(Circular) (Six-base) MAPSORT of: pFN21AE0551.seq Check: 2433 from: 1 to: 5566 pFN21AE0551 5566 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3376 278 Size: 53 83 186 936 1840 2468 Fragments arranged by size: 2468 1840 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2187 2205 2909 1937 Size: 250 18 704 4594 Fragments arranged by size: 4594 704 250 18 Acc65I G'GTAC_C Cuts at: 2887 2887 Size: 5566 AclI AA'CG_TT Cuts at: 1563 2267 3693 4066 1563 Size: 704 1426 373 3063 Fragments arranged by size: 3063 1426 704 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3629 5013 5281 1632 Size: 1997 1384 268 1917 Fragments arranged by size: 1997 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5369 AflIII A'CryG_T Cuts at: 1203 2598 2802 4465 5442 1203 Size: 1395 204 1663 977 1327 Fragments arranged by size: 1663 1395 1327 977 204 AgeI A'CCGG_T Cuts at: 4397 4397 Size: 5566 AhdI GACnn_n'nnGTC Cuts at: 4295 4295 Size: 5566 AleI CACnn'nnGTG Cuts at: 1166 2807 1166 Size: 1641 3925 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2357 1184 Size: 1173 4393 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2477 2509 2477 Size: 32 5534 AlwNI CAG_nnn'CTG Cuts at: 2389 2545 3309 4881 2389 Size: 156 764 1572 3074 Fragments arranged by size: 3074 1572 764 156 ApaI G_GGCC'C Cuts at: 2023 2023 Size: 5566 ApaLI G'TGCA_C Cuts at: 3623 4779 3623 Size: 1156 4410 ApoI r'AATT_y Cuts at: 1493 2875 3101 3253 5362 1493 Size: 1382 226 152 2109 1697 Fragments arranged by size: 2109 1697 1382 226 152 AseI AT'TA_AT Cuts at: 160 1032 4120 160 Size: 872 3088 1606 Fragments arranged by size: 3088 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5566 AvaI C'yCGr_G Cuts at: 1870 1943 2891 1870 Size: 73 948 4545 Fragments arranged by size: 4545 948 73 BamHI G'GATC_C Cuts at: 2437 2896 3263 2437 Size: 459 367 4740 Fragments arranged by size: 4740 459 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2174 2414 2687 2887 4342 Size: 325 217 627 387 240 273 200 1455 Cuts at: 4342 618 Size: 1842 Fragments arranged by size: 1842 1455 627 387 325 273 240 217 200 BanII G_rGCy'C Cuts at: 1391 2023 2264 2654 2885 1391 Size: 632 241 390 231 4072 Fragments arranged by size: 4072 632 390 241 231 BbeI G_GCGC'C Cuts at: 2691 2691 Size: 5566 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2787 928 Size: 971 888 3707 Fragments arranged by size: 3707 971 888 BbvCI CC'TCA_GC Cuts at: 2699 2838 2699 Size: 139 5427 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2454 2488 3757 3791 1903 Size: 34 50 34 433 34 1269 34 3678 Fragments arranged by size: 3678 1269 433 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3458 4668 3458 Size: 1210 4356 BclI T'GATC_A Cuts at: 1548 1548 Size: 5566 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2027 2611 4177 Size: 107 122 71 631 605 355 584 1566 Cuts at: 4177 136 Size: 1525 Fragments arranged by size: 1566 1525 631 605 584 355 122 107 71 BglII A'GATC_T Cuts at: 5561 5561 Size: 5566 BlpI GC'TnA_GC Cuts at: 2983 2983 Size: 5566 Bme1580I G_kGCm'C Cuts at: 1790 2023 3627 4783 1790 Size: 233 1604 1156 2573 Fragments arranged by size: 2573 1604 1156 233 BmgBI CAC'GTC Cuts at: 1318 2805 1318 Size: 1487 4079 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2267 3297 4255 Size: 416 53 83 399 24 850 1030 958 Cuts at: 4255 442 Size: 1753 Fragments arranged by size: 1753 1030 958 850 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5566 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2843 4226 1239 Size: 1604 1383 2579 Fragments arranged by size: 2579 1604 1383 Bpu10I CC'TnA_GC Cuts at: 2699 2838 2699 Size: 139 5427 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3691 4556 4854 3691 Size: 865 298 4403 Fragments arranged by size: 4403 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4229 882 Size: 617 2730 2219 Fragments arranged by size: 2730 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2601 3357 493 Size: 711 1397 756 2702 Fragments arranged by size: 2702 1397 756 711 BsaBI GATnn'nnATC Cuts at: 2633 5560 2633 Size: 2927 2639 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2688 3373 3755 4390 Size: 53 83 186 936 1155 685 382 635 Cuts at: 4390 275 Size: 1451 Fragments arranged by size: 1451 1155 936 685 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3999 4397 4671 4818 5229 3999 Size: 398 274 147 411 4336 Fragments arranged by size: 4336 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2281 