(Circular) (Six-base) MAPSORT of: pFN21AB7868.seq Check: 627 from: 1 to: 6607 pFN21AB7868 6607 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4417 278 Size: 53 83 186 936 2881 2468 Fragments arranged by size: 2881 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2898 3950 1937 Size: 961 1052 4594 Fragments arranged by size: 4594 1052 961 Acc65I G'GTAC_C Cuts at: 3928 3928 Size: 6607 AclI AA'CG_TT Cuts at: 1563 3906 4734 5107 1563 Size: 2343 828 373 3063 Fragments arranged by size: 3063 2343 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2614 4670 6054 6322 1632 Size: 982 2056 1384 268 1917 Fragments arranged by size: 2056 1917 1384 982 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6410 AflIII A'CryG_T Cuts at: 1203 2101 3841 5506 6483 1203 Size: 898 1740 1665 977 1327 Fragments arranged by size: 1740 1665 1327 977 898 AgeI A'CCGG_T Cuts at: 5438 5438 Size: 6607 AhdI GACnn_n'nnGTC Cuts at: 5336 5336 Size: 6607 AleI CACnn'nnGTG Cuts at: 1166 3383 1166 Size: 2217 4390 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6607 AlwNI CAG_nnn'CTG Cuts at: 2090 2158 2687 2738 3283 4350 5922 2090 Size: 68 529 51 545 1067 1572 2775 Fragments arranged by size: 2775 1572 1067 545 529 68 51 ApaLI G'TGCA_C Cuts at: 2431 4664 5820 2431 Size: 2233 1156 3218 Fragments arranged by size: 3218 2233 1156 ApoI r'AATT_y Cuts at: 1493 3916 4142 4294 6403 1493 Size: 2423 226 152 2109 1697 Fragments arranged by size: 2423 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5161 160 Size: 872 4129 1606 Fragments arranged by size: 4129 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6607 AvaI C'yCGr_G Cuts at: 1870 1943 3932 1870 Size: 73 1989 4545 Fragments arranged by size: 4545 1989 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2090 2123 2090 Size: 33 6574 BamHI G'GATC_C Cuts at: 3937 4304 3937 Size: 367 6240 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2312 2521 3702 3928 5383 Size: 325 217 627 525 209 1181 226 1455 Cuts at: 5383 618 Size: 1842 Fragments arranged by size: 1842 1455 1181 627 525 325 226 217 209 BanII G_rGCy'C Cuts at: 1391 2215 2567 2847 3105 3926 1391 Size: 824 352 280 258 821 4072 Fragments arranged by size: 4072 824 821 352 280 258 BbeI G_GCGC'C Cuts at: 2316 2316 Size: 6607 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3280 3294 3608 928 Size: 971 1381 14 314 3927 Fragments arranged by size: 3927 1381 971 314 14 BbvCI CC'TCA_GC Cuts at: 2354 2461 3071 2354 Size: 107 610 5890 Fragments arranged by size: 5890 610 107 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3797 3831 4798 4832 1903 Size: 34 1860 34 967 34 3678 Fragments arranged by size: 3678 1860 967 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4499 5709 4499 Size: 1210 5397 BclI T'GATC_A Cuts at: 1548 2254 1548 Size: 706 5901 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5218 136 Size: 107 122 71 631 605 3546 1525 Fragments arranged by size: 3546 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2956 2983 3456 6602 2956 Size: 27 473 3146 2961 Fragments arranged by size: 3146 2961 473 27 BlpI GC'TnA_GC Cuts at: 4024 4024 Size: 6607 Bme1580I G_kGCm'C Cuts at: 1790 2131 2435 2543 3380 4668 5824 1790 Size: 341 304 108 837 1288 1156 2573 Fragments arranged by size: 2573 1288 1156 837 341 304 108 BmgBI CAC'GTC Cuts at: 1318 3381 3842 1318 Size: 2063 461 4083 Fragments arranged by size: 4083 2063 461 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2022 2289 2902 Size: 416 53 83 399 24 605 267 613 Cuts at: 2902 4338 5296 442 Size: 1436 958 1753 Fragments arranged by size: 1753 1436 958 613 605 416 399 267 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6607 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3795 3795 Size: 6607 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2569 2801 2981 3019 3447 5267 1239 Size: 1330 232 180 38 428 1820 2579 Fragments arranged by size: 2579 1820 1330 428 232 180 38 Bpu10I CC'TnA_GC Cuts at: 2354 2461 3071 2354 Size: 107 610 5890 Fragments arranged by size: 5890 610 107 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3602 4732 5597 5895 3602 Size: 1130 865 298 4314 Fragments arranged by size: 4314 1130 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2845 3620 5270 882 Size: 617 1346 775 1650 2219 Fragments arranged by size: 2219 1650 1346 775 617 BsaAI yAC'GTr Cuts at: 493 1204 2902 3514 4398 493 Size: 711 1698 612 884 2702 Fragments arranged by size: 2702 1698 884 711 612 BsaBI GATnn'nnATC Cuts at: 6601 6601 Size: 6607 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2313 4414 4796 5431 Size: 53 83 186 936 780 2101 382 635 Cuts at: 5431 275 Size: 1451 Fragments arranged by size: 2101 1451 936 780 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2847 5040 5438 5712 5859 6270 2847 Size: 2193 398 274 147 411 3184 Fragments arranged by size: 3184 2193 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3382 3412 3891 3921 1178 Size: 30 165 30 1979 30 479 30 3864 Fragments arranged by size: 3864 1979 479 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2007 2227 2727 2978 2987 2990 3134 3231 Size: 821 220 500 251 9 3 144 97 Cuts at: 3231 3912 1186 Size: 681 3881 Fragments arranged by size: 3881 821 681 500 251 220 144 97 9 3 BseYI C'CCAG_C Cuts at: 2516 2612 2731 2840 2923 5810 6306 2516 Size: 96 119 109 83 2887 496 2817 Fragments arranged by size: 2887 2817 496 119 109 96 83 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2213 1163 Size: 573 477 5557 Fragments arranged by size: 5557 573 477 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3248 3539 4057 4818 5846 1540 Size: 275 186 1247 291 518 761 1028 2301 Fragments arranged by size: 2301 1247 1028 761 518 291 275 186 BsiHKAI G_wGCw'C Cuts at: 2406 2435 3597 4668 4753 5824 2406 Size: 29 1162 1071 85 1071 3189 Fragments arranged by size: 3189 1162 1071 1071 85 29 BsmI GAATG_Cn' Cuts at: 4118 4211 4118 Size: 93 6514 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2131 2215 2406 2435 2543 2567 2847 Size: 399 341 84 191 29 108 24 280 Cuts at: 2847 3105 3380 3597 3926 4668 4753 5824 1391 Size: 258 275 217 329 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 341 329 280 275 258 217 191 108 85 84 29 24 BspEI T'CCGG_A Cuts at: 2847 6270 2847 Size: 3423 3184 BspHI T'CATG_A Cuts at: 4496 6226 4496 Size: 1730 4877 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3115 3176 3962 844 Size: 2271 61 786 3489 Fragments arranged by size: 3489 2271 786 61 BsrBI CCG'CTC Cuts at: 1394 1823 3250 4494 1394 Size: 429 1427 1244 3507 Fragments arranged by size: 3507 1427 1244 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5102 5276 65 Size: 1183 3854 174 1396 Fragments arranged by size: 3854 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3507 3699 5251 5438 1700 Size: 110 1697 192 1552 187 2869 Fragments arranged by size: 2869 1697 1552 192 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6607 BssHII G'CGCG_C Cuts at: 1925 2570 1925 Size: 645 5962 BssSI C'ACGA_G Cuts at: 4667 5679 4667 Size: 1012 5595 BstAPI GCAn_nnn'nTGC Cuts at: 794 2158 2687 794 Size: 1364 529 4714 Fragments arranged by size: 4714 1364 529 BstBI TT'CG_AA Cuts at: 5411 5411 Size: 6607 BstEII G'GTnAC_C Cuts at: 1184 2119 1184 Size: 935 5672 BstXI CCAn_nnnn'nTGG Cuts at: 2302 2302 Size: 6607 BstYI r'GATC_y Cuts at: 1972 2956 2983 3279 3456 3937 4304 4689 4706 Size: 984 27 296 177 481 367 385 17 Cuts at: 4706 6158 6244 6256 6602 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 984 481 385 367 346 296 177 86 27 17 12 BstZ17I GTA'TAC Cuts at: 