP1I 2157 GCGC G^CGC
2427 GCGC
NspI 388 ACATGT [GA]CATG^[CT]
840 GCATGC
PstI 2548 CTGCAG CTGCA^G
2593 CTGCAG
RcaI 557 TCATGA T^CATGA
1506 TCATGA
SfcI 2548 CTGCAG C^T[GA][CT]AG
2593 CTGCAG
SmlI 604 CTCAAG C^T[CT][GA]AG
1042 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
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AciI AfaI AluI AlwI AocI
Asp700I AspHI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BmyI BpmI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI