################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlnI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HindIII HpaI KpnI KspI MamI MfeI MflI MluI MroI MseI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tru9I Tsp509I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AflIII 122 ACATGT A^C[GA][CT]GT AlwI 744 GATCC GGATC(4/5) BalI 254 TGGCCA TGG^CCA BanI 89 GGCGCC G^G[CT][GA]CC BanII 110 GAGCCC G[GA]GC[CT]^C BbeI 89 GGCGCC GGCGC^C BbsI 645 GAAGAC GAAGAC(2/6) BclI 170 TGATCA T^GATCA BlpI 466 GCTGAGC GC^T[ACGT]AGC Bpu1102I 466 GCTGAGC GC^T[ACGT]AGC BpuAI 645 GAAGAC GAAGAC(2/6) BsaAI 103 TACGTG [CT]AC^GT[GA] BspLU11I 122 ACATGT A^CATGT BsrFI 156 ACCGGC [GA]^CCGG[CT] BstBI 755 TTCGAA TT^CGAA BstF5I 209 GGATG GGATG(2/0) BstXI 554 CCAATACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 466 GCTGAGC GC^T[ACGT]AGC Cfr10I 156 ACCGGC [GA]^CCGG[CT] FbaI 170 TGATCA T^GATCA FokI 209 GGATG GGATG(9/13) HaeII 89 GGCGCC [GA]GCGC^[CT] HincII 688 GTCAAC GT[CT]^[GA]AC HindII 688 GTCAAC GT[CT]^[GA]AC HphI 168 GGTGA GGTGA(8/7) KasI 89 GGCGCC G^GCGCC MluNI 254 TGGCCA TGG^CCA MscI 254 TGGCCA TGG^CCA MslI 735 CATTAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 89 GGCGCC GG^CGCC NspI 122 ACATGT [GA]CATG^[CT] NspV 755 TTCGAA TT^CGAA PvuII 464 CAGCTG CAG^CTG SexAI 26 ACCTGGT A^CC[AT]GGT SfoI 89 GGCGCC GGC^GCC SfuI 755 TTCGAA TT^CGAA SmlI 547 CTCAAG C^T[CT][GA]AG SspI 179 AATATT AAT^ATT TfiI 758 GAATC G^A[AT]TC XcmI 257 CCAACCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccII 147 CGCG CG^CG 344 CGCG AcyI 6 GGCGTC G[GA]^CG[CT]C 89 GGCGCC AfaI 102 GTAC GT^AC 348 GTAC BcnI 78 CCCGG CC^[GC]GG 539 CCCGG BmyI 110 GAGCCC G[AGT]GC[ACT]^C 307 GTGCCC BsaHI 6 GGCGTC G[GA]^CG[CT]C 89 GGCGCC BsaJI 273 CCCTGG C^C[ACGT][ACGT]GG 560 CCCTGG BseRI 234 CTCCTC GAGGAG(10/8) 237 CTCCTC BsmFI 74 GGGAC GGGAC(10/14) 435 GTCCC Bsp1286I 110 GAGCCC G[AGT]GC[ACT]^C 307 GTGCCC BstUI 147 CGCG CG^CG 344 CGCG CfoI 90 GCGC GCG^C 343 GCGC DpnII 171 GATC ^GATC 744 GATC FauI 44 CCCGC CCCGC(4/6) 310 CCCGC HgaI 7 GCGTC GACGC(5/10) 660 GCGTC HhaI 90 GCGC GCG^C 343 GCGC Hin1I 6 GGCGTC G[GA]^CG[CT]C 89 GGCGCC HinP1I 90 GCGC G^CGC 343 GCGC MaeII 104 ACGT A^CGT 407 ACGT MaeIII 336 GTCAC ^GT[ACGT]AC 603 GTCAC MboI 171 GATC ^GATC 744 GATC MvnI 147 CGCG CG^CG 344 CGCG NciI 78 CCCGG CC^[GC]GG 539 CCCGG NdeII 171 GATC ^GATC 744 GATC PflMI 261 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 627 CCAGAACCTGG PleI 385 GACTC GAGTC(4/5) 652 GAGTC PpuMI 74 GGGACCC [GA]G^G[AT]CC[CT] 433 GGGTCCC RsaI 102 GTAC GT^AC 348 GTAC Sau3AI 171 GATC ^GATC 744 GATC TaqI 10 TCGA T^CGA 756 TCGA Tsp45I 336 GTCAC ^GT[GC]AC 603 GTCAC TthHB8I 10 TCGA T^CGA 756 TCGA Van91I 261 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 627 CCAGAACCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AciI AluI AlwNI AspI AvaII BbvI BfaI BpmI BsiYI BslI BsrI BssKI BstNI Cac8I Cfr13I DdeI DraII EaeI EarI EcoO109I EcoRII Fnu4HI HaeIII HapII HinfI HpaII ItaI Ksp632I MaeI MboII MnlI MspA1I MspI MvaI MwoI NlaIII NlaIV PflFI PstI Sau96I ScrFI SfaNI SfcI TseI TspRI Tth111I