################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AspI AvaI AviII AvrII BbeI BbsI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstZ17I CelII CfoI ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI KasI Ksp632I KspI MaeI MaeIII MamI MfeI MluI MroI MseI MspA1I MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 167 GGTACC G^GTACC Asp718I 167 GGTACC G^GTACC BalI 278 TGGCCA TGG^CCA BamHI 265 GGATCC G^GATCC BanI 167 GGTACC G^G[CT][GA]CC BbrPI 425 CACGTG CAC^GTG BbvCI 55 CCTCAGC CCTCAGC(-5/-2) BcnI 181 CCGGG CC^[GC]GG BsaAI 425 CACGTG [CT]AC^GT[GA] BseRI 373 GAGGAG GAGGAG(10/8) Bsp1407I 152 TGTACA T^GTACA BspMI 118 ACCTGC ACCTGC(4/8) BsrGI 152 TGTACA T^GTACA BsrI 164 ACTGG ACTGG(1/-1) BstXI 269 CCAAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 265 GGATCC [GA]^GATC[CT] DpnII 266 GATC ^GATC EcoT14I 96 CCTTGG C^C[AT][AT]GG FauI 356 CCCGC CCCGC(4/6) HgaI 158 GCGTC GACGC(5/10) HphI 116 TCACC GGTGA(8/7) KpnI 167 GGTACC GGTAC^C MaeII 426 ACGT A^CGT MboI 266 GATC ^GATC MboII 397 GAAGA GAAGA(8/7) MflI 265 GGATCC [GA]^GATC[CT] MluNI 278 TGGCCA TGG^CCA MscI 278 TGGCCA TGG^CCA MslI 144 CATCGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 78 GCCGGC GCC^GGC NciI 181 CCGGG CC^[GC]GG NdeII 266 GATC ^GATC NgoMIV 78 GCCGGC G^CCGGC PmaCI 425 CACGTG CAC^GTG PmlI 425 CACGTG CAC^GTG PpuMI 245 AGGTCCC [GA]G^G[AT]CC[CT] PshAI 423 GACACGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Sau3AI 266 GATC ^GATC SspBI 152 TGTACA T^GTACA StyI 96 CCTTGG C^C[AT][AT]GG TspRI 411 CACTG CA[GC]TG[ACGT][ACGT]^ XhoII 265 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 34 GTCTAC GT^[AC][GT]AC 160 GTCTAC AfaI 153 GTAC GT^AC 168 GTAC AflIII 424 ACACGT A^C[GA][CT]GT 426 ACGTGT AluI 311 AGCT AG^CT 361 AGCT AlwI 266 GATCC GGATC(4/5) 265 GGATC AocI 126 CCTGAGG CC^T[ACGT]AGG 319 CCTGAGG ApaI 237 GGGCCC GGGCC^C 353 GGGCCC AvaII 52 GGTCC G^G[AT]CC 246 GGTCC BsgI 39 CTGCAC GTGCAG(16/14) 120 CTGCAC BsiYI 281 CCAGGAACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 357 CCGCAGCTCGG BslI 281 CCAGGAACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 357 CCGCAGCTCGG BsmFI 247 GTCCC GGGAC(10/14) 430 GTCCC BsrFI 60 GCCGGT [GA]^CCGG[CT] 78 GCCGGC Bsu36I 126 CCTGAGG CC^T[ACGT]AGG 319 CCTGAGG Cac8I 78 GCCGGC GC[ACGT]^[ACGT]GC 355 GCCCGC Cfr10I 60 GCCGGT [GA]^CCGG[CT] 78 GCCGGC EaeI 80 CGGCCA [CT]^GGCC[GA] 278 TGGCCA Eco81I 126 CCTGAGG CC^T[ACGT]AGG 319 CCTGAGG PstI 313 CTGCAG CTGCA^G 413 CTGCAG RsaI 153 GTAC GT^AC 168 GTAC SfaNI 69 GCATC GCATC(5/9) 302 GCATC SfcI 313 CTGCAG C^T[GA][CT]AG 413 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AciI BanII BbvI BmyI BsaJI Bsp1286I BssKI BstNI Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI HaeIII HapII HpaII ItaI MnlI MspI MvaI MwoI NlaIII NlaIV Sau96I ScrFI TseI