(Circular) (Six-base) MAPSORT of: pFN21AE0340.seq Check: 1937 from: 1 to: 6226 pFN21AE0340 6226 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4036 278 Size: 53 83 186 936 2500 2468 Fragments arranged by size: 2500 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2541 3569 1937 Size: 604 1028 4594 Fragments arranged by size: 4594 1028 604 Acc65I G'GTAC_C Cuts at: 3547 3547 Size: 6226 AclI AA'CG_TT Cuts at: 1563 4353 4726 1563 Size: 2790 373 3063 Fragments arranged by size: 3063 2790 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2087 2396 4289 5673 5941 1632 Size: 455 309 1893 1384 268 1917 Fragments arranged by size: 1917 1893 1384 455 309 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6029 AflIII A'CryG_T Cuts at: 1203 3035 5125 6102 1203 Size: 1832 2090 977 1327 Fragments arranged by size: 2090 1832 1327 977 AgeI A'CCGG_T Cuts at: 5057 5057 Size: 6226 AhdI GACnn_n'nnGTC Cuts at: 4955 4955 Size: 6226 AleI CACnn'nnGTG Cuts at: 1166 3040 1166 Size: 1874 4352 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6226 AlwNI CAG_nnn'CTG Cuts at: 2668 2719 3969 5541 2668 Size: 51 1250 1572 3353 Fragments arranged by size: 3353 1572 1250 51 ApaLI G'TGCA_C Cuts at: 4283 5439 4283 Size: 1156 5070 ApoI r'AATT_y Cuts at: 1493 2564 2864 3535 3761 3913 6022 1493 Size: 1071 300 671 226 152 2109 1697 Fragments arranged by size: 2109 1697 1071 671 300 226 152 AseI AT'TA_AT Cuts at: 160 1032 4780 160 Size: 872 3748 1606 Fragments arranged by size: 3748 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6226 AvaI C'yCGr_G Cuts at: 1870 1943 3281 3551 1870 Size: 73 1338 270 4545 Fragments arranged by size: 4545 1338 270 73 BamHI G'GATC_C Cuts at: 3556 3923 3556 Size: 367 5859 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3547 5002 618 Size: 325 217 627 1760 1455 1842 Fragments arranged by size: 1842 1760 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2881 3360 3545 1391 Size: 1490 479 185 4072 Fragments arranged by size: 4072 1490 479 185 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3003 928 Size: 971 1104 4151 Fragments arranged by size: 4151 1104 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4417 4451 1903 Size: 34 50 34 2396 34 3678 Fragments arranged by size: 3678 2396 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4118 5328 4118 Size: 1210 5016 BclI T'GATC_A Cuts at: 1548 2739 1548 Size: 1191 5035 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2424 3239 4837 Size: 107 122 71 631 605 752 815 1598 Cuts at: 4837 136 Size: 1525 Fragments arranged by size: 1598 1525 815 752 631 605 122 107 71 BglII A'GATC_T Cuts at: 6221 6221 Size: 6226 BlpI GC'TnA_GC Cuts at: 3643 3643 Size: 6226 Bme1580I G_kGCm'C Cuts at: 1790 4287 5443 1790 Size: 2497 1156 2573 Fragments arranged by size: 2573 2497 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6226 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2472 3957 4915 Size: 416 53 83 399 24 1055 1485 958 Cuts at: 4915 442 Size: 1753 Fragments arranged by size: 1753 1485 1055 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6226 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2181 2556 3288 4886 1239 Size: 942 375 732 1598 2579 Fragments arranged by size: 2579 1598 942 732 375 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2250 4351 5216 5514 2250 Size: 2101 865 298 2962 Fragments arranged by size: 2962 2101 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4889 882 Size: 617 3390 2219 Fragments arranged by size: 3390 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3038 3042 4017 493 Size: 711 1834 4 975 2702 Fragments arranged by size: 2702 1834 975 711 4 BsaBI GATnn'nnATC Cuts at: 3344 6220 3344 Size: 2876 3350 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4033 4415 5050 275 Size: 53 83 186 936 2500 382 635 1451 Fragments arranged by size: 2500 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4659 5057 5331 5478 5889 4659 Size: 398 274 147 411 4996 Fragments arranged by size: 4996 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2715 2745 1178 Size: 30 165 30 1312 30 4659 Fragments arranged by size: 4659 1312 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2165 1186 Size: 979 5247 BseYI C'CCAG_C Cuts at: 2150 2777 5429 5925 2150 Size: 627 2652 496 2451 Fragments arranged by size: 2652 2451 627 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5653 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3676 4437 5465 1540 Size: 275 186 1675 761 1028 2301 Fragments arranged by size: 2301 1675 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2304 4287 4372 5443 2304 Size: 1983 85 1071 3087 Fragments arranged by size: 3087 1983 1071 85 BsiWI C'GTAC_G Cuts at: 3038 3038 Size: 6226 BsmI GAATG_Cn' Cuts at: 2400 2772 2876 3737 3830 2400 Size: 372 104 861 93 4796 Fragments arranged by size: 4796 861 372 104 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2591 2591 Size: 6226 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2304 2881 3360 3545 4287 4372 5443 Size: 399 514 577 479 185 742 85 1071 Cuts at: 5443 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 577 514 479 399 185 85 BspEI T'CCGG_A Cuts at: 5889 5889 Size: 6226 BspHI T'CATG_A Cuts at: 3207 4115 5845 3207 Size: 908 1730 3588 Fragments arranged by size: 3588 1730 908 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3048 3581 844 Size: 2204 533 3489 Fragments arranged by size: 3489 2204 533 BsrBI CCG'CTC Cuts at: 1394 1823 4113 1394 Size: 429 2290 3507 Fragments arranged by size: 3507 2290 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4721 4895 65 Size: 1183 3473 174 1396 Fragments arranged by size: 3473 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4870 5057 1700 Size: 110 3060 187 2869 Fragments arranged by size: 3060 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6226 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6226 BssSI C'ACGA_G Cuts at: 2880 4286 5298 2880 Size: 1406 1012 3808 Fragments arranged by size: 3808 1406 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6226 BstBI TT'CG_AA Cuts at: 5030 5030 Size: 6226 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6226 BstYI r'GATC_y Cuts at: 1972 2473 3102 3556 3923 4308 4325 5777 5863 Size: 501 629 454 367 385 17 1452 86 Cuts at: 5863 5875 6221 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 629 501 454 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3072 3072 Size: 6226 BtgI C'CryG_G Cuts at: 513 1065 2056 513 Size: 552 991 4683 Fragments arranged by size: 4683 991 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5487 Fragments arranged by size: 5487 683 56 BtsI GCAGTG_nn' Cuts at: 3742 4536 4556 3742 Size: 794 20 5412 Fragments arranged by size: 5412 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6226 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6191 DraI TTT'AAA Cuts at: 3530 3530 Size: 6226 DraIII CAC_nnn'GTG Cuts at: 2950 2950 Size: 6226 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5233 809 Size: 507 3917 1802 Fragments arranged by size: 3917 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3044 3271 3673 Size: 54 1425 50 275 18 1214 227 402 Cuts at: 3673 4562 6191 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1214 889 402 275 227 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3673 1537 Size: 275 1861 4090 Fragments arranged by size: 4090 1861 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4156 1353 Size: 2803 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4813 5187 5333 387 Size: 1064 138 3224 374 146 1280 Fragments arranged by size: 3224 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2181 2396 2556 3288 4289 4886 Size: 393 455 94 215 160 732 1001 597 Cuts at: 4886 5673 5941 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1001 787 732 597 455 393 268 215 160 94 EcoNI CCTnn'n_nnAGG Cuts at: 2672 2672 Size: 6226 EcoO109I rG'GnC_Cy Cuts at: 2628 2628 Size: 6226 EcoRI G'AATT_C Cuts at: 3535 3913 3535 Size: 378 5848 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2471 2864 2471 Size: 393 5833 FspI TGC'GCA Cuts at: 4732 4732 Size: 6226 HaeII r_GCGC'y Cuts at: 1678 2731 5373 1678 Size: 1053 2642 2531 Fragments arranged by size: 2642 2531 1053 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2731 2763 4867 4899 4941 Size: 32 135 32 1159 32 2104 32 42 Cuts at: 4941 4973 1373 Size: 32 2626 Fragments arranged by size: 2626 2104 1159 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3570 3816 669 Size: 988 281 1632 246 3079 Fragments arranged by size: 3079 1632 988 281 246 HindIII A'AGCT_T Cuts at: 748 3142 3188 748 Size: 2394 46 3786 Fragments arranged by size: 3786 2394 46 HpaI GTT'AAC Cuts at: 3816 3816 Size: 6226 KpnI G_GTAC'C Cuts at: 3551 3551 Size: 6226 MfeI C'AATT_G Cuts at: 3351 3825 3351 Size: 474 5752 MluI A'CGCG_T Cuts at: 6102 6102 Size: 6226 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2331 5340 5524 1305 Size: 1026 3009 184 2007 Fragments arranged by size: 3009 2007 1026 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3046 3273 10 Size: 54 1425 343 1214 227 2963 Fragments arranged by size: 2963 1425 1214 343 227 54 MslI CAynn'nnrTG Cuts at: 518 1166 3040 4184 4543 4702 518 Size: 648 1874 1144 359 159 2042 Fragments arranged by size: 2042 1874 1144 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2042 2058 2719 3642 4319 5467 5712 1606 Size: 436 16 661 923 677 1148 245 2120 Fragments arranged by size: 2120 1148 923 677 661 436 245 16 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5674 NdeI CA'TA_TG Cuts at: 387 387 Size: 6226 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6226 NotI GC'GGCC_GC Cuts at: 3673 3673 Size: 6226 NspI r_CATG'y Cuts at: 1132 1229 2639 3313 3584 5129 1132 Size: 97 1410 674 271 1545 2229 Fragments arranged by size: 2229 1545 1410 674 271 97 PasI CC'CwG_GG Cuts at: 1348 3134 1348 Size: 1786 4440 PciI A'CATG_T Cuts at: 5125 5125 Size: 6226 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2293 3053 1108 Size: 902 283 760 4281 Fragments arranged by size: 4281 902 760 283 PfoI T'CCnGG_A Cuts at: 2197 2197 Size: 6226 PmeI GTTT'AAAC Cuts at: 3530 3530 Size: 6226 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4295 4327 5808 5840 4295 Size: 32 1481 32 4681 Fragments arranged by size: 4681 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6226 PsiI TTA'TAA Cuts at: 3796 3796 Size: 6226 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2065 2097 2065 Size: 32 6194 PstI C_TGCA'G Cuts at: 830 2755 3333 3475 3578 830 Size: 1925 578 142 103 3478 Fragments arranged by size: 3478 1925 578 142 103 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6226 PvuII CAG'CTG Cuts at: 2719 2719 Size: 6226 SacII CC_GC'GG Cuts at: 2059 2059 Size: 6226 SalI G'TCGA_C Cuts at: 1936 3568 1936 Size: 1632 4594 SbfI CC_TGCA'GG Cuts at: 3578 3578 Size: 6226 ScaI AGT'ACT Cuts at: 4474 6186 4474 Size: 1712 4514 SfcI C'TryA_G Cuts at: 826 1045 2043 2751 3157 3329 3471 3574 4709 Size: 219 998 708 406 172 142 103 1135 Cuts at: 4709 5390 5581 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 998 708 681 406 219 191 172 142 103 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6226 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6226 SmaI CCC'GGG Cuts at: 1872 3553 1872 Size: 1681 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2265 4330 5231 5493 820 Size: 197 926 322 2065 901 262 1553 Fragments arranged by size: 2065 1553 926 901 322 262 197 SnaBI TAC'GTA Cuts at: 493 4017 493 Size: 3524 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6226 SphI G_CATG'C Cuts at: 1132 3584 1132 Size: 2452 3774 SspI AAT'ATT Cuts at: 5 52 4150 5 Size: 47 4098 2081 Fragments arranged by size: 4098 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6226 StyI C'CwwG_G Cuts at: 513 1065 2009 3665 513 Size: 552 944 1656 3074 Fragments arranged by size: 3074 1656 944 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2181 2919 4253 4270 4423 4608 5053 Size: 1255 601 738 1334 17 153 185 445 Cuts at: 5053 325 Size: 1498 Fragments arranged by size: 1498 1334 1255 738 601 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4472 6184 96 Size: 274 80 33 51 1443 2495 1712 138 Fragments arranged by size: 2495 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3447 3479 6186 6218 3447 Size: 32 2707 32 3455 Fragments arranged by size: 3455 2707 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6226 XbaI T'CTAG_A Cuts at: 3097 3562 3097 Size: 465 5761 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6226 XmaI C'CCGG_G Cuts at: 1870 3551 1870 Size: 1681 4545 XmnI GAAnn'nnTTC Cuts at: 2565 2844 3093 4355 2565 Size: 279 249 1262 4436 Fragments arranged by size: 4436 1262 279 249 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4034 276 Size: 53 83 186 936 2500 2468 Fragments arranged by size: 2500 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BstXI BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI