Restriction map for
pFN21AB7314
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# NO CUT ENZYMES ( 94 enzymes) #
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AatII Acc65I AccIII AflII AflIII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspI AvaI BbrPI
BcgI BciVI BfrI BglI BlpI
Bpu1102I BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsoBI BspDI BspEI
BspLU11I BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
Cfr10I ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV FauI FseI HindIII HpaI
KpnI KspI MamI MluI MroI
NaeI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I StuI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccII 85 CGCG CG^CG
AclI 1136 AACGTT AA^CGTT
AcyI 187 GGCGCC G[GA]^CG[CT]C
AhdI 535 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 2157 ATTAAT AT^TAAT
AsnI 2157 ATTAAT AT^TAAT
Asp700I 694 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 535 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 10 TGCGCA TGC^GCA
AvrII 1062 CCTAGG C^CTAGG
BamHI 2877 GGATCC G^GATCC
BbeI 187 GGCGCC GGCGC^C
BbsI 198 GAAGAC GAAGAC(2/6)
BclI 2274 TGATCA T^GATCA
BcnI 3233 CCCGG CC^[GC]GG
BlnI 1062 CCTAGG C^CTAGG
BpuAI 198 GAAGAC GAAGAC(2/6)
BsaAI 2750 TACGTG [CT]AC^GT[GA]
BsaHI 187 GGCGCC G[GA]^CG[CT]C
BseRI 97 GAGGAG GAGGAG(10/8)
BsmFI 2082 GGGAC GGGAC(10/14)
BspHI 177 TCATGA T^CATGA
BsrBI 1763 CCGCTC CCGCTC(-3/-3)
BstAPI 325 GCATTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 85 CGCG CG^CG
DraII 1803 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1475 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 535 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1803 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 1412 GAATTC G^AATTC
FbaI 2274 TGATCA T^GATCA
FspI 10 TGCGCA TGC^GCA
HapII 3234 CCGG C^CGG
HgaI 1502 GCGTC GACGC(5/10)
Hin1I 187 GGCGCC G[GA]^CG[CT]C
HincII 466 GTTGAC GT[CT]^[GA]AC
HindII 466 GTTGAC GT[CT]^[GA]AC
HpaII 3234 CCGG C^CGG
KasI 187 GGCGCC G^GCGCC
MspI 3234 CCGG C^CGG
MvnI 85 CGCG CG^CG
NarI 187 GGCGCC GG^CGCC
NciI 3233 CCCGG CC^[GC]GG
NheI 2458 GCTAGC G^CTAGC
PpuMI 1803 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 148 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2157 ATTAAT AT^TAAT
Psp1406I 1136 AACGTT AA^CGTT
RcaI 177 TCATGA T^CATGA
SapI 1732 GAAGAGC GCTCTTC(1/4)
SfoI 187 GGCGCC GGC^GCC
SmlI 1094 CTCAAG C^T[CT][GA]AG
SspI 2245 AATATT AAT^ATT
SwaI 1310 ATTTAAAT ATTT^AAAT
XcmI 2204 CCAATGGCAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 694 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 406 GTCTAC GT^[AC][GT]AC
2443 GTAGAC
AlwNI 2114 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
2848 CAGTTGCTG
AocI 1961 CCTCAGG CC^T[ACGT]AGG
3170 CCTCAGG
BsgI 704 CTGCAC GTGCAG(16/14)
941 GTGCAG
BsmBI 154 CGTCTC CGTCTC(1/5)
1503 CGTCTC
BsmI 375 GAATGC GAATGC(1/-1)
2990 GAATGC
Bsp1407I 1023 TGTACA T^GTACA
2719 TGTACA
BsrDI 2210 GCAATG GCAATG(2/0)
2994 GCAATG
BsrGI 1023 TGTACA T^GTACA
2719 TGTACA
BstXI 2798 CCAAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3198 CCAAAGTGCTGG
Bsu36I 1961 CCTCAGG CC^T[ACGT]AGG
3170 CCTCAGG
DraI 549 TTTAAA TTT^AAA
1311 TTTAAA
Eco81I 1961 CCTCAGG CC^T[ACGT]AGG
3170 CCTCAGG
EcoT22I 422 ATGCAT ATGCA^T
745 ATGCAT
HaeII 132 GGCGCT [GA]GCGC^[CT]
187 GGCGCC
MfeI 1027 CAATTG C^AATTG
2933 CAATTG
MunI 1027 CAATTG C^AATTG
2933 CAATTG
NdeI 671 CATATG CA^TATG
2827 CATATG
NsiI 422 ATGCAT ATGCA^T
745 ATGCAT
NspI 1041 GCATGC [GA]CATG^[CT]
2536 GCATGC
PvuII 1333 CAGCTG CAG^CTG
2255 CAGCTG
SacI 2430 GAGCTC GAGCT^C
2765 GAGCTC
SphI 1041 GCATGC GCATG^C
2536 GCATGC
SspBI 1023 TGTACA T^GTACA
2719 TGTACA
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BalI BanI
BanII BbvCI BbvI BfaI BglII
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MvaI MwoI NcoI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII