Restriction map for
pFN21AA1063
(Linear) (Six-base) MAPSORT of: KIAA1063.seq Check: 5376 from: 1 to: 1575
KIAA1063.orf
With 182 enzymes: SgfI *
July 15, 2010 18:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 937 1575
Size: 937 638
AatII G_ACGT'C
Cuts at: 0 926 1520 1575
Size: 926 594 55
Fragments arranged by size:
926 594 55
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 1575
Size: 493 1082
AflIII A'CryG_T
Cuts at: 0 1064 1575
Size: 1064 511
AgeI A'CCGG_T
Cuts at: 0 704 1575
Size: 704 871
AleI CACnn'nnGTG
Cuts at: 0 629 1273 1575
Size: 629 644 302
Fragments arranged by size:
644 629 302
AlwNI CAG_nnn'CTG
Cuts at: 0 111 579 653 1563 1575
Size: 111 468 74 910 12
Fragments arranged by size:
910 468 111 74 12
AvaI C'yCGr_G
Cuts at: 0 19 1575
Size: 19 1556
BanI G'GyrC_C
Cuts at: 0 623 758 1575
Size: 623 135 817
Fragments arranged by size:
817 623 135
BanII G_rGCy'C
Cuts at: 0 20 646 1027 1575
Size: 20 626 381 548
Fragments arranged by size:
626 548 381 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 231 1575
Size: 231 1344
BbvCI CC'TCA_GC
Cuts at: 0 152 1575
Size: 152 1423
BclI T'GATC_A
Cuts at: 0 533 1575
Size: 533 1042
BglI GCCn_nnn'nGGC
Cuts at: 0 46 1575
Size: 46 1529
BglII A'GATC_T
Cuts at: 0 896 1575
Size: 896 679
BmgBI CAC'GTC
Cuts at: 0 816 1575
Size: 816 759
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 690 711 1575
Size: 690 21 864
Fragments arranged by size:
864 690 21
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1302 1309 1575
Size: 1302 7 266
Fragments arranged by size:
1302 266 7
Bpu10I CC'TnA_GC
Cuts at: 0 152 1021 1575
Size: 152 869 554
Fragments arranged by size:
869 554 152
BsaAI yAC'GTr
Cuts at: 0 1065 1271 1575
Size: 1065 206 304
Fragments arranged by size:
1065 304 206
BsaHI Gr'CG_yC
Cuts at: 0 38 200 785 923 1517 1575
Size: 38 162 585 138 594 58
Fragments arranged by size:
594 585 162 138 58 38
BsaWI w'CCGG_w
Cuts at: 0 704 1575
Size: 704 871
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 403 1575
Size: 403 1172
BseYI C'CCAG_C
Cuts at: 0 646 1575
Size: 646 929
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 495 592 718 1167 1575
Size: 495 97 126 449 408
Fragments arranged by size:
495 449 408 126 97
BsiHKAI G_wGCw'C
Cuts at: 0 821 1121 1575
Size: 821 300 454
Fragments arranged by size:
821 454 300
BsmI GAATG_Cn'
Cuts at: 0 219 1575
Size: 219 1356
Bsp1286I G_dGCh'C
Cuts at: 0 20 646 821 1027 1121 1575
Size: 20 626 175 206 94 454
Fragments arranged by size:
626 454 206 175 94 20
BspHI T'CATG_A
Cuts at: 0 557 1575
Size: 557 1018
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 119 937 1575
Size: 119 818 638
Fragments arranged by size:
818 638 119
BsrBI CCG'CTC
Cuts at: 0 143 1575
Size: 143 1432
BsrFI r'CCGG_y
Cuts at: 0 704 1575
Size: 704 871
BssSI C'ACGA_G
Cuts at: 0 271 790 1575
Size: 271 519 785
Fragments arranged by size:
785 519 271
BstAPI GCAn_nnn'nTGC
Cuts at: 0 111 1154 1575
Size: 111 1043 421
Fragments arranged by size:
1043 421 111
BstXI CCAn_nnnn'nTGG
Cuts at: 0 196 1575
Size: 196 1379
BstYI r'GATC_y
Cuts at: 0 896 987 1575
Size: 896 91 588
Fragments arranged by size:
896 588 91
BtgI C'CryG_G
Cuts at: 0 32 942 1358 1575
Size: 32 910 416 217
Fragments arranged by size:
910 416 217 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 786 1575
Size: 786 789
BtsI GCAGTG_nn'
Cuts at: 0 828 873 1575
Size: 828 45 702
Fragments arranged by size:
828 702 45
DraIII CAC_nnn'GTG
Cuts at: 0 741 1575
Size: 741 834
EaeI y'GGCC_r
Cuts at: 0 10 302 1431 1575
Size: 10 292 1129 144
Fragments arranged by size:
1129 292 144 10
EarI CTCTTCn'nnn_
Cuts at: 0 1005 1575
Size: 1005 570
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1272 1575
Size: 1272 303
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 1302 1309 1575
Size: 493 809 7 266
Fragments arranged by size:
809 493 266 7
EcoO109I rG'GnC_Cy
Cuts at: 0 576 1418 1575
Size: 576 842 157
Fragments arranged by size:
842 576 157
HaeII r_GCGC'y
Cuts at: 0 58 1136 1575
Size: 58 1078 439
Fragments arranged by size:
1078 439 58
HincII GTy'rAC
Cuts at: 0 1407 1575
Size: 1407 168
HindIII A'AGCT_T
Cuts at: 0 401 1575
Size: 401 1174
HpaI GTT'AAC
Cuts at: 0 1407 1575
Size: 1407 168
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 402 1575
Size: 402 1173
MscI TGG'CCA
Cuts at: 0 304 1433 1575
Size: 304 1129 142
Fragments arranged by size:
1129 304 142
MslI CAynn'nnrTG
Cuts at: 0 194 275 629 1273 1575
Size: 194 81 354 644 302
Fragments arranged by size:
644 354 302 194 81
MspA1I CmG'CkG
Cuts at: 0 629 1151 1575
Size: 629 522 424
Fragments arranged by size:
629 522 424
NcoI C'CATG_G
Cuts at: 0 32 942 1575
Size: 32 910 633
Fragments arranged by size:
910 633 32
NspI r_CATG'y
Cuts at: 0 280 554 1575
Size: 280 274 1021
Fragments arranged by size:
1021 280 274
PasI CC'CwG_GG
Cuts at: 0 1027 1575
Size: 1027 548
PfoI T'CCnGG_A
Cuts at: 0 1520 1562 1575
Size: 1520 42 13
Fragments arranged by size:
1520 42 13
PmlI CAC'GTG
Cuts at: 0 1065 1575
Size: 1065 510
PpuMI rG'GwC_Cy
Cuts at: 0 1418 1575
Size: 1418 157
PshAI GACnn'nnGTC
Cuts at: 0 705 1575
Size: 705 870
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1528 1560 1575
Size: 1528 32 15
Fragments arranged by size:
1528 32 15
SapI GCTCTTCn'nnn_
Cuts at: 0 1005 1575
Size: 1005 570
SexAI A'CCwGG_T
Cuts at: 0 737 1575
Size: 737 838
SphI G_CATG'C
Cuts at: 0 280 1575
Size: 280 1295
StyI C'CwwG_G
Cuts at: 0 32 383 540 942 1421 1499 1575
Size: 32 351 157 402 479 78 76
Fragments arranged by size:
479 402 351 157 78 76 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 654 940 1575
Size: 654 286 635
Fragments arranged by size:
654 635 286
ZraI GAC'GTC
Cuts at: 0 924 1518 1575
Size: 924 594 57
Fragments arranged by size:
924 594 57
Enzymes that do cut:
AarI AatII AcuI AflIII AgeI AleI AlwNI AvaI
BanI BanII BbsI BbvCI BclI BglI BglII BmgBI
BplI BpmI Bpu10I BsaAI BsaHI BsaWI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrFI
BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI DraIII
EaeI EarI EciI Eco57MI EcoO109I HaeII HincII HindIII
HpaI MmeI MscI MslI MspA1I NcoI NspI PasI
PfoI PmlI PpuMI PshAI PsrI SapI SexAI SphI
StyI XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AhdI AlfI AloI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BcgI BciVI BfrBI BlpI Bme1580I BmrI
BmtI BpuEI BsaI BsaBI BsaXI BsiEI BsiWI BsmBI
BspEI BsrDI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DrdI EagI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PflMI PmeI PpiI PsiI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI