(Circular) (Six-base) MAPSORT of: pFN21AB7701.seq Check: 7362 from: 1 to: 5947 pFN21AB7701 5947 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2620 2620 Size: 5947 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3757 278 Size: 53 83 186 936 2221 2468 Fragments arranged by size: 2468 2221 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3290 1937 Size: 1353 4594 Acc65I G'GTAC_C Cuts at: 3268 3268 Size: 5947 AclI AA'CG_TT Cuts at: 1563 4074 4447 1563 Size: 2511 373 3063 Fragments arranged by size: 3063 2511 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4010 5394 5662 1632 Size: 2378 1384 268 1917 Fragments arranged by size: 2378 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5750 AflIII A'CryG_T Cuts at: 1203 3121 4846 5823 1203 Size: 1918 1725 977 1327 Fragments arranged by size: 1918 1725 1327 977 AgeI A'CCGG_T Cuts at: 4778 4778 Size: 5947 AhdI GACnn_n'nnGTC Cuts at: 4676 4676 Size: 5947 AleI CACnn'nnGTG Cuts at: 1166 2123 2982 3122 1166 Size: 957 859 140 3991 Fragments arranged by size: 3991 957 859 140 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3140 1184 Size: 1956 3991 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2642 2674 2642 Size: 32 5915 AlwNI CAG_nnn'CTG Cuts at: 2269 3690 5262 2269 Size: 1421 1572 2954 Fragments arranged by size: 2954 1572 1421 ApaLI G'TGCA_C Cuts at: 2294 3067 4004 5160 2294 Size: 773 937 1156 3081 Fragments arranged by size: 3081 1156 937 773 ApoI r'AATT_y Cuts at: 1493 2837 3256 3482 3634 5743 1493 Size: 1344 419 226 152 2109 1697 Fragments arranged by size: 2109 1697 1344 419 226 152 AseI AT'TA_AT Cuts at: 160 1032 4501 160 Size: 872 3469 1606 Fragments arranged by size: 3469 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5947 AvaI C'yCGr_G Cuts at: 1870 1943 2011 2602 3272 1870 Size: 73 68 591 670 4545 Fragments arranged by size: 4545 670 591 73 68 BamHI G'GATC_C Cuts at: 3277 3644 3277 Size: 367 5580 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2407 2594 3268 4723 618 Size: 325 217 627 620 187 674 1455 1842 Fragments arranged by size: 1842 1455 674 627 620 325 217 187 BanII G_rGCy'C Cuts at: 1391 3266 1391 Size: 1875 4072 BbeI G_GCGC'C Cuts at: 2598 2598 Size: 5947 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4976 BbvCI CC'TCA_GC Cuts at: 2561 2561 Size: 5947 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2275 2309 4138 4172 1903 Size: 34 338 34 1829 34 3678 Fragments arranged by size: 3678 1829 338 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3839 5049 3839 Size: 1210 4737 BclI T'GATC_A Cuts at: 1548 1548 Size: 5947 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2579 4558 136 Size: 107 122 71 631 605 907 1979 1525 Fragments arranged by size: 1979 1525 907 631 605 122 107 71 BglII A'GATC_T Cuts at: 5942 5942 Size: 5947 BlpI GC'TnA_GC Cuts at: 3364 3364 Size: 5947 Bme1580I G_kGCm'C Cuts at: 1790 2205 2298 2412 3071 3161 4008 5164 1790 Size: 415 93 114 659 90 847 1156 2573 Fragments arranged by size: 2573 1156 847 659 415 114 93 90 BmgBI CAC'GTC Cuts at: 1318 2980 1318 Size: 1662 4285 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3678 4636 442 Size: 416 53 83 399 24 2261 958 1753 Fragments arranged by size: 2261 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5947 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2669 2811 3069 4607 1239 Size: 1430 142 258 1538 2579 Fragments arranged by size: 2579 1538 1430 258 142 Bpu10I CC'TnA_GC Cuts at: 2561 3228 2561 Size: 667 5280 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4072 4937 5235 4072 Size: 865 298 4784 Fragments arranged by size: 4784 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2084 2099 2108 4610 882 Size: 617 585 15 9 2502 2219 Fragments arranged by size: 2502 2219 617 585 15 9 BsaAI yAC'GTr Cuts at: 493 1204 3738 493 Size: 711 2534 2702 Fragments arranged by size: 2702 2534 711 BsaBI GATnn'nnATC Cuts at: 2399 5941 2399 Size: 3542 2405 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2595 3754 4136 4771 Size: 53 83 186 936 1062 1159 382 635 Cuts at: 4771 275 Size: 1451 Fragments arranged by size: 1451 1159 1062 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3137 4380 4778 5052 5199 5610 3137 Size: 1243 398 274 147 411 3474 Fragments arranged by size: 3474 1243 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2642 2672 1178 Size: 30 165 30 1239 30 4453 Fragments arranged by size: 4453 1239 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2147 2150 2153 2156 2348 1186 Size: 961 3 3 3 192 4785 Fragments arranged by size: 4785 961 192 3 3 3 BseYI C'CCAG_C Cuts at: 5150 5646 5150 Size: 496 5451 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2057 2342 2654 2896 2939 3006 1163 Size: 573 321 285 312 242 43 67 4104 Fragments arranged by size: 4104 573 321 312 285 242 67 43 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2058 2421 2472 2515 3397 4158 Size: 275 186 57 363 51 43 882 761 Cuts at: 4158 5186 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 882 761 363 275 186 57 51 43 BsiHKAI G_wGCw'C Cuts at: 2298 3071 4008 4093 5164 2298 Size: 773 937 85 1071 3081 Fragments arranged by size: 3081 1071 937 773 85 BsmI GAATG_Cn' Cuts at: 3458 3551 3458 Size: 93 5854 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2205 2298 2412 3071 3161 3266 4008 Size: 399 415 93 114 659 90 105 742 Cuts at: 4008 4093 5164 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 659 415 399 114 105 93 90 85 BspEI T'CCGG_A Cuts at: 5610 5610 Size: 5947 BspHI T'CATG_A Cuts at: 3836 5566 3836 Size: 1730 4217 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2620 3302 844 Size: 1776 682 3489 Fragments arranged by size: 3489 1776 682 BsrBI CCG'CTC Cuts at: 1394 1823 2481 2544 3834 1394 Size: 429 658 63 1290 3507 Fragments arranged by size: 3507 1290 658 429 63 BsrDI GCAATG_nn' Cuts at: 65 1248 4442 4616 65 Size: 1183 3194 174 1396 Fragments arranged by size: 3194 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2053 2207 2416 2420 2471 2573 4591 Size: 110 243 154 209 4 51 102 2018 Cuts at: 4591 4778 1700 Size: 187 2869 Fragments arranged by size: 2869 2018 243 209 187 154 110 102 51 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5947 BssHII G'CGCG_C Cuts at: 1925 2368 1925 Size: 443 5504 BssSI C'ACGA_G Cuts at: 2075 4007 5019 2075 Size: 1932 1012 3003 Fragments arranged by size: 3003 1932 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2840 794 Size: 2046 3901 BstBI TT'CG_AA Cuts at: 3075 4751 3075 Size: 1676 4271 BstEII G'GTnAC_C Cuts at: 1184 2272 2455 2665 1184 Size: 1088 183 210 4466 Fragments arranged by size: 4466 1088 210 183 BstXI CCAn_nnnn'nTGG Cuts at: 2101 2125 2830 2101 Size: 24 705 5218 Fragments arranged by size: 5218 705 24 BstYI r'GATC_y Cuts at: 1972 2818 3277 3644 4029 4046 5498 5584 5596 Size: 846 459 367 385 17 1452 86 12 Cuts at: 5596 5942 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 846 459 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2034 2179 2197 2460 2962 513 Size: 552 939 30 145 18 263 502 3498 Fragments arranged by size: 3498 939 552 502 263 145 30 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2014 487 Size: 683 56 788 4420 Fragments arranged by size: 4420 788 683 56 BtsI GCAGTG_nn' Cuts at: 3463 4257 4277 3463 Size: 794 20 5133 Fragments arranged by size: 5133 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5947 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5912 DraI TTT'AAA Cuts at: 3251 3251 Size: 5947 DraIII CAC_nnn'GTG Cuts at: 2673 2673 Size: 5947 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4954 809 Size: 507 3638 1802 Fragments arranged by size: 3638 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2194 2209 Size: 54 1425 50 275 18 225 139 15 Cuts at: 2209 2418 2469 2512 2937 3394 4283 5912 8 Size: 209 51 43 425 457 889 1629 43 Fragments arranged by size: 1629 1425 889 457 425 275 225 209 139 54 51 50 43 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2055 2418 2469 2512 3394 1537 Size: 275 243 363 51 43 882 4090 Fragments arranged by size: 4090 882 363 275 243 51 43 EarI CTCTTCn'nnn_ Cuts at: 1353 2534 2546 3877 1353 Size: 1181 12 1331 3423 Fragments arranged by size: 3423 1331 1181 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4534 4908 5054 387 Size: 1064 138 2945 374 146 1280 Fragments arranged by size: 2945 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2669 2811 3069 4010 4607 5394 5662 Size: 393 1037 142 258 941 597 787 268 Cuts at: 5662 1239 Size: 1524 Fragments arranged by size: 1524 1037 941 787 597 393 268 258 142 EcoNI CCTnn'n_nnAGG Cuts at: 2790 2904 2790 Size: 114 5833 EcoO109I rG'GnC_Cy Cuts at: 3147 3147 Size: 5947 EcoRI G'AATT_C Cuts at: 3256 3634 3256 Size: 378 5569 FspI TGC'GCA Cuts at: 3116 4453 3116 Size: 1337 4610 HaeII r_GCGC'y Cuts at: 1678 2598 5094 1678 Size: 920 2496 2531 Fragments arranged by size: 2531 2496 920 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2935 2964 2967 2996 4588 Size: 32 135 32 1363 29 3 29 1592 Cuts at: 4588 4620 4662 4694 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1592 1363 135 42 32 32 32 32 29 29 3 HincII GTy'rAC Cuts at: 669 1657 1938 3195 3291 3537 669 Size: 988 281 1257 96 246 3079 Fragments arranged by size: 3079 1257 988 281 246 96 HindIII A'AGCT_T Cuts at: 748 3000 748 Size: 2252 3695 HpaI GTT'AAC Cuts at: 3537 3537 Size: 5947 KasI G'GCGC_C Cuts at: 2594 2594 Size: 5947 KpnI G_GTAC'C Cuts at: 3272 3272 Size: 5947 MfeI C'AATT_G Cuts at: 3546 3546 Size: 5947 MluI A'CGCG_T Cuts at: 5823 5823 Size: 5947 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5061 5245 1305 Size: 3756 184 2007 Fragments arranged by size: 3756 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4125 Fragments arranged by size: 4125 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2099 2123 2405 2918 2982 3122 3905 Size: 648 933 24 282 513 64 140 783 Cuts at: 3905 4264 4423 518 Size: 359 159 2042 Fragments arranged by size: 2042 933 783 648 513 359 282 159 140 64 24 MspA1I CmG'CkG Cuts at: 1606 2269 2565 3363 4040 5188 5433 1606 Size: 663 296 798 677 1148 245 2120 Fragments arranged by size: 2120 1148 798 677 663 296 245 NaeI GCC'GGC Cuts at: 2055 2209 2418 2422 2473 2575 2055 Size: 154 209 4 51 102 5427 Fragments arranged by size: 5427 209 154 102 51 4 NarI GG'CG_CC Cuts at: 2595 2595 Size: 5947 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4456 Fragments arranged by size: 4456 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5947 NgoMIV G'CCGG_C Cuts at: 2053 2207 2416 2420 2471 2573 2053 Size: 154 209 4 51 102 5427 Fragments arranged by size: 5427 209 154 102 51 4 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5947 NotI GC'GGCC_GC Cuts at: 2512 3394 2512 Size: 882 5065 NspI r_CATG'y Cuts at: 1132 1229 3125 3227 3305 4850 1132 Size: 97 1896 102 78 1545 2229 Fragments arranged by size: 2229 1896 1545 102 97 78 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5947 PciI A'CATG_T Cuts at: 3121 4846 3121 Size: 1725 4222 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5947 PfoI T'CCnGG_A Cuts at: 2788 2788 Size: 5947 PmeI GTTT'AAAC Cuts at: 3251 3251 Size: 5947 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2642 2674 4016 4048 5529 5561 2642 Size: 32 1342 32 1481 32 3028 Fragments arranged by size: 3028 1481 1342 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5947 PsiI TTA'TAA Cuts at: 3517 3517 Size: 5947 PstI C_TGCA'G Cuts at: 830 2265 3299 830 Size: 1435 1034 3478 Fragments arranged by size: 3478 1435 1034 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5947 SalI G'TCGA_C Cuts at: 1936 3289 1936 Size: 1353 4594 SapI GCTCTTCn'nnn_ Cuts at: 2534 2534 Size: 5947 SbfI CC_TGCA'GG Cuts at: 3299 3299 Size: 5947 ScaI AGT'ACT Cuts at: 4195 5907 4195 Size: 1712 4235 SexAI A'CCwGG_T Cuts at: 3243 3243 Size: 5947 SfcI C'TryA_G Cuts at: 826 1045 2261 3295 4430 5111 5302 826 Size: 219 1216 1034 1135 681 191 1471 Fragments arranged by size: 1471 1216 1135 1034 681 219 191 SfoI GGC'GCC Cuts at: 2596 2596 Size: 5947 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5947 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5947 SmaI CCC'GGG Cuts at: 1872 2604 3274 1872 Size: 732 670 4545 Fragments arranged by size: 4545 732 670 SmlI C'TyrA_G Cuts at: 820 1017 1943 4051 4952 5214 820 Size: 197 926 2108 901 262 1553 Fragments arranged by size: 2108 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3738 493 Size: 3245 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5947 SphI G_CATG'C Cuts at: 1132 3305 1132 Size: 2173 3774 SspI AAT'ATT Cuts at: 5 52 3871 5 Size: 47 3819 2081 Fragments arranged by size: 3819 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5947 StyI C'CwwG_G Cuts at: 513 1065 2004 3386 513 Size: 552 939 1382 3074 Fragments arranged by size: 3074 1382 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2653 2975 3974 3991 4144 4329 4774 Size: 1255 1073 322 999 17 153 185 445 Cuts at: 4774 325 Size: 1498 Fragments arranged by size: 1498 1255 1073 999 445 322 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4193 5905 96 Size: 274 80 33 51 1443 2216 1712 138 Fragments arranged by size: 2216 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2950 2982 5907 5939 2950 Size: 32 2925 32 2958 Fragments arranged by size: 2958 2925 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5947 XbaI T'CTAG_A Cuts at: 3283 3283 Size: 5947 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5947 XmaI C'CCGG_G Cuts at: 1870 2602 3272 1870 Size: 732 670 4545 Fragments arranged by size: 4545 732 670 XmnI GAAnn'nnTTC Cuts at: 4076 4076 Size: 5947 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3755 276 Size: 53 83 186 936 2221 2468 Fragments arranged by size: 2468 2221 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SrfI SwaI XcmI