################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AvaI AviII AvrII BalI BanI BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstUI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoRV FauI FseI FspI HaeII HapII HgaI HpaI HpaII KasI KpnI KspI MfeI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI Psp1406I PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 15 GACGTC GACGT^C AciI 1192 GCGG CCGC(-3/-1) AcyI 15 GACGTC G[GA]^CG[CT]C AflIII 947 ACATGT A^C[GA][CT]GT AocI 868 CCTAAGG CC^T[ACGT]AGG AseI 1300 ATTAAT AT^TAAT AsnI 1300 ATTAAT AT^TAAT AspHI 894 GAGCTC G[AT]GC[AT]^C BanII 894 GAGCTC G[GA]GC[CT]^C BglI 1498 GCCTGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 894 GAGCTC G[AGT]GC[ACT]^C BpmI 899 CTGGAG CTGGAG(16/14) BsaBI 1515 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 15 GACGTC G[GA]^CG[CT]C BsgI 889 GTGCAG GTGCAG(16/14) BsiHKAI 894 GAGCTC G[AT]GC[AT]^C BsmFI 698 GGGAC GGGAC(10/14) Bsp1286I 894 GAGCTC G[AGT]GC[ACT]^C BspLU11I 947 ACATGT A^CATGT BsrDI 1209 CATTGC GCAATG(2/0) BstBI 776 TTCGAA TT^CGAA BstEII 1070 GGTTACC G^GT[ACGT]ACC BstPI 1070 GGTTACC G^GT[ACGT]ACC Bsu36I 868 CCTAAGG CC^T[ACGT]AGG CfoI 1407 GCGC GCG^C DsaI 1158 CCATGG C^C[GA][CT]GG Eco81I 868 CCTAAGG CC^T[ACGT]AGG EcoNI 864 CCTGCCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1070 GGTTACC G^GT[ACGT]ACC EcoRI 1343 GAATTC G^AATTC EcoT22I 1283 ATGCAT ATGCA^T HhaI 1407 GCGC GCG^C Hin1I 15 GACGTC G[GA]^CG[CT]C HinP1I 1407 GCGC G^CGC HincII 165 GTTGAC GT[CT]^[GA]AC HindII 165 GTTGAC GT[CT]^[GA]AC MaeII 16 ACGT A^CGT MamI 1515 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 1158 CCATGG C^CATGG NdeI 649 CATATG CA^TATG NsiI 1283 ATGCAT ATGCA^T NspI 947 ACATGT [GA]CATG^[CT] NspV 776 TTCGAA TT^CGAA PflMI 1467 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 15 GACGTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1300 ATTAAT AT^TAAT SacI 894 GAGCTC GAGCT^C SapI 130 GAAGAGC GCTCTTC(1/4) SfcI 366 CTACAG C^T[GA][CT]AG SfuI 776 TTCGAA TT^CGAA SspI 356 AATATT AAT^ATT Tsp45I 157 GTGAC ^GT[GC]AC Van91I 1467 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AspI 1257 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1306 GACCCTGTC AvaII 699 GGACC G^G[AT]CC 1256 GGACC BamHI 1163 GGATCC G^GATCC 1457 GGATCC BbvI 1234 GCAGC GCAGC(8/12) 1438 GCAGC BclI 609 TGATCA T^GATCA 938 TGATCA BfaI 79 CTAG C^TAG 721 CTAG BsmAI 745 GAGAC GTCTC(1/5) 3 GTCTC BsmI 1178 GCATTC GAATGC(1/-1) 1486 GCATTC Bsp1407I 509 TGTACA T^GTACA 1061 TGTACA BsrGI 509 TGTACA T^GTACA 1061 TGTACA BstF5I 1096 CATCC GGATG(2/0) 1476 GGATG EcoT14I 1158 CCATGG C^C[AT][AT]GG 1226 CCAAGG FbaI 609 TGATCA T^GATCA 938 TGATCA Fnu4HI 1234 GCAGC GC^[ACGT]GC 1438 GCAGC FokI 1096 CATCC GGATG(9/13) 1476 GGATG HaeIII 86 GGCC GG^CC 1497 GGCC HindIII 1109 AAGCTT A^AGCTT 1555 AAGCTT ItaI 1234 GCAGC GC^[ACGT]GC 1438 GCAGC MaeI 79 CTAG C^TAG 721 CTAG PflFI 1257 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1306 GACCCTGTC PleI 427 GAGTC GAGTC(4/5) 1433 GAGTC SspBI 509 TGTACA T^GTACA 1061 TGTACA StyI 1158 CCATGG C^C[AT][AT]GG 1226 CCAAGG TfiI 262 GATTC G^A[AT]TC 521 GATTC TseI 1234 GCAGC G^C[AT]GC 1438 GCAGC TspRI 844 CACTG CA[GC]TG[ACGT][ACGT]^ 1242 CAGTG Tth111I 1257 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1306 GACCCTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BbsI BpuAI BsaJI BsiYI BslI BspHI BsrI BssKI BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII HinfI HphI Ksp632I MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV RcaI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI Tru9I Tsp509I TthHB8I XhoII