Restriction map for
pFN21AE0551
(Linear) (Six-base) MAPSORT of: KIEE0551.seq Check: 1001 from: 1 to: 861
KIEE0551.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 0 182 200 861
Size: 182 18 661
Fragments arranged by size:
661 182 18
AclI AA'CG_TT
Cuts at: 0 262 861
Size: 262 599
AflIII A'CryG_T
Cuts at: 0 593 797 861
Size: 593 204 64
Fragments arranged by size:
593 204 64
AleI CACnn'nnGTG
Cuts at: 0 802 861
Size: 802 59
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 352 861
Size: 352 509
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 472 504 861
Size: 472 32 357
Fragments arranged by size:
472 357 32
AlwNI CAG_nnn'CTG
Cuts at: 0 384 540 861
Size: 384 156 321
Fragments arranged by size:
384 321 156
ApaI G_GGCC'C
Cuts at: 0 18 861
Size: 18 843
BamHI G'GATC_C
Cuts at: 0 432 861
Size: 432 429
BanI G'GyrC_C
Cuts at: 0 169 409 682 861
Size: 169 240 273 179
Fragments arranged by size:
273 240 179 169
BanII G_rGCy'C
Cuts at: 0 18 259 649 861
Size: 18 241 390 212
Fragments arranged by size:
390 241 212 18
BbeI G_GCGC'C
Cuts at: 0 686 861
Size: 686 175
BbsI GAAGACnn'nnnn_
Cuts at: 0 782 861
Size: 782 79
BbvCI CC'TCA_GC
Cuts at: 0 694 833 861
Size: 694 139 28
Fragments arranged by size:
694 139 28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 449 483 861
Size: 449 34 378
Fragments arranged by size:
449 378 34
BglI GCCn_nnn'nGGC
Cuts at: 0 22 606 861
Size: 22 584 255
Fragments arranged by size:
584 255 22
Bme1580I G_kGCm'C
Cuts at: 0 18 861
Size: 18 843
BmgBI CAC'GTC
Cuts at: 0 800 861
Size: 800 61
BmrI ACTGGGnnnn_n'
Cuts at: 0 262 861
Size: 262 599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 838 861
Size: 838 23
Bpu10I CC'TnA_GC
Cuts at: 0 694 833 861
Size: 694 139 28
Fragments arranged by size:
694 139 28
BsaAI yAC'GTr
Cuts at: 0 596 861
Size: 596 265
BsaBI GATnn'nnATC
Cuts at: 0 628 861
Size: 628 233
BsaHI Gr'CG_yC
Cuts at: 0 683 861
Size: 683 178
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 276 306 472 502 861
Size: 276 30 166 30 359
Fragments arranged by size:
359 276 166 30 30
BseYI C'CCAG_C
Cuts at: 0 340 774 861
Size: 340 434 87
Fragments arranged by size:
434 340 87
BsiEI CG_ry'CG
Cuts at: 0 590 861
Size: 590 271
BsmI GAATG_Cn'
Cuts at: 0 225 393 861
Size: 225 168 468
Fragments arranged by size:
468 225 168
Bsp1286I G_dGCh'C
Cuts at: 0 18 259 649 861
Size: 18 241 390 212
Fragments arranged by size:
390 241 212 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 68 344 430 861
Size: 68 276 86 431
Fragments arranged by size:
431 276 86 68
BsrBI CCG'CTC
Cuts at: 0 9 614 861
Size: 9 605 247
Fragments arranged by size:
605 247 9
BsrDI GCAATG_nn'
Cuts at: 0 686 861
Size: 686 175
BsrFI r'CCGG_y
Cuts at: 0 605 861
Size: 605 256
BssSI C'ACGA_G
Cuts at: 0 175 700 861
Size: 175 525 161
Fragments arranged by size:
525 175 161
BstXI CCAn_nnnn'nTGG
Cuts at: 0 522 774 861
Size: 522 252 87
Fragments arranged by size:
522 252 87
BstYI r'GATC_y
Cuts at: 0 380 432 861
Size: 380 52 429
Fragments arranged by size:
429 380 52
BtgI C'CryG_G
Cuts at: 0 476 515 861
Size: 476 39 346
Fragments arranged by size:
476 346 39
BtsI GCAGTG_nn'
Cuts at: 0 336 861
Size: 336 525
DrdI GACnn_nn'nnGTC
Cuts at: 0 543 861
Size: 543 318
EaeI y'GGCC_r
Cuts at: 0 74 598 861
Size: 74 524 263
Fragments arranged by size:
524 263 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 624 861
Size: 624 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 838 861
Size: 838 23
EcoO109I rG'GnC_Cy
Cuts at: 0 15 159 292 419 447 779 861
Size: 15 144 133 127 28 332 82
Fragments arranged by size:
332 144 133 127 82 28 15
HaeII r_GCGC'y
Cuts at: 0 686 861
Size: 686 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 276 308 608 640 861
Size: 276 32 300 32 221
Fragments arranged by size:
300 276 221 32 32
HincII GTy'rAC
Cuts at: 0 201 861
Size: 201 660
KasI G'GCGC_C
Cuts at: 0 682 861
Size: 682 179
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 861
Size: 527 334
MscI TGG'CCA
Cuts at: 0 76 600 861
Size: 76 524 261
Fragments arranged by size:
524 261 76
MslI CAynn'nnrTG
Cuts at: 0 802 861
Size: 802 59
NaeI GCC'GGC
Cuts at: 0 607 861
Size: 607 254
NarI GG'CG_CC
Cuts at: 0 683 861
Size: 683 178
NgoMIV G'CCGG_C
Cuts at: 0 605 861
Size: 605 256
NspI r_CATG'y
Cuts at: 0 144 861
Size: 144 717
PfoI T'CCnGG_A
Cuts at: 0 214 854 861
Size: 214 640 7
Fragments arranged by size:
640 214 7
PmlI CAC'GTG
Cuts at: 0 596 861
Size: 596 265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 472 504 861
Size: 472 32 357
Fragments arranged by size:
472 357 32
PpuMI rG'GwC_Cy
Cuts at: 0 292 419 861
Size: 292 127 442
Fragments arranged by size:
442 292 127
PspOMI G'GGCC_C
Cuts at: 0 14 861
Size: 14 847
PstI C_TGCA'G
Cuts at: 0 65 331 861
Size: 65 266 530
Fragments arranged by size:
530 266 65
PvuI CG_AT'CG
Cuts at: 0 590 861
Size: 590 271
SalI G'TCGA_C
Cuts at: 0 199 861
Size: 199 662
SexAI A'CCwGG_T
Cuts at: 0 395 861
Size: 395 466
SfcI C'TryA_G
Cuts at: 0 61 327 861
Size: 61 266 534
Fragments arranged by size:
534 266 61
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 22 861
Size: 22 839
SfoI GGC'GCC
Cuts at: 0 684 861
Size: 684 177
StuI AGG'CCT
Cuts at: 0 373 861
Size: 373 488
StyI C'CwwG_G
Cuts at: 0 122 368 861
Size: 122 246 493
Fragments arranged by size:
493 246 122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 187 397 861
Size: 187 210 464
Fragments arranged by size:
464 210 187
TatI w'GTAC_w
Cuts at: 0 482 861
Size: 482 379
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 120 249 861
Size: 120 129 612
Fragments arranged by size:
612 129 120
Enzymes that do cut:
AccI AclI AflIII AleI AlfI AloI AlwNI ApaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI
Bme1580I BmgBI BmrI BpmI Bpu10I BsaAI BsaBI BsaHI
BsaXI BseYI BsiEI BsmI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssSI BstXI BstYI BtgI BtsI DrdI EaeI
EciI Eco57MI EcoO109I HaeII Hin4I HincII KasI MmeI
MscI MslI NaeI NarI NgoMIV NspI PfoI PmlI
PpiI PpuMI PspOMI PstI PvuI SalI SexAI SfcI
SfiI SfoI StuI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AcuI AfeI AflII AgeI AhdI
ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI
BciVI BclI BfrBI BglII BlpI BmtI BplI BpuEI
BsaI BsaWI BseRI BsgI BsiHKAI BsiWI BsmBI BspEI
BspHI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII EagI EarI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI MspA1I NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PshAI PsiI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SbfI ScaI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI