Restriction map for
pFN21AB7314
(Linear) (Six-base) MAPSORT of: KIBB7314.seq Check: 8118 from: 1 to: 3237
KIBB7314.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3095 3237
Size: 3095 142
AccI GT'mk_AC
Cuts at: 0 407 2444 3237
Size: 407 2037 793
Fragments arranged by size:
2037 793 407
AclI AA'CG_TT
Cuts at: 0 1137 3237
Size: 1137 2100
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 2285 3237
Size: 272 2013 952
Fragments arranged by size:
2013 952 272
AhdI GACnn_n'nnGTC
Cuts at: 0 540 3237
Size: 540 2697
AlwNI CAG_nnn'CTG
Cuts at: 0 2119 2853 3237
Size: 2119 734 384
Fragments arranged by size:
2119 734 384
ApoI r'AATT_y
Cuts at: 0 494 608 1174 1412 1595 2151 3237
Size: 494 114 566 238 183 556 1086
Fragments arranged by size:
1086 566 556 494 238 183 114
AseI AT'TA_AT
Cuts at: 0 2158 3237
Size: 2158 1079
AvrII C'CTAG_G
Cuts at: 0 1062 3237
Size: 1062 2175
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1478 1511 3237
Size: 1478 33 1726
Fragments arranged by size:
1726 1478 33
BamHI G'GATC_C
Cuts at: 0 2877 3237
Size: 2877 360
BanI G'GyrC_C
Cuts at: 0 187 2025 3083 3237
Size: 187 1838 1058 154
Fragments arranged by size:
1838 1058 187 154
BanII G_rGCy'C
Cuts at: 0 515 2434 2769 3237
Size: 515 1919 335 468
Fragments arranged by size:
1919 515 468 335
BbeI G_GCGC'C
Cuts at: 0 191 3237
Size: 191 3046
BbsI GAAGACnn'nnnn_
Cuts at: 0 205 3237
Size: 205 3032
BbvCI CC'TCA_GC
Cuts at: 0 2174 3055 3189 3237
Size: 2174 881 134 48
Fragments arranged by size:
2174 881 134 48
BclI T'GATC_A
Cuts at: 0 2274 3237
Size: 2274 963
BfrBI ATG'CAT
Cuts at: 0 424 747 3237
Size: 424 323 2490
Fragments arranged by size:
2490 424 323
BglII A'GATC_T
Cuts at: 0 2467 2480 2778 2920 3237
Size: 2467 13 298 142 317
Fragments arranged by size:
2467 317 298 142 13
BmrI ACTGGGnnnn_n'
Cuts at: 0 1839 1844 2507 2883 3237
Size: 1839 5 663 376 354
Fragments arranged by size:
1839 663 376 354 5
BmtI G_CTAG'C
Cuts at: 0 2462 3237
Size: 2462 775
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1535 2069 2783 3237
Size: 1535 534 714 454
Fragments arranged by size:
1535 714 534 454
Bpu10I CC'TnA_GC
Cuts at: 0 1394 1766 2174 3055 3189 3237
Size: 1394 372 408 881 134 48
Fragments arranged by size:
1394 881 408 372 134 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1079 3237
Size: 1079 2158
BsaAI yAC'GTr
Cuts at: 0 2752 3237
Size: 2752 485
BsaHI Gr'CG_yC
Cuts at: 0 188 3237
Size: 188 3049
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2310 2340 3237
Size: 2310 30 897
Fragments arranged by size:
2310 897 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 3237
Size: 112 3125
BseYI C'CCAG_C
Cuts at: 0 472 2253 2415 3205 3237
Size: 472 1781 162 790 32
Fragments arranged by size:
1781 790 472 162 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 689 962 3237
Size: 689 273 2275
Fragments arranged by size:
2275 689 273
BsiHKAI G_wGCw'C
Cuts at: 0 1485 2434 2769 3237
Size: 1485 949 335 468
Fragments arranged by size:
1485 949 468 335
BsmI GAATG_Cn'
Cuts at: 0 381 2996 3237
Size: 381 2615 241
Fragments arranged by size:
2615 381 241
BsmBI CGTCTCn'nnnn_
Cuts at: 0 160 1509 3237
Size: 160 1349 1728
Fragments arranged by size:
1728 1349 160
Bsp1286I G_dGCh'C
Cuts at: 0 515 1485 2434 2769 3237
Size: 515 970 949 335 468
Fragments arranged by size:
970 949 515 468 335
BspHI T'CATG_A
Cuts at: 0 177 3237
Size: 177 3060
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1682 2456 3095 3237
Size: 1682 774 639 142
Fragments arranged by size:
1682 774 639 142
BsrBI CCG'CTC
Cuts at: 0 1765 3237
Size: 1765 1472
BsrDI GCAATG_nn'
Cuts at: 0 2217 3001 3237
Size: 2217 784 236
Fragments arranged by size:
2217 784 236
BsrGI T'GTAC_A
Cuts at: 0 1023 2719 3237
Size: 1023 1696 518
Fragments arranged by size:
1696 1023 518
BstAPI GCAn_nnn'nTGC
Cuts at: 0 331 3237
Size: 331 2906
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2805 3205 3237
Size: 2805 400 32
Fragments arranged by size:
2805 400 32
BstYI r'GATC_y
Cuts at: 0 759 828 2017 2467 2480 2778 2877 2920
Size: 759 69 1189 450 13 298 99 43
Cuts at: 2920 3237
Size: 317
Fragments arranged by size:
1189 759 450 317 298 99 69 43
13
Bsu36I CC'TnA_GG
Cuts at: 0 1962 3171 3237
Size: 1962 1209 66
Fragments arranged by size:
1962 1209 66
BtgI C'CryG_G
Cuts at: 0 1035 1738 2136 3237
Size: 1035 703 398 1101
Fragments arranged by size:
1101 1035 703 398
BtsI GCAGTG_nn'
Cuts at: 0 20 1441 3013 3237
Size: 20 1421 1572 224
Fragments arranged by size:
1572 1421 224 20
DraI TTT'AAA
Cuts at: 0 551 1313 3237
Size: 551 762 1924
Fragments arranged by size:
1924 762 551
DraIII CAC_nnn'GTG
Cuts at: 0 1480 3237
Size: 1480 1757
EaeI y'GGCC_r
Cuts at: 0 840 1209 3144 3237
Size: 840 369 1935 93
Fragments arranged by size:
1935 840 369 93
EarI CTCTTCn'nnn_
Cuts at: 0 89 163 1727 2295 2304 3237
Size: 89 74 1564 568 9 933
Fragments arranged by size:
1564 933 568 89 74 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 82 3014 3237
Size: 82 2932 223
Fragments arranged by size:
2932 223 82
EcoICRI GAG'CTC
Cuts at: 0 2432 2767 3237
Size: 2432 335 470
Fragments arranged by size:
2432 470 335
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 1535 2069 2285 2783 3237
Size: 272 1263 534 216 498 454
Fragments arranged by size:
1263 534 498 454 272 216
EcoO109I rG'GnC_Cy
Cuts at: 0 1804 3237
Size: 1804 1433
EcoRI G'AATT_C
Cuts at: 0 1412 3237
Size: 1412 1825
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 475 3237
Size: 475 2762
FspI TGC'GCA
Cuts at: 0 12 3237
Size: 12 3225
HaeII r_GCGC'y
Cuts at: 0 136 191 3237
Size: 136 55 3046
Fragments arranged by size:
3046 136 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 497 526 529 558 636 668 2995 3027
Size: 497 29 3 29 78 32 2327 32
Cuts at: 3027 3237
Size: 210
Fragments arranged by size:
2327 497 210 78 32 32 29 29
3
HincII GTy'rAC
Cuts at: 0 468 3237
Size: 468 2769
KasI G'GCGC_C
Cuts at: 0 187 3237
Size: 187 3050
MfeI C'AATT_G
Cuts at: 0 1027 2933 3237
Size: 1027 1906 304
Fragments arranged by size:
1906 1027 304
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 721 3237
Size: 287 434 2516
Fragments arranged by size:
2516 434 287
MscI TGG'CCA
Cuts at: 0 842 1211 3146 3237
Size: 842 369 1935 91
Fragments arranged by size:
1935 842 369 91
MslI CAynn'nnrTG
Cuts at: 0 182 632 1040 3237
Size: 182 450 408 2197
Fragments arranged by size:
2197 450 408 182
MspA1I CmG'CkG
Cuts at: 0 34 1335 2257 3237
Size: 34 1301 922 980
Fragments arranged by size:
1301 980 922 34
NarI GG'CG_CC
Cuts at: 0 188 3237
Size: 188 3049
NcoI C'CATG_G
Cuts at: 0 1035 1738 2136 3237
Size: 1035 703 398 1101
Fragments arranged by size:
1101 1035 703 398
NdeI CA'TA_TG
Cuts at: 0 672 2828 3237
Size: 672 2156 409
Fragments arranged by size:
2156 672 409
NheI G'CTAG_C
Cuts at: 0 2458 3237
Size: 2458 779
NsiI A_TGCA'T
Cuts at: 0 426 749 3237
Size: 426 323 2488
Fragments arranged by size:
2488 426 323
NspI r_CATG'y
Cuts at: 0 1045 2540 3237
Size: 1045 1495 697
Fragments arranged by size:
1495 1045 697
PfoI T'CCnGG_A
Cuts at: 0 2969 3237
Size: 2969 268
PpuMI rG'GwC_Cy
Cuts at: 0 1804 3237
Size: 1804 1433
PshAI GACnn'nnGTC
Cuts at: 0 152 3237
Size: 152 3085
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 520 552 3237
Size: 520 32 2685
Fragments arranged by size:
2685 520 32
PstI C_TGCA'G
Cuts at: 0 1569 1679 3020 3237
Size: 1569 110 1341 217
Fragments arranged by size:
1569 1341 217 110
PvuII CAG'CTG
Cuts at: 0 1335 2257 3237
Size: 1335 922 980
Fragments arranged by size:
1335 980 922
SacI G_AGCT'C
Cuts at: 0 2434 2769 3237
Size: 2434 335 468
Fragments arranged by size:
2434 468 335
SapI GCTCTTCn'nnn_
Cuts at: 0 1727 3237
Size: 1727 1510
SfcI C'TryA_G
Cuts at: 0 1180 1565 1675 3016 3237
Size: 1180 385 110 1341 221
Fragments arranged by size:
1341 1180 385 221 110
SfoI GGC'GCC
Cuts at: 0 189 3237
Size: 189 3048
SmlI C'TyrA_G
Cuts at: 0 1094 3237
Size: 1094 2143
SphI G_CATG'C
Cuts at: 0 1045 2540 3237
Size: 1045 1495 697
Fragments arranged by size:
1495 1045 697
SspI AAT'ATT
Cuts at: 0 2247 3237
Size: 2247 990
StyI C'CwwG_G
Cuts at: 0 396 488 1035 1062 1738 2021 2136 3237
Size: 396 92 547 27 676 283 115 1101
Fragments arranged by size:
1101 676 547 396 283 115 92 27
SwaI ATTT'AAAT
Cuts at: 0 1313 3237
Size: 1313 1924
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2267 2670 3237
Size: 2267 403 567
Fragments arranged by size:
2267 567 403
TatI w'GTAC_w
Cuts at: 0 589 1023 1233 1606 2719 3237
Size: 589 434 210 373 1113 518
Fragments arranged by size:
1113 589 518 434 373 210
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3201 3233 3237
Size: 3201 32 4
Fragments arranged by size:
3201 32 4
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2211 3237
Size: 2211 1026
XmnI GAAnn'nnTTC
Cuts at: 0 698 3237
Size: 698 2539
Enzymes that do cut:
AarI AccI AclI AcuI AhdI AlwNI ApoI AseI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BclI BfrBI BglII BmrI BmtI BpmI Bpu10I BpuEI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI BstAPI
BstXI BstYI Bsu36I BtgI BtsI DraI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI MfeI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NsiI NspI PfoI
PpuMI PshAI PsrI PstI PvuII SacI SapI SfcI
SfoI SmlI SphI SspI StyI SwaI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AflIII AgeI AleI AlfI
AloI ApaI ApaLI AscI AsiSI AvaI BcgI BciVI
BglI BlpI Bme1580I BmgBI BplI BsaI BsaBI BsaWI
BsiEI BsiWI BspEI BsrFI BssHII BssSI BstBI BstEII
BstZ17I BtgZI ClaI CspCI DrdI EagI EcoNI EcoRV
FseI FspAI HindIII HpaI KpnI MluI NaeI NgoMIV
NotI NruI PacI PasI PciI PflMI PmeI PmlI
PpiI PsiI PspOMI PspXI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SrfI StuI Tth111I XbaI XhoI XmaI
ZraI