2311 2477 2507 1178 Size: 30 165 30 878 30 166 30 4237 Fragments arranged by size: 4237 878 166 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5566 BseYI C'CCAG_C Cuts at: 2345 2779 4769 5265 2345 Size: 434 1990 496 2646 Fragments arranged by size: 2646 1990 496 434 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4993 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2595 3016 3777 4805 1540 Size: 275 186 594 421 761 1028 2301 Fragments arranged by size: 2301 1028 761 594 421 275 186 BsiHKAI G_wGCw'C Cuts at: 3627 3712 4783 3627 Size: 85 1071 4410 Fragments arranged by size: 4410 1071 85 BsmI GAATG_Cn' Cuts at: 2230 2398 3077 3170 2230 Size: 168 679 93 4626 Fragments arranged by size: 4626 679 168 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2023 2264 2654 2885 3627 3712 4783 Size: 399 233 241 390 231 742 85 1071 Cuts at: 4783 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 390 241 233 231 85 BspEI T'CCGG_A Cuts at: 5229 5229 Size: 5566 BspHI T'CATG_A Cuts at: 3455 5185 3455 Size: 1730 3836 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2073 2349 2435 2921 844 Size: 1229 276 86 486 3489 Fragments arranged by size: 3489 1229 486 276 86 BsrBI CCG'CTC Cuts at: 1394 1823 2014 2619 3453 1394 Size: 429 191 605 834 3507 Fragments arranged by size: 3507 834 605 429 191 BsrDI GCAATG_nn' Cuts at: 65 1248 2691 4061 4235 65 Size: 1183 1443 1370 174 1396 Fragments arranged by size: 1443 1396 1370 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2610 4210 4397 1700 Size: 110 800 1600 187 2869 Fragments arranged by size: 2869 1600 800 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5566 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5566 BssSI C'ACGA_G Cuts at: 2180 2705 3626 4638 2180 Size: 525 921 1012 3108 Fragments arranged by size: 3108 1012 921 525 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5566 BstBI TT'CG_AA Cuts at: 4370 4370 Size: 5566 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5566 BstXI CCAn_nnnn'nTGG Cuts at: 2527 2779 2527 Size: 252 5314 BstYI r'GATC_y Cuts at: 1972 2385 2437 2896 3263 3648 3665 5117 5203 Size: 413 52 459 367 385 17 1452 86 Cuts at: 5203 5215 5561 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 459 413 385 367 346 86 52 17 12 BtgI C'CryG_G Cuts at: 513 1065 2481 2520 513 Size: 552 1416 39 3559 Fragments arranged by size: 3559 1416 552 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4827 Fragments arranged by size: 4827 683 56 BtsI GCAGTG_nn' Cuts at: 2341 3082 3876 3896 2341 Size: 741 794 20 4011 Fragments arranged by size: 4011 794 741 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5566 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5531 DraI TTT'AAA Cuts at: 2870 2870 Size: 5566 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2548 4573 809 Size: 507 1232 2025 1802 Fragments arranged by size: 2025 1802 1232 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2079 2603 3013 Size: 54 1425 50 275 18 249 524 410 Cuts at: 3013 3902 5531 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 524 410 275 249 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3013 1537 Size: 275 1201 4090 Fragments arranged by size: 4090 1201 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3496 1353 Size: 2143 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2629 4153 4527 4673 387 Size: 1064 138 1040 1524 374 146 1280 Fragments arranged by size: 1524 1280 1064 1040 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2843 3629 4226 5013 5281 1239 Size: 393 1211 786 597 787 268 1524 Fragments arranged by size: 1524 1211 787 786 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2020 2164 2297 2424 2452 2784 2020 Size: 144 133 127 28 332 4802 Fragments arranged by size: 4802 332 144 133 127 28 EcoRI G'AATT_C Cuts at: 2875 3253 2875 Size: 378 5188 FspI TGC'GCA Cuts at: 4072 4072 Size: 5566 HaeII r_GCGC'y Cuts at: 1678 2691 4713 1678 Size: 1013 2022 2531 Fragments arranged by size: 2531 2022 1013 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2281 2313 2613 2645 4207 Size: 32 135 32 709 32 300 32 1562 Cuts at: 4207 4239 4281 4313 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1562 709 300 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2206 2910 3156 669 Size: 988 281 268 704 246 3079 Fragments arranged by size: 3079 988 704 281 268 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5566 HpaI GTT'AAC