Restriction map for
pFN21AE0340
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BfrI BglII BlnI
BlpI Bpu1102I BsaHI BsaI BsaWI
BseAI BsgI BsiEI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HgaI Hin1I HincII
HindII HpaI KasI KpnI Ksp632I
MluI MroI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 535 GTAGAC GT^[AC][GT]AC
AflIII 1030 ACACGT A^C[GA][CT]GT
AocI 1066 CCTCAGG CC^T[ACGT]AGG
AspHI 295 GTGCTC G[AT]GC[AT]^C
AvaI 1276 CTCGGG C^[CT]CG[GA]G
AvaII 493 GGTCC G^G[AT]CC
BbsI 1005 GTCTTC GAAGAC(2/6)
BclI 734 TGATCA T^GATCA
BpuAI 1005 GTCTTC GAAGAC(2/6)
BsaBI 1335 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 169 CTCCTC GAGGAG(10/8)
BsiHKAI 295 GTGCTC G[AT]GC[AT]^C
BsiWI 1033 CGTACG C^GTACG
BsmBI 592 GAGACG CGTCTC(1/5)
BsoBI 1276 CTCGGG C^[CT]CG[GA]G
BspHI 1202 TCATGA T^CATGA
BspMI 1052 GCAGGT ACCTGC(4/8)
BssSI 875 CTCGTG CACGAG(-5/-1)
Bsu36I 1066 CCTCAGG CC^T[ACGT]AGG
Cac8I 140 GCCAGC GC[ACGT]^[ACGT]GC
DraII 622 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 940 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 51 CCGCGG C^C[GA][CT]GG
Eco81I 1066 CCTCAGG CC^T[ACGT]AGG
EcoNI 663 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 622 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 4 CCTTGG C^C[AT][AT]GG
FauI 34 CCCGC CCCGC(4/6)
FbaI 734 TGATCA T^GATCA
HaeII 722 AGCGCC [GA]GCGC^[CT]
KspI 51 CCGCGG CCGC^GG
MamI 1335 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1346 CAATTG C^AATTG
MslI 1031 CACGTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1346 CAATTG C^AATTG
NlaIV 30 GGTTCC GG[ACGT]^[ACGT]CC
PvuII 712 CAGCTG CAG^CTG
RcaI 1202 TCATGA T^CATGA
SacII 51 CCGCGG CCGC^GG
SmlI 260 CTCAAG C^T[CT][GA]AG
StyI 4 CCTTGG C^C[AT][AT]GG
XbaI 1092 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 52 CGCG CG^CG
497 CGCG
AcsI 559 GAATTT [GA]^AATT[CT]
859 GAATTT
AlwNI 658 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
709 CAGCAGCTG
ApoI 559 GAATTT [GA]^AATT[CT]
859 GAATTT
BalI 1039 TGGCCA TGG^CCA
1266 TGGCCA
BanII 872 GGGCTC G[GA]GC[CT]^C
1351 GAGCCC
BcnI 870 CCGGG CC^[GC]GG
1354 CCCGG
BfaI 553 CTAG C^TAG
1093 CTAG
BglI 413 GCCTCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1228 GCCTTCAAGGC
BsaAI 1031 CACGTA [CT]AC^GT[GA]
1035 TACGTG
BstUI 52 CGCG CG^CG
497 CGCG
BstYI 468 AGATCC [GA]^GATC[CT]
1097 AGATCC
CfoI 723 GCGC GCG^C
881 GCGC
EaeI 1039 TGGCCA [CT]^GGCC[GA]
1266 TGGCCA
HhaI 723 GCGC GCG^C
881 GCGC
HinP1I 723 GCGC G^CGC
881 GCGC
HindIII 1137 AAGCTT A^AGCTT
1183 AAGCTT
MaeI 553 CTAG C^TAG
1093 CTAG
MflI 468 AGATCC [GA]^GATC[CT]
1097 AGATCC
MluNI 1039 TGGCCA TGG^CCA
1266 TGGCCA
MscI 1039 TGGCCA TGG^CCA
1266 TGGCCA
MseI 1187 TTAA T^TAA
1214 TTAA
MvnI 52 CGCG CG^CG
497 CGCG
NciI 870 CCGGG CC^[GC]GG
1354 CCCGG
NspI 630 ACATGC [GA]CATG^[CT]
1304 ACATGC
PflMI 282 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1042 CCAGTCAATGG
SfaNI 1161 GATGC GCATC(5/9)
1384 GATGC
TaqI 477 TCGA T^CGA
1242 TCGA
TfiI 120 GATTC G^A[AT]TC
377 GATTC
Tru9I 1187 TTAA T^TAA
1214 TTAA
Tsp45I 1022 GTGAC ^GT[GC]AC
1438 GTGAC
TspRI 287 CAGTG CA[GC]TG[ACGT][ACGT]^
507 CACTG
TthHB8I 477 TCGA T^CGA
1242 TCGA
Van91I 282 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1042 CCAGTCAATGG
XhoII 468 AGATCC [GA]^GATC[CT]
1097 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AlwI Asp700I
BbvI BmyI BpmI BsaJI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NdeII NlaIII PleI PstI RsaI
Sau3AI Sau96I ScrFI SfcI TseI
Tsp509I XmnI