(Circular) (Six-base) MAPSORT of: pFN21AE1131.seq Check: 5608 from: 1 to: 5482 pFN21AE1131 5482 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3292 278 Size: 53 83 186 936 1756 2468 Fragments arranged by size: 2468 1756 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2825 1937 Size: 888 4594 Acc65I G'GTAC_C Cuts at: 2803 2803 Size: 5482 AclI AA'CG_TT Cuts at: 1563 3609 3982 1563 Size: 2046 373 3063 Fragments arranged by size: 3063 2046 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2162 2228 2669 3545 4929 5197 1632 Size: 530 66 441 876 1384 268 1917 Fragments arranged by size: 1917 1384 876 530 441 268 66 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5285 AflIII A'CryG_T Cuts at: 1203 2127 4381 5358 1203 Size: 924 2254 977 1327 Fragments arranged by size: 2254 1327 977 924 AgeI A'CCGG_T Cuts at: 4313 4313 Size: 5482 AhdI GACnn_n'nnGTC Cuts at: 4211 4211 Size: 5482 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5482 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5482 AlwNI CAG_nnn'CTG Cuts at: 2140 2272 2402 2605 2638 3225 4797 2140 Size: 132 130 203 33 587 1572 2825 Fragments arranged by size: 2825 1572 587 203 132 130 33 ApaLI G'TGCA_C Cuts at: 3539 4695 3539 Size: 1156 4326 ApoI r'AATT_y Cuts at: 1493 2791 3017 3169 5278 1493 Size: 1298 226 152 2109 1697 Fragments arranged by size: 2109 1697 1298 226 152 AseI AT'TA_AT Cuts at: 160 1032 4036 160 Size: 872 3004 1606 Fragments arranged by size: 3004 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5482 AvaI C'yCGr_G Cuts at: 1870 1943 2807 1870 Size: 73 864 4545 Fragments arranged by size: 4545 864 73 BamHI G'GATC_C Cuts at: 2812 3179 2812 Size: 367 5115 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2094 2803 4258 618 Size: 325 217 627 307 709 1455 1842 Fragments arranged by size: 1842 1455 709 627 325 307 217 BanII G_rGCy'C Cuts at: 1391 2119 2801 1391 Size: 728 682 4072 Fragments arranged by size: 4072 728 682 BbeI G_GCGC'C Cuts at: 2098 2098 Size: 5482 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2657 928 Size: 971 758 3753 Fragments arranged by size: 3753 971 758 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3673 3707 1903 Size: 34 1736 34 3678 Fragments arranged by size: 3678 1736 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3374 4584 3374 Size: 1210 4272 BclI T'GATC_A Cuts at: 1548 2175 1548 Size: 627 4855 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4093 136 Size: 107 122 71 631 605 337 2084 1525 Fragments arranged by size: 2084 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5477 5477 Size: 5482 BlpI GC'TnA_GC Cuts at: 2472 2899 2472 Size: 427 5055 Bme1580I G_kGCm'C Cuts at: 1790 2316 3543 4699 1790 Size: 526 1227 1156 2573 Fragments arranged by size: 2573 1227 1156 526 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5482 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3213 4171 442 Size: 416 53 83 399 24 1796 958 1753 Fragments arranged by size: 1796 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5482 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2042 2120 2448 2492 2660 2690 4142 1239 Size: 803 78 328 44 168 30 1452 2579 Fragments arranged by size: 2579 1452 803 328 168 78 44 30 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2537 3607 4472 4770 2537 Size: 1070 865 298 3249 Fragments arranged by size: 3249 1070 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4145 882 Size: 617 2646 2219 Fragments arranged by size: 2646 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2110 3273 493 Size: 711 906 1163 2702 Fragments arranged by size: 2702 1163 906 711 BsaBI GATnn'nnATC Cuts at: 5476 5476 Size: 5482 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 2095 3289 3671 Size: 53 83 186 936 479 83 1194 382 Cuts at: 3671 4306 275 Size: 635 1451 Fragments arranged by size: 1451 1194 936 635 479 382 186 83 83 53 BsaWI w'CCGG_w Cuts at: 3915 4313 4587 4734 5145 3915 Size: 398 274 147 411 4252 Fragments arranged by size: 4252 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5257 Fragments arranged by size: 5257 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2230 2233 1186 Size: 1044 3 4435 Fragments arranged by size: 4435 1044 3 BseYI C'CCAG_C Cuts at: 2442 4685 5181 2442 Size: 2243 496 2743 Fragments arranged by size: 2743 2243 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4909 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2932 3693 4721 1540 Size: 275 186 931 761 1028 2301 Fragments arranged by size: 2301 1028 931 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3543 3628 4699 3543 Size: 85 1071 4326 Fragments arranged by size: 4326 1071 85 BsmI GAATG_Cn' Cuts at: 2993 3086 2993 Size: 93 5389 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2119 2316 2801 3543 3628 4699 1391 Size: 399 329 197 485 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 485 399 329 197 85 BspEI T'CCGG_A Cuts at: 5145 5145 Size: 5482 BspHI T'CATG_A Cuts at: 3371 5101 3371 Size: 1730 3752 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2837 844 Size: 1993 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3369 1394 Size: 429 1546 3507 Fragments arranged by size: 3507 1546 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3977 4151 65 Size: 1183 2729 174 1396 Fragments arranged by size: 2729 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2161 4126 4313 1700 Size: 110 351 1965 187 2869 Fragments arranged by size: 2869 1965 351 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5482 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5482 BssSI C'ACGA_G Cuts at: 3542 4554 3542 Size: 1012 4470 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5482 BstBI TT'CG_AA Cuts at: 2761 4286 2761 Size: 1525 3957 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5482 BstXI CCAn_nnnn'nTGG Cuts at: 2566 2566 Size: 5482 BstYI r'GATC_y Cuts at: 1972 2812 3179 3564 3581 5033 5119 5131 5477 Size: 840 367 385 17 1452 86 12 346 Cuts at: 5477 1972 Size: 1977 Fragments arranged by size: 1977 1452 840 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3991 Fragments arranged by size: 3991 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2075 487 Size: 683 56 849 3894 Fragments arranged by size: 3894 849 683 56 BtsI GCAGTG_nn' Cuts at: 2998 3792 3812 2998 Size: 794 20 4668 Fragments arranged by size: 4668 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5482 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5447 DraI TTT'AAA Cuts at: 2786 2786 Size: 5482 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4489 809 Size: 507 3173 1802 Fragments arranged by size: 3173 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2259 2281 2568 Size: 54 1425 50 275 18 429 22 287 Cuts at: 2568 2929 3818 5447 8 Size: 361 889 1629 43 Fragments arranged by size: 1629 1425 889 429 361 287 275 54 50 43 22 18 EagI C'GGCC_G Cuts at: 1537 1812 2929 1537 Size: 275 1117 4090 Fragments arranged by size: 4090 1117 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2414 2520 2759 3412 1353 Size: 1061 106 239 653 3423 Fragments arranged by size: 3423 1061 653 239 106 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2537 2642 4069 4443 4589 387 Size: 1064 138 948 105 1427 374 146 1280 Fragments arranged by size: 1427 1280 1064 948 374 146 138 105 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2120 2162 2228 2448 2492 2660 Size: 393 410 78 42 66 220 44 168 Cuts at: 2660 2669 2690 3545 4142 4929 5197 1239 Size: 9 21 855 597 787 268 1524 Fragments arranged by size: 1524 855 787 597 410 393 268 220 168 78 66 44 42 21 9 EcoO109I rG'GnC_Cy Cuts at: 2080 2137 2439 2080 Size: 57 302 5123 Fragments arranged by size: 5123 302 57 EcoRI G'AATT_C Cuts at: 2791 3169 2791 Size: 378 5104 FspI TGC'GCA Cuts at: 3988 3988 Size: 5482 HaeII r_GCGC'y Cuts at: 1678 2098 4629 1678 Size: 420 2531 2531 Fragments arranged by size: 2531 2531 420 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2317 2349 2447 2479 4123 Size: 32 135 32 745 32 98 32 1644 Cuts at: 4123 4155 4197 4229 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1644 745 135 98 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2695 2826 3072 669 Size: 988 281 757 131 246 3079 Fragments arranged by size: 3079 988 757 281 246 131 HindIII A'AGCT_T Cuts at: 748 748 Size: 5482 HpaI GTT'AAC Cuts at: 3072 3072 Size: 5482 KasI G'GCGC_C Cuts at: 2094 2094 Size: 5482 KpnI G_GTAC'C Cuts at: 2807 2807 Size: 5482 MfeI C'AATT_G Cuts at: 3081 3081 Size: 5482 MluI A'CGCG_T Cuts at: 5358 5358 Size: 5482 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4596 4780 1305 Size: 3291 184 2007 Fragments arranged by size: 3291 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2261 10 Size: 54 1425 343 429 3231 Fragments arranged by size: 3231 1425 429 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2744 3440 3799 3958 518 Size: 648 1578 696 359 159 2042 Fragments arranged by size: 2042 1578 696 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2052 2069 2471 2898 3575 4723 4968 1606 Size: 446 17 402 427 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 446 427 402 245 17 NarI GG'CG_CC Cuts at: 2095 2095 Size: 5482 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3991 Fragments arranged by size: 3991 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5482 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5482 NotI GC'GGCC_GC Cuts at: 2929 2929 Size: 5482 NspI r_CATG'y Cuts at: 1132 1229 2131 2840 4385 1132 Size: 97 902 709 1545 2229 Fragments arranged by size: 2229 1545 902 709 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5482 PciI A'CATG_T Cuts at: 2127 4381 2127 Size: 2254 3228 PflMI CCAn_nnn'nTGG Cuts at: 1108 2272 2638 1108 Size: 1164 366 3952 Fragments arranged by size: 3952 1164 366 PfoI T'CCnGG_A Cuts at: 2189 2508 2189 Size: 319 5163 PmeI GTTT'AAAC Cuts at: 2786 2786 Size: 5482 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3551 3583 5064 5096 3551 Size: 32 1481 32 3937 Fragments arranged by size: 3937 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2080 2439 2080 Size: 359 5123 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5482 PsiI TTA'TAA Cuts at: 3052 3052 Size: 5482 PstI C_TGCA'G Cuts at: 830 2340 2398 2493 2834 830 Size: 1510 58 95 341 3478 Fragments arranged by size: 3478 1510 341 95 58 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5482 PvuII CAG'CTG Cuts at: 2471 2471 Size: 5482 SalI G'TCGA_C Cuts at: 1936 2824 1936 Size: 888 4594 SanDI GG'GwC_CC Cuts at: 2080 2439 2080 Size: 359 5123 SbfI CC_TGCA'GG Cuts at: 2834 2834 Size: 5482 ScaI AGT'ACT Cuts at: 3730 5442 3730 Size: 1712 3770 SexAI A'CCwGG_T Cuts at: 2031 2031 Size: 5482 SfcI C'TryA_G Cuts at: 826 1045 2336 2394 2489 2830 3965 4646 4837 Size: 219 1291 58 95 341 1135 681 191 Cuts at: 4837 826 Size: 1471 Fragments arranged by size: 1471 1291 1135 681 341 219 191 95 58 SfoI GGC'GCC Cuts at: 2096 2096 Size: 5482 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5482 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5482 SmaI CCC'GGG Cuts at: 1872 2809 1872 Size: 937 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2552 3586 4487 4749 820 Size: 197 926 609 1034 901 262 1553 Fragments arranged by size: 1553 1034 926 901 609 262 197 SnaBI TAC'GTA Cuts at: 493 3273 493 Size: 2780 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5482 SphI G_CATG'C Cuts at: 1132 2840 1132 Size: 1708 3774 SspI AAT'ATT Cuts at: 5 52 2186 3406 5 Size: 47 2134 1220 2081 Fragments arranged by size: 2134 2081 1220 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5482 StyI C'CwwG_G Cuts at: 513 1065 2004 2921 513 Size: 552 939 917 3074 Fragments arranged by size: 3074 939 917 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3509 3526 3679 3864 4309 325 Size: 1255 1929 17 153 185 445 1498 Fragments arranged by size: 1929 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3728 5440 96 Size: 274 80 33 51 1443 1751 1712 138 Fragments arranged by size: 1751 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5442 5474 5442 Size: 32 5450 Tth111I GACn'n_nGTC Cuts at: 1316 2483 2605 2656 1316 Size: 1167 122 51 4142 Fragments arranged by size: 4142 1167 122 51 XbaI T'CTAG_A Cuts at: 2818 2818 Size: 5482 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2269 2269 Size: 5482 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5482 XmaI C'CCGG_G Cuts at: 1870 2807 1870 Size: 937 4545 XmnI GAAnn'nnTTC Cuts at: 3611 3611 Size: 5482 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3290 276 Size: 53 83 186 936 1756 2468 Fragments arranged by size: 2468 1756 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SrfI SwaI