Cuts at: 3156 3156 Size: 5566 KasI G'GCGC_C Cuts at: 2687 2687 Size: 5566 KpnI G_GTAC'C Cuts at: 2891 2891 Size: 5566 MfeI C'AATT_G Cuts at: 3165 3165 Size: 5566 MluI A'CGCG_T Cuts at: 5442 5442 Size: 5566 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2532 4680 4864 1305 Size: 1227 2148 184 2007 Fragments arranged by size: 2148 2007 1227 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2081 2605 10 Size: 54 1425 343 249 524 2971 Fragments arranged by size: 2971 1425 524 343 249 54 MslI CAynn'nnrTG Cuts at: 518 1166 2807 3524 3883 4042 518 Size: 648 1641 717 359 159 2042 Fragments arranged by size: 2042 1641 717 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2982 3659 4807 5052 1606 Size: 1376 677 1148 245 2120 Fragments arranged by size: 2120 1376 1148 677 245 NaeI GCC'GGC Cuts at: 2612 2612 Size: 5566 NarI GG'CG_CC Cuts at: 2688 2688 Size: 5566 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5014 NdeI CA'TA_TG Cuts at: 387 387 Size: 5566 NgoMIV G'CCGG_C Cuts at: 2610 2610 Size: 5566 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5566 NotI GC'GGCC_GC Cuts at: 3013 3013 Size: 5566 NspI r_CATG'y Cuts at: 1132 1229 2149 2924 4469 1132 Size: 97 920 775 1545 2229 Fragments arranged by size: 2229 1545 920 775 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5566 PciI A'CATG_T Cuts at: 4465 4465 Size: 5566 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5566 PfoI T'CCnGG_A Cuts at: 2219 2859 2219 Size: 640 4926 PmeI GTTT'AAAC Cuts at: 2870 2870 Size: 5566 PmlI CAC'GTG Cuts at: 2601 2601 Size: 5566 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2477 2509 3635 3667 5148 5180 2477 Size: 32 1126 32 1481 32 2863 Fragments arranged by size: 2863 1481 1126 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2297 2424 2297 Size: 127 5439 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5566 PsiI TTA'TAA Cuts at: 3136 3136 Size: 5566 PspOMI G'GGCC_C Cuts at: 2019 2019 Size: 5566 PstI C_TGCA'G Cuts at: 830 2070 2336 2918 830 Size: 1240 266 582 3478 Fragments arranged by size: 3478 1240 582 266 PvuI CG_AT'CG Cuts at: 2001 2595 2001 Size: 594 4972 SalI G'TCGA_C Cuts at: 1936 2204 2908 1936 Size: 268 704 4594 Fragments arranged by size: 4594 704 268 SbfI CC_TGCA'GG Cuts at: 2918 2918 Size: 5566 ScaI AGT'ACT Cuts at: 3814 5526 3814 Size: 1712 3854 SexAI A'CCwGG_T Cuts at: 2400 2400 Size: 5566 SfcI C'TryA_G Cuts at: 826 1045 2066 2332 2914 4049 4730 4921 826 Size: 219 1021 266 582 1135 681 191 1471 Fragments arranged by size: 1471 1135 1021 681 582 266 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2027 2027 Size: 5566 SfoI GGC'GCC Cuts at: 2689 2689 Size: 5566 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5566 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5566 SmaI CCC'GGG Cuts at: 1872 2893 1872 Size: 1021 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3670 4571 4833 820 Size: 197 926 1727 901 262 1553 Fragments arranged by size: 1727 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3357 493 Size: 2864 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5566 SphI G_CATG'C Cuts at: 1132 2924 1132 Size: 1792 3774 SspI AAT'ATT Cuts at: 5 52 3490 5 Size: 47 3438 2081 Fragments arranged by size: 3438 2081 47 StuI AGG'CCT Cuts at: 1517 2378 1517 Size: 861 4705 StyI C'CwwG_G Cuts at: 513 1065 2127 2373 3005 513 Size: 552 1062 246 632 3074 Fragments arranged by size: 3074 1062 632 552 246 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2192 2402 3593 3610 3763 3948 4393 Size: 1255 612 210 1191 17 153 185 445 Cuts at: 4393 325 Size: 1498 Fragments arranged by size: 1498 1255 1191 612 445 210 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2487 3812 5524 Size: 274 80 33 51 1443 510 1325 1712 Cuts at: 5524 96 Size: 138 Fragments arranged by size: 1712 1443 1325 510 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5526 5558 5526 Size: 32 5534 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5566 XbaI T'CTAG_A Cuts at: 2902 2902 Size: 5566 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2125 2254 2125 Size: 129 5437 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5566 XmaI C'CCGG_G Cuts at: 1870 2891 1870 Size: 1021 4545 XmnI GAAnn'nnTTC Cuts at: 3695 3695 Size: 5566 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3374 276 Size: 53 83 186 936 1840 2468 Fragments arranged by size: 2468 1840 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SrfI SwaI