2899 2899 Size: 6607 Bsu36I CC'TnA_GG Cuts at: 2115 3060 2115 Size: 945 5662 BtgI C'CryG_G Cuts at: 513 1065 2004 2559 3369 3747 513 Size: 552 939 555 810 378 3373 Fragments arranged by size: 3373 939 810 555 552 378 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2190 2814 487 Size: 683 56 964 624 4280 Fragments arranged by size: 4280 964 683 624 56 BtsI GCAGTG_nn' Cuts at: 2100 2697 2704 2755 2832 3369 3445 4123 4917 Size: 597 7 51 77 537 76 678 794 Cuts at: 4917 4937 2100 Size: 20 3770 Fragments arranged by size: 3770 794 678 597 537 77 76 51 20 7 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6607 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6572 DraI TTT'AAA Cuts at: 3911 3911 Size: 6607 DraIII CAC_nnn'GTG Cuts at: 2422 3871 2422 Size: 1449 5158 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5614 809 Size: 507 4298 1802 Fragments arranged by size: 4298 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2347 3328 3536 Size: 54 1425 50 275 18 517 981 208 Cuts at: 3536 3819 4054 4943 6572 8 Size: 283 235 889 1629 43 Fragments arranged by size: 1629 1425 981 889 517 283 275 235 208 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3536 4054 1537 Size: 275 1724 518 4090 Fragments arranged by size: 4090 1724 518 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2964 3223 4537 1353 Size: 1611 259 1314 3423 Fragments arranged by size: 3423 1611 1314 259 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2055 5194 5568 5714 387 Size: 1064 138 466 3139 374 146 1280 Fragments arranged by size: 3139 1280 1064 466 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2569 2614 2801 2981 3019 3447 4670 Size: 393 937 45 187 180 38 428 1223 Cuts at: 4670 5267 6054 6322 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1223 937 787 597 428 393 268 187 180 45 38 EcoNI CCTnn'n_nnAGG Cuts at: 3183 3183 Size: 6607 EcoO109I rG'GnC_Cy Cuts at: 2260 3056 3176 3421 2260 Size: 796 120 245 5446 Fragments arranged by size: 5446 796 245 120 EcoRI G'AATT_C Cuts at: 3916 4294 3916 Size: 378 6229 FspI TGC'GCA Cuts at: 5113 5113 Size: 6607 HaeII r_GCGC'y Cuts at: 1678 2316 2930 3700 5754 1678 Size: 638 614 770 2054 2531 Fragments arranged by size: 2531 2054 770 638 614 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2591 2623 2642 2674 5248 Size: 32 135 32 1019 32 19 32 2574 Cuts at: 5248 5280 5322 5354 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2574 1019 135 42 32 32 32 32 32 32 19 HincII GTy'rAC Cuts at: 669 1657 1938 2380 2994 3130 3951 4197 669 Size: 988 281 442 614 136 821 246 3079 Fragments arranged by size: 3079 988 821 614 442 281 246 136 HindIII A'AGCT_T Cuts at: 748 748 Size: 6607 HpaI GTT'AAC Cuts at: 4197 4197 Size: 6607 KasI G'GCGC_C Cuts at: 2312 2312 Size: 6607 KpnI G_GTAC'C Cuts at: 3932 3932 Size: 6607 MfeI C'AATT_G Cuts at: 4206 4206 Size: 6607 MluI A'CGCG_T Cuts at: 6483 6483 Size: 6607 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5721 5905 1305 Size: 4416 184 2007 Fragments arranged by size: 4416 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3330 10 Size: 54 1425 343 1498 3287 Fragments arranged by size: 3287 1498 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3383 4565 4924 5083 518 Size: 648 2217 1182 359 159 2042 Fragments arranged by size: 2217 2042 1182 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2053 2090 3801 4023 4700 5848 6093 1606 Size: 447 37 1711 222 677 1148 245 2120 Fragments arranged by size: 2120 1711 1148 677 447 245 222 37 NaeI GCC'GGC Cuts at: 3701 3701 Size: 6607 NarI GG'CG_CC Cuts at: 2313 2313 Size: 6607 NcoI C'CATG_G Cuts at: 513 1065 2004 2559 513 Size: 552 939 555 4561 Fragments arranged by size: 4561 939 555 552 NdeI CA'TA_TG Cuts at: 387 3163 387 Size: 2776 3831 NgoMIV G'CCGG_C Cuts at: 3699 3699 Size: 6607 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6607 NotI GC'GGCC_GC Cuts at: 4054 4054 Size: 6607 NspI r_CATG'y Cuts at: 1132 1229 2105 3268 3502 3523 3965 5510 1132 Size: 97 876 1163 234 21 442 1545 2229 Fragments arranged by size: 2229 1545 1163 876 442 234 97 21 PasI CC'CwG_GG Cuts at: 1348 2637 1348 Size: 1289 5318 PciI A'CATG_T Cuts at: 2101 5506 2101 Size: 3405 3202 PflMI CCAn_nnn'nTGG Cuts at: 1108 3283 3871 1108 Size: 2175 588 3844 Fragments arranged by size: 3844 2175 588 PfoI T'CCnGG_A Cuts at: 2528 3781 2528 Size: 1253 5354 PmeI GTTT'AAAC Cuts at: 3911 3911 Size: 6607 PmlI CAC'GTG Cuts at: 3514 3514 Size: 6607 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4676 4708 6189 6221 4676 Size: 32 1481 32 5062 Fragments arranged by size: 5062 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2260 3176 3421 2260 Size: 916 245 5446 Fragments arranged by size: 5446 916 245 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6607 PsiI TTA'TAA Cuts at: 4177 4177 Size: 6607 PstI C_TGCA'G Cuts at: 830 2095 2163 2491 2692 3959 830 Size: 1265 68 328 201 1267 3478 Fragments arranged by size: 3478 1267 1265 328 201 68 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6607 PvuII CAG'CTG Cuts at: 2090 2090 Size: 6607 SalI G'TCGA_C Cuts at: 1936 3949 1936 Size: 2013 4594 SanDI GG'GwC_CC Cuts at: 3421 3421 Size: 6607 SbfI CC_TGCA'GG Cuts at: 3959 3959 Size: 6607 ScaI AGT'ACT Cuts at: 4855 6567 4855 Size: 1712 4895 SfcI C'TryA_G Cuts at: 826 1045 2091 2159 2487 2688 3955 5090 5771 Size: 219 1046 68 328 201 1267 1135 681 Cuts at: 5771 5962 826 Size: 191 1471 Fragments arranged by size: 1471 1267 1135 1046 681 328 219 201 191 68 SfoI GGC'GCC Cuts at: 2314 2314 Size: 6607 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6607 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6607 SmaI CCC'GGG Cuts at: 1872 3934 1872 Size: 2062 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3617 4711 5612 5874 820 Size: 197 926 1674 1094 901 262 1553 Fragments arranged by size: 1674 1553 1094 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4398 493 Size: 3905 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6607 SphI G_CATG'C Cuts at: 1132 3502 3965 1132 Size: 2370 463 3774 Fragments arranged by size: 3774 2370 463 SspI AAT'ATT Cuts at: 5 52 4531 5 Size: 47 4479 2081 Fragments arranged by size: 4479 2081 47 StuI AGG'CCT Cuts at: 1517 3482 1517 Size: 1965 4642 StyI C'CwwG_G Cuts at: 513 1065 2004 2559 3258 4046 513 Size: 552 939 555 699 788 3074 Fragments arranged by size: 3074 939 788 699 555 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3386 3871 4634 4651 4804 4989 5434 Size: 1255 1806 485 763 17 153 185 445 Cuts at: 5434 325 Size: 1498 Fragments arranged by size: 1806 1498 1255 763 485 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4853 6565 96 Size: 274 80 33 51 1443 2876 1712 138 Fragments arranged by size: 2876 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3382 3414 3424 3456 3846 3878 6567 6599 3382 Size: 32 10 32 390 32 2689 32 3390 Fragments arranged by size: 3390 2689 390 32 32 32 32 10 Tth111I GACn'n_nGTC Cuts at: 1316 2375 1316 Size: 1059 5548 XbaI T'CTAG_A Cuts at: 3943 3943 Size: 6607 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2131 2889 3043 2131 Size: 758 154 5695 Fragments arranged by size: 5695 758 154 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6607 XmaI C'CCGG_G Cuts at: 1870 3932 1870 Size: 2062 4545 XmnI GAAnn'nnTTC Cuts at: 3482 4736 3482 Size: 1254 5353 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4415 276 Size: 53 83 186 936 2881 2468 Fragments arranged by size: 2881 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BfrBI BsiWI BsmBI EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI