Restriction map for
pFN21AE0149
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstF5I BstPI BstXI
BstYI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DpnII DraI DraIII
DrdI Eam1105I EarI Eco47III EcoO65I
EcoRI EcoRV EcoT22I FbaI FokI
FseI FspI HaeII HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HphI KasI KpnI Ksp632I KspI
MaeI MaeII MamI MboI MfeI
MflI MluI MroI MseI MslI
MunI NaeI NarI NcoI NdeI
NdeII NgoMIV NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tru9I Tsp509I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccII 148 CGCG CG^CG
AocI 193 CCTGAGG CC^T[ACGT]AGG
AspHI 247 GAGCTC G[AT]GC[AT]^C
AvaI 21 CTCGGG C^[CT]CG[GA]G
BalI 113 TGGCCA TGG^CCA
BbsI 99 GAAGAC GAAGAC(2/6)
BbvCI 253 CCTCAGC CCTCAGC(-5/-2)
BcnI 220 CCCGG CC^[GC]GG
BpmI 328 CTGGAG CTGGAG(16/14)
BpuAI 99 GAAGAC GAAGAC(2/6)
BsiEI 179 CGGCCG CG[GA][CT]^CG
BsiHKAI 247 GAGCTC G[AT]GC[AT]^C
BsmAI 144 GAGAC GTCTC(1/5)
BsmBI 144 GAGACG CGTCTC(1/5)
BsoBI 21 CTCGGG C^[CT]CG[GA]G
BsrBI 204 GAGCGG CCGCTC(-3/-3)
BssSI 259 CACGAG CACGAG(-5/-1)
BstAPI 258 GCACGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 148 CGCG CG^CG
Bsu36I 193 CCTGAGG CC^T[ACGT]AGG
DsaI 41 CCACGG C^C[GA][CT]GG
EagI 179 CGGCCG C^GGCCG
EclXI 179 CGGCCG C^GGCCG
Eco52I 179 CGGCCG C^GGCCG
Eco81I 193 CCTGAGG CC^T[ACGT]AGG
EcoNI 309 CCTGAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 146 GACGC GACGC(5/10)
MluNI 113 TGGCCA TGG^CCA
MscI 113 TGGCCA TGG^CCA
MspA1I 212 CAGCGG C[AC]G^C[GT]G
MvnI 148 CGCG CG^CG
NciI 220 CCCGG CC^[GC]GG
SacI 247 GAGCTC GAGCT^C
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AvaII 25 GGACC G^G[AT]CC
293 GGACC
BanI 189 GGCACC G^G[CT][GA]CC
216 GGCACC
BanII 78 GAGCCC G[GA]GC[CT]^C
247 GAGCTC
BbvI 163 GCTGC GCAGC(8/12)
264 GCTGC
BglI 40 GCCACGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
181 GCCGTGACGGC
BmyI 78 GAGCCC G[AGT]GC[ACT]^C
247 GAGCTC
BseRI 66 CTCCTC GAGGAG(10/8)
274 GAGGAG
BsiYI 309 CCTGAGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
384 CCAGGTGGAGG
BslI 309 CCTGAGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
384 CCAGGTGGAGG
BsmFI 24 GGGAC GGGAC(10/14)
292 GGGAC
Bsp1286I 78 GAGCCC G[AGT]GC[ACT]^C
247 GAGCTC
BsrI 83 CCAGT ACTGG(1/-1)
280 CCAGT
BstNI 243 CCAGG CC^[AT]GG
384 CCAGG
DraII 24 GGGACCT [GA]G^G[ACGT]CC[CT]
292 GGGACCC
EcoO109I 24 GGGACCT [GA]G^G[ACGT]CC[CT]
292 GGGACCC
EcoRII 243 CCAGG ^CC[AT]GG
384 CCAGG
EcoT14I 35 CCAAGG C^C[AT][AT]GG
107 CCAAGG
FauI 149 GCGGG CCCGC(4/6)
206 GCGGG
MaeIII 86 GTGAC ^GT[ACGT]AC
184 GTGAC
MvaI 243 CCAGG CC^[AT]GG
384 CCAGG
PpuMI 24 GGGACCT [GA]G^G[AT]CC[CT]
292 GGGACCC
StyI 35 CCAAGG C^C[AT][AT]GG
107 CCAAGG
TseI 163 GCTGC G^C[AT]GC
264 GCTGC
Tsp45I 86 GTGAC ^GT[GC]AC
184 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AluI BsaJI BssKI Cac8I
Cfr13I DdeI EaeI Fnu4HI HaeIII
HapII HinfI HpaII ItaI MboII
MnlI MspI MwoI NlaIV PleI
Sau96I ScrFI TspRI