Restriction map for
pFN21AE1131
(Linear) (Six-base) MAPSORT of: KIEE1131.seq Check: 1816 from: 1 to: 777
KIEE1131.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 223 664 777
Size: 157 66 441 113
Fragments arranged by size:
441 157 113 66
AflIII A'CryG_T
Cuts at: 0 122 777
Size: 122 655
AlwNI CAG_nnn'CTG
Cuts at: 0 135 267 397 600 633 777
Size: 135 132 130 203 33 144
Fragments arranged by size:
203 144 135 132 130 33
BanI G'GyrC_C
Cuts at: 0 89 777
Size: 89 688
BanII G_rGCy'C
Cuts at: 0 114 777
Size: 114 663
BbeI G_GCGC'C
Cuts at: 0 93 777
Size: 93 684
BbsI GAAGACnn'nnnn_
Cuts at: 0 652 777
Size: 652 125
BclI T'GATC_A
Cuts at: 0 170 777
Size: 170 607
BlpI GC'TnA_GC
Cuts at: 0 467 777
Size: 467 310
Bme1580I G_kGCm'C
Cuts at: 0 311 777
Size: 311 466
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 115 443 487 655 685 777
Size: 37 78 328 44 168 30 92
Fragments arranged by size:
328 168 92 78 44 37 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 777
Size: 532 245
BsaAI yAC'GTr
Cuts at: 0 105 777
Size: 105 672
BsaHI Gr'CG_yC
Cuts at: 0 7 90 777
Size: 7 83 687
Fragments arranged by size:
687 83 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 225 228 777
Size: 225 3 549
Fragments arranged by size:
549 225 3
BseYI C'CCAG_C
Cuts at: 0 437 777
Size: 437 340
Bsp1286I G_dGCh'C
Cuts at: 0 114 311 777
Size: 114 197 466
Fragments arranged by size:
466 197 114
BsrFI r'CCGG_y
Cuts at: 0 156 777
Size: 156 621
BstBI TT'CG_AA
Cuts at: 0 756 777
Size: 756 21
BstXI CCAn_nnnn'nTGG
Cuts at: 0 561 777
Size: 561 216
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 70 777
Size: 70 707
EaeI y'GGCC_r
Cuts at: 0 254 276 563 777
Size: 254 22 287 214
Fragments arranged by size:
287 254 214 22
EarI CTCTTCn'nnn_
Cuts at: 0 409 515 754 777
Size: 409 106 239 23
Fragments arranged by size:
409 239 106 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 532 637 777
Size: 532 105 140
Fragments arranged by size:
532 140 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 115 157 223 443 487 655 664
Size: 37 78 42 66 220 44 168 9
Cuts at: 664 685 777
Size: 21 92
Fragments arranged by size:
220 168 92 78 66 44 42 37
21 9
EcoO109I rG'GnC_Cy
Cuts at: 0 75 132 434 777
Size: 75 57 302 343
Fragments arranged by size:
343 302 75 57
HaeII r_GCGC'y
Cuts at: 0 93 777
Size: 93 684
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 312 344 442 474 777
Size: 312 32 98 32 303
Fragments arranged by size:
312 303 98 32 32
HincII GTy'rAC
Cuts at: 0 690 777
Size: 690 87
KasI G'GCGC_C
Cuts at: 0 89 777
Size: 89 688
MscI TGG'CCA
Cuts at: 0 256 777
Size: 256 521
MslI CAynn'nnrTG
Cuts at: 0 739 777
Size: 739 38
MspA1I CmG'CkG
Cuts at: 0 47 64 466 777
Size: 47 17 402 311
Fragments arranged by size:
402 311 47 17
NarI GG'CG_CC
Cuts at: 0 90 777
Size: 90 687
NspI r_CATG'y
Cuts at: 0 126 777
Size: 126 651
PciI A'CATG_T
Cuts at: 0 122 777
Size: 122 655
PflMI CCAn_nnn'nTGG
Cuts at: 0 267 633 777
Size: 267 366 144
Fragments arranged by size:
366 267 144
PfoI T'CCnGG_A
Cuts at: 0 184 503 777
Size: 184 319 274
Fragments arranged by size:
319 274 184
PpuMI rG'GwC_Cy
Cuts at: 0 75 434 777
Size: 75 359 343
Fragments arranged by size:
359 343 75
PstI C_TGCA'G
Cuts at: 0 335 393 488 777
Size: 335 58 95 289
Fragments arranged by size:
335 289 95 58
PvuII CAG'CTG
Cuts at: 0 466 777
Size: 466 311
SanDI GG'GwC_CC
Cuts at: 0 75 434 777
Size: 75 359 343
Fragments arranged by size:
359 343 75
SexAI A'CCwGG_T
Cuts at: 0 26 777
Size: 26 751
SfcI C'TryA_G
Cuts at: 0 331 389 484 777
Size: 331 58 95 293
Fragments arranged by size:
331 293 95 58
SfoI GGC'GCC
Cuts at: 0 91 777
Size: 91 686
SmlI C'TyrA_G
Cuts at: 0 547 777
Size: 547 230
SspI AAT'ATT
Cuts at: 0 181 777
Size: 181 596
Tth111I GACn'n_nGTC
Cuts at: 0 478 600 651 777
Size: 478 122 51 126
Fragments arranged by size:
478 126 122 51
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 264 777
Size: 264 513
Enzymes that do cut:
AcuI AflIII AlwNI BanI BanII BbeI BbsI BclI
BlpI Bme1580I BpmI BpuEI BsaAI BsaHI BseRI BseYI
Bsp1286I BsrFI BstBI BstXI BtgZI EaeI EarI EciI
Eco57MI EcoO109I HaeII Hin4I HincII KasI MscI MslI
MspA1I NarI NspI PciI PflMI PfoI PpuMI PstI
PvuII SanDI SexAI SfcI SfoI SmlI SspI Tth111I
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI
BciVI BfrBI BglI BglII BmgBI BmrI BmtI BplI
Bpu10I BsaI BsaBI BsaWI BsaXI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstEII BstYI BstZ17I Bsu36I
BtgI BtsI ClaI CspCI DraI DraIII DrdI EagI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI MmeI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SapI SbfI ScaI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI StyI SwaI TaqII TatI TstI XbaI XhoI
XmaI XmnI ZraI