Restriction map for
pFN21AE0340
(Linear) (Six-base) MAPSORT of: KIEE0340.seq Check: 6928 from: 1 to: 1521
KIEE0340.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 0 536 1521
Size: 536 985
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 391 1521
Size: 82 309 1130
Fragments arranged by size:
1130 309 82
AflIII A'CryG_T
Cuts at: 0 1030 1521
Size: 1030 491
AleI CACnn'nnGTG
Cuts at: 0 1035 1521
Size: 1035 486
AlwNI CAG_nnn'CTG
Cuts at: 0 663 714 1521
Size: 663 51 807
Fragments arranged by size:
807 663 51
ApoI r'AATT_y
Cuts at: 0 559 859 1521
Size: 559 300 662
Fragments arranged by size:
662 559 300
AvaI C'yCGr_G
Cuts at: 0 1276 1521
Size: 1276 245
BanII G_rGCy'C
Cuts at: 0 876 1355 1521
Size: 876 479 166
Fragments arranged by size:
876 479 166
BbsI GAAGACnn'nnnn_
Cuts at: 0 998 1521
Size: 998 523
BclI T'GATC_A
Cuts at: 0 734 1521
Size: 734 787
BglI GCCn_nnn'nGGC
Cuts at: 0 419 1234 1521
Size: 419 815 287
Fragments arranged by size:
815 419 287
BmrI ACTGGGnnnn_n'
Cuts at: 0 467 1521
Size: 467 1054
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 551 1283 1521
Size: 176 375 732 238
Fragments arranged by size:
732 375 238 176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 1521
Size: 245 1276
BsaAI yAC'GTr
Cuts at: 0 1033 1037 1521
Size: 1033 4 484
Fragments arranged by size:
1033 484 4
BsaBI GATnn'nnATC
Cuts at: 0 1339 1521
Size: 1339 182
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 710 740 1521
Size: 710 30 781
Fragments arranged by size:
781 710 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 160 1521
Size: 160 1361
BseYI C'CCAG_C
Cuts at: 0 145 772 1521
Size: 145 627 749
Fragments arranged by size:
749 627 145
BsiHKAI G_wGCw'C
Cuts at: 0 299 1521
Size: 299 1222
BsiWI C'GTAC_G
Cuts at: 0 1033 1521
Size: 1033 488
BsmI GAATG_Cn'
Cuts at: 0 395 767 871 1521
Size: 395 372 104 650
Fragments arranged by size:
650 395 372 104
BsmBI CGTCTCn'nnnn_
Cuts at: 0 586 1521
Size: 586 935
Bsp1286I G_dGCh'C
Cuts at: 0 299 876 1355 1521
Size: 299 577 479 166
Fragments arranged by size:
577 479 299 166
BspHI T'CATG_A
Cuts at: 0 1202 1521
Size: 1202 319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1043 1521
Size: 1043 478
BssSI C'ACGA_G
Cuts at: 0 875 1521
Size: 875 646
BstYI r'GATC_y
Cuts at: 0 468 1097 1521
Size: 468 629 424
Fragments arranged by size:
629 468 424
Bsu36I CC'TnA_GG
Cuts at: 0 1067 1521
Size: 1067 454
BtgI C'CryG_G
Cuts at: 0 51 1521
Size: 51 1470
DraIII CAC_nnn'GTG
Cuts at: 0 945 1521
Size: 945 576
EaeI y'GGCC_r
Cuts at: 0 1039 1266 1521
Size: 1039 227 255
Fragments arranged by size:
1039 255 227
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 176 391 551 1283 1521
Size: 82 94 215 160 732 238
Fragments arranged by size:
732 238 215 160 94 82
EcoNI CCTnn'n_nnAGG
Cuts at: 0 667 1521
Size: 667 854
EcoO109I rG'GnC_Cy
Cuts at: 0 623 1521
Size: 623 898
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 466 859 1521
Size: 466 393 662
Fragments arranged by size:
662 466 393
HaeII r_GCGC'y
Cuts at: 0 726 1521
Size: 726 795
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 726 758 1521
Size: 726 32 763
Fragments arranged by size:
763 726 32
HindIII A'AGCT_T
Cuts at: 0 1137 1183 1521
Size: 1137 46 338
Fragments arranged by size:
1137 338 46
MfeI C'AATT_G
Cuts at: 0 1346 1521
Size: 1346 175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 1521
Size: 326 1195
MscI TGG'CCA
Cuts at: 0 1041 1268 1521
Size: 1041 227 253
Fragments arranged by size:
1041 253 227
MslI CAynn'nnrTG
Cuts at: 0 1035 1521
Size: 1035 486
MspA1I CmG'CkG
Cuts at: 0 37 53 714 1521
Size: 37 16 661 807
Fragments arranged by size:
807 661 37 16
NspI r_CATG'y
Cuts at: 0 634 1308 1521
Size: 634 674 213
Fragments arranged by size:
674 634 213
PasI CC'CwG_GG
Cuts at: 0 1129 1521
Size: 1129 392
PflMI CCAn_nnn'nTGG
Cuts at: 0 288 1048 1521
Size: 288 760 473
Fragments arranged by size:
760 473 288
PfoI T'CCnGG_A
Cuts at: 0 192 1521
Size: 192 1329
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 60 92 1521
Size: 60 32 1429
Fragments arranged by size:
1429 60 32
PstI C_TGCA'G
Cuts at: 0 750 1328 1470 1521
Size: 750 578 142 51
Fragments arranged by size:
750 578 142 51
PvuII CAG'CTG
Cuts at: 0 714 1521
Size: 714 807
SacII CC_GC'GG
Cuts at: 0 54 1521
Size: 54 1467
SfcI C'TryA_G
Cuts at: 0 38 746 1152 1324 1466 1521
Size: 38 708 406 172 142 55
Fragments arranged by size:
708 406 172 142 55 38
SmlI C'TyrA_G
Cuts at: 0 260 1521
Size: 260 1261
StyI C'CwwG_G
Cuts at: 0 4 1521
Size: 4 1517
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 176 914 1521
Size: 176 738 607
Fragments arranged by size:
738 607 176
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1442 1474 1521
Size: 1442 32 47
Fragments arranged by size:
1442 47 32
XbaI T'CTAG_A
Cuts at: 0 1092 1521
Size: 1092 429
XmnI GAAnn'nnTTC
Cuts at: 0 560 839 1088 1521
Size: 560 279 249 433
Fragments arranged by size:
560 433 279 249
Enzymes that do cut:
AccI AcuI AflIII AleI AlwNI ApoI AvaI BanII
BbsI BclI BglI BmrI BpmI BpuEI BsaAI BsaBI
BsaXI BseRI BseYI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspHI BspMI BssSI BstYI Bsu36I BtgI DraIII EaeI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII MfeI
MmeI MscI MslI MspA1I NspI PasI PflMI PfoI
PsrI PstI PvuII SacII SfcI SmlI StyI TaqII
TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BfrBI
BglII BlpI Bme1580I BmgBI BmtI BplI Bpu10I BsaI
BsaHI BsaWI BsgI BsiEI BspEI BsrBI BsrDI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I BtgZI
BtsI ClaI CspCI DraI DrdI EagI EarI EciI
EcoICRI EcoRI EcoRV FseI FspI FspAI HincII HpaI
KasI KpnI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI Tth111I XcmI XhoI XmaI
ZraI