(Circular) (Six-base) MAPSORT of: pFN21AB7314.seq Check: 1112 from: 1 to: 7942 pFN21AB7314 7942 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5100 5100 Size: 7942 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5752 278 Size: 53 83 186 936 4216 2468 Fragments arranged by size: 4216 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2412 4449 5285 1937 Size: 475 2037 836 4594 Fragments arranged by size: 4594 2037 836 475 Acc65I G'GTAC_C Cuts at: 5263 5263 Size: 7942 AclI AA'CG_TT Cuts at: 1563 3142 6069 6442 1563 Size: 1579 2927 373 3063 Fragments arranged by size: 3063 2927 1579 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2277 4290 6005 7389 7657 1632 Size: 645 2013 1715 1384 268 1917 Fragments arranged by size: 2013 1917 1715 1384 645 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7745 AflIII A'CryG_T Cuts at: 1203 6841 7818 1203 Size: 5638 977 1327 Fragments arranged by size: 5638 1327 977 AgeI A'CCGG_T Cuts at: 6773 6773 Size: 7942 AhdI GACnn_n'nnGTC Cuts at: 2545 6671 2545 Size: 4126 3816 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7942 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7942 AlwNI CAG_nnn'CTG Cuts at: 4124 4858 5685 7257 4124 Size: 734 827 1572 4809 Fragments arranged by size: 4809 1572 827 734 ApaLI G'TGCA_C Cuts at: 5999 7155 5999 Size: 1156 6786 ApoI r'AATT_y Cuts at: 1493 2499 2613 3179 3417 3600 4156 5251 5477 Size: 1006 114 566 238 183 556 1095 226 Cuts at: 5477 5629 7738 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1095 1006 566 556 238 226 183 152 114 AseI AT'TA_AT Cuts at: 160 1032 4163 6496 160 Size: 872 3131 2333 1606 Fragments arranged by size: 3131 2333 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7942 AvaI C'yCGr_G Cuts at: 1870 1943 5238 5267 1870 Size: 73 3295 29 4545 Fragments arranged by size: 4545 3295 73 29 AvrII C'CTAG_G Cuts at: 3067 3067 Size: 7942 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3483 3516 3483 Size: 33 7909 BamHI G'GATC_C Cuts at: 4882 5272 5639 4882 Size: 390 367 7185 Fragments arranged by size: 7185 390 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2192 4030 5088 5263 6718 Size: 325 217 627 405 1838 1058 175 1455 Cuts at: 6718 618 Size: 1842 Fragments arranged by size: 1842 1838 1455 1058 627 405 325 217 175 BanII G_rGCy'C Cuts at: 1391 2520 4439 4774 5261 1391 Size: 1129 1919 335 487 4072 Fragments arranged by size: 4072 1919 1129 487 335 BbeI G_GCGC'C Cuts at: 2196 2196 Size: 7942 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2210 928 Size: 971 311 6660 Fragments arranged by size: 6660 971 311 BbvCI CC'TCA_GC Cuts at: 4179 5060 5194 4179 Size: 881 134 6927 Fragments arranged by size: 6927 881 134 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6133 6167 1903 Size: 34 4196 34 3678 Fragments arranged by size: 4196 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5834 7044 5834 Size: 1210 6732 BclI T'GATC_A Cuts at: 1548 4279 1548 Size: 2731 5211 BfrBI ATG'CAT Cuts at: 2429 2752 2429 Size: 323 7619 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6553 136 Size: 107 122 71 631 605 4881 1525 Fragments arranged by size: 4881 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4472 4485 4783 4925 7937 4472 Size: 13 298 142 3012 4477 Fragments arranged by size: 4477 3012 298 142 13 BlpI GC'TnA_GC Cuts at: 5359 5359 Size: 7942 Bme1580I G_kGCm'C Cuts at: 1790 6003 7159 1790 Size: 4213 1156 2573 Fragments arranged by size: 4213 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7942 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3844 3849 4512 Size: 416 53 83 399 24 2427 5 663 Cuts at: 4512 4888 5673 6631 442 Size: 376 785 958 1753 Fragments arranged by size: 2427 1753 958 785 663 416 399 376 83 53 24 5 BmtI G_CTAG'C Cuts at: 1056 4467 1056 Size: 3411 4531 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3540 4074 4788 6602 1239 Size: 2301 534 714 1814 2579 Fragments arranged by size: 2579 2301 1814 714 534 Bpu10I CC'TnA_GC Cuts at: 3399 3771 4179 5060 5194 3399 Size: 372 408 881 134 6147 Fragments arranged by size: 6147 881 408 372 134 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3084 6067 6932 7230 3084 Size: 2983 865 298 3796 Fragments arranged by size: 3796 2983 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6605 882 Size: 617 5106 2219 Fragments arranged by size: 5106 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4757 5733 493 Size: 711 3553 976 2702 Fragments arranged by size: 3553 2702 976 711 BsaBI GATnn'nnATC Cuts at: 7936 7936 Size: 7942 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2193 5749 6131 6766 Size: 53 83 186 936 660 3556 382 635 Cuts at: 6766 275 Size: 1451 Fragments arranged by size: 3556 1451 936 660 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6375 6773 7047 7194 7605 6375 Size: 398 274 147 411 6712 Fragments arranged by size: 6712 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4315 4345 1178 Size: 30 165 30 2912 30 4775 Fragments arranged by size: 4775 2912 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 1186 Size: 931 7011 BseYI C'CCAG_C Cuts at: 2477 4258 4420 5210 7145 7641 2477 Size: 1781 162 790 1935 496 2778 Fragments arranged by size: 2778 1935 1781 790 496 162 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2694 2967 1163 Size: 573 958 273 6138 Fragments arranged by size: 6138 958 573 273 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5392 6153 7181 1540 Size: 275 186 3391 761 1028 2301 Fragments arranged by size: 3391 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3490 4439 4774 6003 6088 7159 3490 Size: 949 335 1229 85 1071 4273 Fragments arranged by size: 4273 1229 1071 949 335 85 BsmI GAATG_Cn' Cuts at: 2386 5001 5453 5546 2386 Size: 2615 452 93 4782 Fragments arranged by size: 4782 2615 452 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2165 3514 2165 Size: 1349 6593 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2520 3490 4439 4774 5261 6003 6088 Size: 399 730 970 949 335 487 742 85 Cuts at: 6088 7159 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 970 949 742 730 487 399 335 85 BspEI T'CCGG_A Cuts at: 7605 7605 Size: 7942 BspHI T'CATG_A Cuts at: 2182 5831 7561 2182 Size: 3649 1730 2563 Fragments arranged by size: 3649 2563 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3687 4461 5100 5297 844 Size: 2843 774 639 197 3489 Fragments arranged by size: 3489 2843 774 639 197 BsrBI CCG'CTC Cuts at: 1394 1823 3770 5829 1394 Size: 429 1947 2059 3507 Fragments arranged by size: 3507 2059 1947 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4222 5006 6437 6611 65 Size: 1183 2974 784 1431 174 1396 Fragments arranged by size: 2974 1431 1396 1183 784 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6586 6773 1700 Size: 110 4776 187 2869 Fragments arranged by size: 4776 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3028 4724 96 Size: 2932 1696 3314 Fragments arranged by size: 3314 2932 1696 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7942 BssSI C'ACGA_G Cuts at: 6002 7014 6002 Size: 1012 6930 BstAPI GCAn_nnn'nTGC Cuts at: 794 2336 794 Size: 1542 6400 BstBI TT'CG_AA Cuts at: 6746 6746 Size: 7942 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7942 BstXI CCAn_nnnn'nTGG Cuts at: 4810 5210 4810 Size: 400 7542 BstYI r'GATC_y Cuts at: 1972 2764 2833 4022 4472 4485 4783 4882 4925 Size: 792 69 1189 450 13 298 99 43 Cuts at: 4925 5272 5639 6024 6041 7493 7579 7591 7937 Size: 347 367 385 17 1452 86 12 346 Cuts at: 7937 1972 Size: 1977 Fragments arranged by size: 1977 1452 1189 792 450 385 367 347 346 298 99 86 69 43 17 13 12 Bsu36I CC'TnA_GG Cuts at: 3967 5176 3967 Size: 1209 6733 BtgI C'CryG_G Cuts at: 513 1065 3040 3743 4141 513 Size: 552 1975 703 398 4314 Fragments arranged by size: 4314 1975 703 552 398 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7203 Fragments arranged by size: 7203 683 56 BtsI GCAGTG_nn' Cuts at: 2025 3446 5018 5458 6252 6272 2025 Size: 1421 1572 440 794 20 3695 Fragments arranged by size: 3695 1572 1421 794 440 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7942 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7907 DraI TTT'AAA Cuts at: 2556 3318 5246 2556 Size: 762 1928 5252 Fragments arranged by size: 5252 1928 762 DraIII CAC_nnn'GTG Cuts at: 3485 3485 Size: 7942 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6949 809 Size: 507 5633 1802 Fragments arranged by size: 5633 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2845 3214 5149 Size: 54 1425 50 275 18 1015 369 1935 Cuts at: 5149 5389 6278 7907 8 Size: 240 889 1629 43 Fragments arranged by size: 1935 1629 1425 1015 889 369 275 240 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5389 1537 Size: 275 3577 4090 Fragments arranged by size: 4090 3577 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2094 2168 3732 4300 4309 5872 1353 Size: 741 74 1564 568 9 1563 3423 Fragments arranged by size: 3423 1564 1563 741 568 74 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2087 5019 6529 6903 7049 387 Size: 1064 138 498 2932 1510 374 146 1280 Fragments arranged by size: 2932 1510 1280 1064 498 374 146 138 EcoICRI GAG'CTC Cuts at: 4437 4772 4437 Size: 335 7607 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2277 3540 4074 4290 4788 6005 6602 Size: 393 645 1263 534 216 498 1217 597 Cuts at: 6602 7389 7657 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1263 1217 787 645 597 534 498 393 268 216 EcoO109I rG'GnC_Cy Cuts at: 3809 3809 Size: 7942 EcoRI G'AATT_C Cuts at: 3417 5251 5629 3417 Size: 1834 378 5730 Fragments arranged by size: 5730 1834 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2480 2480 Size: 7942 FspI TGC'GCA Cuts at: 2017 6448 2017 Size: 4431 3511 HaeII r_GCGC'y Cuts at: 1678 2141 2196 7089 1678 Size: 463 55 4893 2531 Fragments arranged by size: 4893 2531 463 55 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2502 2531 2534 2563 2641 Size: 32 135 32 930 29 3 29 78 Cuts at: 2641 2673 5000 5032 6583 6615 6657 6689 1373 Size: 32 2327 32 1551 32 42 32 2626 Fragments arranged by size: 2626 2327 1551 930 135 78 42 32 32 32 32 32 32 29 29 3 HincII GTy'rAC Cuts at: 669 1657 1938 2473 5286 5532 669 Size: 988 281 535 2813 246 3079 Fragments arranged by size: 3079 2813 988 535 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7942 HpaI GTT'AAC Cuts at: 5532 5532 Size: 7942 KasI G'GCGC_C Cuts at: 2192 2192 Size: 7942 KpnI G_GTAC'C Cuts at: 5267 5267 Size: 7942 MfeI C'AATT_G Cuts at: 3032 4938 5541 3032 Size: 1906 603 5433 Fragments arranged by size: 5433 1906 603 MluI A'CGCG_T Cuts at: 7818 7818 Size: 7942 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2292 2726 7056 7240 1305 Size: 987 434 4330 184 2007 Fragments arranged by size: 4330 2007 987 434 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2847 3216 5151 10 Size: 54 1425 343 1015 369 1935 2801 Fragments arranged by size: 2801 1935 1425 1015 369 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2187 2637 3045 5900 6259 6418 518 Size: 648 1021 450 408 2855 359 159 2042 Fragments arranged by size: 2855 2042 1021 648 450 408 359 159 MspA1I CmG'CkG Cuts at: 1606 2039 3340 4262 5358 6035 7183 7428 1606 Size: 433 1301 922 1096 677 1148 245 2120 Fragments arranged by size: 2120 1301 1148 1096 922 677 433 245 NarI GG'CG_CC Cuts at: 2193 2193 Size: 7942 NcoI C'CATG_G Cuts at: 513 1065 3040 3743 4141 513 Size: 552 1975 703 398 4314 Fragments arranged by size: 4314 1975 703 552 398 NdeI CA'TA_TG Cuts at: 387 2677 4833 387 Size: 2290 2156 3496 Fragments arranged by size: 3496 2290 2156 NheI G'CTAG_C Cuts at: 1052 4463 1052 Size: 3411 4531 NotI GC'GGCC_GC Cuts at: 5389 5389 Size: 7942 NsiI A_TGCA'T Cuts at: 2431 2754 2431 Size: 323 7619 NspI r_CATG'y Cuts at: 1132 1229 3050 4545 5300 6845 1132 Size: 97 1821 1495 755 1545 2229 Fragments arranged by size: 2229 1821 1545 1495 755 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7942 PciI A'CATG_T Cuts at: 6841 6841 Size: 7942 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7942 PfoI T'CCnGG_A Cuts at: 4974 4974 Size: 7942 PmeI GTTT'AAAC Cuts at: 5246 5246 Size: 7942 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6011 6043 7524 7556 6011 Size: 32 1481 32 6397 Fragments arranged by size: 6397 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3809 3809 Size: 7942 PshAI GACnn'nnGTC Cuts at: 1613 2157 1613 Size: 544 7398 PsiI TTA'TAA Cuts at: 5512 5512 Size: 7942 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2525 2557 2525 Size: 32 7910 PstI C_TGCA'G Cuts at: 830 3574 3684 5025 5294 830 Size: 2744 110 1341 269 3478 Fragments arranged by size: 3478 2744 1341 269 110 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7942 PvuII CAG'CTG Cuts at: 3340 4262 3340 Size: 922 7020 SacI G_AGCT'C Cuts at: 4439 4774 4439 Size: 335 7607 SalI G'TCGA_C Cuts at: 1936 5284 1936 Size: 3348 4594 SapI GCTCTTCn'nnn_ Cuts at: 3732 3732 Size: 7942 SbfI CC_TGCA'GG Cuts at: 5294 5294 Size: 7942 ScaI AGT'ACT Cuts at: 6190 7902 6190 Size: 1712 6230 SfcI C'TryA_G Cuts at: 826 1045 3185 3570 3680 5021 5290 6425 7106 Size: 219 2140 385 110 1341 269 1135 681 Cuts at: 7106 7297 826 Size: 191 1471 Fragments arranged by size: 2140 1471 1341 1135 681 385 269 219 191 110 SfoI GGC'GCC Cuts at: 2194 2194 Size: 7942 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7942 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7942 SmaI CCC'GGG Cuts at: 1872 5240 5269 1872 Size: 3368 29 4545 Fragments arranged by size: 4545 3368 29 SmlI C'TyrA_G Cuts at: 820 1017 1943 3099 6046 6947 7209 820 Size: 197 926 1156 2947 901 262 1553 Fragments arranged by size: 2947 1553 1156 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5733 493 Size: 5240 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7942 SphI G_CATG'C Cuts at: 1132 3050 4545 5300 1132 Size: 1918 1495 755 3774 Fragments arranged by size: 3774 1918 1495 755 SspI AAT'ATT Cuts at: 5 52 4252 5866 5 Size: 47 4200 1614 2081 Fragments arranged by size: 4200 2081 1614 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7942 StyI C'CwwG_G Cuts at: 513 1065 2401 2493 3040 3067 3743 4026 4141 Size: 552 1336 92 547 27 676 283 115 Cuts at: 4141 5381 513 Size: 1240 3074 Fragments arranged by size: 3074 1336 1240 676 552 547 283 115 92 27 SwaI ATTT'AAAT Cuts at: 3318 3318 Size: 7942 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4272 4675 5969 5986 6139 6324 6769 Size: 1255 2692 403 1294 17 153 185 445 Cuts at: 6769 325 Size: 1498 Fragments arranged by size: 2692 1498 1294 1255 445 403 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2594 3028 3238 Size: 274 80 33 51 1443 617 434 210 Cuts at: 3238 3611 4724 6188 7900 96 Size: 373 1113 1464 1712 138 Fragments arranged by size: 1712 1464 1443 1113 617 434 373 274 210 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5206 5238 7902 7934 5206 Size: 32 2664 32 5214 Fragments arranged by size: 5214 2664 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7942 XbaI T'CTAG_A Cuts at: 5278 5278 Size: 7942 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4216 4216 Size: 7942 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7942 XmaI C'CCGG_G Cuts at: 1870 5238 5267 1870 Size: 3368 29 4545 Fragments arranged by size: 4545 3368 29 XmnI GAAnn'nnTTC Cuts at: 2703 6071 2703 Size: 3368 4574 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5750 276 Size: 53 83 186 936 4216 2468 Fragments arranged by size: 4216 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BplI BsiWI BstZ17I EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SrfI