Restriction map for
pFN21AB7701
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BalI BamHI BanII BbrPI BbsI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BsrDI BsrGI Bst1107I
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI Eam1105I Eco47III Eco81I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI HpaI KpnI KspI MfeI
MluI MluNI MroI MscI MunI
NcoI NdeI NheI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcsI 832 GAATTT [GA]^AATT[CT]
AcyI 589 GGCGCC G[GA]^CG[CT]C
AflIII 1116 ACATGT A^C[GA][CT]GT
AlwNI 259 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 832 GAATTT [GA]^AATT[CT]
AviII 1109 TGCGCA TGC^GCA
BbeI 589 GGCGCC GGCGC^C
BbvCI 555 CCTCAGC CCTCAGC(-5/-2)
BcgI 281 CGACCCAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 839 CTAG C^TAG
BglI 568 GCCGGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 390 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 589 GGCGCC G[GA]^CG[CT]C
BsaWI 1132 ACCGGA [AT]^CCGG[AT]
BsmFI 962 GTCCC GGGAC(10/14)
BspLU11I 1116 ACATGT A^CATGT
BspMI 624 GCAGGT ACCTGC(4/8)
BssHII 363 GCGCGC G^CGCGC
BssSI 70 CACGAG CACGAG(-5/-1)
BstAPI 829 GCAGAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1069 TTCGAA TT^CGAA
BstYI 813 AGATCC [GA]^GATC[CT]
DraII 1141 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 663 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 1141 AGGCCCT [GA]G^G[ACGT]CC[CT]
FspI 1109 TGCGCA TGC^GCA
HaeII 589 GGCGCC [GA]GCGC^[CT]
Hin1I 589 GGCGCC G[GA]^CG[CT]C
HincII 1188 GTCAAC GT[CT]^[GA]AC
HindII 1188 GTCAAC GT[CT]^[GA]AC
HindIII 995 AAGCTT A^AGCTT
KasI 589 GGCGCC G^GCGCC
MaeI 839 CTAG C^TAG
MamI 390 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 813 AGATCC [GA]^GATC[CT]
NarI 589 GGCGCC GG^CGCC
NotI 506 GCGGCCGC GC^GGCCGC
NspV 1069 TTCGAA TT^CGAA
PstI 256 CTGCAG CTGCA^G
SapI 534 GAAGAGC GCTCTTC(1/4)
SfcI 256 CTGCAG C^T[GA][CT]AG
SfoI 589 GGCGCC GGC^GCC
SfuI 1069 TTCGAA TT^CGAA
SmaI 597 CCCGGG CCC^GGG
XhoII 813 AGATCC [GA]^GATC[CT]
XmaCI 597 CCCGGG C^CCGGG
XmaI 597 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Alw44I 289 GTGCAC G^TGCAC
1062 GTGCAC
ApaLI 289 GTGCAC G^TGCAC
1062 GTGCAC
AspHI 289 GTGCAC G[AT]GC[AT]^C
1062 GTGCAC
AvaI 6 CTCGGG C^[CT]CG[GA]G
597 CCCGGG
AvaII 671 GGTCC G^G[AT]CC
961 GGTCC
BanI 402 GGTGCC G^G[CT][GA]CC
589 GGCGCC
BsiHKAI 289 GTGCAC G[AT]GC[AT]^C
1062 GTGCAC
BsoBI 6 CTCGGG C^[CT]CG[GA]G
597 CCCGGG
BsrBI 474 GAGCGG CCGCTC(-3/-3)
537 GAGCGG
BstF5I 90 CATCC GGATG(2/0)
396 CATCC
EarI 534 GAAGAG CTCTTC(1/4)
546 GAAGAG
EcoNI 781 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
895 CCTCATAAAGG
FokI 90 CATCC GGATG(9/13)
396 CATCC
HgaI 321 GACGC GACGC(5/10)
512 GCGTC
Ksp632I 534 GAAGAG CTCTTC(1/4)
546 GAAGAG
MaeII 974 ACGT A^CGT
1066 ACGT
MseI 860 TTAA T^TAA
1213 TTAA
MspA1I 262 CCGCTG C[AC]G^C[GT]G
558 CAGCGG
NspI 1116 ACATGT [GA]CATG^[CT]
1218 ACATGC
PleI 349 GACTC GAGTC(4/5)
968 GAGTC
SfaNI 45 GATGC GCATC(5/9)
871 GATGC
TfiI 390 GATTC G^A[AT]TC
1124 GAATC
Tru9I 860 TTAA T^TAA
1213 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AluI AlwI BbvI
BcnI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiEI BsiYI BslI
BsmAI Bsp1286I BsrFI BsrI BssKI
BstEII BstNI BstPI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DsaI EaeI EagI EclXI
Eco52I EcoO65I EcoRII FauI Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MslI MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV Sau3AI Sau96I
ScrFI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I