(Linear) (Six-base) MAPSORT of: KIEE3313.seq Check: 9808 from: 1 to: 2541 KIEE3313.orf With 182 enzymes: SgfI * December 27, 2011 16:35 .. AccI GT'mk_AC Cuts at: 0 821 2541 Size: 821 1720 AclI AA'CG_TT Cuts at: 0 686 2541 Size: 686 1855 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 977 1073 2541 Size: 977 96 1468 Fragments arranged by size: 1468 977 96 AflII C'TTAA_G Cuts at: 0 567 2541 Size: 567 1974 AflIII A'CryG_T Cuts at: 0 1397 1529 2541 Size: 1397 132 1012 Fragments arranged by size: 1397 1012 132 AlwNI CAG_nnn'CTG Cuts at: 0 955 1681 2320 2541 Size: 955 726 639 221 Fragments arranged by size: 955 726 639 221 ApoI r'AATT_y Cuts at: 0 322 469 826 850 1774 1912 1943 2338 Size: 322 147 357 24 924 138 31 395 Cuts at: 2338 2417 2541 Size: 79 124 Fragments arranged by size: 924 395 357 322 147 138 124 79 31 24 AseI AT'TA_AT Cuts at: 0 331 514 1592 2541 Size: 331 183 1078 949 Fragments arranged by size: 1078 949 331 183 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 335 368 2541 Size: 335 33 2173 Fragments arranged by size: 2173 335 33 BamHI G'GATC_C Cuts at: 0 1824 2541 Size: 1824 717 BbsI GAAGACnn'nnnn_ Cuts at: 0 250 2541 Size: 250 2291 BclI T'GATC_A Cuts at: 0 1392 2541 Size: 1392 1149 BglI GCCn_nnn'nGGC Cuts at: 0 1678 2541 Size: 1678 863 BglII A'GATC_T Cuts at: 0 1884 2541 Size: 1884 657 BmrI ACTGGGnnnn_n' Cuts at: 0 2442 2541 Size: 2442 99 BmtI G_CTAG'C Cuts at: 0 914 2541 Size: 914 1627 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 96 2541 Size: 96 2445 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1130 2541 Size: 1130 1411 BsaI GGTCTCn'nnnn_ Cuts at: 0 272 2541 Size: 272 2269 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 139 169 2541 Size: 139 30 2372 Fragments arranged by size: 2372 139 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 160 1274 2541 Size: 160 1114 1267 Fragments arranged by size: 1267 1114 160 BsmI GAATG_Cn' Cuts at: 0 2020 2541 Size: 2020 521 BspHI T'CATG_A Cuts at: 0 2043 2541 Size: 2043 498 BsrDI GCAATG_nn' Cuts at: 0 944 1046 2541 Size: 944 102 1495 Fragments arranged by size: 1495 944 102 BsrGI T'GTAC_A Cuts at: 0 136 2541 Size: 136 2405 BstBI TT'CG_AA Cuts at: 0 714 2541 Size: 714 1827 BstXI CCAn_nnnn'nTGG Cuts at: 0 74 2541 Size: 74 2467 BstYI r'GATC_y Cuts at: 0 1491 1700 1824 1884 2541 Size: 1491 209 124 60 657 Fragments arranged by size: 1491 657 209 124 60 BstZ17I GTA'TAC Cuts at: 0 822 2541 Size: 822 1719 BtgI C'CryG_G Cuts at: 0 2035 2541 Size: 2035 506 BtsI GCAGTG_nn' Cuts at: 0 1034 2541 Size: 1034 1507 DraI TTT'AAA Cuts at: 0 2112 2541 Size: 2112 429 DraIII CAC_nnn'GTG Cuts at: 0 1336 2541 Size: 1336 1205 EaeI y'GGCC_r Cuts at: 0 1859 2541 Size: 1859 682 EarI CTCTTCn'nnn_ Cuts at: 0 305 2228 2541 Size: 305 1923 313 Fragments arranged by size: 1923 313 305 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 96 977 1073 2541 Size: 96 881 96 1468 Fragments arranged by size: 1468 881 96 96 EcoO109I rG'GnC_Cy Cuts at: 0 940 952 1002 2541 Size: 940 12 50 1539 Fragments arranged by size: 1539 940 50 12 EcoRV GAT'ATC Cuts at: 0 727 1307 2541 Size: 727 580 1234 Fragments arranged by size: 1234 727 580 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 202 1103 2348 2541 Size: 202 901 1245 193 Fragments arranged by size: 1245 901 202 193 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1603 1635 2541 Size: 1603 32 906 Fragments arranged by size: 1603 906 32 HincII GTy'rAC Cuts at: 0 594 684 2059 2477 2541 Size: 594 90 1375 418 64 Fragments arranged by size: 1375 594 418 90 64 HindIII A'AGCT_T Cuts at: 0 229 2541 Size: 229 2312 MfeI C'AATT_G Cuts at: 0 2142 2541 Size: 2142 399 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2277 2541 Size: 2277 264 MscI TGG'CCA Cuts at: 0 1861 2541 Size: 1861 680 MslI CAynn'nnrTG Cuts at: 0 1109 2541 Size: 1109 1432 NcoI C'CATG_G Cuts at: 0 2035 2541 Size: 2035 506 NheI G'CTAG_C Cuts at: 0 910 2541 Size: 910 1631 NspI r_CATG'y Cuts at: 0 1401 2541 Size: 1401 1140 PasI CC'CwG_GG Cuts at: 0 2273 2541 Size: 2273 268 PciI A'CATG_T Cuts at: 0 1397 2541 Size: 1397 1144 PfoI T'CCnGG_A Cuts at: 0 73 2541 Size: 73 2468 PpuMI rG'GwC_Cy Cuts at: 0 940 952 1002 2541 Size: 940 12 50 1539 Fragments arranged by size: 1539 940 50 12 PsiI TTA'TAA Cuts at: 0 859 2541 Size: 859 1682 SanDI GG'GwC_CC Cuts at: 0 940 2541 Size: 940 1601 ScaI AGT'ACT Cuts at: 0 1635 1650 2541 Size: 1635 15 891 Fragments arranged by size: 1635 891 15 SmlI C'TyrA_G Cuts at: 0 567 1145 2541 Size: 567 578 1396 Fragments arranged by size: 1396 578 567 SpeI A'CTAG_T Cuts at: 0 414 2541 Size: 414 2127 SspI AAT'ATT Cuts at: 0 1873 2541 Size: 1873 668 StyI C'CwwG_G Cuts at: 0 2035 2541 Size: 2035 506 TatI w'GTAC_w Cuts at: 0 136 1633 1648 2541 Size: 136 1497 15 893 Fragments arranged by size: 1497 893 136 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2 34 1775 1807 2541 Size: 2 32 1741 32 734 Fragments arranged by size: 1741 734 32 32 2 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2270 2541 Size: 2270 271 XmnI GAAnn'nnTTC Cuts at: 0 887 1874 2541 Size: 887 987 667 Fragments arranged by size: 987 887 667 Enzymes that do cut: AccI AclI AcuI AflII AflIII AlwNI ApoI AseI BaeI BamHI BbsI BclI BglI BglII BmrI BmtI BpmI BpuEI BsaI BsaXI BseRI BsmI BspHI BsrDI BsrGI BstBI BstXI BstYI BstZ17I BtgI BtsI DraI DraIII EaeI EarI Eco57MI EcoO109I EcoRV FalI Hin4I HincII HindIII MfeI MmeI MscI MslI NcoI NheI NspI PasI PciI PfoI PpuMI PsiI SanDI ScaI SmlI SpeI SspI StyI TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AfeI AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII BanI BanII BbeI BbvCI BcgI BciVI BfrBI BlpI Bme1580I BmgBI BplI Bpu10I BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstEII Bsu36I BtgZI ClaI CspCI DrdI EagI EciI EcoICRI EcoNI EcoRI FseI FspI FspAI HaeII HpaI KasI KpnI MluI MspA1I NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PflMI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI StuI SwaI TaqII Tth111I XbaI XhoI XmaI ZraI