(Circular) (Six-base) MAPSORT of: pFN21AE1080.seq Check: 3061 from: 1 to: 8479 pFN21AE1080 8479 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2747 2747 Size: 8479 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6289 278 Size: 53 83 186 936 4753 2468 Fragments arranged by size: 4753 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3858 5822 1937 Size: 1921 1964 4594 Fragments arranged by size: 4594 1964 1921 Acc65I G'GTAC_C Cuts at: 5800 5800 Size: 8479 AclI AA'CG_TT Cuts at: 1563 5647 6606 6979 1563 Size: 4084 959 373 3063 Fragments arranged by size: 4084 3063 959 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2059 2696 3261 4335 5322 5694 6542 7926 Size: 427 637 565 1074 987 372 848 1384 Cuts at: 7926 8194 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1074 987 848 637 565 427 372 268 AflII C'TTAA_G Cuts at: 820 1017 5635 820 Size: 197 4618 3664 Fragments arranged by size: 4618 3664 197 AflIII A'CryG_T Cuts at: 1203 3578 3968 4375 5053 7378 8355 1203 Size: 2375 390 407 678 2325 977 1327 Fragments arranged by size: 2375 2325 1327 977 678 407 390 AgeI A'CCGG_T Cuts at: 7310 7310 Size: 8479 AhdI GACnn_n'nnGTC Cuts at: 7208 7208 Size: 8479 AleI CACnn'nnGTG Cuts at: 1166 3786 1166 Size: 2620 5859 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8479 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2966 2998 2966 Size: 32 8447 AlwNI CAG_nnn'CTG Cuts at: 2748 4991 5567 6222 7794 2748 Size: 2243 576 655 1572 3433 Fragments arranged by size: 3433 2243 1572 655 576 ApaLI G'TGCA_C Cuts at: 5530 6536 7692 5530 Size: 1006 1156 6317 Fragments arranged by size: 6317 1156 1006 ApoI r'AATT_y Cuts at: 1493 2348 2367 2918 3052 3121 3329 4444 4478 Size: 855 19 551 134 69 208 1115 34 Cuts at: 4478 5036 5185 5337 5472 5788 6014 6166 8275 Size: 558 149 152 135 316 226 152 2109 Cuts at: 8275 1493 Size: 1697 Fragments arranged by size: 2109 1697 1115 855 558 551 316 226 208 152 152 149 135 134 69 34 19 AseI AT'TA_AT Cuts at: 160 1032 3251 7033 160 Size: 872 2219 3782 1606 Fragments arranged by size: 3782 2219 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8479 AvaI C'yCGr_G Cuts at: 1870 1943 3381 5804 1870 Size: 73 1438 2423 4545 Fragments arranged by size: 4545 2423 1438 73 AvrII C'CTAG_G Cuts at: 2549 4214 2549 Size: 1665 6814 BamHI G'GATC_C Cuts at: 5809 6176 5809 Size: 367 8112 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3211 5800 7255 618 Size: 325 217 627 1424 2589 1455 1842 Fragments arranged by size: 2589 1842 1455 1424 627 325 217 BanII G_rGCy'C Cuts at: 1391 4390 5798 1391 Size: 2999 1408 4072 Fragments arranged by size: 4072 2999 1408 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2570 4113 4359 928 Size: 971 671 1543 246 5048 Fragments arranged by size: 5048 1543 971 671 246 BbvCI CC'TCA_GC Cuts at: 2664 5752 2664 Size: 3088 5391 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2606 2626 2640 2660 6670 6704 1903 Size: 34 669 20 14 20 4010 34 3678 Fragments arranged by size: 4010 3678 669 34 34 20 20 14 BciVI GTATCCnnnnn_n' Cuts at: 3824 6371 7581 3824 Size: 2547 1210 4722 Fragments arranged by size: 4722 2547 1210 BclI T'GATC_A Cuts at: 1548 3931 4936 1548 Size: 2383 1005 5091 Fragments arranged by size: 5091 2383 1005 BfrBI ATG'CAT Cuts at: 2237 4781 4808 2237 Size: 2544 27 5908 Fragments arranged by size: 5908 2544 27 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7090 136 Size: 107 122 71 631 605 5418 1525 Fragments arranged by size: 5418 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8474 8474 Size: 8479 BlpI GC'TnA_GC Cuts at: 5896 5896 Size: 8479 Bme1580I G_kGCm'C Cuts at: 1790 5534 5676 6540 7696 1790 Size: 3744 142 864 1156 2573 Fragments arranged by size: 3744 2573 1156 864 142 BmgBI CAC'GTC Cuts at: 1318 2499 4357 1318 Size: 1181 1858 5440 Fragments arranged by size: 5440 1858 1181 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4296 6210 7168 Size: 416 53 83 399 24 2879 1914 958 Cuts at: 7168 442 Size: 1753 Fragments arranged by size: 2879 1914 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8479 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2362 4820 7139 1239 Size: 1123 2458 2319 2579 Fragments arranged by size: 2579 2458 2319 1123 Bpu10I CC'TnA_GC Cuts at: 2664 5752 2664 Size: 3088 5391 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4403 6604 7469 7767 4403 Size: 2201 865 298 5115 Fragments arranged by size: 5115 2201 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7142 882 Size: 617 5643 2219 Fragments arranged by size: 5643 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3971 6270 493 Size: 711 2767 2299 2702 Fragments arranged by size: 2767 2702 2299 711 BsaBI GATnn'nnATC Cuts at: 5145 8473 5145 Size: 3328 5151 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4305 6286 6668 7303 Size: 53 83 186 936 2772 1981 382 635 Cuts at: 7303 275 Size: 1451 Fragments arranged by size: 2772 1981 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6912 7310 7584 7731 8142 6912 Size: 398 274 147 411 7249 Fragments arranged by size: 7249 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3936 3966 5062 5092 1178 Size: 30 165 30 2533 30 1096 30 4565 Fragments arranged by size: 4565 2533 1096 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2681 4190 4193 4211 4214 5395 1186 Size: 1495 1509 3 18 3 1181 4270 Fragments arranged by size: 4270 1509 1495 1181 18 3 3 BseYI C'CCAG_C Cuts at: 5766 7682 8178 5766 Size: 1916 496 6067 Fragments arranged by size: 6067 1916 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2610 1163 Size: 573 874 7032 Fragments arranged by size: 7032 874 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5929 6690 7718 1540 Size: 275 186 3928 761 1028 2301 Fragments arranged by size: 3928 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3992 4390 5534 6540 6625 7696 3992 Size: 398 1144 1006 85 1071 4775 Fragments arranged by size: 4775 1144 1071 1006 398 85 BsmI GAATG_Cn' Cuts at: 3574 5693 5990 6083 3574 Size: 2119 297 93 5970 Fragments arranged by size: 5970 2119 297 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3992 4390 5534 5676 5798 6540 6625 Size: 399 2202 398 1144 142 122 742 85 Cuts at: 6625 7696 1391 Size: 1071 2174 Fragments arranged by size: 2202 2174 1144 1071 742 399 398 142 122 85 BspEI T'CCGG_A Cuts at: 8142 8142 Size: 8479 BspHI T'CATG_A Cuts at: 5361 6368 8098 5361 Size: 1007 1730 5742 Fragments arranged by size: 5742 1730 1007 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2747 5834 844 Size: 1903 3087 3489 Fragments arranged by size: 3489 3087 1903 BsrBI CCG'CTC Cuts at: 1394 1823 6366 1394 Size: 429 4543 3507 Fragments arranged by size: 4543 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2769 2892 3411 6974 7148 65 Size: 1183 1521 123 519 3563 174 1396 Fragments arranged by size: 3563 1521 1396 1183 519 174 123 BsrFI r'CCGG_y Cuts at: 1700 1810 7123 7310 1700 Size: 110 5313 187 2869 Fragments arranged by size: 5313 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3625 96 Size: 3529 4950 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8479 BssSI C'ACGA_G Cuts at: 6539 7551 6539 Size: 1012 7467 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8479 BstBI TT'CG_AA Cuts at: 7283 7283 Size: 8479 BstEII G'GTnAC_C Cuts at: 1184 2084 1184 Size: 900 7579 BstXI CCAn_nnnn'nTGG Cuts at: 3674 3674 Size: 8479 BstYI r'GATC_y Cuts at: 1972 3424 5131 5586 5809 6176 6561 6578 8030 Size: 1452 1707 455 223 367 385 17 1452 Cuts at: 8030 8116 8128 8474 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1707 1452 1452 455 385 367 346 223 86 17 12 BstZ17I GTA'TAC Cuts at: 3859 3859 Size: 8479 Bsu36I CC'TnA_GG Cuts at: 5591 5591 Size: 8479 BtgI C'CryG_G Cuts at: 513 1065 2004 2131 2558 513 Size: 552 939 127 427 6434 Fragments arranged by size: 6434 939 552 427 127 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7740 Fragments arranged by size: 7740 683 56 BtsI GCAGTG_nn' Cuts at: 5995 6789 6809 5995 Size: 794 20 7665 Fragments arranged by size: 7665 794 20 ClaI AT'CG_AT Cuts at: 1554 2616 1554 Size: 1062 7417 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8444 DraI TTT'AAA Cuts at: 2242 5047 5701 5783 2242 Size: 2805 654 82 4938 Fragments arranged by size: 4938 2805 654 82 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7486 809 Size: 507 6170 1802 Fragments arranged by size: 6170 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5926 6815 8444 Size: 54 1425 50 275 18 4096 889 1629 Cuts at: 8444 8 Size: 43 Fragments arranged by size: 4096 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5926 1537 Size: 275 4114 4090 Fragments arranged by size: 4114 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2672 2832 3201 3531 4167 4188 4311 4884 Size: 1319 160 369 330 636 21 123 573 Cuts at: 4884 5212 5609 5659 5713 5753 6409 1353 Size: 328 397 50 54 40 656 3423 Fragments arranged by size: 3423 1319 656 636 573 397 369 330 328 160 123 54 50 40 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4696 7066 7440 7586 387 Size: 1064 138 3107 2370 374 146 1280 Fragments arranged by size: 3107 2370 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4388 4388 Size: 8479 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2059 2362 2696 3261 4335 4820 5322 Size: 393 427 303 334 565 1074 485 502 Cuts at: 5322 5694 6542 7139 7926 8194 1239 Size: 372 848 597 787 268 1524 Fragments arranged by size: 1524 1074 848 787 597 565 502 485 427 393 372 334 303 268 EcoO109I rG'GnC_Cy Cuts at: 2152 2606 3671 5763 2152 Size: 454 1065 2092 4868 Fragments arranged by size: 4868 2092 1065 454 EcoRI G'AATT_C Cuts at: 2348 2367 4478 5788 6166 2348 Size: 19 2111 1310 378 4661 Fragments arranged by size: 4661 2111 1310 378 19 EcoRV GAT'ATC Cuts at: 4651 4651 Size: 8479 FspI TGC'GCA Cuts at: 6985 6985 Size: 8479 HaeII r_GCGC'y Cuts at: 1678 7626 1678 Size: 5948 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3416 3448 3815 3847 3916 Size: 32 135 32 1844 32 367 32 69 Cuts at: 3916 3948 4295 4327 4621 4653 5062 5094 5246 Size: 32 347 32 294 32 409 32 152 Cuts at: 5246 5278 7120 7152 7194 7226 1373 Size: 32 1842 32 42 32 2626 Fragments arranged by size: 2626 1844 1842 409 367 347 294 152 135 69 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2261 3470 3829 3924 5298 5505 Size: 988 281 323 1209 359 95 1374 207 Cuts at: 5505 5823 6069 669 Size: 318 246 3079 Fragments arranged by size: 3079 1374 1209 988 359 323 318 281 246 207 95 HindIII A'AGCT_T Cuts at: 748 748 Size: 8479 HpaI GTT'AAC Cuts at: 3829 5298 6069 3829 Size: 1469 771 6239 Fragments arranged by size: 6239 1469 771 KpnI G_GTAC'C Cuts at: 5804 5804 Size: 8479 MfeI C'AATT_G Cuts at: 4608 6078 4608 Size: 1470 7009 MluI A'CGCG_T Cuts at: 8355 8355 Size: 8479 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3010 3880 7593 7777 1305 Size: 1705 870 3713 184 2007 Fragments arranged by size: 3713 2007 1705 870 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6657 Fragments arranged by size: 6657 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3621 3786 6437 6796 6955 518 Size: 648 2455 165 2651 359 159 2042 Fragments arranged by size: 2651 2455 2042 648 359 165 159 MspA1I CmG'CkG Cuts at: 1606 3692 5564 5895 6572 7720 7965 1606 Size: 2086 1872 331 677 1148 245 2120 Fragments arranged by size: 2120 2086 1872 1148 677 331 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2131 513 Size: 552 939 127 6861 Fragments arranged by size: 6861 939 552 127 NdeI CA'TA_TG Cuts at: 387 2233 387 Size: 1846 6633 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8479 NotI GC'GGCC_GC Cuts at: 5926 5926 Size: 8479 NsiI A_TGCA'T Cuts at: 2239 4783 4810 2239 Size: 2544 27 5908 Fragments arranged by size: 5908 2544 27 NspI r_CATG'y Cuts at: 1132 1229 3582 3953 4379 5057 5837 7382 1132 Size: 97 2353 371 426 678 780 1545 2229 Fragments arranged by size: 2353 2229 1545 780 678 426 371 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8479 PciI A'CATG_T Cuts at: 3578 4375 5053 7378 3578 Size: 797 678 2325 4679 Fragments arranged by size: 4679 2325 797 678 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8479 PmeI GTTT'AAAC Cuts at: 5783 5783 Size: 8479 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4203 4235 6548 6580 8061 8093 4203 Size: 32 2313 32 1481 32 4589 Fragments arranged by size: 4589 2313 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3671 5763 3671 Size: 2092 6387 PshAI GACnn'nnGTC Cuts at: 1613 3244 1613 Size: 1631 6848 PsiI TTA'TAA Cuts at: 2378 5192 6049 2378 Size: 2814 857 4808 Fragments arranged by size: 4808 2814 857 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5414 5446 5414 Size: 32 8447 PstI C_TGCA'G Cuts at: 830 2450 2744 3184 5831 830 Size: 1620 294 440 2647 3478 Fragments arranged by size: 3478 2647 1620 440 294 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8479 PvuII CAG'CTG Cuts at: 3692 5564 3692 Size: 1872 6607 SacI G_AGCT'C Cuts at: 4390 4390 Size: 8479 SalI G'TCGA_C Cuts at: 1936 5821 1936 Size: 3885 4594 SapI GCTCTTCn'nnn_ Cuts at: 3531 5713 3531 Size: 2182 6297 SbfI CC_TGCA'GG Cuts at: 5831 5831 Size: 8479 ScaI AGT'ACT Cuts at: 6727 8439 6727 Size: 1712 6767 SfcI C'TryA_G Cuts at: 826 1045 2254 2446 2724 2740 3031 3180 5827 Size: 219 1209 192 278 16 291 149 2647 Cuts at: 5827 6962 7643 7834 826 Size: 1135 681 191 1471 Fragments arranged by size: 2647 1471 1209 1135 681 291 278 219 192 191 149 16 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8479 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8479 SmaI CCC'GGG Cuts at: 1872 5806 1872 Size: 3934 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4418 5635 6583 7484 7746 820 Size: 197 926 2475 1217 948 901 262 1553 Fragments arranged by size: 2475 1553 1217 948 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6270 493 Size: 5777 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8479 SphI G_CATG'C Cuts at: 1132 5837 1132 Size: 4705 3774 SspI AAT'ATT Cuts at: 5 52 3255 4905 4924 6403 5 Size: 47 3203 1650 19 1479 2081 Fragments arranged by size: 3203 2081 1650 1479 47 19 StuI AGG'CCT Cuts at: 1517 1517 Size: 8479 StyI C'CwwG_G Cuts at: 513 1065 2004 2131 2549 3667 4214 5918 513 Size: 552 939 127 418 1118 547 1704 3074 Fragments arranged by size: 3074 1704 1118 939 552 547 418 127 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3289 6506 6523 6676 6861 7306 325 Size: 1255 1709 3217 17 153 185 445 1498 Fragments arranged by size: 3217 1709 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2733 3625 4082 Size: 274 80 33 51 1443 756 892 457 Cuts at: 4082 4135 5484 6725 8437 96 Size: 53 1349 1241 1712 138 Fragments arranged by size: 1712 1443 1349 1241 892 756 457 274 138 80 53 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8439 8471 8439 Size: 32 8447 Tth111I GACn'n_nGTC Cuts at: 1316 4520 1316 Size: 3204 5275 XbaI T'CTAG_A Cuts at: 4784 5815 4784 Size: 1031 7448 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2503 2905 4631 5607 2503 Size: 402 1726 976 5375 Fragments arranged by size: 5375 1726 976 402 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8479 XmaI C'CCGG_G Cuts at: 1870 5804 1870 Size: 3934 4545 XmnI GAAnn'nnTTC Cuts at: 4158 4561 4619 6608 4158 Size: 403 58 1989 6029 Fragments arranged by size: 6029 1989 403 58 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6287 276 Size: 53 83 186 936 4753 2468 Fragments arranged by size: 4753 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbeI BplI BsiWI BsmBI DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI