(Linear) (Six-base) MAPSORT of: KIBB8608.seq Check: 301 from: 1 to: 2919 KIBB8608.orf With 182 enzymes: SgfI * July 15, 2010 18:32 .. AccI GT'mk_AC Cuts at: 0 174 2919 Size: 174 2745 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2342 2919 Size: 2342 577 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1379 2919 Size: 1379 1540 AlwNI CAG_nnn'CTG Cuts at: 0 226 917 2412 2484 2919 Size: 226 691 1495 72 435 Fragments arranged by size: 1495 691 435 226 72 ApaLI G'TGCA_C Cuts at: 0 1443 2919 Size: 1443 1476 ApoI r'AATT_y Cuts at: 0 3 312 356 816 1758 2919 Size: 3 309 44 460 942 1161 Fragments arranged by size: 1161 942 460 309 44 3 AseI AT'TA_AT Cuts at: 0 1516 2919 Size: 1516 1403 BanII G_rGCy'C Cuts at: 0 2199 2919 Size: 2199 720 BbsI GAAGACnn'nnnn_ Cuts at: 0 2348 2583 2919 Size: 2348 235 336 Fragments arranged by size: 2348 336 235 BclI T'GATC_A Cuts at: 0 1860 2919 Size: 1860 1059 BlpI GC'TnA_GC Cuts at: 0 1537 2919 Size: 1537 1382 Bme1580I G_kGCm'C Cuts at: 0 1447 1659 2919 Size: 1447 212 1260 Fragments arranged by size: 1447 1260 212 BmrI ACTGGGnnnn_n' Cuts at: 0 989 1832 2919 Size: 989 843 1087 Fragments arranged by size: 1087 989 843 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1406 2065 2879 2919 Size: 1406 659 814 40 Fragments arranged by size: 1406 814 659 40 Bpu10I CC'TnA_GC Cuts at: 0 1387 2919 Size: 1387 1532 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1720 2050 2797 2919 Size: 1720 330 747 122 Fragments arranged by size: 1720 747 330 122 BsaI GGTCTCn'nnnn_ Cuts at: 0 1000 2527 2593 2919 Size: 1000 1527 66 326 Fragments arranged by size: 1527 1000 326 66 BsaAI yAC'GTr Cuts at: 0 559 2919 Size: 559 2360 BsaBI GATnn'nnATC Cuts at: 0 2780 2909 2919 Size: 2780 129 10 Fragments arranged by size: 2780 129 10 BsaHI Gr'CG_yC Cuts at: 0 569 2919 Size: 569 2350 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 677 707 727 757 882 912 1205 1235 Size: 677 30 20 30 125 30 293 30 Cuts at: 1235 2919 Size: 1684 Fragments arranged by size: 1684 677 293 125 30 30 30 30 20 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 421 682 2919 Size: 421 261 2237 Fragments arranged by size: 2237 421 261 BseYI C'CCAG_C Cuts at: 0 44 1823 2919 Size: 44 1779 1096 Fragments arranged by size: 1779 1096 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 815 2919 Size: 815 2104 BsiHKAI G_wGCw'C Cuts at: 0 135 1447 2768 2919 Size: 135 1312 1321 151 Fragments arranged by size: 1321 1312 151 135 BsmI GAATG_Cn' Cuts at: 0 2210 2919 Size: 2210 709 BsmBI CGTCTCn'nnnn_ Cuts at: 0 55 2919 Size: 55 2864 Bsp1286I G_dGCh'C Cuts at: 0 135 1447 1659 2199 2768 2919 Size: 135 1312 212 540 569 151 Fragments arranged by size: 1312 569 540 212 151 135 BspHI T'CATG_A Cuts at: 0 1863 2919 Size: 1863 1056 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2037 2919 Size: 2037 882 BsrDI GCAATG_nn' Cuts at: 0 606 1550 2684 2919 Size: 606 944 1134 235 Fragments arranged by size: 1134 944 606 235 BsrFI r'CCGG_y Cuts at: 0 1098 2919 Size: 1098 1821 BstXI CCAn_nnnn'nTGG Cuts at: 0 2338 2919 Size: 2338 581 BstYI r'GATC_y Cuts at: 0 2019 2111 2817 2892 2904 2919 Size: 2019 92 706 75 12 15 Fragments arranged by size: 2019 706 92 75 15 12 BstZ17I GTA'TAC Cuts at: 0 175 2919 Size: 175 2744 Bsu36I CC'TnA_GG Cuts at: 0 476 2919 Size: 476 2443 BtgI C'CryG_G Cuts at: 0 12 1124 2919 Size: 12 1112 1795 Fragments arranged by size: 1795 1112 12 DraI TTT'AAA Cuts at: 0 461 762 2423 2919 Size: 461 301 1661 496 Fragments arranged by size: 1661 496 461 301 DraIII CAC_nnn'GTG Cuts at: 0 1465 2919 Size: 1465 1454 EaeI y'GGCC_r Cuts at: 0 1275 2771 2919 Size: 1275 1496 148 Fragments arranged by size: 1496 1275 148 EarI CTCTTCn'nnn_ Cuts at: 0 392 1625 1735 1943 2237 2834 2919 Size: 392 1233 110 208 294 597 85 Fragments arranged by size: 1233 597 392 294 208 110 85 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1406 2065 2342 2879 2919 Size: 1406 659 277 537 40 Fragments arranged by size: 1406 659 537 277 40 EcoO109I rG'GnC_Cy Cuts at: 0 472 2919 Size: 472 2447 EcoRV GAT'ATC Cuts at: 0 996 2919 Size: 996 1923 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1845 1877 2919 Size: 1845 32 1042 Fragments arranged by size: 1845 1042 32 HincII GTy'rAC Cuts at: 0 1226 2919 Size: 1226 1693 HindIII A'AGCT_T Cuts at: 0 73 442 2919 Size: 73 369 2477 Fragments arranged by size: 2477 369 73 MfeI C'AATT_G Cuts at: 0 1103 1542 2919 Size: 1103 439 1377 Fragments arranged by size: 1377 1103 439 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 786 1488 2049 2702 2919 Size: 786 702 561 653 217 Fragments arranged by size: 786 702 653 561 217 MscI TGG'CCA Cuts at: 0 1277 2919 Size: 1277 1642 MslI CAynn'nnrTG Cuts at: 0 1123 2061 2919 Size: 1123 938 858 Fragments arranged by size: 1123 938 858 MspA1I CmG'CkG Cuts at: 0 14 428 1273 2289 2664 2919 Size: 14 414 845 1016 375 255 Fragments arranged by size: 1016 845 414 375 255 14 NcoI C'CATG_G Cuts at: 0 1124 2919 Size: 1124 1795 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1370 1402 2919 Size: 1370 32 1517 Fragments arranged by size: 1517 1370 32 PshAI GACnn'nnGTC Cuts at: 0 2089 2919 Size: 2089 830 PsiI TTA'TAA Cuts at: 0 1903 1919 2919 Size: 1903 16 1000 Fragments arranged by size: 1903 1000 16 PstI C_TGCA'G Cuts at: 0 584 2919 Size: 584 2335 PvuII CAG'CTG Cuts at: 0 1273 2289 2664 2919 Size: 1273 1016 375 255 Fragments arranged by size: 1273 1016 375 255 SacII CC_GC'GG Cuts at: 0 15 2919 Size: 15 2904 SapI GCTCTTCn'nnn_ Cuts at: 0 1625 2919 Size: 1625 1294 ScaI AGT'ACT Cuts at: 0 1112 2919 Size: 1112 1807 SfcI C'TryA_G Cuts at: 0 580 862 2464 2919 Size: 580 282 1602 455 Fragments arranged by size: 1602 580 455 282 SmlI C'TyrA_G Cuts at: 0 1699 2029 2812 2919 Size: 1699 330 783 107 Fragments arranged by size: 1699 783 330 107 SnaBI TAC'GTA Cuts at: 0 559 2919 Size: 559 2360 SspI AAT'ATT Cuts at: 0 754 1452 2919 Size: 754 698 1467 Fragments arranged by size: 1467 754 698 StyI C'CwwG_G Cuts at: 0 793 1124 1644 2919 Size: 793 331 520 1275 Fragments arranged by size: 1275 793 520 331 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 499 1384 2551 2919 Size: 499 885 1167 368 Fragments arranged by size: 1167 885 499 368 TatI w'GTAC_w Cuts at: 0 961 1110 2919 Size: 961 149 1809 Fragments arranged by size: 1809 961 149 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 882 914 1205 1237 2919 Size: 882 32 291 32 1682 Fragments arranged by size: 1682 882 291 32 32 XbaI T'CTAG_A Cuts at: 0 2907 2919 Size: 2907 12 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1285 2919 Size: 1285 1634 XmnI GAAnn'nnTTC Cuts at: 0 1031 2919 Size: 1031 1888 Enzymes that do cut: AccI AcuI AlfI AlwNI ApaLI ApoI AseI BanII BbsI BclI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BstXI BstYI BstZ17I Bsu36I BtgI DraI DraIII EaeI EarI Eco57MI EcoO109I EcoRV Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NcoI PpiI PshAI PsiI PstI PvuII SacII SapI ScaI SfcI SmlI SnaBI SspI StyI TaqII TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaI AscI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BfrBI BglI BglII BmgBI BmtI BplI BsaWI BsiEI BsiWI BspEI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BtgZI BtsI ClaI CspCI DrdI EagI EciI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HaeII HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpuMI PspOMI PspXI PsrI PvuI RsrII SacI SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SphI SrfI StuI SwaI Tth111I XhoI XmaI ZraI