(Circular) (Six-base) MAPSORT of: pFN21AA0395.seq Check: 8225 from: 1 to: 7573 pFN21AA0395 7573 bp With 182 enzymes: SgfI * March 28, 2012 12:09 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4858 5383 278 Size: 53 83 186 936 3322 525 2468 Fragments arranged by size: 3322 2468 936 525 186 83 53 AccI GT'mk_AC Cuts at: 1937 2660 4916 1937 Size: 723 2256 4594 Fragments arranged by size: 4594 2256 723 Acc65I G'GTAC_C Cuts at: 4894 4894 Size: 7573 AclI AA'CG_TT Cuts at: 1563 5700 6073 1563 Size: 4137 373 3063 Fragments arranged by size: 4137 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2127 2535 2607 2981 3095 4211 4230 4481 Size: 495 408 72 374 114 1116 19 251 Cuts at: 4481 5636 7020 7288 1632 Size: 1155 1384 268 1917 Fragments arranged by size: 1917 1384 1155 1116 495 408 374 268 251 114 72 19 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7376 AflIII A'CryG_T Cuts at: 1203 2393 6472 7449 1203 Size: 1190 4079 977 1327 Fragments arranged by size: 4079 1327 1190 977 AgeI A'CCGG_T Cuts at: 6404 6404 Size: 7573 AhdI GACnn_n'nnGTC Cuts at: 3281 6302 3281 Size: 3021 4552 AleI CACnn'nnGTG Cuts at: 1166 3590 1166 Size: 2424 5149 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4668 1184 Size: 3484 4089 AlwNI CAG_nnn'CTG Cuts at: 2125 2872 5316 6888 2125 Size: 747 2444 1572 2810 Fragments arranged by size: 2810 2444 1572 747 ApaI G_GGCC'C Cuts at: 2772 4715 2772 Size: 1943 5630 ApaLI G'TGCA_C Cuts at: 5630 6786 5630 Size: 1156 6417 ApoI r'AATT_y Cuts at: 1493 2598 4882 5108 5260 7369 1493 Size: 1105 2284 226 152 2109 1697 Fragments arranged by size: 2284 2109 1697 1105 226 152 AseI AT'TA_AT Cuts at: 160 1032 6127 160 Size: 872 5095 1606 Fragments arranged by size: 5095 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7573 AvaI C'yCGr_G Cuts at: 1870 1943 4898 1870 Size: 73 2955 4545 Fragments arranged by size: 4545 2955 73 BamHI G'GATC_C Cuts at: 4903 5270 4903 Size: 367 7206 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3373 3819 4244 4894 6349 Size: 325 217 627 1586 446 425 650 1455 Cuts at: 6349 618 Size: 1842 Fragments arranged by size: 1842 1586 1455 650 627 446 425 325 217 BanII G_rGCy'C Cuts at: 1391 2508 2772 3006 3668 3865 3883 4551 4631 Size: 1117 264 234 662 197 18 668 80 Cuts at: 4631 4715 4731 4800 4827 4892 1391 Size: 84 16 69 27 65 4072 Fragments arranged by size: 4072 1117 668 662 264 234 197 84 80 69 65 27 18 16 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2057 4494 928 Size: 971 158 2437 4007 Fragments arranged by size: 4007 2437 971 158 BbvCI CC'TCA_GC Cuts at: 3156 4011 4029 4065 3156 Size: 855 18 36 6664 Fragments arranged by size: 6664 855 36 18 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5764 5798 1903 Size: 34 3827 34 3678 Fragments arranged by size: 3827 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3042 5465 6675 3042 Size: 2423 1210 3940 Fragments arranged by size: 3940 2423 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7573 BfrBI ATG'CAT Cuts at: 2037 2037 Size: 7573 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6184 136 Size: 107 122 71 631 605 4512 1525 Fragments arranged by size: 4512 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3051 7568 3051 Size: 4517 3056 BlpI GC'TnA_GC Cuts at: 4990 4990 Size: 7573 Bme1580I G_kGCm'C Cuts at: 1790 2772 4715 5634 6790 1790 Size: 982 1943 919 1156 2573 Fragments arranged by size: 2573 1943 1156 982 919 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7573 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2039 2502 2834 Size: 416 53 83 399 24 622 463 332 Cuts at: 2834 3427 5304 6262 442 Size: 593 1877 958 1753 Fragments arranged by size: 1877 1753 958 622 593 463 416 399 332 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3489 1056 Size: 2433 5140 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4057 4834 4057 Size: 777 6796 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2685 2810 3652 3705 3905 6233 1239 Size: 1446 125 842 53 200 2328 2579 Fragments arranged by size: 2579 2328 1446 842 200 125 53 Bpu10I CC'TnA_GC Cuts at: 2912 3156 3867 4011 4029 4065 4103 2912 Size: 244 711 144 18 36 38 6382 Fragments arranged by size: 6382 711 244 144 38 36 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2594 3032 5698 6563 6861 2594 Size: 438 2666 865 298 3306 Fragments arranged by size: 3306 2666 865 438 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2090 4010 4114 4667 6236 882 Size: 617 591 1920 104 553 1569 2219 Fragments arranged by size: 2219 1920 1569 617 591 553 104 BsaAI yAC'GTr Cuts at: 493 1204 5364 493 Size: 711 4160 2702 Fragments arranged by size: 4160 2702 711 BsaBI GATnn'nnATC Cuts at: 7567 7567 Size: 7573 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4855 5380 5762 6397 Size: 53 83 186 936 3322 525 382 635 Cuts at: 6397 275 Size: 1451 Fragments arranged by size: 3322 1451 936 635 525 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6006 6404 6678 6825 7236 6006 Size: 398 274 147 411 6343 Fragments arranged by size: 6343 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2470 2500 2539 2569 3529 Size: 30 165 30 1067 30 39 30 960 Cuts at: 3529 3559 4373 4403 1178 Size: 30 814 30 4348 Fragments arranged by size: 4348 1067 960 814 165 39 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3116 3431 4028 4046 4070 4685 1186 Size: 1930 315 597 18 24 615 4074 Fragments arranged by size: 4074 1930 615 597 315 24 18 BseYI C'CCAG_C Cuts at: 2085 2115 2171 3455 4139 4240 4323 4632 6776 Size: 30 56 1284 684 101 83 309 2144 Cuts at: 6776 7272 2085 Size: 496 2386 Fragments arranged by size: 2386 2144 1284 684 496 309 101 83 56 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2365 1163 Size: 573 629 6371 Fragments arranged by size: 6371 629 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5023 5784 6812 1540 Size: 275 186 3022 761 1028 2301 Fragments arranged by size: 3022 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2216 2304 2480 3006 4551 4631 4731 5634 5719 Size: 88 176 526 1545 80 100 903 85 Cuts at: 5719 6790 2216 Size: 1071 2999 Fragments arranged by size: 2999 1545 1071 903 526 176 100 88 85 80 BsmI GAATG_Cn' Cuts at: 2919 3765 4012 4042 5084 5177 2919 Size: 846 247 30 1042 93 5315 Fragments arranged by size: 5315 1042 846 247 93 30 BsmBI CGTCTCn'nnnn_ Cuts at: 3985 3985 Size: 7573 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2216 2304 2480 2508 2772 3006 3668 Size: 399 426 88 176 28 264 234 662 Cuts at: 3668 3865 3883 4551 4631 4715 4731 4800 4827 Size: 197 18 668 80 84 16 69 27 Cuts at: 4827 4892 5634 5719 6790 1391 Size: 65 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 668 662 426 399 264 234 197 176 88 85 84 80 69 65 28 27 18 16 BspEI T'CCGG_A Cuts at: 7236 7236 Size: 7573 BspHI T'CATG_A Cuts at: 2562 3511 5462 7192 2562 Size: 949 1951 1730 2943 Fragments arranged by size: 2943 1951 1730 949 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3770 4440 4605 4627 4928 844 Size: 2926 670 165 22 301 3489 Fragments arranged by size: 3489 2926 670 301 165 22 BsrBI CCG'CTC Cuts at: 1394 1823 3230 5460 1394 Size: 429 1407 2230 3507 Fragments arranged by size: 3507 2230 1407 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3211 4527 6068 6242 65 Size: 1183 1963 1316 1541 174 1396 Fragments arranged by size: 1963 1541 1396 1316 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6217 6404 1700 Size: 110 4407 187 2869 Fragments arranged by size: 4407 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7573 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7573 BssSI C'ACGA_G Cuts at: 5633 6645 5633 Size: 1012 6561 BstAPI GCAn_nnn'nTGC Cuts at: 794 2125 2144 2343 4339 794 Size: 1331 19 199 1996 4028 Fragments arranged by size: 4028 1996 1331 199 19 BstBI TT'CG_AA Cuts at: 6377 6377 Size: 7573 BstEII G'GTnAC_C Cuts at: 1184 3022 3757 1184 Size: 1838 735 5000 Fragments arranged by size: 5000 1838 735 BstXI CCAn_nnnn'nTGG Cuts at: 4684 4715 4684 Size: 31 7542 BstYI r'GATC_y Cuts at: 1972 2122 2258 3051 4903 5270 5655 5672 7124 Size: 150 136 793 1852 367 385 17 1452 Cuts at: 7124 7210 7222 7568 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1852 1452 793 385 367 346 150 136 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2367 3099 3753 4199 4257 4809 2367 Size: 732 654 446 58 552 5131 Fragments arranged by size: 5131 732 654 552 446 58 BtgI C'CryG_G Cuts at: 513 1065 2004 2877 2940 4415 4683 513 Size: 552 939 873 63 1475 268 3403 Fragments arranged by size: 3403 1475 939 873 552 268 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3689 487 Size: 683 56 2463 4371 Fragments arranged by size: 4371 2463 683 56 BtsI GCAGTG_nn' Cuts at: 2158 2344 2870 3646 4652 4705 5089 5883 5903 Size: 186 526 776 1006 53 384 794 20 Cuts at: 5903 2158 Size: 3828 Fragments arranged by size: 3828 1006 794 776 526 384 186 53 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7573 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7538 DraI TTT'AAA Cuts at: 4877 4877 Size: 7573 DraIII CAC_nnn'GTG Cuts at: 3247 3390 3247 Size: 143 7430 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6580 809 Size: 507 5264 1802 Fragments arranged by size: 5264 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2196 2430 Size: 54 1425 50 275 18 177 189 234 Cuts at: 2430 2880 3300 4092 4367 4418 4468 5020 5909 Size: 450 420 792 275 51 50 552 889 Cuts at: 5909 7538 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 792 552 450 420 275 275 234 189 177 54 51 50 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5020 1537 Size: 275 3208 4090 Fragments arranged by size: 4090 3208 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3237 3915 4047 4077 4584 5503 1353 Size: 1884 678 132 30 507 919 3423 Fragments arranged by size: 3423 1884 919 678 507 132 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6160 6534 6680 387 Size: 1064 138 4571 374 146 1280 Fragments arranged by size: 4571 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3004 4549 4629 4729 3004 Size: 1545 80 100 5848 Fragments arranged by size: 5848 1545 100 80 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2127 2535 2607 2685 2810 2981 3095 Size: 393 495 408 72 78 125 171 114 Cuts at: 3095 3652 3705 3905 4211 4230 4481 5636 6233 Size: 557 53 200 306 19 251 1155 597 Cuts at: 6233 7020 7288 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1155 787 597 557 495 408 393 306 268 251 200 171 125 114 78 72 53 19 EcoO109I rG'GnC_Cy Cuts at: 2111 2769 2887 3748 4594 4711 4712 2111 Size: 658 118 861 846 117 1 4972 Fragments arranged by size: 4972 861 846 658 118 117 1 EcoRI G'AATT_C Cuts at: 4882 5260 4882 Size: 378 7195 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2986 3494 2986 Size: 508 7065 FspI TGC'GCA Cuts at: 6079 6079 Size: 7573 HaeII r_GCGC'y Cuts at: 1678 6720 1678 Size: 5042 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2243 2275 3016 3048 3267 Size: 32 135 32 671 32 741 32 219 Cuts at: 3267 3299 3529 3561 4253 4285 4373 4405 4845 Size: 32 230 32 692 32 88 32 440 Cuts at: 4845 4877 6214 6246 6288 6320 1373 Size: 32 1337 32 42 32 2626 Fragments arranged by size: 2626 1337 741 692 671 440 230 219 135 88 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2661 4917 5163 669 Size: 988 281 723 2256 246 3079 Fragments arranged by size: 3079 2256 988 723 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7573 HpaI GTT'AAC Cuts at: 5163 5163 Size: 7573 KpnI G_GTAC'C Cuts at: 4898 4898 Size: 7573 MfeI C'AATT_G Cuts at: 5172 5172 Size: 7573 MluI A'CGCG_T Cuts at: 7449 7449 Size: 7573 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2948 3527 3794 6687 6871 1305 Size: 1643 579 267 2893 184 2007 Fragments arranged by size: 2893 2007 1643 579 267 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2198 2432 3302 4094 Size: 54 1425 343 177 189 234 870 792 Cuts at: 4094 4369 10 Size: 275 3214 Fragments arranged by size: 3214 1425 870 792 343 275 234 189 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3590 5531 5890 6049 518 Size: 648 2424 1941 359 159 2042 Fragments arranged by size: 2424 2042 1941 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2093 2801 3090 3410 3520 4330 4989 5666 Size: 487 708 289 320 110 810 659 677 Cuts at: 5666 6814 7059 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 810 708 677 659 487 320 289 245 110 NcoI C'CATG_G Cuts at: 513 1065 2004 2940 513 Size: 552 939 936 5146 Fragments arranged by size: 5146 939 936 552 NdeI CA'TA_TG Cuts at: 387 2289 387 Size: 1902 5671 NheI G'CTAG_C Cuts at: 1052 3485 1052 Size: 2433 5140 NotI GC'GGCC_GC Cuts at: 5020 5020 Size: 7573 NsiI A_TGCA'T Cuts at: 2039 2039 Size: 7573 NspI r_CATG'y Cuts at: 1132 1229 2397 2849 4931 6476 1132 Size: 97 1168 452 2082 1545 2229 Fragments arranged by size: 2229 2082 1545 1168 452 97 PasI CC'CwG_GG Cuts at: 1348 3560 1348 Size: 2212 5361 PciI A'CATG_T Cuts at: 2393 6472 2393 Size: 4079 3494 PflMI CCAn_nnn'nTGG Cuts at: 1108 2281 2635 4153 1108 Size: 1173 354 1518 4528 Fragments arranged by size: 4528 1518 1173 354 PmeI GTTT'AAAC Cuts at: 4877 4877 Size: 7573 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5642 5674 7155 7187 5642 Size: 32 1481 32 6028 Fragments arranged by size: 6028 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2111 3748 4594 2111 Size: 1637 846 5090 Fragments arranged by size: 5090 1637 846 PshAI GACnn'nnGTC Cuts at: 1613 3350 1613 Size: 1737 5836 PsiI TTA'TAA Cuts at: 5143 5143 Size: 7573 PspOMI G'GGCC_C Cuts at: 2768 4711 2768 Size: 1943 5630 PstI C_TGCA'G Cuts at: 830 4557 4602 4925 830 Size: 3727 45 323 3478 Fragments arranged by size: 3727 3478 323 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7573 PvuII CAG'CTG Cuts at: 2801 3090 3410 3520 2801 Size: 289 320 110 6854 Fragments arranged by size: 6854 320 289 110 SacI G_AGCT'C Cuts at: 3006 4551 4631 4731 3006 Size: 1545 80 100 5848 Fragments arranged by size: 5848 1545 100 80 SalI G'TCGA_C Cuts at: 1936 2659 4915 1936 Size: 723 2256 4594 Fragments arranged by size: 4594 2256 723 SapI GCTCTTCn'nnn_ Cuts at: 3237 3237 Size: 7573 SbfI CC_TGCA'GG Cuts at: 4557 4925 4557 Size: 368 7205 ScaI AGT'ACT Cuts at: 2179 5821 7533 2179 Size: 3642 1712 2219 Fragments arranged by size: 3642 2219 1712 SfcI C'TryA_G Cuts at: 826 1045 4553 4598 4921 6056 6737 6928 826 Size: 219 3508 45 323 1135 681 191 1471 Fragments arranged by size: 3508 1471 1135 681 323 219 191 45 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7573 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7573 SmaI CCC'GGG Cuts at: 1872 4900 1872 Size: 3028 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2609 3047 5677 6578 6840 820 Size: 197 926 666 438 2630 901 262 1553 Fragments arranged by size: 2630 1553 926 901 666 438 262 197 SnaBI TAC'GTA Cuts at: 493 5364 493 Size: 4871 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7573 SphI G_CATG'C Cuts at: 1132 2849 4931 1132 Size: 1717 2082 3774 Fragments arranged by size: 3774 2082 1717 SspI AAT'ATT Cuts at: 5 52 5497 5 Size: 47 5445 2081 Fragments arranged by size: 5445 2081 47 StuI AGG'CCT Cuts at: 1517 2645 1517 Size: 1128 6445 StyI C'CwwG_G Cuts at: 513 1065 2004 2883 2940 3744 5012 513 Size: 552 939 879 57 804 1268 3074 Fragments arranged by size: 3074 1268 939 879 804 552 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2331 2627 3391 4232 4742 5600 5617 Size: 1255 751 296 764 841 510 858 17 Cuts at: 5617 5770 5955 6400 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 858 841 764 751 510 445 296 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2177 5819 7531 Size: 274 80 33 51 1443 200 3642 1712 Cuts at: 7531 96 Size: 138 Fragments arranged by size: 3642 1712 1443 274 200 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2470 2502 4825 4857 7533 7565 2470 Size: 32 2323 32 2676 32 2478 Fragments arranged by size: 2676 2478 2323 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7573 XbaI T'CTAG_A Cuts at: 4909 4909 Size: 7573 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7573 XmaI C'CCGG_G Cuts at: 1870 4898 1870 Size: 3028 4545 XmnI GAAnn'nnTTC Cuts at: 2969 3554 3626 5702 2969 Size: 585 72 2076 4840 Fragments arranged by size: 4840 2076 585 72 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4856 5381 276 Size: 53 83 186 936 3322 525 2468 Fragments arranged by size: 3322 2468 936 525 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI