(Circular) (Six-base) MAPSORT of: pFN21AE4507.seq Check: 8061 from: 1 to: 6295 pFN21AE4507 6295 bp With 182 enzymes: SgfI * March 28, 2012 14:06 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2024 4105 278 Size: 53 83 186 936 488 2081 2468 Fragments arranged by size: 2468 2081 936 488 186 83 53 AccI GT'mk_AC Cuts at: 1937 3638 1937 Size: 1701 4594 Acc65I G'GTAC_C Cuts at: 3616 3616 Size: 6295 AclI AA'CG_TT Cuts at: 1563 4422 4795 1563 Size: 2859 373 3063 Fragments arranged by size: 3063 2859 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2714 4358 5742 6010 1632 Size: 1082 1644 1384 268 1917 Fragments arranged by size: 1917 1644 1384 1082 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6098 AflIII A'CryG_T Cuts at: 1203 2952 5194 6171 1203 Size: 1749 2242 977 1327 Fragments arranged by size: 2242 1749 1327 977 AgeI A'CCGG_T Cuts at: 5126 5126 Size: 6295 AhdI GACnn_n'nnGTC Cuts at: 5024 5024 Size: 6295 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6295 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6295 AlwNI CAG_nnn'CTG Cuts at: 4038 5610 4038 Size: 1572 4723 ApaLI G'TGCA_C Cuts at: 4352 5508 4352 Size: 1156 5139 ApoI r'AATT_y Cuts at: 1493 2280 2682 2801 3348 3536 3604 3830 3982 Size: 787 402 119 547 188 68 226 152 Cuts at: 3982 6091 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 787 547 402 226 188 152 119 68 AseI AT'TA_AT Cuts at: 160 1032 3306 4849 160 Size: 872 2274 1543 1606 Fragments arranged by size: 2274 1606 1543 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6295 AvaI C'yCGr_G Cuts at: 1870 1943 3620 1870 Size: 73 1677 4545 Fragments arranged by size: 4545 1677 73 BamHI G'GATC_C Cuts at: 3168 3462 3625 3992 3168 Size: 294 163 367 5471 Fragments arranged by size: 5471 367 294 163 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3616 5071 618 Size: 325 217 627 1829 1455 1842 Fragments arranged by size: 1842 1829 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2903 3614 1391 Size: 1512 711 4072 Fragments arranged by size: 4072 1512 711 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2129 2513 2737 3382 928 Size: 971 230 384 224 645 3841 Fragments arranged by size: 3841 971 645 384 230 224 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4486 4520 1903 Size: 34 2549 34 3678 Fragments arranged by size: 3678 2549 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4187 5397 4187 Size: 1210 5085 BclI T'GATC_A Cuts at: 1548 2614 2943 1548 Size: 1066 329 4900 Fragments arranged by size: 4900 1066 329 BfrBI ATG'CAT Cuts at: 3290 3290 Size: 6295 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3509 4906 136 Size: 107 122 71 631 605 1837 1397 1525 Fragments arranged by size: 1837 1525 1397 631 605 122 107 71 BglII A'GATC_T Cuts at: 6290 6290 Size: 6295 BlpI GC'TnA_GC Cuts at: 3712 3712 Size: 6295 Bme1580I G_kGCm'C Cuts at: 1790 4356 5512 1790 Size: 2566 1156 2573 Fragments arranged by size: 2573 2566 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6295 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4026 4984 442 Size: 416 53 83 399 24 2609 958 1753 Fragments arranged by size: 2609 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6295 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3260 3299 3260 Size: 39 6256 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2925 4955 1239 Size: 1686 2030 2579 Fragments arranged by size: 2579 2030 1686 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4420 5285 5583 4420 Size: 865 298 5132 Fragments arranged by size: 5132 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4958 882 Size: 617 3459 2219 Fragments arranged by size: 3459 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4086 493 Size: 711 2882 2702 Fragments arranged by size: 2882 2702 711 BsaBI GATnn'nnATC Cuts at: 3524 6289 3524 Size: 2765 3530 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2021 4102 4484 5119 Size: 53 83 186 936 488 2081 382 635 Cuts at: 5119 275 Size: 1451 Fragments arranged by size: 2081 1451 936 635 488 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4728 5126 5400 5547 5958 4728 Size: 398 274 147 411 5065 Fragments arranged by size: 5065 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6070 Fragments arranged by size: 6070 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6295 BseYI C'CCAG_C Cuts at: 5498 5994 5498 Size: 496 5799 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2915 1163 Size: 573 1179 4543 Fragments arranged by size: 4543 1179 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3745 4506 5534 1540 Size: 275 186 1744 761 1028 2301 Fragments arranged by size: 2301 1744 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2903 4356 4441 5512 2903 Size: 1453 85 1071 3686 Fragments arranged by size: 3686 1453 1071 85 BsmI GAATG_Cn' Cuts at: 3183 3491 3806 3899 3183 Size: 308 315 93 5579 Fragments arranged by size: 5579 315 308 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2903 3614 4356 4441 5512 1391 Size: 399 1113 711 742 85 1071 2174 Fragments arranged by size: 2174 1113 1071 742 711 399 85 BspEI T'CCGG_A Cuts at: 5958 5958 Size: 6295 BspHI T'CATG_A Cuts at: 2155 3333 3355 4184 5914 2155 Size: 1178 22 829 1730 2536 Fragments arranged by size: 2536 1730 1178 829 22 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3650 844 Size: 2806 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4182 1394 Size: 429 2359 3507 Fragments arranged by size: 3507 2359 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3213 4790 4964 65 Size: 1183 1965 1577 174 1396 Fragments arranged by size: 1965 1577 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4939 5126 1700 Size: 110 3129 187 2869 Fragments arranged by size: 3129 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2514 3066 96 Size: 2418 552 3325 Fragments arranged by size: 3325 2418 552 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6295 BssSI C'ACGA_G Cuts at: 4355 5367 4355 Size: 1012 5283 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6295 BstBI TT'CG_AA Cuts at: 2782 5099 2782 Size: 2317 3978 BstEII G'GTnAC_C Cuts at: 1184 3075 1184 Size: 1891 4404 BstYI r'GATC_y Cuts at: 1972 2997 3168 3462 3625 3992 4377 4394 5846 Size: 1025 171 294 163 367 385 17 1452 Cuts at: 5846 5932 5944 6290 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1025 385 367 346 294 171 163 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2874 2874 Size: 6295 BtgI C'CryG_G Cuts at: 513 1065 3163 513 Size: 552 2098 3645 Fragments arranged by size: 3645 2098 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5556 Fragments arranged by size: 5556 683 56 BtsI GCAGTG_nn' Cuts at: 3811 4605 4625 3811 Size: 794 20 5481 Fragments arranged by size: 5481 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6295 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6260 DraI TTT'AAA Cuts at: 3599 3599 Size: 6295 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5302 809 Size: 507 3986 1802 Fragments arranged by size: 3986 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3742 4631 6260 Size: 54 1425 50 275 18 1912 889 1629 Cuts at: 6260 8 Size: 43 Fragments arranged by size: 1912 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3742 1537 Size: 275 1930 4090 Fragments arranged by size: 4090 1930 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2130 2277 2880 4225 1353 Size: 777 147 603 1345 3423 Fragments arranged by size: 3423 1345 777 603 147 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4882 5256 5402 387 Size: 1064 138 3293 374 146 1280 Fragments arranged by size: 3293 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2901 2901 Size: 6295 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2714 2925 4358 4955 5742 6010 1239 Size: 393 1082 211 1433 597 787 268 1524 Fragments arranged by size: 1524 1433 1082 787 597 393 268 211 EcoNI CCTnn'n_nnAGG Cuts at: 2873 2873 Size: 6295 EcoRI G'AATT_C Cuts at: 3348 3604 3982 3348 Size: 256 378 5661 Fragments arranged by size: 5661 378 256 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2288 2288 Size: 6295 FspI TGC'GCA Cuts at: 4801 4801 Size: 6295 HaeII r_GCGC'y Cuts at: 1678 5442 1678 Size: 3764 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4936 4968 5010 5042 1373 Size: 32 135 32 3364 32 42 32 2626 Fragments arranged by size: 3364 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2172 3639 3885 669 Size: 988 281 234 1467 246 3079 Fragments arranged by size: 3079 1467 988 281 246 234 HindIII A'AGCT_T Cuts at: 748 3114 3560 748 Size: 2366 446 3483 Fragments arranged by size: 3483 2366 446 HpaI GTT'AAC Cuts at: 3885 3885 Size: 6295 KpnI G_GTAC'C Cuts at: 3620 3620 Size: 6295 MfeI C'AATT_G Cuts at: 3894 3894 Size: 6295 MluI A'CGCG_T Cuts at: 6171 6171 Size: 6295 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2818 5409 5593 1305 Size: 1513 2591 184 2007 Fragments arranged by size: 2591 2007 1513 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4473 Fragments arranged by size: 4473 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4253 4612 4771 518 Size: 648 3087 359 159 2042 Fragments arranged by size: 3087 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3711 4388 5536 5781 1606 Size: 2105 677 1148 245 2120 Fragments arranged by size: 2120 2105 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 3163 513 Size: 552 2098 3645 Fragments arranged by size: 3645 2098 552 NdeI CA'TA_TG Cuts at: 387 2655 387 Size: 2268 4027 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6295 NotI GC'GGCC_GC Cuts at: 3742 3742 Size: 6295 NsiI A_TGCA'T Cuts at: 3292 3292 Size: 6295 NspI r_CATG'y Cuts at: 1132 1229 2956 3653 5198 1132 Size: 97 1727 697 1545 2229 Fragments arranged by size: 2229 1727 1545 697 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6295 PciI A'CATG_T Cuts at: 2952 5194 2952 Size: 2242 4053 PflMI CCAn_nnn'nTGG Cuts at: 1108 3478 1108 Size: 2370 3925 PfoI T'CCnGG_A Cuts at: 2130 2130 Size: 6295 PmeI GTTT'AAAC Cuts at: 3599 3599 Size: 6295 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4364 4396 5877 5909 4364 Size: 32 1481 32 4750 Fragments arranged by size: 4750 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2024 1613 Size: 411 5884 PsiI TTA'TAA Cuts at: 3122 3865 3122 Size: 743 5552 PstI C_TGCA'G Cuts at: 830 3647 830 Size: 2817 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6295 SacI G_AGCT'C Cuts at: 2903 2903 Size: 6295 SalI G'TCGA_C Cuts at: 1936 3637 1936 Size: 1701 4594 SapI GCTCTTCn'nnn_ Cuts at: 2130 2130 Size: 6295 SbfI CC_TGCA'GG Cuts at: 3647 3647 Size: 6295 ScaI AGT'ACT Cuts at: 4543 6255 4543 Size: 1712 4583 SfcI C'TryA_G Cuts at: 826 1045 2371 3643 4778 5459 5650 826 Size: 219 1326 1272 1135 681 191 1471 Fragments arranged by size: 1471 1326 1272 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6295 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6295 SmaI CCC'GGG Cuts at: 1872 3622 1872 Size: 1750 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4399 5300 5562 820 Size: 197 926 2456 901 262 1553 Fragments arranged by size: 2456 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4086 493 Size: 3593 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6295 SphI G_CATG'C Cuts at: 1132 3653 1132 Size: 2521 3774 SspI AAT'ATT Cuts at: 5 52 2363 4219 5 Size: 47 2311 1856 2081 Fragments arranged by size: 2311 2081 1856 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6295 StyI C'CwwG_G Cuts at: 513 1065 3163 3231 3734 513 Size: 552 2098 68 503 3074 Fragments arranged by size: 3074 2098 552 503 68 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4322 4339 4492 4677 5122 325 Size: 1255 2742 17 153 185 445 1498 Fragments arranged by size: 2742 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2514 3066 4541 Size: 274 80 33 51 1443 537 552 1475 Cuts at: 4541 6253 96 Size: 1712 138 Fragments arranged by size: 1712 1475 1443 552 537 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2287 2319 6255 6287 2287 Size: 32 3936 32 2295 Fragments arranged by size: 3936 2295 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3265 3314 1316 Size: 1949 49 4297 Fragments arranged by size: 4297 1949 49 XbaI T'CTAG_A Cuts at: 3631 3631 Size: 6295 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6295 XmaI C'CCGG_G Cuts at: 1870 3620 1870 Size: 1750 4545 XmnI GAAnn'nnTTC Cuts at: 4424 4424 Size: 6295 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2022 4103 276 Size: 53 83 186 936 488 2081 2468 Fragments arranged by size: 2468 2081 936 488 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI