################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BcgI BciVI BfrI BlnI BlpI Bpu1102I BsaBI BsaI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PmeI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tsp509I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AflIII 1064 ACGTGT A^C[GA][CT]GT AgeI 704 ACCGGT A^CCGGT AlwI 987 GGATC GGATC(4/5) AvaI 19 CCCGAG C^[CT]CG[GA]G BbrPI 1063 CACGTG CAC^GTG BbsI 238 GTCTTC GAAGAC(2/6) BbvCI 151 GCTGAGG CCTCAGC(-5/-2) BclI 533 TGATCA T^GATCA BfaI 763 CTAG C^TAG BglI 40 GCCGAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 896 AGATCT A^GATCT BpuAI 238 GTCTTC GAAGAC(2/6) BsaWI 704 ACCGGT [AT]^CCGG[AT] BseRI 388 GAGGAG GAGGAG(10/8) BsmI 219 GCATTC GAATGC(1/-1) BsoBI 19 CCCGAG C^[CT]CG[GA]G BspHI 557 TCATGA T^CATGA BsrBI 141 GAGCGG CCGCTC(-3/-3) BsrFI 704 ACCGGT [GA]^CCGG[CT] BstXI 189 CCATGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 704 ACCGGT [GA]^CCGG[CT] DraIII 736 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 999 CTCTTC CTCTTC(1/4) FbaI 533 TGATCA T^GATCA HincII 1405 GTTAAC GT[CT]^[GA]AC HindII 1405 GTTAAC GT[CT]^[GA]AC HindIII 401 AAGCTT A^AGCTT HpaI 1405 GTTAAC GTT^AAC Ksp632I 999 CTCTTC CTCTTC(1/4) MaeI 763 CTAG C^TAG MseI 1406 TTAA T^TAA PinAI 704 ACCGGT A^CCGGT PmaCI 1063 CACGTG CAC^GTG PmlI 1063 CACGTG CAC^GTG PpuMI 1417 GGGACCT [GA]G^G[AT]CC[CT] PshAI 701 GACACCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 557 TCATGA T^CATGA SapI 998 GCTCTTC GCTCTTC(1/4) SexAI 737 ACCTGGT A^CC[AT]GGT SphI 276 GCATGC GCATG^C TfiI 1103 GATTC G^A[AT]TC Tru9I 1406 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AatII 922 GACGTC GACGT^C 1516 GACGTC AccII 753 CGCG CG^CG 804 CGCG AfaI 1347 GTAC GT^AC 1536 GTAC AspHI 817 GTGCTC G[AT]GC[AT]^C 1117 GAGCAC BalI 302 TGGCCA TGG^CCA 1431 TGGCCA BanI 623 GGCACC G^G[CT][GA]CC 758 GGCACC BpmI 1317 CTCCAG CTGGAG(16/14) 1288 CTGGAG BsaAI 1063 CACGTG [CT]AC^GT[GA] 1269 CACGTA BsiHKAI 817 GTGCTC G[AT]GC[AT]^C 1117 GAGCAC BsmAI 949 GTCTC GTCTC(1/5) 1367 GTCTC BspMI 110 ACCTGC ACCTGC(4/8) 928 ACCTGC BssSI 271 CACGAG CACGAG(-5/-1) 790 CACGAG BstAPI 105 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1148 GCAGCGGTTGC BstF5I 717 CATCC GGATG(2/0) 1334 GGATG BstUI 753 CGCG CG^CG 804 CGCG BstYI 896 AGATCT [GA]^GATC[CT] 987 GGATCT DraII 575 AGGCCCT [GA]G^G[ACGT]CC[CT] 1417 GGGACCT EcoO109I 575 AGGCCCT [GA]G^G[ACGT]CC[CT] 1417 GGGACCT FokI 717 CATCC GGATG(9/13) 1334 GGATG HaeII 54 AGCGCC [GA]GCGC^[CT] 1132 AGCGCC MaeIII 925 GTCAC ^GT[ACGT]AC 1481 GTGAC MflI 896 AGATCT [GA]^GATC[CT] 987 GGATCT MluNI 302 TGGCCA TGG^CCA 1431 TGGCCA MscI 302 TGGCCA TGG^CCA 1431 TGGCCA MspA1I 627 CCGCTG C[AC]G^C[GT]G 1149 CAGCGG MvnI 753 CGCG CG^CG 804 CGCG NcoI 32 CCATGG C^CATGG 942 CCATGG NspI 276 GCATGC [GA]CATG^[CT] 550 ACATGC PleI 947 GAGTC GAGTC(4/5) 1171 GAGTC RsaI 1347 GTAC GT^AC 1536 GTAC TaqI 505 TCGA T^CGA 962 TCGA Tsp45I 925 GTCAC ^GT[GC]AC 1481 GTGAC TthHB8I 505 TCGA T^CGA 962 TCGA XcmI 647 CCAGCTCCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 933 CCAAGTCTGCCATGG XhoII 896 AGATCT [GA]^GATC[CT] 987 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AcyI AluI AlwNI AvaII BanII BbvI BcnI BmyI BsaHI BsaJI BsgI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I FauI Fnu4HI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MboI MboII MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI StyI TseI TspRI