Restriction map for
pFN21AE3313
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# NO CUT ENZYMES ( 134 enzymes) #
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AatII Acc65I AccII AccIII AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BcnI BlnI BlpI
BmyI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1286I BspDI
BspEI BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstEII BstPI BstUI
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT22I FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HpaI HpaII KasI KpnI KspI
MamI MluI MroI MspA1I MspI
MvnI NaeI NarI NciI NdeI
NgoMIV NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI SexAI SfcI SfiI
SfoI SgrAI SmaI SnaBI SphI
Sse8387I StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 820 GTATAC GT^[AC][GT]AC
AclI 685 AACGTT AA^CGTT
AflII 567 CTTAAG C^TTAAG
BalI 1859 TGGCCA TGG^CCA
BamHI 1824 GGATCC G^GATCC
BbsI 243 GAAGAC GAAGAC(2/6)
BbvI 2307 GCTGC GCAGC(8/12)
BclI 1392 TGATCA T^GATCA
BfrI 567 CTTAAG C^TTAAG
BglI 1672 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1884 AGATCT A^GATCT
BpmI 75 CTGGAG CTGGAG(16/14)
BpuAI 243 GAAGAC GAAGAC(2/6)
BsaI 278 GAGACC GGTCTC(1/5)
BsmI 2014 GAATGC GAATGC(1/-1)
Bsp1407I 136 TGTACA T^GTACA
BspHI 2043 TCATGA T^CATGA
BspLU11I 1397 ACATGT A^CATGT
BsrGI 136 TGTACA T^GTACA
Bst1107I 820 GTATAC GTA^TAC
BstBI 713 TTCGAA TT^CGAA
BstXI 67 CCATTCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 820 GTATAC GTA^TAC
DraI 2110 TTTAAA TTT^AAA
DraIII 1331 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2035 CCATGG C^C[GA][CT]GG
EaeI 1859 TGGCCA [CT]^GGCC[GA]
EcoT14I 2035 CCATGG C^C[AT][AT]GG
FauI 399 CCCGC CCCGC(4/6)
FbaI 1392 TGATCA T^GATCA
Fnu4HI 2307 GCTGC GC^[ACGT]GC
HindIII 229 AAGCTT A^AGCTT
ItaI 2307 GCTGC GC^[ACGT]GC
MfeI 2142 CAATTG C^AATTG
MluNI 1859 TGGCCA TGG^CCA
MscI 1859 TGGCCA TGG^CCA
MslI 1105 CATTTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 2142 CAATTG C^AATTG
NcoI 2035 CCATGG C^CATGG
NheI 910 GCTAGC G^CTAGC
NspI 1397 ACATGT [GA]CATG^[CT]
NspV 713 TTCGAA TT^CGAA
Psp1406I 685 AACGTT AA^CGTT
RcaI 2043 TCATGA T^CATGA
SfuI 713 TTCGAA TT^CGAA
SpeI 414 ACTAGT A^CTAGT
SspBI 136 TGTACA T^GTACA
SspI 1871 AATATT AAT^ATT
StyI 2035 CCATGG C^C[AT][AT]GG
TseI 2307 GCTGC G^C[AT]GC
XcmI 2263 CCAGAAATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AciI 400 CCGC CCGC(-3/-1)
2049 CCGC
AflIII 1397 ACATGT A^C[GA][CT]GT
1529 ACACGT
Asp700I 883 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1870 GAATATTTTC
BseRI 1283 CTCCTC GAGGAG(10/8)
145 GAGGAG
BsmAI 278 GAGAC GTCTC(1/5)
1467 GAGAC
BsmFI 939 GGGAC GGGAC(10/14)
2253 GGGAC
BsrDI 945 CATTGC GCAATG(2/0)
1039 GCAATG
EarI 299 CTCTTC CTCTTC(1/4)
2222 CTCTTC
EcoRV 725 GATATC GAT^ATC
1305 GATATC
HaeIII 390 GGCC GG^CC
1860 GGCC
Ksp632I 299 CTCTTC CTCTTC(1/4)
2222 CTCTTC
ScaI 1633 AGTACT AGT^ACT
1648 AGTACT
SfaNI 2485 GATGC GCATC(5/9)
1618 GCATC
SmlI 567 CTTAAG C^T[CT][GA]AG
1145 CTCAAG
XmnI 883 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1870 GAATATTTTC
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AseI AsnI AvaII BfaI
BsaJI BsiYI BslI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
FokI HincII HindII HinfI HphI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV PleI PpuMI
PshBI RsaI Sau3AI Sau96I ScrFI
TaqI TfiI Tru9I Tsp45I Tsp509I
TspRI TthHB8I XhoII