Restriction map for
pFN21AA1063
(Circular) (Six-base) MAPSORT of: pFN21AA1063.seq Check: 6457 from: 1 to: 6280
pFN21AA1063 6280 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2942 2942
Size: 6280
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2931 3525 4090 278
Size: 53 83 186 936 1395 594 565 2468
Fragments arranged by size:
2468 1395 936 594 565 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3623 1937
Size: 1686 4594
Acc65I G'GTAC_C
Cuts at: 3601 3601
Size: 6280
AclI AA'CG_TT
Cuts at: 1563 4407 4780 1563
Size: 2844 373 3063
Fragments arranged by size:
3063 2844 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2498 4343 5727 5995 1632
Size: 866 1845 1384 268 1917
Fragments arranged by size:
1917 1845 1384 866 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6083
AflIII A'CryG_T
Cuts at: 1203 3069 5179 6156 1203
Size: 1866 2110 977 1327
Fragments arranged by size:
2110 1866 1327 977
AgeI A'CCGG_T
Cuts at: 2709 5111 2709
Size: 2402 3878
AhdI GACnn_n'nnGTC
Cuts at: 5009 5009
Size: 6280
AleI CACnn'nnGTG
Cuts at: 1166 2634 3278 1166
Size: 1468 644 4168
Fragments arranged by size:
4168 1468 644
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6280
AlwNI CAG_nnn'CTG
Cuts at: 2116 2584 2658 3568 4023 5595 2116
Size: 468 74 910 455 1572 2801
Fragments arranged by size:
2801 1572 910 468 455 74
ApaLI G'TGCA_C
Cuts at: 4337 5493 4337
Size: 1156 5124
ApoI r'AATT_y
Cuts at: 1493 3589 3815 3967 6076 1493
Size: 2096 226 152 2109 1697
Fragments arranged by size:
2109 2096 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4834 160
Size: 872 3802 1606
Fragments arranged by size:
3802 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6280
AvaI C'yCGr_G
Cuts at: 1870 1943 2024 3605 1870
Size: 73 81 1581 4545
Fragments arranged by size:
4545 1581 81 73
BamHI G'GATC_C
Cuts at: 3610 3977 3610
Size: 367 5913
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2628 2763 3601 5056 618
Size: 325 217 627 841 135 838 1455 1842
Fragments arranged by size:
1842 1455 841 838 627 325 217 135
BanII G_rGCy'C
Cuts at: 1391 2025 2651 3032 3599 1391
Size: 634 626 381 567 4072
Fragments arranged by size:
4072 634 626 567 381
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2236 928
Size: 971 337 4972
Fragments arranged by size:
4972 971 337
BbvCI CC'TCA_GC
Cuts at: 2157 2157
Size: 6280
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4471 4505 1903
Size: 34 2534 34 3678
Fragments arranged by size:
3678 2534 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4172 5382 4172
Size: 1210 5070
BclI T'GATC_A
Cuts at: 1548 2538 1548
Size: 990 5290
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2051 4891 136
Size: 107 122 71 631 605 379 2840 1525
Fragments arranged by size:
2840 1525 631 605 379 122 107 71
BglII A'GATC_T
Cuts at: 2901 6275 2901
Size: 3374 2906
BlpI GC'TnA_GC
Cuts at: 3697 3697
Size: 6280
Bme1580I G_kGCm'C
Cuts at: 1790 4341 5497 1790
Size: 2551 1156 2573
Fragments arranged by size:
2573 2551 1156
BmgBI CAC'GTC
Cuts at: 1318 2821 1318
Size: 1503 4777
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4011 4969 442
Size: 416 53 83 399 24 2594 958 1753
Fragments arranged by size:
2594 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6280
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2695 2716 2695
Size: 21 6259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3307 3314 4940 1239
Size: 2068 7 1626 2579
Fragments arranged by size:
2579 2068 1626 7
Bpu10I CC'TnA_GC
Cuts at: 2157 3026 2157
Size: 869 5411
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4405 5270 5568 4405
Size: 865 298 5117
Fragments arranged by size:
5117 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4943 882
Size: 617 3444 2219
Fragments arranged by size:
3444 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3070 3276 4071 493
Size: 711 1866 206 795 2702
Fragments arranged by size:
2702 1866 795 711 206
BsaBI GATnn'nnATC
Cuts at: 6274 6274
Size: 6280
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2043 2205 2790 2928
Size: 53 83 186 936 510 162 585 138
Cuts at: 2928 3522 4087 4469 5104 275
Size: 594 565 382 635 1451
Fragments arranged by size:
1451 936 635 594 585 565 510 382
186 162 138 83 53
BsaWI w'CCGG_w
Cuts at: 2709 4713 5111 5385 5532 5943 2709
Size: 2004 398 274 147 411 3046
Fragments arranged by size:
3046 2004 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6055
Fragments arranged by size:
6055 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2408 1186
Size: 1222 5058
BseYI C'CCAG_C
Cuts at: 2651 5483 5979 2651
Size: 2832 496 2952
Fragments arranged by size:
2952 2832 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2500 2597 2723 3172 1163
Size: 573 764 97 126 449 4271
Fragments arranged by size:
4271 764 573 449 126 97
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3730 4491 5519 1540
Size: 275 186 1729 761 1028 2301
Fragments arranged by size:
2301 1729 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2826 3126 4341 4426 5497 2826
Size: 300 1215 85 1071 3609
Fragments arranged by size:
3609 1215 1071 300 85
BsmI GAATG_Cn'
Cuts at: 2224 3791 3884 2224
Size: 1567 93 4620
Fragments arranged by size:
4620 1567 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2651 2826 3032 3126 3599 4341
Size: 399 235 626 175 206 94 473 742
Cuts at: 4341 4426 5497 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 626 473 399 235 206
175 94 85
BspEI T'CCGG_A
Cuts at: 5943 5943
Size: 6280
BspHI T'CATG_A
Cuts at: 2562 4169 5899 2562
Size: 1607 1730 2943
Fragments arranged by size:
2943 1730 1607
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2124 2942 3635 844
Size: 1280 818 693 3489
Fragments arranged by size:
3489 1280 818 693
BsrBI CCG'CTC
Cuts at: 1394 1823 2148 4167 1394
Size: 429 325 2019 3507
Fragments arranged by size:
3507 2019 429 325
BsrDI GCAATG_nn'
Cuts at: 65 1248 4775 4949 65
Size: 1183 3527 174 1396
Fragments arranged by size:
3527 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2709 4924 5111 1700
Size: 110 899 2215 187 2869
Fragments arranged by size:
2869 2215 899 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6280
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6280
BssSI C'ACGA_G
Cuts at: 2276 2795 4340 5352 2276
Size: 519 1545 1012 3204
Fragments arranged by size:
3204 1545 1012 519
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2116 3159 794
Size: 1322 1043 3915
Fragments arranged by size:
3915 1322 1043
BstBI TT'CG_AA
Cuts at: 5084 5084
Size: 6280
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6280
BstXI CCAn_nnnn'nTGG
Cuts at: 2201 2201
Size: 6280
BstYI r'GATC_y
Cuts at: 1972 2901 2992 3610 3977 4362 4379 5831 5917
Size: 929 91 618 367 385 17 1452 86
Cuts at: 5917 5929 6275 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 929 618 385 367 346 91
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2037 2947 3363 513
Size: 552 939 33 910 416 3430
Fragments arranged by size:
3430 939 910 552 416 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2791 487
Size: 683 56 1565 3976
Fragments arranged by size:
3976 1565 683 56
BtsI GCAGTG_nn'
Cuts at: 2833 2878 3796 4590 4610 2833
Size: 45 918 794 20 4503
Fragments arranged by size:
4503 918 794 45 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6280
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6245
DraI TTT'AAA
Cuts at: 3584 3584
Size: 6280
DraIII CAC_nnn'GTG
Cuts at: 2746 2746
Size: 6280
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5287 809
Size: 507 3971 1802
Fragments arranged by size:
3971 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2015 2307 3436
Size: 54 1425 50 275 18 185 292 1129
Cuts at: 3436 3727 4616 6245 8
Size: 291 889 1629 43
Fragments arranged by size:
1629 1425 1129 889 292 291 275 185
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3727 1537
Size: 275 1915 4090
Fragments arranged by size:
4090 1915 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3010 4210 1353
Size: 1657 1200 3423
Fragments arranged by size:
3423 1657 1200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3277 4867 5241 5387 387
Size: 1064 138 1688 1590 374 146 1280
Fragments arranged by size:
1688 1590 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2498 3307 3314 4343 4940 5727 5995
Size: 393 866 809 7 1029 597 787 268
Cuts at: 5995 1239
Size: 1524
Fragments arranged by size:
1524 1029 866 809 787 597 393 268
7
EcoO109I rG'GnC_Cy
Cuts at: 2581 3423 2581
Size: 842 5438
EcoRI G'AATT_C
Cuts at: 3589 3967 3589
Size: 378 5902
FspI TGC'GCA
Cuts at: 4786 4786
Size: 6280
HaeII r_GCGC'y
Cuts at: 1678 2063 3141 5427 1678
Size: 385 1078 2286 2531
Fragments arranged by size:
2531 2286 1078 385
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4921 4953 4995 5027 1373
Size: 32 135 32 3349 32 42 32 2626
Fragments arranged by size:
3349 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3412 3624 3870 669
Size: 988 281 1474 212 246 3079
Fragments arranged by size:
3079 1474 988 281 246 212
HindIII A'AGCT_T
Cuts at: 748 2406 748
Size: 1658 4622
HpaI GTT'AAC
Cuts at: 3412 3870 3412
Size: 458 5822
KpnI G_GTAC'C
Cuts at: 3605 3605
Size: 6280
MfeI C'AATT_G
Cuts at: 3879 3879
Size: 6280
MluI A'CGCG_T
Cuts at: 6156 6156
Size: 6280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2407 5394 5578 1305
Size: 1102 2987 184 2007
Fragments arranged by size:
2987 2007 1102 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2309 3438 10
Size: 54 1425 343 477 1129 2852
Fragments arranged by size:
2852 1425 1129 477 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2199 2280 2634 3278 4238 4597 4756
Size: 648 1033 81 354 644 960 359 159
Cuts at: 4756 518
Size: 2042
Fragments arranged by size:
2042 1033 960 648 644 359 354 159
81
MspA1I CmG'CkG
Cuts at: 1606 2634 3156 3696 4373 5521 5766 1606
Size: 1028 522 540 677 1148 245 2120
Fragments arranged by size:
2120 1148 1028 677 540 522 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2037 2947 513
Size: 552 939 33 910 3846
Fragments arranged by size:
3846 939 910 552 33
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6280
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6280
NotI GC'GGCC_GC
Cuts at: 3727 3727
Size: 6280
NspI r_CATG'y
Cuts at: 1132 1229 2285 2559 3638 5183 1132
Size: 97 1056 274 1079 1545 2229
Fragments arranged by size:
2229 1545 1079 1056 274 97
PasI CC'CwG_GG
Cuts at: 1348 3032 1348
Size: 1684 4596
PciI A'CATG_T
Cuts at: 5179 5179
Size: 6280
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6280
PfoI T'CCnGG_A
Cuts at: 3525 3567 3525
Size: 42 6238
PmeI GTTT'AAAC
Cuts at: 3584 3584
Size: 6280
PmlI CAC'GTG
Cuts at: 3070 3070
Size: 6280
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4349 4381 5862 5894 4349
Size: 32 1481 32 4735
Fragments arranged by size:
4735 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3423 3423
Size: 6280
PshAI GACnn'nnGTC
Cuts at: 1613 2710 1613
Size: 1097 5183
PsiI TTA'TAA
Cuts at: 3850 3850
Size: 6280
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3533 3565 3533
Size: 32 6248
PstI C_TGCA'G
Cuts at: 830 3632 830
Size: 2802 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6280
SalI G'TCGA_C
Cuts at: 1936 3622 1936
Size: 1686 4594
SapI GCTCTTCn'nnn_
Cuts at: 3010 3010
Size: 6280
SbfI CC_TGCA'GG
Cuts at: 3632 3632
Size: 6280
ScaI AGT'ACT
Cuts at: 4528 6240 4528
Size: 1712 4568
SexAI A'CCwGG_T
Cuts at: 2742 2742
Size: 6280
SfcI C'TryA_G
Cuts at: 826 1045 3628 4763 5444 5635 826
Size: 219 2583 1135 681 191 1471
Fragments arranged by size:
2583 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6280
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6280
SmaI CCC'GGG
Cuts at: 1872 3607 1872
Size: 1735 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4384 5285 5547 820
Size: 197 926 2441 901 262 1553
Fragments arranged by size:
2441 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4071 493
Size: 3578 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6280
SphI G_CATG'C
Cuts at: 1132 2285 3638 1132
Size: 1153 1353 3774
Fragments arranged by size:
3774 1353 1153
SspI AAT'ATT
Cuts at: 5 52 4204 5
Size: 47 4152 2081
Fragments arranged by size:
4152 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6280
StyI C'CwwG_G
Cuts at: 513 1065 2004 2037 2388 2545 2947 3426 3504
Size: 552 939 33 351 157 402 479 78
Cuts at: 3504 3719 513
Size: 215 3074
Fragments arranged by size:
3074 939 552 479 402 351 215 157
78 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4307 4324 4477 4662 5107 325
Size: 1255 2727 17 153 185 445 1498
Fragments arranged by size:
2727 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4526 6238 96
Size: 274 80 33 51 1443 2549 1712 138
Fragments arranged by size:
2549 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6240 6272 6240
Size: 32 6248
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6280
XbaI T'CTAG_A
Cuts at: 3616 3616
Size: 6280
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2659 2945 2659
Size: 286 5994
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6280
XmaI C'CCGG_G
Cuts at: 1870 3605 1870
Size: 1735 4545
XmnI GAAnn'nnTTC
Cuts at: 4409 4409
Size: 6280
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2929 3523 4088 276
Size: 53 83 186 936 1395 594 565 2468
Fragments arranged by size:
2468 1395 936 594 565 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspOMI PspXI PvuII RsrII SacI SacII SanDI
SfiI SfoI SrfI SwaI