(Circular) (Six-base) MAPSORT of: pFN21AE0028.seq Check: 1647 from: 1 to: 6946 pFN21AE0028 6946 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2155 2155 Size: 6946 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2921 4756 278 Size: 53 83 186 936 1385 1835 2468 Fragments arranged by size: 2468 1835 1385 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2295 3825 4289 1937 Size: 358 1530 464 4594 Fragments arranged by size: 4594 1530 464 358 Acc65I G'GTAC_C Cuts at: 4267 4267 Size: 6946 AclI AA'CG_TT Cuts at: 1563 5073 5446 1563 Size: 3510 373 3063 Fragments arranged by size: 3510 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2206 2651 5009 6393 6661 1632 Size: 574 445 2358 1384 268 1917 Fragments arranged by size: 2358 1917 1384 574 445 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6749 AflIII A'CryG_T Cuts at: 1203 4052 5845 6822 1203 Size: 2849 1793 977 1327 Fragments arranged by size: 2849 1793 1327 977 AgeI A'CCGG_T Cuts at: 5777 5777 Size: 6946 AhdI GACnn_n'nnGTC Cuts at: 2917 5675 2917 Size: 2758 4188 AleI CACnn'nnGTG Cuts at: 1166 3909 1166 Size: 2743 4203 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3647 1184 Size: 2463 4483 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2105 2137 3869 3901 2105 Size: 32 1732 32 5150 Fragments arranged by size: 5150 1732 32 32 AlwNI CAG_nnn'CTG Cuts at: 2522 3092 3158 4689 6261 2522 Size: 570 66 1531 1572 3207 Fragments arranged by size: 3207 1572 1531 570 66 ApaI G_GGCC'C Cuts at: 2849 3811 3959 2849 Size: 962 148 5836 Fragments arranged by size: 5836 962 148 ApaLI G'TGCA_C Cuts at: 5003 6159 5003 Size: 1156 5790 ApoI r'AATT_y Cuts at: 1493 4255 4481 4633 6742 1493 Size: 2762 226 152 2109 1697 Fragments arranged by size: 2762 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5500 160 Size: 872 4468 1606 Fragments arranged by size: 4468 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6946 AvaI C'yCGr_G Cuts at: 1870 1943 3138 3179 4271 1870 Size: 73 1195 41 1092 4545 Fragments arranged by size: 4545 1195 1092 73 41 AvrII C'CTAG_G Cuts at: 4123 4123 Size: 6946 BamHI G'GATC_C Cuts at: 3679 4276 4643 3679 Size: 597 367 5982 Fragments arranged by size: 5982 597 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2897 2997 4267 5722 618 Size: 325 217 627 1110 100 1270 1455 1842 Fragments arranged by size: 1842 1455 1270 1110 627 325 217 100 BanII G_rGCy'C Cuts at: 1391 2822 2849 2867 3099 3111 3811 3959 4141 Size: 1431 27 18 232 12 700 148 182 Cuts at: 4141 4265 1391 Size: 124 4072 Fragments arranged by size: 4072 1431 700 232 182 148 124 27 18 12 BbeI G_GCGC'C Cuts at: 3001 3001 Size: 6946 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5975 BbvCI CC'TCA_GC Cuts at: 2948 2948 Size: 6946 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5137 5171 1903 Size: 34 3200 34 3678 Fragments arranged by size: 3678 3200 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3934 3968 4838 6048 3934 Size: 34 870 1210 4832 Fragments arranged by size: 4832 1210 870 34 BclI T'GATC_A Cuts at: 1548 1548 Size: 6946 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4128 5557 136 Size: 107 122 71 631 605 2456 1429 1525 Fragments arranged by size: 2456 1525 1429 631 605 122 107 71 BglII A'GATC_T Cuts at: 6941 6941 Size: 6946 BlpI GC'TnA_GC Cuts at: 3093 4363 3093 Size: 1270 5676 Bme1580I G_kGCm'C Cuts at: 1790 2849 2900 3811 3959 5007 6163 1790 Size: 1059 51 911 148 1048 1156 2573 Fragments arranged by size: 2573 1156 1059 1048 911 148 51 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6946 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2761 2973 3046 Size: 416 53 83 399 24 1344 212 73 Cuts at: 3046 4677 5635 442 Size: 1631 958 1753 Fragments arranged by size: 1753 1631 1344 958 416 399 212 83 73 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6946 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3052 3844 3052 Size: 792 6154 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2345 3650 3823 4127 4180 5606 1239 Size: 1106 1305 173 304 53 1426 2579 Fragments arranged by size: 2579 1426 1305 1106 304 173 53 Bpu10I CC'TnA_GC Cuts at: 2328 2492 2841 2948 2328 Size: 164 349 107 6326 Fragments arranged by size: 6326 349 164 107 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2809 3902 4138 5071 5936 6234 2809 Size: 1093 236 933 865 298 3521 Fragments arranged by size: 3521 1093 933 865 298 236 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5609 882 Size: 617 4110 2219 Fragments arranged by size: 4110 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4737 493 Size: 711 3533 2702 Fragments arranged by size: 3533 2702 711 BsaBI GATnn'nnATC Cuts at: 6940 6940 Size: 6946 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2091 2918 2998 3756 Size: 53 83 186 936 558 827 80 758 Cuts at: 3756 4071 4753 5135 5770 275 Size: 315 682 382 635 1451 Fragments arranged by size: 1451 936 827 758 682 635 558 382 315 186 83 80 53 BsaWI w'CCGG_w Cuts at: 2921 2972 5379 5777 6051 6198 6609 2921 Size: 51 2407 398 274 147 411 3258 Fragments arranged by size: 3258 2407 411 398 274 147 51 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2105 2135 1178 Size: 30 165 30 702 30 5989 Fragments arranged by size: 5989 702 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2654 2841 2936 3183 3224 3280 1186 Size: 1468 187 95 247 41 56 4852 Fragments arranged by size: 4852 1468 247 187 95 56 41 BseYI C'CCAG_C Cuts at: 3199 3406 4084 6149 6645 3199 Size: 207 678 2065 496 3500 Fragments arranged by size: 3500 2065 678 496 207 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2465 2761 3078 3532 1163 Size: 573 729 296 317 454 4577 Fragments arranged by size: 4577 729 573 454 317 296 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4396 5157 6185 1540 Size: 275 186 2395 761 1028 2301 Fragments arranged by size: 2395 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2867 3111 4166 5007 5092 6163 2867 Size: 244 1055 841 85 1071 3650 Fragments arranged by size: 3650 1071 1055 841 244 85 BsmI GAATG_Cn' Cuts at: 2758 4457 4550 2758 Size: 1699 93 5154 Fragments arranged by size: 5154 1699 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3437 3437 Size: 6946 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2822 2849 2867 2900 3099 3111 3811 Size: 399 1032 27 18 33 199 12 700 Cuts at: 3811 3959 4141 4166 4265 5007 5092 6163 1391 Size: 148 182 25 99 742 85 1071 2174 Fragments arranged by size: 2174 1071 1032 742 700 399 199 182 148 99 85 33 27 25 18 12 BspEI T'CCGG_A Cuts at: 2921 6609 2921 Size: 3688 3258 BspHI T'CATG_A Cuts at: 4835 6565 4835 Size: 1730 5216 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2058 2155 3738 3982 4193 4301 844 Size: 1214 97 1583 244 211 108 3489 Fragments arranged by size: 3489 1583 1214 244 211 108 97 BsrBI CCG'CTC Cuts at: 1394 1823 4833 1394 Size: 429 3010 3507 Fragments arranged by size: 3507 3010 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2606 3690 5441 5615 65 Size: 1183 1358 1084 1751 174 1396 Fragments arranged by size: 1751 1396 1358 1183 1084 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2988 5590 5777 1700 Size: 110 1178 2602 187 2869 Fragments arranged by size: 2869 2602 1178 187 110 BsrGI T'GTAC_A Cuts at: 96 3704 3861 96 Size: 3608 157 3181 Fragments arranged by size: 3608 3181 157 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6946 BssSI C'ACGA_G Cuts at: 2775 5006 6018 2775 Size: 2231 1012 3703 Fragments arranged by size: 3703 2231 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2077 2161 794 Size: 1283 84 5579 Fragments arranged by size: 5579 1283 84 BstBI TT'CG_AA Cuts at: 5750 5750 Size: 6946 BstEII G'GTnAC_C Cuts at: 1184 4147 1184 Size: 2963 3983 BstXI CCAn_nnnn'nTGG Cuts at: 3911 4231 3911 Size: 320 6626 BstYI r'GATC_y Cuts at: 1972 3679 4276 4643 5028 5045 6497 6583 6595 Size: 1707 597 367 385 17 1452 86 12 Cuts at: 6595 6941 1972 Size: 346 1977 Fragments arranged by size: 1977 1707 1452 597 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3159 3159 Size: 6946 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 2059 2352 2733 3010 3951 Size: 552 939 12 43 293 381 277 941 Cuts at: 3951 4152 4170 4200 513 Size: 201 18 30 3259 Fragments arranged by size: 3259 941 939 552 381 293 277 201 43 30 18 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3809 4107 487 Size: 683 56 2583 298 3326 Fragments arranged by size: 3326 2583 683 298 56 BtsI GCAGTG_nn' Cuts at: 2075 3241 3418 4164 4462 5256 5276 2075 Size: 1166 177 746 298 794 20 3745 Fragments arranged by size: 3745 1166 794 746 298 177 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6946 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4069 4104 529 Size: 35 3505 35 3371 Fragments arranged by size: 3505 3371 35 35 DraI TTT'AAA Cuts at: 4250 4250 Size: 6946 DraIII CAC_nnn'GTG Cuts at: 2777 2777 Size: 6946 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2036 5953 809 Size: 507 720 3917 1802 Fragments arranged by size: 3917 1802 720 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2868 2958 4129 Size: 54 1425 50 275 18 1038 90 1171 Cuts at: 4129 4393 5282 6911 8 Size: 264 889 1629 43 Fragments arranged by size: 1629 1425 1171 1038 889 275 264 90 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4393 1537 Size: 275 2581 4090 Fragments arranged by size: 4090 2581 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2060 2225 2649 2655 2810 2933 4022 4876 Size: 707 165 424 6 155 123 1089 854 Cuts at: 4876 1353 Size: 3423 Fragments arranged by size: 3423 1089 854 707 424 165 155 123 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2120 3164 5533 5907 6053 Size: 1064 138 435 96 1044 2369 374 146 Cuts at: 6053 387 Size: 1280 Fragments arranged by size: 2369 1280 1064 1044 435 374 146 138 96 EcoICRI GAG'CTC Cuts at: 2865 3109 2865 Size: 244 6702 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2206 2345 2651 3650 3823 4127 4180 Size: 393 574 139 306 999 173 304 53 Cuts at: 4180 5009 5606 6393 6661 1239 Size: 829 597 787 268 1524 Fragments arranged by size: 1524 999 829 787 597 574 393 306 304 268 173 139 53 EcoO109I rG'GnC_Cy Cuts at: 2845 2846 2911 3155 3525 3807 3956 4182 2845 Size: 1 65 244 370 282 149 226 5609 Fragments arranged by size: 5609 370 282 244 226 149 65 1 EcoRI G'AATT_C Cuts at: 4255 4633 4255 Size: 378 6568 FspI TGC'GCA Cuts at: 5452 5452 Size: 6946 HaeII r_GCGC'y Cuts at: 1678 3001 6093 1678 Size: 1323 3092 2531 Fragments arranged by size: 3092 2531 1323 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2238 2270 2903 2935 5587 Size: 32 135 32 666 32 633 32 2652 Cuts at: 5587 5619 5661 5693 1373 Size: 32 42 32 2626 Fragments arranged by size: 2652 2626 666 633 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4290 4536 669 Size: 988 281 2352 246 3079 Fragments arranged by size: 3079 2352 988 281 246 HindIII A'AGCT_T Cuts at: 748 2597 748 Size: 1849 5097 HpaI GTT'AAC Cuts at: 4536 4536 Size: 6946 KasI G'GCGC_C Cuts at: 2997 2997 Size: 6946 KpnI G_GTAC'C Cuts at: 4271 4271 Size: 6946 MfeI C'AATT_G Cuts at: 4545 4545 Size: 6946 MluI A'CGCG_T Cuts at: 6822 6822 Size: 6946 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2698 2724 6060 6244 1305 Size: 1393 26 3336 184 2007 Fragments arranged by size: 3336 2007 1393 184 26 MscI TGG'CCA Cuts at: 10 64 1489 1832 2960 4131 10 Size: 54 1425 343 1128 1171 2825 Fragments arranged by size: 2825 1425 1171 1128 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3302 3909 4169 4904 5263 5422 518 Size: 648 2136 607 260 735 359 159 2042 Fragments arranged by size: 2136 2042 735 648 607 359 260 159 MspA1I CmG'CkG Cuts at: 1606 2061 2086 2149 2952 2995 3361 3749 3981 Size: 455 25 63 803 43 366 388 232 Cuts at: 3981 4362 5039 6187 6432 1606 Size: 381 677 1148 245 2120 Fragments arranged by size: 2120 1148 803 677 455 388 381 366 245 232 63 43 25 NarI GG'CG_CC Cuts at: 2998 2998 Size: 6946 NcoI C'CATG_G Cuts at: 513 1065 2004 2733 3010 4170 4200 513 Size: 552 939 729 277 1160 30 3259 Fragments arranged by size: 3259 1160 939 729 552 277 30 NdeI CA'TA_TG Cuts at: 387 387 Size: 6946 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6946 NotI GC'GGCC_GC Cuts at: 4393 4393 Size: 6946 NspI r_CATG'y Cuts at: 1132 1229 2515 3490 3778 4048 4056 4304 5849 Size: 97 1286 975 288 270 8 248 1545 Cuts at: 5849 1132 Size: 2229 Fragments arranged by size: 2229 1545 1286 975 288 270 248 97 8 PasI CC'CwG_GG Cuts at: 1348 4005 1348 Size: 2657 4289 PciI A'CATG_T Cuts at: 4052 5845 4052 Size: 1793 5153 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6946 PfoI T'CCnGG_A Cuts at: 2040 2040 Size: 6946 PmeI GTTT'AAAC Cuts at: 4250 4250 Size: 6946 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2105 2137 5015 5047 6528 6560 2105 Size: 32 2878 32 1481 32 2491 Fragments arranged by size: 2878 2491 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2911 4182 2911 Size: 1271 5675 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6946 PsiI TTA'TAA Cuts at: 4516 4516 Size: 6946 PspOMI G'GGCC_C Cuts at: 2845 3807 3955 2845 Size: 962 148 5836 Fragments arranged by size: 5836 962 148 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3845 3877 3845 Size: 32 6914 PstI C_TGCA'G Cuts at: 830 2154 3006 3248 4298 830 Size: 1324 852 242 1050 3478 Fragments arranged by size: 3478 1324 1050 852 242 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6946 PvuII CAG'CTG Cuts at: 2086 2149 3749 2086 Size: 63 1600 5283 Fragments arranged by size: 5283 1600 63 SacI G_AGCT'C Cuts at: 2867 3111 2867 Size: 244 6702 SacII CC_GC'GG Cuts at: 2062 2062 Size: 6946 SalI G'TCGA_C Cuts at: 1936 4288 1936 Size: 2352 4594 SapI GCTCTTCn'nnn_ Cuts at: 2060 2225 2810 2933 2060 Size: 165 585 123 6073 Fragments arranged by size: 6073 585 165 123 SbfI CC_TGCA'GG Cuts at: 3006 4298 3006 Size: 1292 5654 ScaI AGT'ACT Cuts at: 5194 6906 5194 Size: 1712 5234 SexAI A'CCwGG_T Cuts at: 2562 2562 Size: 6946 SfcI C'TryA_G Cuts at: 826 1045 2150 3002 3244 4294 5429 6110 6301 Size: 219 1105 852 242 1050 1135 681 191 Cuts at: 6301 826 Size: 1471 Fragments arranged by size: 1471 1135 1105 1050 852 681 242 219 191 SfoI GGC'GCC Cuts at: 2999 2999 Size: 6946 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6946 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6946 SmaI CCC'GGG Cuts at: 1872 4273 1872 Size: 2401 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2824 3917 4117 5050 5951 6213 Size: 197 926 881 1093 200 933 901 262 Cuts at: 6213 820 Size: 1553 Fragments arranged by size: 1553 1093 933 926 901 881 262 200 197 SnaBI TAC'GTA Cuts at: 493 4737 493 Size: 4244 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6946 SphI G_CATG'C Cuts at: 1132 2515 4304 1132 Size: 1383 1789 3774 Fragments arranged by size: 3774 1789 1383 SspI AAT'ATT Cuts at: 5 52 4870 5 Size: 47 4818 2081 Fragments arranged by size: 4818 2081 47 StuI AGG'CCT Cuts at: 1517 2666 1517 Size: 1149 5797 StyI C'CwwG_G Cuts at: 513 1065 2004 2667 2733 2901 2928 3010 4123 Size: 552 939 663 66 168 27 82 1113 Cuts at: 4123 4170 4200 4385 513 Size: 47 30 185 3074 Fragments arranged by size: 3074 1113 939 663 552 185 168 82 66 47 30 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4076 4973 4990 5143 5328 5773 325 Size: 1255 2496 897 17 153 185 445 1498 Fragments arranged by size: 2496 1498 1255 897 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3704 3861 3930 Size: 274 80 33 51 1443 1727 157 69 Cuts at: 3930 5192 6904 96 Size: 1262 1712 138 Fragments arranged by size: 1727 1712 1443 1262 274 157 138 80 69 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3215 3247 6906 6938 3215 Size: 32 3659 32 3223 Fragments arranged by size: 3659 3223 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6946 XbaI T'CTAG_A Cuts at: 4282 4282 Size: 6946 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6946 XmaI C'CCGG_G Cuts at: 1870 4271 1870 Size: 2401 4545 XmnI GAAnn'nnTTC Cuts at: 2120 5075 2120 Size: 2955 3991 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2919 4754 276 Size: 53 83 186 936 1385 1835 2468 Fragments arranged by size: 2468 1835 1385 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BfrBI BsiWI BstZ17I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SanDI SfiI SrfI SwaI XcmI