Restriction map for
pFN21AB6409
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspEI BspHI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I CpoI
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 6 CGCG CG^CG
AcsI 172 AAATTT [GA]^AATT[CT]
AfaI 822 GTAC GT^AC
Alw44I 650 GTGCAC G^TGCAC
AlwNI 491 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 650 GTGCAC G^TGCAC
ApoI 172 AAATTT [GA]^AATT[CT]
BanII 812 GAGCTC G[GA]GC[CT]^C
BbsI 799 GAAGAC GAAGAC(2/6)
BclI 341 TGATCA T^GATCA
BcnI 646 CCGGG CC^[GC]GG
BfaI 809 CTAG C^TAG
BpuAI 799 GAAGAC GAAGAC(2/6)
BsaI 89 GAGACC GGTCTC(1/5)
BseRI 482 CTCCTC GAGGAG(10/8)
BspDI 145 ATCGAT AT^CGAT
BspMI 738 ACCTGC ACCTGC(4/8)
BsrBI 25 CCGCTC CCGCTC(-3/-3)
BsrDI 124 GCAATG GCAATG(2/0)
BstNI 774 CCAGG CC^[AT]GG
BstUI 6 CGCG CG^CG
CfoI 7 GCGC GCG^C
Cfr13I 493 GGCCC G^G[ACGT]CC
ClaI 145 ATCGAT AT^CGAT
DraI 465 TTTAAA TTT^AAA
DraII 492 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 2 TGGCCG [CT]^GGCC[GA]
EcoNI 484 CCTCAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 492 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRII 774 CCAGG ^CC[AT]GG
FbaI 341 TGATCA T^GATCA
HapII 646 CCGG C^CGG
HhaI 7 GCGC GCG^C
HinP1I 7 GCGC G^CGC
HpaII 646 CCGG C^CGG
HphI 223 GGTGA GGTGA(8/7)
MaeI 809 CTAG C^TAG
MaeIII 900 GTTAC ^GT[ACGT]AC
MspA1I 16 CCGCTG C[AC]G^C[GT]G
MspI 646 CCGG C^CGG
MvaI 774 CCAGG CC^[AT]GG
MvnI 6 CGCG CG^CG
NciI 646 CCGGG CC^[GC]GG
NspI 608 ACATGC [GA]CATG^[CT]
PleI 480 GACTC GAGTC(4/5)
RsaI 822 GTAC GT^AC
SacI 812 GAGCTC GAGCT^C
Sau96I 493 GGCCC G^G[ACGT]CC
ScaI 821 AGTACT AGT^ACT
TaqI 146 TCGA T^CGA
TthHB8I 146 TCGA T^CGA
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwI 748 GATCC GGATC(4/5)
192 GGATC
AspHI 650 GTGCAC G[AT]GC[AT]^C
812 GAGCTC
BbvI 547 GCAGC GCAGC(8/12)
18 GCTGC
BpmI 659 CTCCAG CTGGAG(16/14)
542 CTGGAG
BsaJI 773 CCCAGG C^C[ACGT][ACGT]GG
774 CCAGGG
BsiHKAI 650 GTGCAC G[AT]GC[AT]^C
812 GAGCTC
BsiYI 93 CCCTGTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
484 CCTCAAGCAGG
BslI 93 CCCTGTGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
484 CCTCAAGCAGG
BsmAI 89 GAGAC GTCTC(1/5)
478 GAGAC
BsrI 133 ACTGG ACTGG(1/-1)
628 ACTGG
BssKI 646 CCGGG ^CC[ACGT]GG
774 CCAGG
BstF5I 453 CATCC GGATG(2/0)
780 GGATG
FokI 453 CATCC GGATG(9/13)
780 GGATG
HaeIII 3 GGCC GG^CC
493 GGCC
MseI 187 TTAA T^TAA
466 TTAA
ScrFI 646 CCGGG CC^[ACGT]GG
774 CCAGG
SfaNI 538 GATGC GCATC(5/9)
54 GCATC
TfiI 639 GATTC G^A[AT]TC
879 GAATC
Tru9I 187 TTAA T^TAA
466 TTAA
TseI 18 GCTGC G^C[AT]GC
547 GCAGC
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
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AciI AluI BmyI Bsp1286I Cac8I
DdeI DpnII FauI Fnu4HI HinfI
ItaI MboI MboII MnlI MwoI
NdeII NlaIII Sau3AI SmlI Tsp509I
TspRI