(Circular) (Six-base) MAPSORT of: pFN21AE3313.seq Check: 7023 from: 1 to: 7246 pFN21AE3313 7246 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5056 278 Size: 53 83 186 936 3520 2468 Fragments arranged by size: 3520 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2826 4589 1937 Size: 889 1763 4594 Fragments arranged by size: 4594 1763 889 Acc65I G'GTAC_C Cuts at: 4567 4567 Size: 7246 AclI AA'CG_TT Cuts at: 1563 2691 5373 5746 1563 Size: 1128 2682 373 3063 Fragments arranged by size: 3063 2682 1128 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2982 3078 5309 6693 6961 1632 Size: 1350 96 2231 1384 268 1917 Fragments arranged by size: 2231 1917 1384 1350 268 96 AflII C'TTAA_G Cuts at: 820 1017 2572 820 Size: 197 1555 5494 Fragments arranged by size: 5494 1555 197 AflIII A'CryG_T Cuts at: 1203 3402 3534 6145 7122 1203 Size: 2199 132 2611 977 1327 Fragments arranged by size: 2611 2199 1327 977 132 AgeI A'CCGG_T Cuts at: 6077 6077 Size: 7246 AhdI GACnn_n'nnGTC Cuts at: 5975 5975 Size: 7246 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7246 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7246 AlwNI CAG_nnn'CTG Cuts at: 2960 3686 4325 4989 6561 2960 Size: 726 639 664 1572 3645 Fragments arranged by size: 3645 1572 726 664 639 ApaLI G'TGCA_C Cuts at: 5303 6459 5303 Size: 1156 6090 ApoI r'AATT_y Cuts at: 1493 2327 2474 2831 2855 3779 3917 3948 4343 Size: 834 147 357 24 924 138 31 395 Cuts at: 4343 4422 4555 4781 4933 7042 1493 Size: 79 133 226 152 2109 1697 Fragments arranged by size: 2109 1697 924 834 395 357 226 152 147 138 133 79 31 24 AseI AT'TA_AT Cuts at: 160 1032 2336 2519 3597 5800 160 Size: 872 1304 183 1078 2203 1606 Fragments arranged by size: 2203 1606 1304 1078 872 183 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7246 AvaI C'yCGr_G Cuts at: 1870 1943 4571 1870 Size: 73 2628 4545 Fragments arranged by size: 4545 2628 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2340 2373 2340 Size: 33 7213 BamHI G'GATC_C Cuts at: 3829 4576 4943 3829 Size: 747 367 6132 Fragments arranged by size: 6132 747 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4567 6022 618 Size: 325 217 627 2780 1455 1842 Fragments arranged by size: 2780 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4565 1391 Size: 3174 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2255 928 Size: 971 356 5919 Fragments arranged by size: 5919 971 356 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5437 5471 1903 Size: 34 3500 34 3678 Fragments arranged by size: 3678 3500 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5138 6348 5138 Size: 1210 6036 BclI T'GATC_A Cuts at: 1548 3397 1548 Size: 1849 5397 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3683 5857 136 Size: 107 122 71 631 605 2011 2174 1525 Fragments arranged by size: 2174 2011 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3889 7241 3889 Size: 3352 3894 BlpI GC'TnA_GC Cuts at: 4663 4663 Size: 7246 Bme1580I G_kGCm'C Cuts at: 1790 5307 6463 1790 Size: 3517 1156 2573 Fragments arranged by size: 3517 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7246 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4447 4977 5935 Size: 416 53 83 399 24 3030 530 958 Cuts at: 5935 442 Size: 1753 Fragments arranged by size: 3030 1753 958 530 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2919 1056 Size: 1863 5383 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2101 5906 1239 Size: 862 3805 2579 Fragments arranged by size: 3805 2579 862 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3135 5371 6236 6534 3135 Size: 2236 865 298 3847 Fragments arranged by size: 3847 2236 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2277 5909 882 Size: 617 778 3632 2219 Fragments arranged by size: 3632 2219 778 617 BsaAI yAC'GTr Cuts at: 493 1204 5037 493 Size: 711 3833 2702 Fragments arranged by size: 3833 2702 711 BsaBI GATnn'nnATC Cuts at: 7240 7240 Size: 7246 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5053 5435 6070 275 Size: 53 83 186 936 3520 382 635 1451 Fragments arranged by size: 3520 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5679 6077 6351 6498 6909 5679 Size: 398 274 147 411 6016 Fragments arranged by size: 6016 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2144 2174 1178 Size: 30 165 30 741 30 6250 Fragments arranged by size: 6250 741 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2165 3279 1186 Size: 979 1114 5153 Fragments arranged by size: 5153 1114 979 BseYI C'CCAG_C Cuts at: 6449 6945 6449 Size: 496 6750 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6673 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4696 5457 6485 1540 Size: 275 186 2695 761 1028 2301 Fragments arranged by size: 2695 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5307 5392 6463 5307 Size: 85 1071 6090 Fragments arranged by size: 6090 1071 85 BsmI GAATG_Cn' Cuts at: 4025 4757 4850 4025 Size: 732 93 6421 Fragments arranged by size: 6421 732 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4565 5307 5392 6463 1391 Size: 399 2775 742 85 1071 2174 Fragments arranged by size: 2775 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6909 6909 Size: 7246 BspHI T'CATG_A Cuts at: 4048 5135 6865 4048 Size: 1087 1730 4429 Fragments arranged by size: 4429 1730 1087 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4601 844 Size: 3757 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5133 1394 Size: 429 3310 3507 Fragments arranged by size: 3507 3310 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2949 3051 5741 5915 65 Size: 1183 1701 102 2690 174 1396 Fragments arranged by size: 2690 1701 1396 1183 174 102 BsrFI r'CCGG_y Cuts at: 1700 1810 5890 6077 1700 Size: 110 4080 187 2869 Fragments arranged by size: 4080 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2141 96 Size: 2045 5201 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7246 BssSI C'ACGA_G Cuts at: 5306 6318 5306 Size: 1012 6234 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7246 BstBI TT'CG_AA Cuts at: 2719 6050 2719 Size: 3331 3915 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7246 BstXI CCAn_nnnn'nTGG Cuts at: 2079 2079 Size: 7246 BstYI r'GATC_y Cuts at: 1972 3496 3705 3829 3889 4576 4943 5328 5345 Size: 1524 209 124 60 687 367 385 17 Cuts at: 5345 6797 6883 6895 7241 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1524 1452 687 385 367 346 209 124 86 60 17 12 BstZ17I GTA'TAC Cuts at: 2827 2827 Size: 7246 BtgI C'CryG_G Cuts at: 513 1065 4040 513 Size: 552 2975 3719 Fragments arranged by size: 3719 2975 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6507 Fragments arranged by size: 6507 683 56 BtsI GCAGTG_nn' Cuts at: 3039 4762 5556 5576 3039 Size: 1723 794 20 4709 Fragments arranged by size: 4709 1723 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7246 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7211 DraI TTT'AAA Cuts at: 4117 4550 4117 Size: 433 6813 DraIII CAC_nnn'GTG Cuts at: 3341 3341 Size: 7246 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6253 809 Size: 507 4937 1802 Fragments arranged by size: 4937 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3864 4693 5582 Size: 54 1425 50 275 18 2034 829 889 Cuts at: 5582 7211 8 Size: 1629 43 Fragments arranged by size: 2034 1629 1425 889 829 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4693 1537 Size: 275 2881 4090 Fragments arranged by size: 4090 2881 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2310 4233 5176 1353 Size: 957 1923 943 3423 Fragments arranged by size: 3423 1923 957 943 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5833 6207 6353 387 Size: 1064 138 4244 374 146 1280 Fragments arranged by size: 4244 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2101 2982 3078 5309 5906 6693 6961 Size: 393 469 881 96 2231 597 787 268 Cuts at: 6961 1239 Size: 1524 Fragments arranged by size: 2231 1524 881 787 597 469 393 268 96 EcoO109I rG'GnC_Cy Cuts at: 2945 2957 3007 2945 Size: 12 50 7184 Fragments arranged by size: 7184 50 12 EcoRI G'AATT_C Cuts at: 4555 4933 4555 Size: 378 6868 EcoRV GAT'ATC Cuts at: 2732 3312 2732 Size: 580 6666 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2207 3108 4353 2207 Size: 901 1245 5100 Fragments arranged by size: 5100 1245 901 FspI TGC'GCA Cuts at: 5752 5752 Size: 7246 HaeII r_GCGC'y Cuts at: 1678 6393 1678 Size: 4715 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3608 3640 5887 5919 5961 Size: 32 135 32 2036 32 2247 32 42 Cuts at: 5961 5993 1373 Size: 32 2626 Fragments arranged by size: 2626 2247 2036 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2599 2689 4064 4482 4590 4836 Size: 988 281 661 90 1375 418 108 246 Cuts at: 4836 669 Size: 3079 Fragments arranged by size: 3079 1375 988 661 418 281 246 108 90 HindIII A'AGCT_T Cuts at: 748 2234 748 Size: 1486 5760 HpaI GTT'AAC Cuts at: 4836 4836 Size: 7246 KpnI G_GTAC'C Cuts at: 4571 4571 Size: 7246 MfeI C'AATT_G Cuts at: 4147 4845 4147 Size: 698 6548 MluI A'CGCG_T Cuts at: 7122 7122 Size: 7246 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4282 6360 6544 1305 Size: 2977 2078 184 2007 Fragments arranged by size: 2977 2078 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3866 10 Size: 54 1425 343 2034 3390 Fragments arranged by size: 3390 2034 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3114 5204 5563 5722 518 Size: 648 1948 2090 359 159 2042 Fragments arranged by size: 2090 2042 1948 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4662 5339 6487 6732 1606 Size: 3056 677 1148 245 2120 Fragments arranged by size: 3056 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 4040 513 Size: 552 2975 3719 Fragments arranged by size: 3719 2975 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7246 NheI G'CTAG_C Cuts at: 1052 2915 1052 Size: 1863 5383 NotI GC'GGCC_GC Cuts at: 4693 4693 Size: 7246 NspI r_CATG'y Cuts at: 1132 1229 3406 4604 6149 1132 Size: 97 2177 1198 1545 2229 Fragments arranged by size: 2229 2177 1545 1198 97 PasI CC'CwG_GG Cuts at: 1348 4278 1348 Size: 2930 4316 PciI A'CATG_T Cuts at: 3402 6145 3402 Size: 2743 4503 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7246 PfoI T'CCnGG_A Cuts at: 2078 2078 Size: 7246 PmeI GTTT'AAAC Cuts at: 4550 4550 Size: 7246 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5315 5347 6828 6860 5315 Size: 32 1481 32 5701 Fragments arranged by size: 5701 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2945 2957 3007 2945 Size: 12 50 7184 Fragments arranged by size: 7184 50 12 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7246 PsiI TTA'TAA Cuts at: 2864 4816 2864 Size: 1952 5294 PstI C_TGCA'G Cuts at: 830 4598 830 Size: 3768 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7246 SalI G'TCGA_C Cuts at: 1936 4588 1936 Size: 2652 4594 SanDI GG'GwC_CC Cuts at: 2945 2945 Size: 7246 SbfI CC_TGCA'GG Cuts at: 4598 4598 Size: 7246 ScaI AGT'ACT Cuts at: 3640 3655 5494 7206 3640 Size: 15 1839 1712 3680 Fragments arranged by size: 3680 1839 1712 15 SfcI C'TryA_G Cuts at: 826 1045 4594 5729 6410 6601 826 Size: 219 3549 1135 681 191 1471 Fragments arranged by size: 3549 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7246 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7246 SmaI CCC'GGG Cuts at: 1872 4573 1872 Size: 2701 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2572 3150 5350 6251 6513 820 Size: 197 926 629 578 2200 901 262 1553 Fragments arranged by size: 2200 1553 926 901 629 578 262 197 SnaBI TAC'GTA Cuts at: 493 5037 493 Size: 4544 2702 SpeI A'CTAG_T Cuts at: 152 2419 152 Size: 2267 4979 SphI G_CATG'C Cuts at: 1132 4604 1132 Size: 3472 3774 SspI AAT'ATT Cuts at: 5 52 3878 5170 5 Size: 47 3826 1292 2081 Fragments arranged by size: 3826 2081 1292 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7246 StyI C'CwwG_G Cuts at: 513 1065 4040 4685 513 Size: 552 2975 645 3074 Fragments arranged by size: 3074 2975 645 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5273 5290 5443 5628 6073 325 Size: 1255 3693 17 153 185 445 1498 Fragments arranged by size: 3693 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2141 3638 3653 Size: 274 80 33 51 1443 164 1497 15 Cuts at: 3653 5492 7204 96 Size: 1839 1712 138 Fragments arranged by size: 1839 1712 1497 1443 274 164 138 80 51 33 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2007 2039 3780 3812 7206 7238 2007 Size: 32 1741 32 3394 32 2015 Fragments arranged by size: 3394 2015 1741 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7246 XbaI T'CTAG_A Cuts at: 4582 4582 Size: 7246 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4275 4275 Size: 7246 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7246 XmaI C'CCGG_G Cuts at: 1870 4571 1870 Size: 2701 4545 XmnI GAAnn'nnTTC Cuts at: 2892 3879 5375 2892 Size: 987 1496 4763 Fragments arranged by size: 4763 1496 987 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5054 276 Size: 53 83 186 936 3520 2468 Fragments arranged by size: 3520 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI